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The map label for this gene is dctB [H]

Identifier: 159184164

GI number: 159184164

Start: 117518

End: 119386

Strand: Direct

Name: dctB [H]

Synonym: Atu0113

Alternate gene names: 159184164

Gene position: 117518-119386 (Clockwise)

Preceding gene: 159184163

Following gene: 159184165

Centisome position: 4.14

GC content: 59.12

Gene sequence:

>1869_bases
ATGGAGAGCGGGTTTGAGGCCTGCGGCAGGAGGGGAGTTTCGGTGAAATATCGCTGGATTTTGTTGATGTTTCTCGGCCT
AGGTCTCGGTTATGGCGCCGTGACGAGAGGTGGCGGCATCATCGCGGAAAGCTATTTCGGCGAAATGTCCGATCAGGGCC
ACACGACGCTCCGGCTAGCCGTCTCGGCACTTGGCGGGCTCCTTAGCCGCTATGAGCCACTTCCCGCGCTAATCGCCGAT
CATGACGACATCGAGGAACTGGTCGCGCATCCCAAGGATGAAGCCCTGCGCCAGCGCGCCAATGTCTACCTCAAATCCAT
CAACACCCTGCTGGAATCCTCCGATATCTATATCATCACGCTGGATGGGGAGACGATCGCGGCCAGCAATTACGATGGGC
CAACGAGTTTCGTCGGTGAGAATTTCAGTTACCGGCCCTATTTTCAGGATGCCGCCAAGGGCCTCAAGTCCCGCTTTTTT
GCTCTCGGCACCACCTCCCACAAGCGCGGTTATTATTTCTCCGCGCCGATTCTCTTTAATGAAGAAATCAAGGGCGTCAT
TGTCTTCAAGGTGGATATTGAAGGCATAGAGGCATCCTTCGGGGATGGCGAAAACCGCATTCTGGTTTCGGACCCGGAAG
GCATTATCTTCATGACCGGCACGCCGCAATGGCTTTATTCCGGCCTGATGCCACTGACGCCGGAGAGGCTGGCGCGCACC
GAAGCCTCCCGTCGTTATGCTAATGCGACCCTCACGGAACTGCCGCTTAAAAACGGCAATTTCGGCTCGCACCAGCTGAT
GACGATCGCCCAGAATGATGGTGACAGGGAATATATGGTCCTGTCGCAGCCGATGCAGGATGCCGGCTGGACCGTCAGCG
TGCTGATGGATACGGGTTCGCTCCGCACGCAGGTCAAGACCGCGATGATTGCCATCATCCTCTGCCTGTGCCTCGCTGCG
GCGCTGATTGCCGCCATGCTGCAGAGACGGCGGCGTCTGCGCGAGCGCCTGACCCACCAGGCGGAAGCGCAGGCGGAGCT
GGAGCGCCGGGTGGAGGAGCGCACGGCCGATCTGGCACGGGTCAATCAGGAAATCAAACACGAGATCGCCGAGCGACGCC
AGACCGAAAAGCAGCTGCGCAAGATGCAGAATGATCTGGTGCAGGCGGGCAAGCTGGCGGCACTTGGGCAGATGTCGGCG
GCGCTGTCTCACGAATTTAACCAGCCGCTGGCAGCCGCCAAGAACTATGCCGAAAACGCCTCGCTTCTGGTGGAGCGCGG
GCGTCTGGAAGAGGTGACGGAAAATCTCAGGCGTATTTCCGGCCTGATCGACCGTATGGCGTCGATCAGCAAACATCTCA
GGAACTTTGCCCGCAAGCCCAACGAGAAAACGGCAGCGGTGGGTCTGGAAACCGTAATTCGCGATACGCTCGAAATTGTG
GGCCCGCGGCTGAAAGCCGCCGATGCAATGCTGGATATTGATCTTGGTCCGGTGCCGCTTGCCGTGAAAGCTGGTCCGGT
GCGCCTGCAGCAGGTCCTCGTCAACATCATCTCCAATGCTGCCGATGCCGTGGAAGGGCGCGAAGATCGCCGCATTTCGC
TACAGACCGTGCAGCACGGGCAGGCGGTATCGATTTTCATCCGCGACCATGGCCCCGGCGTACCGGCGGCGATTTCGGAA
CGCATCTTCGATCCGTTTTTCACTACCAAGGGTGTCGGTCGTGGCCTCGGTCTCGGCCTTTCCATCTCCTATAACATTAT
CAAGGATTTCGGCGGTCAGCTGCGGGTGAAGAACCACGAAGAGAGCGGCGCGGAGTTTGAGATAGAATTGCCAGCGGCGG
CGTGGCGCACGGAGGCGGCTGCGGAATGA

Upstream 100 bases:

>100_bases
GCAAAAAACCGGTCCTCGTGGCCGGTTTTTTGTTTTCAGGACTGTCCGGCTGCCTTTTCCGCTCACTTGGAAAATTCCGC
CACTCCTCCTACACTCTTTC

Downstream 100 bases:

>100_bases
CCATGTCGCGGGTTCTGTTGATCGACGACGAGGAGGAGCTGCGCTTTTCCACCGCCCAGGCACTGGAGCTTTCCGGTTTT
GCCGTCACCATGCTGGCGAG

Product: two component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MESGFEACGRRGVSVKYRWILLMFLGLGLGYGAVTRGGGIIAESYFGEMSDQGHTTLRLAVSALGGLLSRYEPLPALIAD
HDDIEELVAHPKDEALRQRANVYLKSINTLLESSDIYIITLDGETIAASNYDGPTSFVGENFSYRPYFQDAAKGLKSRFF
ALGTTSHKRGYYFSAPILFNEEIKGVIVFKVDIEGIEASFGDGENRILVSDPEGIIFMTGTPQWLYSGLMPLTPERLART
EASRRYANATLTELPLKNGNFGSHQLMTIAQNDGDREYMVLSQPMQDAGWTVSVLMDTGSLRTQVKTAMIAIILCLCLAA
ALIAAMLQRRRRLRERLTHQAEAQAELERRVEERTADLARVNQEIKHEIAERRQTEKQLRKMQNDLVQAGKLAALGQMSA
ALSHEFNQPLAAAKNYAENASLLVERGRLEEVTENLRRISGLIDRMASISKHLRNFARKPNEKTAAVGLETVIRDTLEIV
GPRLKAADAMLDIDLGPVPLAVKAGPVRLQQVLVNIISNAADAVEGREDRRISLQTVQHGQAVSIFIRDHGPGVPAAISE
RIFDPFFTTKGVGRGLGLGLSISYNIIKDFGGQLRVKNHEESGAEFEIELPAAAWRTEAAAE

Sequences:

>Translated_622_residues
MESGFEACGRRGVSVKYRWILLMFLGLGLGYGAVTRGGGIIAESYFGEMSDQGHTTLRLAVSALGGLLSRYEPLPALIAD
HDDIEELVAHPKDEALRQRANVYLKSINTLLESSDIYIITLDGETIAASNYDGPTSFVGENFSYRPYFQDAAKGLKSRFF
ALGTTSHKRGYYFSAPILFNEEIKGVIVFKVDIEGIEASFGDGENRILVSDPEGIIFMTGTPQWLYSGLMPLTPERLART
EASRRYANATLTELPLKNGNFGSHQLMTIAQNDGDREYMVLSQPMQDAGWTVSVLMDTGSLRTQVKTAMIAIILCLCLAA
ALIAAMLQRRRRLRERLTHQAEAQAELERRVEERTADLARVNQEIKHEIAERRQTEKQLRKMQNDLVQAGKLAALGQMSA
ALSHEFNQPLAAAKNYAENASLLVERGRLEEVTENLRRISGLIDRMASISKHLRNFARKPNEKTAAVGLETVIRDTLEIV
GPRLKAADAMLDIDLGPVPLAVKAGPVRLQQVLVNIISNAADAVEGREDRRISLQTVQHGQAVSIFIRDHGPGVPAAISE
RIFDPFFTTKGVGRGLGLGLSISYNIIKDFGGQLRVKNHEESGAEFEIELPAAAWRTEAAAE
>Mature_622_residues
MESGFEACGRRGVSVKYRWILLMFLGLGLGYGAVTRGGGIIAESYFGEMSDQGHTTLRLAVSALGGLLSRYEPLPALIAD
HDDIEELVAHPKDEALRQRANVYLKSINTLLESSDIYIITLDGETIAASNYDGPTSFVGENFSYRPYFQDAAKGLKSRFF
ALGTTSHKRGYYFSAPILFNEEIKGVIVFKVDIEGIEASFGDGENRILVSDPEGIIFMTGTPQWLYSGLMPLTPERLART
EASRRYANATLTELPLKNGNFGSHQLMTIAQNDGDREYMVLSQPMQDAGWTVSVLMDTGSLRTQVKTAMIAIILCLCLAA
ALIAAMLQRRRRLRERLTHQAEAQAELERRVEERTADLARVNQEIKHEIAERRQTEKQLRKMQNDLVQAGKLAALGQMSA
ALSHEFNQPLAAAKNYAENASLLVERGRLEEVTENLRRISGLIDRMASISKHLRNFARKPNEKTAAVGLETVIRDTLEIV
GPRLKAADAMLDIDLGPVPLAVKAGPVRLQQVLVNIISNAADAVEGREDRRISLQTVQHGQAVSIFIRDHGPGVPAAISE
RIFDPFFTTKGVGRGLGLGLSISYNIIKDFGGQLRVKNHEESGAEFEIELPAAAWRTEAAAE

