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The map label for this gene is dgoD [H]

Identifier: 159184240

GI number: 159184240

Start: 266640

End: 267842

Strand: Reverse

Name: dgoD [H]

Synonym: Atu0270

Alternate gene names: 159184240

Gene position: 267842-266640 (Counterclockwise)

Preceding gene: 15887621

Following gene: 15887618

Centisome position: 9.43

GC content: 60.35

Gene sequence:

>1203_bases
ATGAAAATCACAAAACTCGAGACCGTCCGTGTGGCGGAACGGACGAACCTGCTCTGGGTTCTGGTCCATACGGATGAGGG
CATTACCGGGCTCGGCGAAACCTTCTTCGGAGCGGAGACGGTGGAGACCTATGTGCATGAATATATCGCCCCCCGCGTGA
TCGGCCGCGATCCGCTGCAGATCGACCTGCTGGCTCAGGACCTTGTCGGTTATCTCGGATTCCGTTCATCGGGAGCGGAA
GTGCGCGGCAATTCAGCCTTCGATATCGCGCTGTGGGACATTTTCGGCAAGGCCACCAACCAGCCTATCGCCCAGCTGCT
CGGCGGCTTCAGCCGCAGGGAAATCCGCACCTACAATACCTGCGCGGGCACCGAATATATCAAGAAGGCCACCGGCCAGC
AGACGGCTAATTATGGCCTCTCCGGCGGCAAGGACTATGATGATCTGAACGGCTTCCTGCATAGGGCGGACGAGCTTGCC
CATTCGCTGCTGGAAGACGGCATCACCGCCATGAAGATCTGGCCGTTTGATGCGGCGGCGGAAAAGACGCGCGGGCAATA
TATCTCGATGCCGGACCTGAAAAGCGCGCTCGAGCCGTTTGAAAAAATCCGCAAGGCGGTGGGCGACAAGATGGATATCA
TGGTGGAGTTCCACTCCATGTGGCAGCTTCTGCCCGCCATGCAGATCGCCAAGGCGCTTACCCCCTACCAGACCTTCTGG
CATGAAGATCCGATCAAGATGGATAGCCTTTCCAGCCTGACACGGTATGCCGCCGTGTCGCCCGCACCGATTTCCGCGTC
CGAAACGCTCGGTTCCCGCTGGGCTTTCCGCGATCTCCTGGAAACGGGTGCTGCCGGTGTTGTCATGCTTGACATCAGCT
GGTGCGGCGGCCTTTCGGAAGCGCGCAAGATCGCCTCCATGGCGGAAGCCTGGCACCTGCCGGTGGCGCCGCACGATTGC
ACGGGGCCGGTGGTGCTCTGCGCCTCCACGCATCTGTCCCTCAATGCGCCGAATGCGCTGGTGCAGGAAAGCGTGCGCGC
CTTCTACAAGACCTGGTATCGCGATCTCGTCACCGCTTTGCCCGAGGTGAAAAACGGCATGATCACCGTGCCGCCGGGTG
CTGGCCTCGGCATGGAGCTGCATCCAGATATCGAAAAGACCTTCACCGTCAGCCGCCGTTTTTCGGATGCGGCGTCGATC
TGA

Upstream 100 bases:

>100_bases
GGCATTTCGATGAAAGTGTGCATGCACTTCTGGCAGCCGGCATCGCCTGATTTTTTCAACGCAGTCCCAGATGGAAACGA
CTGCCTCTGGAGGAGACACC

Downstream 100 bases:

>100_bases
TCAGAACAGGTTGCCCTGATTACCCGGGTCAGAAGAAGCCGGTTTCGCCGGCTTCTTTTTTGGCGCGGCGGCGGTCTCCG
GGGCGGGTGTCCCCTCTCCC

Product: mandelate racemase / muconate lactonizing enzyme family protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKITKLETVRVAERTNLLWVLVHTDEGITGLGETFFGAETVETYVHEYIAPRVIGRDPLQIDLLAQDLVGYLGFRSSGAE
VRGNSAFDIALWDIFGKATNQPIAQLLGGFSRREIRTYNTCAGTEYIKKATGQQTANYGLSGGKDYDDLNGFLHRADELA
HSLLEDGITAMKIWPFDAAAEKTRGQYISMPDLKSALEPFEKIRKAVGDKMDIMVEFHSMWQLLPAMQIAKALTPYQTFW
HEDPIKMDSLSSLTRYAAVSPAPISASETLGSRWAFRDLLETGAAGVVMLDISWCGGLSEARKIASMAEAWHLPVAPHDC
TGPVVLCASTHLSLNAPNALVQESVRAFYKTWYRDLVTALPEVKNGMITVPPGAGLGMELHPDIEKTFTVSRRFSDAASI

