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The map label for this gene is huyB-1

Identifier: 15898479

GI number: 15898479

Start: 1515235

End: 1516986

Strand: Reverse

Name: huyB-1

Synonym: SSO1662

Alternate gene names: NA

Gene position: 1516986-1515235 (Counterclockwise)

Preceding gene: 15898480

Following gene: 15898472

Centisome position: 50.7

GC content: 39.9

Gene sequence:

>1752_bases
ATGGTAGACCCATTTACATTGGAAATTATAAAGAATGGCCTTGTAGTGGCGTCTGAGGAAATGTTTTACGCTTTCGGAAG
AACCGCTAAAAGTCCGGTTATATATGAGGTACTGGATTACGCTGTTGGAATAACTGATTCTAAGGGGAGAGGTTTGGTGG
CGCAAGCACCAGGAGTACCAGGGTTCTCTGGGGTTTTAGACTTTACGATAAGAGAGGTATTAGGAAAGTGGAAAAGCGAT
ATAAATCCCGGTGATGTGATAGCTACTAATATTCCATATTCTGGAGGTACGCATCTCAACGACGTCGCATTAATTATGCC
TATGTTCACTAAGGATACGTTAGTCGGGTTCATAGTAAATAAAGGGCATTGGAGTGAAATTGGGGGAATGCACTTCGGCA
GTTGGAGTTCAGATGCCACGGAAATATTCCAAGAGGGGCTTCAATTACCAAACATTAAATTATACATTGGAGGAAAACCC
AATAGAGACGTAATTGATATAATATCTGCTAATTCCAGAATGCCTAATGCTACATTAGGGGACATGGAAGCCCAGATCGC
TTCTATGAAAATTGCAGCAAGGAGAATAAACACACTTATTGAGAAATATGGCTTAGATAACGTAATTGAAGCTATGGATA
AGCTCATAGAAGATGGGGTCAAATTAAGCAAGTTGAAACTAGCGAAATTACCTAAAGGTAAATTTGAAGCGGAAGACTAT
ATTGACGATGATGGAATTTCCGACAACCCCGTTTATGTCAAGGTAAGGGTTTCCATAACTGATAACGAATTCATTGTCGA
CTTCACCGGAAGCTCTAATCAAGTCAATGGGCCTATAAATTCTCCTTTACCTGCAACTGTTTCTGCAGTTAGAGAGACGT
ACATGGCTATAACGGATCCTCATGCTTTACCTAATGCAGGATTCTTTTCACCTCTAAAAGTGATAGCACCGGAGGGAAGT
ATATTCAATCCCAGAAAACCAGCTCCGACGTCAACATACTGGGAGTCAATGTCATACGCAACAGATTTAGTATGGAAAGC
ACTAGCTCCCTATATACCAGATAAATTATCTGCTGGACACTTTTTATCAATATTAGCTACAATCTTAGGAGGATATGATG
AAAAGACTGGTGAACCCTTTGCAATCGTAGAACCTCAACCAGGCGGTTGGGGAGGATGTAATGATCAAGATGGGGAAAGC
GCACTAGTAGCTAGCGGTGATGGAGAAACATATATAGCGTCTGCAGAGGTTTATGAAAGAAAAATGCCCATTTTAGTTGA
AAGAGAAAGTCTAAATACAGAAGACGGTCCCGGTCACGGAAAATATAGGGGAGGTCTGGGAATTATTAAGGATTACGTAG
TATTATCTAAAGAGGCATACTTTACAGTCTCAATAGGAAGATCCAAATTTCCACCCTGGGGAATTAATGGAGGATTTAGT
GGTCCTCCAAACTATGCAGTGATTTATAAGAAAGGAGAAAAACCCAGAGTTGCCAGAAAGATAGCAGCGATAAAATTAGA
GTATGGGGACAAAGTAAGTTTACATAGTGCGGGAGGAGGAGGTTGGGGAGATCCTTTAGATAGAGACCCGGAACTAGTTC
TACAAGACGTAAAAAATGAATATATTACACATGATATAGCACAAAAAGTTTATGGTGTAGTAATTGAAAACGGCAAAGTA
AACTACGATAAGACATTAAACTTAAGAAATATTATGCGAAGATCTAAGATACTTAGTGGGACTGTTCAATAA

Upstream 100 bases:

>100_bases
CAGCAATTATAGAAGATCCTACTTCTACATCGTTAGTATTAGAAGGGCAAACGGGAATGTTAGATAGTTATGGCAATCTA
ATTATTGAGAGGGATTAATA

Downstream 100 bases:

>100_bases
ACATCAATAGAATGGCATTGTAGAGCAATCATTTTTTATATTTTTTATAGAGAATAAACAGTTTTTATAGTATTTTATTA
GGTTAACCCTTGACCATAAT

Product: N-methylhydantoinase B (huyB-1)

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MVDPFTLEIIKNGLVVASEEMFYAFGRTAKSPVIYEVLDYAVGITDSKGRGLVAQAPGVPGFSGVLDFTIREVLGKWKSD
INPGDVIATNIPYSGGTHLNDVALIMPMFTKDTLVGFIVNKGHWSEIGGMHFGSWSSDATEIFQEGLQLPNIKLYIGGKP
NRDVIDIISANSRMPNATLGDMEAQIASMKIAARRINTLIEKYGLDNVIEAMDKLIEDGVKLSKLKLAKLPKGKFEAEDY
IDDDGISDNPVYVKVRVSITDNEFIVDFTGSSNQVNGPINSPLPATVSAVRETYMAITDPHALPNAGFFSPLKVIAPEGS
IFNPRKPAPTSTYWESMSYATDLVWKALAPYIPDKLSAGHFLSILATILGGYDEKTGEPFAIVEPQPGGWGGCNDQDGES
ALVASGDGETYIASAEVYERKMPILVERESLNTEDGPGHGKYRGGLGIIKDYVVLSKEAYFTVSIGRSKFPPWGINGGFS
GPPNYAVIYKKGEKPRVARKIAAIKLEYGDKVSLHSAGGGGWGDPLDRDPELVLQDVKNEYITHDIAQKVYGVVIENGKV
NYDKTLNLRNIMRRSKILSGTVQ

Sequences:

>Translated_583_residues
MVDPFTLEIIKNGLVVASEEMFYAFGRTAKSPVIYEVLDYAVGITDSKGRGLVAQAPGVPGFSGVLDFTIREVLGKWKSD
INPGDVIATNIPYSGGTHLNDVALIMPMFTKDTLVGFIVNKGHWSEIGGMHFGSWSSDATEIFQEGLQLPNIKLYIGGKP
NRDVIDIISANSRMPNATLGDMEAQIASMKIAARRINTLIEKYGLDNVIEAMDKLIEDGVKLSKLKLAKLPKGKFEAEDY
IDDDGISDNPVYVKVRVSITDNEFIVDFTGSSNQVNGPINSPLPATVSAVRETYMAITDPHALPNAGFFSPLKVIAPEGS
IFNPRKPAPTSTYWESMSYATDLVWKALAPYIPDKLSAGHFLSILATILGGYDEKTGEPFAIVEPQPGGWGGCNDQDGES
ALVASGDGETYIASAEVYERKMPILVERESLNTEDGPGHGKYRGGLGIIKDYVVLSKEAYFTVSIGRSKFPPWGINGGFS
GPPNYAVIYKKGEKPRVARKIAAIKLEYGDKVSLHSAGGGGWGDPLDRDPELVLQDVKNEYITHDIAQKVYGVVIENGKV
NYDKTLNLRNIMRRSKILSGTVQ
>Mature_583_residues
MVDPFTLEIIKNGLVVASEEMFYAFGRTAKSPVIYEVLDYAVGITDSKGRGLVAQAPGVPGFSGVLDFTIREVLGKWKSD
INPGDVIATNIPYSGGTHLNDVALIMPMFTKDTLVGFIVNKGHWSEIGGMHFGSWSSDATEIFQEGLQLPNIKLYIGGKP
NRDVIDIISANSRMPNATLGDMEAQIASMKIAARRINTLIEKYGLDNVIEAMDKLIEDGVKLSKLKLAKLPKGKFEAEDY
IDDDGISDNPVYVKVRVSITDNEFIVDFTGSSNQVNGPINSPLPATVSAVRETYMAITDPHALPNAGFFSPLKVIAPEGS
IFNPRKPAPTSTYWESMSYATDLVWKALAPYIPDKLSAGHFLSILATILGGYDEKTGEPFAIVEPQPGGWGGCNDQDGES
ALVASGDGETYIASAEVYERKMPILVERESLNTEDGPGHGKYRGGLGIIKDYVVLSKEAYFTVSIGRSKFPPWGINGGFS
GPPNYAVIYKKGEKPRVARKIAAIKLEYGDKVSLHSAGGGGWGDPLDRDPELVLQDVKNEYITHDIAQKVYGVVIENGKV
NYDKTLNLRNIMRRSKILSGTVQ

