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The map label for this gene is dctD [H]

Identifier: 159184165

GI number: 159184165

Start: 119383

End: 120729

Strand: Direct

Name: dctD [H]

Synonym: Atu0114

Alternate gene names: 159184165

Gene position: 119383-120729 (Clockwise)

Preceding gene: 159184164

Following gene: 159184166

Centisome position: 4.2

GC content: 60.28

Gene sequence:

>1347_bases
ATGACCATGTCGCGGGTTCTGTTGATCGACGACGAGGAGGAGCTGCGCTTTTCCACCGCCCAGGCACTGGAGCTTTCCGG
TTTTGCCGTCACCATGCTGGCGAGCGCGGAACATGCGCTCGAACTGATCGGCTACAGCTTCGACGGTGTCGTGGTCAGCG
ATATCCGCATGCCCGGCATGGATGGCATGACGCTGCTGCAGAAGGTACGGGAGCTTGACCCGGAAATACCCGTCATCCTG
ATGACGGGCCATGGCGATGTGCAGCTTGCCGTCAACGCCATGCGCAACGGTGCTTATGACTTCATCGAAAAACCCTTCAC
CCCGCAATATCTCGCCGGCATCATCAAGAGGGCAAATGACCGGCGCGCACTGGTGCTGGAAAACCGCCGCCTGAAGGCGG
TTGCCGGCAAGCATGACGATCTCGAAACACGCCTGCCCGGCAGAACCCAGGTCATGGTCGATCTGCGCTACCGCATCCGC
GCCATCGGCGCGACAGACGCCGATACGCTGATCGTGGGTGATACTGGGGCCGGCAAGGAGGTGGTGGCGCGGGCGCTGCA
CGATATCAGCGCCAGAGCCAACCGACCCTTCGTCGCGATCAATTGTGCTGCCCTTCCGCAGACGCTGATCGAGAGCGAAT
TGTTCGGCCATGAGGCCGGTGCTTTTCCGGGCGCACTGCGCTCCCGTTATGGCAAGTTCGAACATGCCCGGGGCGGCACC
ATCCTGCTGGATGAGATTGGCTCCATGCCTTTCGAATTGCAGGGGCGCTTCCTGCGCGTCTTGCAAGAGCGGGTGATTAC
CCGTCTCGGCTCCAATGAGACGGTCGAACTCGATGTCCGCTTCATCGCCACCAGCAAGGTCGATCTGGAGCAGGAAGTTG
CGGCCGGCCGGTTCCGCGCAGACCTTCTTTATCGCCTGAACGTGGCGACGCTTCTTGTGCCTTCGCTTTCGCAGCGGAGT
GCGGACATACCGCTGCTCTTCCTGCATCTGGTACGGGAAGCCGCCGCCCGTTACGGGCGGGAGGATATGGACGTGCCGCA
GCATCTGCTTTCCGCCGTTTCGCTGCGGGAATGGCCGGGCAACGTGCGGGAATTGCGCAATGCCGCGGATCGTTTCGTGC
TCGGTTTGGAAAGCGATGTGTCCGAGGTGTCGCTGGCGCTTGATGATGACGGCACCTCGACGCTGGCGCCGCGGGTTGCC
GCTTATGAAAAAAGCCTGATCGCGCATGCCATTGCAGTGCATGGCGGAAATTTGAAATCCGTCTATGAAAGCCTCGGTAT
TTCACGCAAAACGCTATATGAAAAAATGCAGAAATACGGGCTGCATCGGCATATGACCGAGGTCTGA

Upstream 100 bases:

>100_bases
ATTTCGGCGGTCAGCTGCGGGTGAAGAACCACGAAGAGAGCGGCGCGGAGTTTGAGATAGAATTGCCAGCGGCGGCGTGG
CGCACGGAGGCGGCTGCGGA

Downstream 100 bases:

>100_bases
TGGGTGGATTTCCACCCATGGATGAGGCTCTTTGGGTTGAAATCCACCCATTGCCATCCATTGATAGCGATAAAAGTGGA
TGGCGTGAAGAGAGCGCCGT

Product: two component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTMSRVLLIDDEEELRFSTAQALELSGFAVTMLASAEHALELIGYSFDGVVVSDIRMPGMDGMTLLQKVRELDPEIPVIL
MTGHGDVQLAVNAMRNGAYDFIEKPFTPQYLAGIIKRANDRRALVLENRRLKAVAGKHDDLETRLPGRTQVMVDLRYRIR
AIGATDADTLIVGDTGAGKEVVARALHDISARANRPFVAINCAALPQTLIESELFGHEAGAFPGALRSRYGKFEHARGGT
ILLDEIGSMPFELQGRFLRVLQERVITRLGSNETVELDVRFIATSKVDLEQEVAAGRFRADLLYRLNVATLLVPSLSQRS
ADIPLLFLHLVREAAARYGREDMDVPQHLLSAVSLREWPGNVRELRNAADRFVLGLESDVSEVSLALDDDGTSTLAPRVA
AYEKSLIAHAIAVHGGNLKSVYESLGISRKTLYEKMQKYGLHRHMTEV

Sequences:

