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The map label for this gene is dapE [H]

Identifier: 159184160

GI number: 159184160

Start: 110444

End: 111835

Strand: Reverse

Name: dapE [H]

Synonym: Atu0108

Alternate gene names: 159184160

Gene position: 111835-110444 (Counterclockwise)

Preceding gene: 159184161

Following gene: 159184157

Centisome position: 3.94

GC content: 60.85

Gene sequence:

>1392_bases
ATGACAGACGTTTCCCCTATTCTTTCCAACGCCGACGACAATCTGACTTCCAGCCTGGAGCGGCTTTTCGAACTCGTGCG
CATTCCGTCGATTTCCACCGATCCCGCCTATAAGGCAGAATGCCGCAAGGCTGCGGACTGGCTGGTGAGGACGCTTTCCG
CGCTCGGTTTTACGGCTTCGGTGCGCGATACGGCCGGTCACCCGATGGTGGTTGCGCATCACGATGCGGCGACCAAGGAT
GCGCCGCATGTACTTTTTTACGGCCATTATGACGTTCAGCCGGTCGATCCGCTGGAACTCTGGGAAAATGATCCGTTCGA
GCCTGCCATCAAGGATATCGGCGCCGGCCGGAAGGTGATCACCGGACGTGGCACGGCTGACGACAAGGGCCAGCTGATGA
CCTTCGTCGAGGCCTGCCGGGCCTATAAGGCAGTCAATGGTGTCCTGCCAGTGCGTGTTACCATTCTGTTTGAAGGCGAA
GAGGAATCCGGCTCGCCTTCGCTGAAACCGTTCCTCGACGCCAACGCGCAGGAACTGAAGGCCGATTATGCGCTGGTCTG
CGATACCGGCATGTGGGACCGGGATACGCCCGCCATCGCCGCTGCGCTTCGCGGGCTGGTTGGCGAAGAGATCGTCATCA
CCGCGGCAGACCGCGACCTGCATTCCGGCCTTTTTGGCGGCGCCGCCGCAAATCCGATCCACATCCTCACCGATATCCTC
GCCGGACTGCATGACGAGACGGGCCGCGTGACGCTTGCCGGCTTTTATGACGGTGTGGAAGAACCCCCTGCCAACATCAA
GGCATCCTGGGAAACGCTCGGACGTTCGGCGGAAGCCTTCCTCGGTGAAGTCGGCCTTTCGGTTCCCTCGGGTGAAAAGG
GCCGTTCCGTGCTGGAGCAGACCTGGGCGCGGCCGACGGCGGAAGTCAACGGCATCATCGGCGGCTACACCGGCGATGGT
TTCAAGACCGTGATTGCGGCGAAAGCCTCCGCCAAGGTGTCGTTCCGCCTCGTCGGCGAGCAGAACCCCGCAGCCATTCG
CGACAGCTTCCGGGCCTATGTGCGCTCCAAAATTCCAGCCGATTGCTCCGTGGAGTTCCACGAGCATGGCGCATCACCAG
CCATCCAGCTGTCTTACGACTCGCCCGTGCTCACCAAGGCCAAAAACGCGCTATCGGAAGAGTGGCCAAAACCCGCCGTT
GTCATCGGCATGGGCGGCTCGATCCCGATCGTCGGCGATTTCCAGAAGATGCTGGGCATGGATTCGCTGCTCGTCGGCTT
CGGCCTGACTGACGACCGCATTCATTCGCCGAATGAAAAATACGAGCTGCAATCCTTCCATAAGGGCATCCGCTCCTGGG
TCCGCATTCTGGACGCACTCGCAGCGAAATGA

Upstream 100 bases:

>100_bases
CTTGCCGATTCACGACAAGCCCGCGCCTTGTTTCCCGCGATGTGCGATGCCCGGATATTTCGTCTTTCGCCGGGTCACGA
CAGACATGACAGGATCAGCG

Downstream 100 bases:

>100_bases
CAAAAAAAAGAGGAAGCACCGGGTAATCCGGCGCTTCCTCTTTTCCGTTTGCGCATCGCAAAAACAAAAAGGCCGGGCGA
GCCCGACCTTTCCATCCGTT

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTDVSPILSNADDNLTSSLERLFELVRIPSISTDPAYKAECRKAADWLVRTLSALGFTASVRDTAGHPMVVAHHDAATKD
APHVLFYGHYDVQPVDPLELWENDPFEPAIKDIGAGRKVITGRGTADDKGQLMTFVEACRAYKAVNGVLPVRVTILFEGE
EESGSPSLKPFLDANAQELKADYALVCDTGMWDRDTPAIAAALRGLVGEEIVITAADRDLHSGLFGGAAANPIHILTDIL
AGLHDETGRVTLAGFYDGVEEPPANIKASWETLGRSAEAFLGEVGLSVPSGEKGRSVLEQTWARPTAEVNGIIGGYTGDG
FKTVIAAKASAKVSFRLVGEQNPAAIRDSFRAYVRSKIPADCSVEFHEHGASPAIQLSYDSPVLTKAKNALSEEWPKPAV
VIGMGGSIPIVGDFQKMLGMDSLLVGFGLTDDRIHSPNEKYELQSFHKGIRSWVRILDALAAK

Sequences:

