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The map label for this gene is rhtB [C]

Identifier: 159184252

GI number: 159184252

Start: 306202

End: 306819

Strand: Reverse

Name: rhtB [C]

Synonym: Atu0313

Alternate gene names: 159184252

Gene position: 306819-306202 (Counterclockwise)

Preceding gene: 15887664

Following gene: 15887662

Centisome position: 10.8

GC content: 60.36

Gene sequence:

>618_bases
ATGACGCTGACGACCCTTCTAGCCTATGCCACTGCCCTCTTCATCGCCGCTGCCATTCCCGGCCCCGGCATGACGGCGAT
CGTGGCGCGGGCGCTGGGTTCGGGCTTCCGGGAAACCTTCTTCATGGGCCTCGGGCTGGTGCTGGGCGATATGATCTACC
TGACCGGCGTGATACTCGGCCTCGCCTTCGTGGCACAGACCTTTCAGGAGGCCTTCATGGTCCTGAAATTCGCTGGTGCG
GCTTATCTTCTCTATATCGCCTGGAAGCTCTGGACTGCCGGCCTCTTGCCGCAGGACCTGAAGGCGAGAAAAAGCACCAG
CATACCTATGTCGTTTCTGTCCGGCTTACTGATCACGCTCGGTAATCCAAAAACGATGCTGTTTTATGTGGCGCTGGTGC
CGACGCTGATCGACATCCGCATGATCGGCCCGTCGGAATATGCGACACTGCTGGCGCTGACCTTCGTCGTGCTGATGGCC
GTTCTCCTGCCGTACATCCTGCTTGCGGCCAAGGCACGCAATCTCTTGAAACGGCCGAGCGCCTTGACGATCCTCAACCG
CACGGCGGCGGGCATTCTGGCCGGAACGGCGACGATGATCGCAATCCGCAGCACCTGA

Upstream 100 bases:

>100_bases
GTTTCGGTTCGACCGGGGTGTAGCCGACAAGCAGCATGATCGATTGTTTTTACCCGGCAATTTCGGTATTGATCGAGCGG
ATTTTCCGGGAAAAACGATC

Downstream 100 bases:

>100_bases
AATAAATTCATACGCCTGCCCCATTTTCCGTAAAATTATTCCGGTCATTTCGCGATATTAGGACGAACGTTCGCTAGCCT
GAGAGGGCTATTGCTCCTAT

Product: RhtB family transporter

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTLTTLLAYATALFIAAAIPGPGMTAIVARALGSGFRETFFMGLGLVLGDMIYLTGVILGLAFVAQTFQEAFMVLKFAGA
AYLLYIAWKLWTAGLLPQDLKARKSTSIPMSFLSGLLITLGNPKTMLFYVALVPTLIDIRMIGPSEYATLLALTFVVLMA
VLLPYILLAAKARNLLKRPSALTILNRTAAGILAGTATMIAIRST

Sequences:

>Translated_205_residues
MTLTTLLAYATALFIAAAIPGPGMTAIVARALGSGFRETFFMGLGLVLGDMIYLTGVILGLAFVAQTFQEAFMVLKFAGA
AYLLYIAWKLWTAGLLPQDLKARKSTSIPMSFLSGLLITLGNPKTMLFYVALVPTLIDIRMIGPSEYATLLALTFVVLMA
VLLPYILLAAKARNLLKRPSALTILNRTAAGILAGTATMIAIRST
>Mature_204_residues
TLTTLLAYATALFIAAAIPGPGMTAIVARALGSGFRETFFMGLGLVLGDMIYLTGVILGLAFVAQTFQEAFMVLKFAGAA
YLLYIAWKLWTAGLLPQDLKARKSTSIPMSFLSGLLITLGNPKTMLFYVALVPTLIDIRMIGPSEYATLLALTFVVLMAV
LLPYILLAAKARNLLKRPSALTILNRTAAGILAGTATMIAIRST

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=191, Percent_Identity=34.0314136125654, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786522, Length=186, Percent_Identity=28.494623655914, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21881; Mature: 21750

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTTLLAYATALFIAAAIPGPGMTAIVARALGSGFRETFFMGLGLVLGDMIYLTGVILG
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFVAQTFQEAFMVLKFAGAAYLLYIAWKLWTAGLLPQDLKARKSTSIPMSFLSGLLITL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHC
GNPKTMLFYVALVPTLIDIRMIGPSEYATLLALTFVVLMAVLLPYILLAAKARNLLKRPS
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALTILNRTAAGILAGTATMIAIRST
HHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
TLTTLLAYATALFIAAAIPGPGMTAIVARALGSGFRETFFMGLGLVLGDMIYLTGVILG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFVAQTFQEAFMVLKFAGAAYLLYIAWKLWTAGLLPQDLKARKSTSIPMSFLSGLLITL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHC
GNPKTMLFYVALVPTLIDIRMIGPSEYATLLALTFVVLMAVLLPYILLAAKARNLLKRPS
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALTILNRTAAGILAGTATMIAIRST
HHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]