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=234, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788549, Length=239, Percent_Identity=33.4728033472803, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI145693157, Length=259, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081816, Length=276, Percent_Identity=27.1739130434783, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI87082128, Length=223, Percent_Identity=30.4932735426009, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1786912, Length=225, Percent_Identity=28.4444444444444, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790300, Length=245, Percent_Identity=26.9387755102041, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 68552; Mature: 68552

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESGFEACGRRGVSVKYRWILLMFLGLGLGYGAVTRGGGIIAESYFGEMSDQGHTTLRLA
CCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCEEEHHHHCCCCCCCCHHHHHH
VSALGGLLSRYEPLPALIADHDDIEELVAHPKDEALRQRANVYLKSINTLLESSDIYIIT
HHHHHHHHHHCCCCCHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LDGETIAASNYDGPTSFVGENFSYRPYFQDAAKGLKSRFFALGTTSHKRGYYFSAPILFN
ECCCEEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEECCEEEC
EEIKGVIVFKVDIEGIEASFGDGENRILVSDPEGIIFMTGTPQWLYSGLMPLTPERLART
CCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCEEEEECCHHHHHCCCCCCCHHHHHHH
EASRRYANATLTELPLKNGNFGSHQLMTIAQNDGDREYMVLSQPMQDAGWTVSVLMDTGS
HHHHHHHCCCHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCEEEEEEECCC
LRTQVKTAMIAIILCLCLAAALIAAMLQRRRRLRERLTHQAEAQAELERRVEERTADLAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNQEIKHEIAERRQTEKQLRKMQNDLVQAGKLAALGQMSAALSHEFNQPLAAAKNYAENA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCH
SLLVERGRLEEVTENLRRISGLIDRMASISKHLRNFARKPNEKTAAVGLETVIRDTLEIV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GPRLKAADAMLDIDLGPVPLAVKAGPVRLQQVLVNIISNAADAVEGREDRRISLQTVQHG
CCCHHHHHHEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
QAVSIFIRDHGPGVPAAISERIFDPFFTTKGVGRGLGLGLSISYNIIKDFGGQLRVKNHE
CEEEEEEEECCCCCCHHHHHHHHCHHHHHCCCCCCCCCCEEEEHHHHHHCCCCEEEECCC
ESGAEFEIELPAAAWRTEAAAE
CCCCEEEEECCHHHHHHHCCCC
>Mature Secondary Structure
MESGFEACGRRGVSVKYRWILLMFLGLGLGYGAVTRGGGIIAESYFGEMSDQGHTTLRLA
CCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCEEEHHHHCCCCCCCCHHHHHH
VSALGGLLSRYEPLPALIADHDDIEELVAHPKDEALRQRANVYLKSINTLLESSDIYIIT
HHHHHHHHHHCCCCCHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LDGETIAASNYDGPTSFVGENFSYRPYFQDAAKGLKSRFFALGTTSHKRGYYFSAPILFN
ECCCEEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEECCEEEC
EEIKGVIVFKVDIEGIEASFGDGENRILVSDPEGIIFMTGTPQWLYSGLMPLTPERLART
CCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCEEEEECCHHHHHCCCCCCCHHHHHHH
EASRRYANATLTELPLKNGNFGSHQLMTIAQNDGDREYMVLSQPMQDAGWTVSVLMDTGS
HHHHHHHCCCHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCEEEEEEECCC
LRTQVKTAMIAIILCLCLAAALIAAMLQRRRRLRERLTHQAEAQAELERRVEERTADLAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNQEIKHEIAERRQTEKQLRKMQNDLVQAGKLAALGQMSAALSHEFNQPLAAAKNYAENA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCH
SLLVERGRLEEVTENLRRISGLIDRMASISKHLRNFARKPNEKTAAVGLETVIRDTLEIV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GPRLKAADAMLDIDLGPVPLAVKAGPVRLQQVLVNIISNAADAVEGREDRRISLQTVQHG
CCCHHHHHHEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
QAVSIFIRDHGPGVPAAISERIFDPFFTTKGVGRGLGLGLSISYNIIKDFGGQLRVKNHE
CEEEEEEEECCCCCCHHHHHHHHCHHHHHCCCCCCCCCCEEEEHHHHHHCCCCEEEECCC
ESGAEFEIELPAAAWRTEAAAE
CCCCEEEEECCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3671068 [H]