>Translated_463_residues
MTDVSPILSNADDNLTSSLERLFELVRIPSISTDPAYKAECRKAADWLVRTLSALGFTASVRDTAGHPMVVAHHDAATKD
APHVLFYGHYDVQPVDPLELWENDPFEPAIKDIGAGRKVITGRGTADDKGQLMTFVEACRAYKAVNGVLPVRVTILFEGE
EESGSPSLKPFLDANAQELKADYALVCDTGMWDRDTPAIAAALRGLVGEEIVITAADRDLHSGLFGGAAANPIHILTDIL
AGLHDETGRVTLAGFYDGVEEPPANIKASWETLGRSAEAFLGEVGLSVPSGEKGRSVLEQTWARPTAEVNGIIGGYTGDG
FKTVIAAKASAKVSFRLVGEQNPAAIRDSFRAYVRSKIPADCSVEFHEHGASPAIQLSYDSPVLTKAKNALSEEWPKPAV
VIGMGGSIPIVGDFQKMLGMDSLLVGFGLTDDRIHSPNEKYELQSFHKGIRSWVRILDALAAK
>Mature_462_residues
TDVSPILSNADDNLTSSLERLFELVRIPSISTDPAYKAECRKAADWLVRTLSALGFTASVRDTAGHPMVVAHHDAATKDA
PHVLFYGHYDVQPVDPLELWENDPFEPAIKDIGAGRKVITGRGTADDKGQLMTFVEACRAYKAVNGVLPVRVTILFEGEE
ESGSPSLKPFLDANAQELKADYALVCDTGMWDRDTPAIAAALRGLVGEEIVITAADRDLHSGLFGGAAANPIHILTDILA
GLHDETGRVTLAGFYDGVEEPPANIKASWETLGRSAEAFLGEVGLSVPSGEKGRSVLEQTWARPTAEVNGIIGGYTGDGF
KTVIAAKASAKVSFRLVGEQNPAAIRDSFRAYVRSKIPADCSVEFHEHGASPAIQLSYDSPVLTKAKNALSEEWPKPAVV
IGMGGSIPIVGDFQKMLGMDSLLVGFGLTDDRIHSPNEKYELQSFHKGIRSWVRILDALAAK

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=468, Percent_Identity=26.0683760683761, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI21071039, Length=374, Percent_Identity=27.5401069518717, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI271398277, Length=310, Percent_Identity=27.0967741935484, Blast_Score=126, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17563146, Length=467, Percent_Identity=29.1220556745182, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI193211059, Length=478, Percent_Identity=26.1506276150628, Blast_Score=166, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321159, Length=459, Percent_Identity=27.8867102396514, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6319758, Length=460, Percent_Identity=22.8260869565217, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585880, Length=455, Percent_Identity=29.010989010989, Blast_Score=175, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 49747; Mature: 49616

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVSPILSNADDNLTSSLERLFELVRIPSISTDPAYKAECRKAADWLVRTLSALGFTAS
CCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEE
VRDTAGHPMVVAHHDAATKDAPHVLFYGHYDVQPVDPLELWENDPFEPAIKDIGAGRKVI
CCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCHHHHHCCCCCEEE
TGRGTADDKGQLMTFVEACRAYKAVNGVLPVRVTILFEGEEESGSPSLKPFLDANAQELK
ECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHCCCHHHHC
ADYALVCDTGMWDRDTPAIAAALRGLVGEEIVITAADRDLHSGLFGGAAANPIHILTDIL
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCHHHHHHHHH
AGLHDETGRVTLAGFYDGVEEPPANIKASWETLGRSAEAFLGEVGLSVPSGEKGRSVLEQ
HHCCCCCCCEEEEEECCCCCCCCCCCEECHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHH
TWARPTAEVNGIIGGYTGDGFKTVIAAKASAKVSFRLVGEQNPAAIRDSFRAYVRSKIPA
HHCCCCCHHCCEEECCCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCC
DCSVEFHEHGASPAIQLSYDSPVLTKAKNALSEEWPKPAVVIGMGGSIPIVGDFQKMLGM
CCEEEHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEECHHHHHCC
DSLLVGFGLTDDRIHSPNEKYELQSFHKGIRSWVRILDALAAK
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDVSPILSNADDNLTSSLERLFELVRIPSISTDPAYKAECRKAADWLVRTLSALGFTAS
CCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEE
VRDTAGHPMVVAHHDAATKDAPHVLFYGHYDVQPVDPLELWENDPFEPAIKDIGAGRKVI
CCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCHHHHHCCCCCEEE
TGRGTADDKGQLMTFVEACRAYKAVNGVLPVRVTILFEGEEESGSPSLKPFLDANAQELK
ECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHCCCHHHHC
ADYALVCDTGMWDRDTPAIAAALRGLVGEEIVITAADRDLHSGLFGGAAANPIHILTDIL
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCHHHHHHHHH
AGLHDETGRVTLAGFYDGVEEPPANIKASWETLGRSAEAFLGEVGLSVPSGEKGRSVLEQ
HHCCCCCCCEEEEEECCCCCCCCCCCEECHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHH
TWARPTAEVNGIIGGYTGDGFKTVIAAKASAKVSFRLVGEQNPAAIRDSFRAYVRSKIPA
HHCCCCCHHCCEEECCCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCC
DCSVEFHEHGASPAIQLSYDSPVLTKAKNALSEEWPKPAVVIGMGGSIPIVGDFQKMLGM
CCEEEHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEECHHHHHCC
DSLLVGFGLTDDRIHSPNEKYELQSFHKGIRSWVRILDALAAK
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA