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The map label for this gene is ispB [H]

Identifier: 159184398

GI number: 159184398

Start: 622765

End: 623781

Strand: Reverse

Name: ispB [H]

Synonym: Atu0634

Alternate gene names: 159184398

Gene position: 623781-622765 (Counterclockwise)

Preceding gene: 159184404

Following gene: 159184397

Centisome position: 21.95

GC content: 60.77

Gene sequence:

>1017_bases
TTGGGCGTCGTCATACCGCTTGAAGAAAGCAAAAACAAACTCGCATCCGTCAAGCCGCTTGTCGACCTGACCCGTCCCGA
CATGGAACGGGTGAACCAGCTCATCCTTTCCAGGGCCGGTTCCGATGTCCAGATGATACCCGAGGTGGCCAACCATCTTA
TCTCCTCCGGCGGCAAACGCCTGCGGCCGATGCTGACGCTCGCCTCGGCCTCGATGTTCGGTTATGAGGGAGATAACCAC
ATCAAGCTCGCCACCAGCGTGGAGTTCATGCATACGGCGACGCTTCTGCACGACGATGTGGTGGATGAAAGCGATCTGCG
TCGCGGCAAATCCACCGCCCGCACCATCTGGGGCAACCAGGCGAGCGTTCTCGTCGGCGACTTCCTGCTCGGCCAGGCTT
TCCGCATGATGGTGGATGTCGGTTCGCTGGATGCGCTGGATGTGCTCTCCACCGCCGCTTCCGTCATCGCCGAGGGCGAG
GTGCTGCAGCTTTCGGTCGCCAAGAACATGGAAACGACCGAGGACGATTACCTGCAGGTGATTCGCGCCAAGACGGCCGC
CCTCTTTGCCGCCGCGGCCGAAGTCGGCCCCATTGTCGCCAAGACGGACAAGGCGAGCCGCAGCGCGCTGAAATCCTATG
GCATGAATCTCGGCCTCGCTTTCCAGCTGGTCGACGACGTGCTGGACTATGGCGGCAAGTCCGCCGATCTCGGCAAGAAC
ACCGGTGACGATTTCCGCGAGGGCAAGATCACGCTGCCGGTCATCCTGTCTTATCGCCGCGGCACGCAGGATGAGCGCGC
CTTCTGGCGCAACGCCATCGAAAAGGGCGAAAGCAGCGACGAGAACCTGGAAAAGGCTCTTGGTCTGATCACCAAGTATA
ACGGCTTGGGCGACACGATCGGTCGCGCCACACATTACGGCACGATTGCCCGTGACGCGCTTGCGCCGTTGCCGCAGAGC
CCATGGAAAAATGCACTTCTCGAGGTCATCGACTTCTGCATCGAGCGTCTCAACTGA

Upstream 100 bases:

>100_bases
AAATTCCCATCTTTTTGAATGGCTAGCGGCAATTCGCCCCTTGCCGCACAAGGCATCAGTTTTTATTGTCGAGTTCAAAT
TCGAATTGGAGTCCTGTCCC

Downstream 100 bases:

>100_bases
CGCTCAAGCCCTAGGCATAAGGCGGATGGACAGGCCCATCCGCCCGACGTTCCGGGATGGTTTCTCCGCAAGGCGTGATT
GTGGCACGGGCCATAATTTC

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MGVVIPLEESKNKLASVKPLVDLTRPDMERVNQLILSRAGSDVQMIPEVANHLISSGGKRLRPMLTLASASMFGYEGDNH
IKLATSVEFMHTATLLHDDVVDESDLRRGKSTARTIWGNQASVLVGDFLLGQAFRMMVDVGSLDALDVLSTAASVIAEGE
VLQLSVAKNMETTEDDYLQVIRAKTAALFAAAAEVGPIVAKTDKASRSALKSYGMNLGLAFQLVDDVLDYGGKSADLGKN
TGDDFREGKITLPVILSYRRGTQDERAFWRNAIEKGESSDENLEKALGLITKYNGLGDTIGRATHYGTIARDALAPLPQS
PWKNALLEVIDFCIERLN

Sequences:

