Click here to switch to the map view.

The map label for this gene is gdhA-3 [H]

Identifier: 15898731

GI number: 15898731

Start: 1749834

End: 1751138

Strand: Reverse

Name: gdhA-3 [H]

Synonym: SSO1930

Alternate gene names: 15898731

Gene position: 1751138-1749834 (Counterclockwise)

Preceding gene: 15898734

Following gene: 15898728

Centisome position: 58.52

GC content: 38.16

Gene sequence:

>1305_bases
TTGAGTTGGAATAATTTTTTATTCTTAAAGTATTTGATTAAAGCGATGGAAGAACTCCTTACCTCGAATTTGTTCGTCCA
ACAAGTTAAGAAGTTGTATAAAGTTGGAGAATTGTTGGGTTTAGATATTGATACTCTAGAAGCCCTTTCCCAGCCGGAAA
GGGTTATACAAGTTAAGATACAAATTAGGGGTTCTGATGGTAAGTTAAGGACGTTTGTGGGTTGGAGAAGTCAACACAAT
TCGGCCTTAGGCCCATATAAGGGAGGAGTTAGATACAGTCCCAATGTTACACAAGAAGAAGTAATAGCATTATCAATGAT
TATGACGTGGAAGAATTCCTTGTTATTATTACCCTATGGTGGAGGAAAGGGTGGGATAAGAGTAGATCCTAAGAAGCTAA
CATTAAAGGAATTGGAGGATCTGTCTAGAAAATACATTCAATTAATTCACAACTATTTGGGTAGTAATGTGGACATCCCA
GCGCCTGACATTAATACTAATCCTCAAACTATGGCTTGGTTCCTAGACGAATACATAAAGATAACTGGTGAAGTTGATTT
CGCAGTGTTTACCGGTAAGCCTTATGAACTAGGAGGGATAGGTGTTAGATTATACAGTACAGGGTTAGGTGTAGCAACAA
TTGCTAGAGAGGCTGCTAATAAGTTCATAGGAGGAATTGAAGGATCAAGGGTTATAATTCAAGGGTTCGGAAACGTTGGT
TCTTTTACCGCTAAATTCTTGAGCGAAATGGGGGCTAAAATAATTGGAGTTAGTGATATTGGGGGAGGGGTAATTAATGA
GAATGGAATAGACGTTAATAAGGCGTTGGAAGTTGTTCAGAGAACGGGTAGTGTTGTGAATTATCCAGAGGGTAAAAAGG
TTACAAATGAAGAGTTGCTAATAAGTGATTGTGATATATTAATTCCAGCCGCAGTGGAAAATGTCATAAATAAATTTAAT
GCACCCAAGGTTAAGGCTAAGCTTATTGTTGAAGGTGCAAATGGTCCATTGACTGCAGATGCAGACGATGTAATAAAACA
AAGTGGTATAGTTGTTATACCAGACATTTTGGCTAATGCTGGTGGAGTCGTTGGAAGTTACGTGGAGTGGGCTAACAATA
AGTCTGGTGGGATAATTAGTGATGAGGAGGCTAAGAAACTTATTATTGATAGAATGACTAACGCTTTTAACACTTTATAC
GATTTTCATAAGAGGAAGTTTGCTGATCAAGATTTGAGGACTGTGGCAATGGCTTTAGCTGTGGATAGAGTAGTAAAGGC
AATGAAGGCCAGAGGTCTATTATAA

Upstream 100 bases:

>100_bases
CGCTAAAAACCTTATATTTAGTTTCTGCACTTTGAGAAGACCTCTAATATCAATTTGTCTTTCTCACTTTAGGAGAACTG
TATATTAAAAATTTTCAAAA

Downstream 100 bases:

>100_bases
TCAAGAACTATATTCTTCTTTTTTCCTCTCTTCTAAATTTGTAAGGAAAATTTAGTCTCTGCGAGTGTTAAACTACATCA
GAGTAGATGCATATTTTATT

Product: NAD specific glutamate dehydrogenase (gdhA-3)

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSWNNFLFLKYLIKAMEELLTSNLFVQQVKKLYKVGELLGLDIDTLEALSQPERVIQVKIQIRGSDGKLRTFVGWRSQHN
SALGPYKGGVRYSPNVTQEEVIALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTLKELEDLSRKYIQLIHNYLGSNVDIP
APDINTNPQTMAWFLDEYIKITGEVDFAVFTGKPYELGGIGVRLYSTGLGVATIAREAANKFIGGIEGSRVIIQGFGNVG
SFTAKFLSEMGAKIIGVSDIGGGVINENGIDVNKALEVVQRTGSVVNYPEGKKVTNEELLISDCDILIPAAVENVINKFN
APKVKAKLIVEGANGPLTADADDVIKQSGIVVIPDILANAGGVVGSYVEWANNKSGGIISDEEAKKLIIDRMTNAFNTLY
DFHKRKFADQDLRTVAMALAVDRVVKAMKARGLL

Sequences:

>Translated_434_residues
MSWNNFLFLKYLIKAMEELLTSNLFVQQVKKLYKVGELLGLDIDTLEALSQPERVIQVKIQIRGSDGKLRTFVGWRSQHN
SALGPYKGGVRYSPNVTQEEVIALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTLKELEDLSRKYIQLIHNYLGSNVDIP
APDINTNPQTMAWFLDEYIKITGEVDFAVFTGKPYELGGIGVRLYSTGLGVATIAREAANKFIGGIEGSRVIIQGFGNVG
SFTAKFLSEMGAKIIGVSDIGGGVINENGIDVNKALEVVQRTGSVVNYPEGKKVTNEELLISDCDILIPAAVENVINKFN
APKVKAKLIVEGANGPLTADADDVIKQSGIVVIPDILANAGGVVGSYVEWANNKSGGIISDEEAKKLIIDRMTNAFNTLY
DFHKRKFADQDLRTVAMALAVDRVVKAMKARGLL
>Mature_433_residues
SWNNFLFLKYLIKAMEELLTSNLFVQQVKKLYKVGELLGLDIDTLEALSQPERVIQVKIQIRGSDGKLRTFVGWRSQHNS
ALGPYKGGVRYSPNVTQEEVIALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTLKELEDLSRKYIQLIHNYLGSNVDIPA
PDINTNPQTMAWFLDEYIKITGEVDFAVFTGKPYELGGIGVRLYSTGLGVATIAREAANKFIGGIEGSRVIIQGFGNVGS
FTAKFLSEMGAKIIGVSDIGGGVINENGIDVNKALEVVQRTGSVVNYPEGKKVTNEELLISDCDILIPAAVENVINKFNA
PKVKAKLIVEGANGPLTADADDVIKQSGIVVIPDILANAGGVVGSYVEWANNKSGGIISDEEAKKLIIDRMTNAFNTLYD
FHKRKFADQDLRTVAMALAVDRVVKAMKARGLL

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=333, Percent_Identity=40.2402402402402, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI4885281, Length=333, Percent_Identity=39.9399399399399, Blast_Score=249, Evalue=3e-66,
Organism=Escherichia coli, GI1788059, Length=343, Percent_Identity=32.6530612244898, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17544676, Length=397, Percent_Identity=37.5314861460957, Blast_Score=245, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6324951, Length=396, Percent_Identity=28.7878787878788, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319256, Length=394, Percent_Identity=28.6802030456853, Blast_Score=126, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24649283, Length=362, Percent_Identity=39.5027624309392, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI45549226, Length=339, Percent_Identity=39.5280235988201, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI45553475, Length=339, Percent_Identity=39.5280235988201, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=40.1433691756272, Blast_Score=198, Evalue=6e-51,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 47351; Mature: 47220

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWNNFLFLKYLIKAMEELLTSNLFVQQVKKLYKVGELLGLDIDTLEALSQPERVIQVKI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEE
QIRGSDGKLRTFVGWRSQHNSALGPYKGGVRYSPNVTQEEVIALSMIMTWKNSLLLLPYG
EEECCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEEEEEC
GGKGGIRVDPKKLTLKELEDLSRKYIQLIHNYLGSNVDIPAPDINTNPQTMAWFLDEYIK
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
ITGEVDFAVFTGKPYELGGIGVRLYSTGLGVATIAREAANKFIGGIEGSRVIIQGFGNVG
HCCCEEEEEEECCCEEECCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCH
SFTAKFLSEMGAKIIGVSDIGGGVINENGIDVNKALEVVQRTGSVVNYPEGKKVTNEELL
HHHHHHHHHCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCEE
ISDCDILIPAAVENVINKFNAPKVKAKLIVEGANGPLTADADDVIKQSGIVVIPDILANA
EECCCEEEHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCEEECHHHCCC
GGVVGSYVEWANNKSGGIISDEEAKKLIIDRMTNAFNTLYDFHKRKFADQDLRTVAMALA
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
VDRVVKAMKARGLL
HHHHHHHHHHCCCC
>Mature Secondary Structure 
SWNNFLFLKYLIKAMEELLTSNLFVQQVKKLYKVGELLGLDIDTLEALSQPERVIQVKI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEE
QIRGSDGKLRTFVGWRSQHNSALGPYKGGVRYSPNVTQEEVIALSMIMTWKNSLLLLPYG
EEECCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEEEEEC
GGKGGIRVDPKKLTLKELEDLSRKYIQLIHNYLGSNVDIPAPDINTNPQTMAWFLDEYIK
CCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
ITGEVDFAVFTGKPYELGGIGVRLYSTGLGVATIAREAANKFIGGIEGSRVIIQGFGNVG
HCCCEEEEEEECCCEEECCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCH
SFTAKFLSEMGAKIIGVSDIGGGVINENGIDVNKALEVVQRTGSVVNYPEGKKVTNEELL
HHHHHHHHHCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCEE
ISDCDILIPAAVENVINKFNAPKVKAKLIVEGANGPLTADADDVIKQSGIVVIPDILANA
EECCCEEEHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCEEECHHHCCC
GGVVGSYVEWANNKSGGIISDEEAKKLIIDRMTNAFNTLYDFHKRKFADQDLRTVAMALA
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
VDRVVKAMKARGLL
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8125334 [H]