>Translated_448_residues
MTMSRVLLIDDEEELRFSTAQALELSGFAVTMLASAEHALELIGYSFDGVVVSDIRMPGMDGMTLLQKVRELDPEIPVIL
MTGHGDVQLAVNAMRNGAYDFIEKPFTPQYLAGIIKRANDRRALVLENRRLKAVAGKHDDLETRLPGRTQVMVDLRYRIR
AIGATDADTLIVGDTGAGKEVVARALHDISARANRPFVAINCAALPQTLIESELFGHEAGAFPGALRSRYGKFEHARGGT
ILLDEIGSMPFELQGRFLRVLQERVITRLGSNETVELDVRFIATSKVDLEQEVAAGRFRADLLYRLNVATLLVPSLSQRS
ADIPLLFLHLVREAAARYGREDMDVPQHLLSAVSLREWPGNVRELRNAADRFVLGLESDVSEVSLALDDDGTSTLAPRVA
AYEKSLIAHAIAVHGGNLKSVYESLGISRKTLYEKMQKYGLHRHMTEV
>Mature_447_residues
TMSRVLLIDDEEELRFSTAQALELSGFAVTMLASAEHALELIGYSFDGVVVSDIRMPGMDGMTLLQKVRELDPEIPVILM
TGHGDVQLAVNAMRNGAYDFIEKPFTPQYLAGIIKRANDRRALVLENRRLKAVAGKHDDLETRLPGRTQVMVDLRYRIRA
IGATDADTLIVGDTGAGKEVVARALHDISARANRPFVAINCAALPQTLIESELFGHEAGAFPGALRSRYGKFEHARGGTI
LLDEIGSMPFELQGRFLRVLQERVITRLGSNETVELDVRFIATSKVDLEQEVAAGRFRADLLYRLNVATLLVPSLSQRSA
DIPLLFLHLVREAAARYGREDMDVPQHLLSAVSLREWPGNVRELRNAADRFVLGLESDVSEVSLALDDDGTSTLAPRVAA
YEKSLIAHAIAVHGGNLKSVYESLGISRKTLYEKMQKYGLHRHMTEV

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=457, Percent_Identity=38.074398249453, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1790437, Length=450, Percent_Identity=39.1111111111111, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=36.5384615384615, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1788905, Length=447, Percent_Identity=36.9127516778523, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1789087, Length=338, Percent_Identity=41.1242603550296, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082117, Length=315, Percent_Identity=41.5873015873016, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=38.2716049382716, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=44.6351931330472, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1786524, Length=313, Percent_Identity=37.3801916932907, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1787583, Length=344, Percent_Identity=32.5581395348837, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87081872, Length=235, Percent_Identity=37.4468085106383, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87081858, Length=291, Percent_Identity=32.3024054982818, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789828, Length=213, Percent_Identity=36.150234741784, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=30.2521008403361, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49525; Mature: 49393

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSRVLLIDDEEELRFSTAQALELSGFAVTMLASAEHALELIGYSFDGVVVSDIRMPGM
CCCCEEEEEECHHHHHHHHHHHHEECCEEEEEHHHHHHHHHHHCCCCCCEEEEEECCCCC
DGMTLLQKVRELDPEIPVILMTGHGDVQLAVNAMRNGAYDFIEKPFTPQYLAGIIKRAND
CCHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHCCCHHHHCCCCCHHHHHHHHHCCCC
RRALVLENRRLKAVAGKHDDLETRLPGRTQVMVDLRYRIRAIGATDADTLIVGDTGAGKE
CEEEEECCCEEEECCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCEEEEECCCCCHH
VVARALHDISARANRPFVAINCAALPQTLIESELFGHEAGAFPGALRSRYGKFEHARGGT
HHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCE
ILLDEIGSMPFELQGRFLRVLQERVITRLGSNETVELDVRFIATSKVDLEQEVAAGRFRA
EEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
DLLYRLNVATLLVPSLSQRSADIPLLFLHLVREAAARYGREDMDVPQHLLSAVSLREWPG
HHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
NVRELRNAADRFVLGLESDVSEVSLALDDDGTSTLAPRVAAYEKSLIAHAIAVHGGNLKS
HHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VYESLGISRKTLYEKMQKYGLHRHMTEV
HHHHHCCCHHHHHHHHHHHCHHHHHHCC
>Mature Secondary Structure 
TMSRVLLIDDEEELRFSTAQALELSGFAVTMLASAEHALELIGYSFDGVVVSDIRMPGM
CCCEEEEEECHHHHHHHHHHHHEECCEEEEEHHHHHHHHHHHCCCCCCEEEEEECCCCC
DGMTLLQKVRELDPEIPVILMTGHGDVQLAVNAMRNGAYDFIEKPFTPQYLAGIIKRAND
CCHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHCCCHHHHCCCCCHHHHHHHHHCCCC
RRALVLENRRLKAVAGKHDDLETRLPGRTQVMVDLRYRIRAIGATDADTLIVGDTGAGKE
CEEEEECCCEEEECCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCEEEEECCCCCHH
VVARALHDISARANRPFVAINCAALPQTLIESELFGHEAGAFPGALRSRYGKFEHARGGT
HHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCE
ILLDEIGSMPFELQGRFLRVLQERVITRLGSNETVELDVRFIATSKVDLEQEVAAGRFRA
EEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
DLLYRLNVATLLVPSLSQRSADIPLLFLHLVREAAARYGREDMDVPQHLLSAVSLREWPG
HHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
NVRELRNAADRFVLGLESDVSEVSLALDDDGTSTLAPRVAAYEKSLIAHAIAVHGGNLKS
HHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VYESLGISRKTLYEKMQKYGLHRHMTEV
HHHHHCCCHHHHHHHHHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]