Specific function: Unknown

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=548, Percent_Identity=27.1897810218978, Blast_Score=181, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI133901900, Length=548, Percent_Identity=27.7372262773723, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI133901902, Length=514, Percent_Identity=27.0428015564202, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322634, Length=550, Percent_Identity=27.6363636363636, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI45550492, Length=565, Percent_Identity=26.1946902654867, Blast_Score=169, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 63397; Mature: 63397

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDPFTLEIIKNGLVVASEEMFYAFGRTAKSPVIYEVLDYAVGITDSKGRGLVAQAPGVP
CCCCEEHHHHHCCCEEEEHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCC
GFSGVLDFTIREVLGKWKSDINPGDVIATNIPYSGGTHLNDVALIMPMFTKDTLVGFIVN
CHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEEEHHCCCCEEEEEEC
KGHWSEIGGMHFGSWSSDATEIFQEGLQLPNIKLYIGGKPNRDVIDIISANSRMPNATLG
CCCCHHHCCEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCC
DMEAQIASMKIAARRINTLIEKYGLDNVIEAMDKLIEDGVKLSKLKLAKLPKGKFEAEDY
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCHHHC
IDDDGISDNPVYVKVRVSITDNEFIVDFTGSSNQVNGPINSPLPATVSAVRETYMAITDP
CCCCCCCCCCEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHEECC
HALPNAGFFSPLKVIAPEGSIFNPRKPAPTSTYWESMSYATDLVWKALAPYIPDKLSAGH
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
FLSILATILGGYDEKTGEPFAIVEPQPGGWGGCNDQDGESALVASGDGETYIASAEVYER
HHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCEEEEEHHHHHC
KMPILVERESLNTEDGPGHGKYRGGLGIIKDYVVLSKEAYFTVSIGRSKFPPWGINGGFS
CCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHEEECCEEEEEEECCCCCCCCCCCCCCC
GPPNYAVIYKKGEKPRVARKIAAIKLEYGDKVSLHSAGGGGWGDPLDRDPELVLQDVKNE
CCCCEEEEEECCCCCHHHHHEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
YITHDIAQKVYGVVIENGKVNYDKTLNLRNIMRRSKILSGTVQ
HHHHHHHHHHEEEEEECCEECCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVDPFTLEIIKNGLVVASEEMFYAFGRTAKSPVIYEVLDYAVGITDSKGRGLVAQAPGVP
CCCCEEHHHHHCCCEEEEHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCC
GFSGVLDFTIREVLGKWKSDINPGDVIATNIPYSGGTHLNDVALIMPMFTKDTLVGFIVN
CHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCEEEEEHHCCCCEEEEEEC
KGHWSEIGGMHFGSWSSDATEIFQEGLQLPNIKLYIGGKPNRDVIDIISANSRMPNATLG
CCCCHHHCCEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCC
DMEAQIASMKIAARRINTLIEKYGLDNVIEAMDKLIEDGVKLSKLKLAKLPKGKFEAEDY
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCHHHC
IDDDGISDNPVYVKVRVSITDNEFIVDFTGSSNQVNGPINSPLPATVSAVRETYMAITDP
CCCCCCCCCCEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHEECC
HALPNAGFFSPLKVIAPEGSIFNPRKPAPTSTYWESMSYATDLVWKALAPYIPDKLSAGH
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
FLSILATILGGYDEKTGEPFAIVEPQPGGWGGCNDQDGESALVASGDGETYIASAEVYER
HHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCEEEEEHHHHHC
KMPILVERESLNTEDGPGHGKYRGGLGIIKDYVVLSKEAYFTVSIGRSKFPPWGINGGFS
CCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHEEECCEEEEEEECCCCCCCCCCCCCCC
GPPNYAVIYKKGEKPRVARKIAAIKLEYGDKVSLHSAGGGGWGDPLDRDPELVLQDVKNE
CCCCEEEEEECCCCCHHHHHEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
YITHDIAQKVYGVVIENGKVNYDKTLNLRNIMRRSKILSGTVQ
HHHHHHHHHHEEEEEECCEECCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]