Sequences:

>Translated_400_residues
MKITKLETVRVAERTNLLWVLVHTDEGITGLGETFFGAETVETYVHEYIAPRVIGRDPLQIDLLAQDLVGYLGFRSSGAE
VRGNSAFDIALWDIFGKATNQPIAQLLGGFSRREIRTYNTCAGTEYIKKATGQQTANYGLSGGKDYDDLNGFLHRADELA
HSLLEDGITAMKIWPFDAAAEKTRGQYISMPDLKSALEPFEKIRKAVGDKMDIMVEFHSMWQLLPAMQIAKALTPYQTFW
HEDPIKMDSLSSLTRYAAVSPAPISASETLGSRWAFRDLLETGAAGVVMLDISWCGGLSEARKIASMAEAWHLPVAPHDC
TGPVVLCASTHLSLNAPNALVQESVRAFYKTWYRDLVTALPEVKNGMITVPPGAGLGMELHPDIEKTFTVSRRFSDAASI
>Mature_400_residues
MKITKLETVRVAERTNLLWVLVHTDEGITGLGETFFGAETVETYVHEYIAPRVIGRDPLQIDLLAQDLVGYLGFRSSGAE
VRGNSAFDIALWDIFGKATNQPIAQLLGGFSRREIRTYNTCAGTEYIKKATGQQTANYGLSGGKDYDDLNGFLHRADELA
HSLLEDGITAMKIWPFDAAAEKTRGQYISMPDLKSALEPFEKIRKAVGDKMDIMVEFHSMWQLLPAMQIAKALTPYQTFW
HEDPIKMDSLSSLTRYAAVSPAPISASETLGSRWAFRDLLETGAAGVVMLDISWCGGLSEARKIASMAEAWHLPVAPHDC
TGPVVLCASTHLSLNAPNALVQESVRAFYKTWYRDLVTALPEVKNGMITVPPGAGLGMELHPDIEKTFTVSRRFSDAASI

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=406, Percent_Identity=30.2955665024631, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787864, Length=406, Percent_Identity=26.8472906403941, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44048; Mature: 44048

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITKLETVRVAERTNLLWVLVHTDEGITGLGETFFGAETVETYVHEYIAPRVIGRDPLQ
CCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCC
IDLLAQDLVGYLGFRSSGAEVRGNSAFDIALWDIFGKATNQPIAQLLGGFSRREIRTYNT
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHCCCCCCHHHHHHCCCCHHHHHCCCC
CAGTEYIKKATGQQTANYGLSGGKDYDDLNGFLHRADELAHSLLEDGITAMKIWPFDAAA
HHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
EKTRGQYISMPDLKSALEPFEKIRKAVGDKMDIMVEFHSMWQLLPAMQIAKALTPYQTFW
HHHCCCEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
HEDPIKMDSLSSLTRYAAVSPAPISASETLGSRWAFRDLLETGAAGVVMLDISWCGGLSE
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCHHH
ARKIASMAEAWHLPVAPHDCTGPVVLCASTHLSLNAPNALVQESVRAFYKTWYRDLVTAL
HHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHH
PEVKNGMITVPPGAGLGMELHPDIEKTFTVSRRFSDAASI
HHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKITKLETVRVAERTNLLWVLVHTDEGITGLGETFFGAETVETYVHEYIAPRVIGRDPLQ
CCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCC
IDLLAQDLVGYLGFRSSGAEVRGNSAFDIALWDIFGKATNQPIAQLLGGFSRREIRTYNT
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHCCCCCCHHHHHHCCCCHHHHHCCCC
CAGTEYIKKATGQQTANYGLSGGKDYDDLNGFLHRADELAHSLLEDGITAMKIWPFDAAA
HHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHH
EKTRGQYISMPDLKSALEPFEKIRKAVGDKMDIMVEFHSMWQLLPAMQIAKALTPYQTFW
HHHCCCEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
HEDPIKMDSLSSLTRYAAVSPAPISASETLGSRWAFRDLLETGAAGVVMLDISWCGGLSE
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCHHH
ARKIASMAEAWHLPVAPHDCTGPVVLCASTHLSLNAPNALVQESVRAFYKTWYRDLVTAL
HHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHH
PEVKNGMITVPPGAGLGMELHPDIEKTFTVSRRFSDAASI
HHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]