Click here to switch to the map view.

The map label for this gene is dut

Identifier: 15887664

GI number: 15887664

Start: 306897

End: 307367

Strand: Reverse

Name: dut

Synonym: Atu0314

Alternate gene names: 15887664

Gene position: 307367-306897 (Counterclockwise)

Preceding gene: 159184254

Following gene: 159184252

Centisome position: 10.82

GC content: 62.21

Gene sequence:

>471_bases
ATGACCGTTCAAAACGACAATCGACCCCTCCTCCGGCTCGTGCGTCTCGCCAATGGCGCAGACCTCGAATTGCCATCCTA
TGAAACCCGTGGCGCCGCCGGCATGGACCTGCGCGCCGCAGTGCCGGCCGATGAACCGCTGAATTTGCAGCCGGGCGAAC
GGGCTCTGGTGCCGACAGGTTTCATCTTCGAAGTTCCGCAAGGCTATGAGGCGCAAATCCGTCCGCGCTCCGGTCTTGCC
ATCAAGAACGGCATCACCTGCCTCAACTCCCCCGGCACGGTGGACAGCGATTATCGCGGCGAAGTGAAGGTCATCCTCGC
CAATCTGGGGCAGGATGATTTCACCATCGAACGCGGCATGCGTATTGCGCAGATGGTGATCGCGCCGGTGACGCAGGTTA
CTGTGAGCGAAGTGACGGAAACCTCCGAGACGGCGCGCGGTGCCGGCGGTTTCGGTTCGACCGGGGTGTAG

Upstream 100 bases:

>100_bases
GTTTCGGCCATCTGACAAATCCTGTGAATTGCGGGCTATCTAGCGTTTCAGGCCCGCATTTGCAATTGACCTCGCCGCGA
TCTCGGCAAACTAATGCCGC

Downstream 100 bases:

>100_bases
CCGACAAGCAGCATGATCGATTGTTTTTACCCGGCAATTTCGGTATTGATCGAGCGGATTTTCCGGGAAAAACGATCATG
ACGCTGACGACCCTTCTAGC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTVQNDNRPLLRLVRLANGADLELPSYETRGAAGMDLRAAVPADEPLNLQPGERALVPTGFIFEVPQGYEAQIRPRSGLA
IKNGITCLNSPGTVDSDYRGEVKVILANLGQDDFTIERGMRIAQMVIAPVTQVTVSEVTETSETARGAGGFGSTGV

Sequences:

>Translated_156_residues
MTVQNDNRPLLRLVRLANGADLELPSYETRGAAGMDLRAAVPADEPLNLQPGERALVPTGFIFEVPQGYEAQIRPRSGLA
IKNGITCLNSPGTVDSDYRGEVKVILANLGQDDFTIERGMRIAQMVIAPVTQVTVSEVTETSETARGAGGFGSTGV
>Mature_155_residues
TVQNDNRPLLRLVRLANGADLELPSYETRGAAGMDLRAAVPADEPLNLQPGERALVPTGFIFEVPQGYEAQIRPRSGLAI
KNGITCLNSPGTVDSDYRGEVKVILANLGQDDFTIERGMRIAQMVIAPVTQVTVSEVTETSETARGAGGFGSTGV

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=145, Percent_Identity=39.3103448275862, Blast_Score=94, Evalue=7e-20,
Organism=Homo sapiens, GI4503423, Length=145, Percent_Identity=38.6206896551724, Blast_Score=92, Evalue=1e-19,
Organism=Homo sapiens, GI70906441, Length=145, Percent_Identity=38.6206896551724, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI1790071, Length=138, Percent_Identity=44.2028985507246, Blast_Score=116, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71988561, Length=135, Percent_Identity=43.7037037037037, Blast_Score=105, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6319729, Length=158, Percent_Identity=39.873417721519, Blast_Score=97, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24583610, Length=147, Percent_Identity=39.4557823129252, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19921126, Length=147, Percent_Identity=39.4557823129252, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_AGRT5 (Q8UII1)

Other databases:

- EMBL:   AE007869
- PIR:   A97397
- PIR:   AB2615
- RefSeq:   NP_353345.1
- ProteinModelPortal:   Q8UII1
- SMR:   Q8UII1
- STRING:   Q8UII1
- GeneID:   1132352
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0314
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   Q8UII1
- ProtClustDB:   PRK00601
- BioCyc:   ATUM176299-1:ATU0314-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16599; Mature: 16468

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: BINDING 89-89 BINDING 103-103

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQNDNRPLLRLVRLANGADLELPSYETRGAAGMDLRAAVPADEPLNLQPGERALVPTG
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEECCCC
FIFEVPQGYEAQIRPRSGLAIKNGITCLNSPGTVDSDYRGEVKVILANLGQDDFTIERGM
EEEECCCCCCEEECCCCCEEECCCEEEECCCCCCCCCCCCEEEEEEEECCCCCEEHHHHH
RIAQMVIAPVTQVTVSEVTETSETARGAGGFGSTGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TVQNDNRPLLRLVRLANGADLELPSYETRGAAGMDLRAAVPADEPLNLQPGERALVPTG
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEECCCC
FIFEVPQGYEAQIRPRSGLAIKNGITCLNSPGTVDSDYRGEVKVILANLGQDDFTIERGM
EEEECCCCCCEEECCCCCEEECCCEEEECCCCCCCCCCCCEEEEEEEECCCCCEEHHHHH
RIAQMVIAPVTQVTVSEVTETSETARGAGGFGSTGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194