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The map label for this gene is lutR [H]

Identifier: 15887621

GI number: 15887621

Start: 267893

End: 268600

Strand: Reverse

Name: lutR [H]

Synonym: Atu0271

Alternate gene names: 15887621

Gene position: 268600-267893 (Counterclockwise)

Preceding gene: 159184241

Following gene: 159184240

Centisome position: 9.45

GC content: 61.16

Gene sequence:

>708_bases
ATGTTAAACGCCATTTCGCATGATTCCGGCCTCGTCAGCAGCACGATCGGCGCGATCACCCGCCATATCCGCGAAAACGA
GCTGGCGCCCGGCGCGAAGCTGCCGAGCGAACTTTCGCTCTCGCAACAGCTGGGCGTCTCGCGCACGGTCGTTCGCGAGG
CTTTCCGGTCTCTGTCGGCCATGCGGCTGATCGACGTCAACGCGGGCAAGCGCGCAACCGTGGCGACGCTGGACCATGGT
GCGATGTCGCTGATGTTCGAGCATGGCATTCACACCGAGCAGATCAACATCCAGCAGATTTACGACGTACGCCGCACCAT
CGAAGTCAGAACCGTAACGCTCGCAGCGCTCCGGCGCACCGACGCGGAAGCGCTTACCATCCTCGACCACGCCAACAATA
TGGAGCGGGACTTCAGCGACAATGACAGGGTGATGGAGCATGACCTTGCCTTTCACCTTGCTGTTGCCAGAGCCTCCAAG
AACCCGGTTTTCGAACTCATTCTCGGCGCTTTCCAGAATGTGACACGCCAGACCTGGCCGATCGGCTGGAAAAGCCGCAC
CTCCGATGCACAGCGCCTCGCCGCGTGCGAACTGCATATCGCCATCGGACAGGCCATTGCCGCAGGCGACCCGCAAACCG
CCTCAACGCTGATGGCGCGGCATTTCGATGAAAGTGTGCATGCACTTCTGGCAGCCGGCATCGCCTGA

Upstream 100 bases:

>100_bases
CAGATGGAACCGTGCAATCGCGCGGGCCAGGGCGCTTGTCTACGTAGAGAAAATCCTGTAAGCAATTTTCCATAAACCTG
TACTACAGGTTGGAGGAAAA

Downstream 100 bases:

>100_bases
TTTTTTCAACGCAGTCCCAGATGGAAACGACTGCCTCTGGAGGAGACACCATGAAAATCACAAAACTCGAGACCGTCCGT
GTGGCGGAACGGACGAACCT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MLNAISHDSGLVSSTIGAITRHIRENELAPGAKLPSELSLSQQLGVSRTVVREAFRSLSAMRLIDVNAGKRATVATLDHG
AMSLMFEHGIHTEQINIQQIYDVRRTIEVRTVTLAALRRTDAEALTILDHANNMERDFSDNDRVMEHDLAFHLAVARASK
NPVFELILGAFQNVTRQTWPIGWKSRTSDAQRLAACELHIAIGQAIAAGDPQTASTLMARHFDESVHALLAAGIA

Sequences:

>Translated_235_residues
MLNAISHDSGLVSSTIGAITRHIRENELAPGAKLPSELSLSQQLGVSRTVVREAFRSLSAMRLIDVNAGKRATVATLDHG
AMSLMFEHGIHTEQINIQQIYDVRRTIEVRTVTLAALRRTDAEALTILDHANNMERDFSDNDRVMEHDLAFHLAVARASK
NPVFELILGAFQNVTRQTWPIGWKSRTSDAQRLAACELHIAIGQAIAAGDPQTASTLMARHFDESVHALLAAGIA
>Mature_235_residues
MLNAISHDSGLVSSTIGAITRHIRENELAPGAKLPSELSLSQQLGVSRTVVREAFRSLSAMRLIDVNAGKRATVATLDHG
AMSLMFEHGIHTEQINIQQIYDVRRTIEVRTVTLAALRRTDAEALTILDHANNMERDFSDNDRVMEHDLAFHLAVARASK
NPVFELILGAFQNVTRQTWPIGWKSRTSDAQRLAACELHIAIGQAIAAGDPQTASTLMARHFDESVHALLAAGIA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994955, Length=169, Percent_Identity=28.4023668639053, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25719; Mature: 25719

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNAISHDSGLVSSTIGAITRHIRENELAPGAKLPSELSLSQQLGVSRTVVREAFRSLSA
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
MRLIDVNAGKRATVATLDHGAMSLMFEHGIHTEQINIQQIYDVRRTIEVRTVTLAALRRT
EEEEECCCCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
DAEALTILDHANNMERDFSDNDRVMEHDLAFHLAVARASKNPVFELILGAFQNVTRQTWP
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
IGWKSRTSDAQRLAACELHIAIGQAIAAGDPQTASTLMARHFDESVHALLAAGIA
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLNAISHDSGLVSSTIGAITRHIRENELAPGAKLPSELSLSQQLGVSRTVVREAFRSLSA
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
MRLIDVNAGKRATVATLDHGAMSLMFEHGIHTEQINIQQIYDVRRTIEVRTVTLAALRRT
EEEEECCCCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
DAEALTILDHANNMERDFSDNDRVMEHDLAFHLAVARASKNPVFELILGAFQNVTRQTWP
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
IGWKSRTSDAQRLAACELHIAIGQAIAAGDPQTASTLMARHFDESVHALLAAGIA
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]