>Translated_338_residues
MGVVIPLEESKNKLASVKPLVDLTRPDMERVNQLILSRAGSDVQMIPEVANHLISSGGKRLRPMLTLASASMFGYEGDNH
IKLATSVEFMHTATLLHDDVVDESDLRRGKSTARTIWGNQASVLVGDFLLGQAFRMMVDVGSLDALDVLSTAASVIAEGE
VLQLSVAKNMETTEDDYLQVIRAKTAALFAAAAEVGPIVAKTDKASRSALKSYGMNLGLAFQLVDDVLDYGGKSADLGKN
TGDDFREGKITLPVILSYRRGTQDERAFWRNAIEKGESSDENLEKALGLITKYNGLGDTIGRATHYGTIARDALAPLPQS
PWKNALLEVIDFCIERLN
>Mature_337_residues
GVVIPLEESKNKLASVKPLVDLTRPDMERVNQLILSRAGSDVQMIPEVANHLISSGGKRLRPMLTLASASMFGYEGDNHI
KLATSVEFMHTATLLHDDVVDESDLRRGKSTARTIWGNQASVLVGDFLLGQAFRMMVDVGSLDALDVLSTAASVIAEGEV
LQLSVAKNMETTEDDYLQVIRAKTAALFAAAAEVGPIVAKTDKASRSALKSYGMNLGLAFQLVDDVLDYGGKSADLGKNT
GDDFREGKITLPVILSYRRGTQDERAFWRNAIEKGESSDENLEKALGLITKYNGLGDTIGRATHYGTIARDALAPLPQSP
WKNALLEVIDFCIERLN

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=296, Percent_Identity=28.7162162162162, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI4758430, Length=213, Percent_Identity=26.7605633802817, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI83700220, Length=213, Percent_Identity=26.7605633802817, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=46.1059190031153, Blast_Score=281, Evalue=3e-77,
Organism=Escherichia coli, GI1786623, Length=241, Percent_Identity=35.2697095435685, Blast_Score=101, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17505681, Length=244, Percent_Identity=31.5573770491803, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319475, Length=293, Percent_Identity=30.0341296928328, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325188, Length=222, Percent_Identity=23.4234234234234, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24651612, Length=311, Percent_Identity=30.8681672025724, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI281365769, Length=303, Percent_Identity=26.4026402640264, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24660002, Length=303, Percent_Identity=26.4026402640264, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36682; Mature: 36551

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVVIPLEESKNKLASVKPLVDLTRPDMERVNQLILSRAGSDVQMIPEVANHLISSGGKR
CCEEEECHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
LRPMLTLASASMFGYEGDNHIKLATSVEFMHTATLLHDDVVDESDLRRGKSTARTIWGNQ
HHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHCCCC
ASVLVGDFLLGQAFRMMVDVGSLDALDVLSTAASVIAEGEVLQLSVAKNMETTEDDYLQV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
IRAKTAALFAAAAEVGPIVAKTDKASRSALKSYGMNLGLAFQLVDDVLDYGGKSADLGKN
HHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
TGDDFREGKITLPVILSYRRGTQDERAFWRNAIEKGESSDENLEKALGLITKYNGLGDTI
CCCCHHCCCEEEHEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHH
GRATHYGTIARDALAPLPQSPWKNALLEVIDFCIERLN
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GVVIPLEESKNKLASVKPLVDLTRPDMERVNQLILSRAGSDVQMIPEVANHLISSGGKR
CEEEECHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
LRPMLTLASASMFGYEGDNHIKLATSVEFMHTATLLHDDVVDESDLRRGKSTARTIWGNQ
HHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHCCCC
ASVLVGDFLLGQAFRMMVDVGSLDALDVLSTAASVIAEGEVLQLSVAKNMETTEDDYLQV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
IRAKTAALFAAAAEVGPIVAKTDKASRSALKSYGMNLGLAFQLVDDVLDYGGKSADLGKN
HHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
TGDDFREGKITLPVILSYRRGTQDERAFWRNAIEKGESSDENLEKALGLITKYNGLGDTI
CCCCHHCCCEEEHEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHH
GRATHYGTIARDALAPLPQSPWKNALLEVIDFCIERLN
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]