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The map label for this gene is mdlC [H]

Identifier: 15898465

GI number: 15898465

Start: 1499121

End: 1500632

Strand: Reverse

Name: mdlC [H]

Synonym: SSO1647

Alternate gene names: 15898465

Gene position: 1500632-1499121 (Counterclockwise)

Preceding gene: 15898466

Following gene: 15898460

Centisome position: 50.15

GC content: 36.51

Gene sequence:

>1512_bases
ATGAAAGTAGGAAATGCGTTGTTTAAGCTGATGAGTGAATTAGGAATTAGGCAAGTATTTGGAAACCCTGGTACTACAGA
ACTTAGCTTTCTAAAACACATGCCAAAAGACTTCAACTACTACCTAGCGCTCCATGACGGGATTTCGGTAGGAATGGCTG
AGGGTTATTACTTTGCAACGAGAAAACCTCAAATAGTTAATTTACATTCCTCACCGGGATTAACCAACGCTATGGGTTTT
ATATATGAGGCTCTTATTAGCAGAATTCCCCTTATCGTATTGGTTGGGCAACAGTACTTGTATAGATTGATAGATGAACC
AGTCCTCTACGGCGATTTTATAAAGATCTCTCAAGGAGTCGTTAAATCAGCATATGAGATAAGAAGTGAAAAAGACGCAA
TAAAAACGTTCATCAGAGCTTATAAGGAGAGTATTACTCCTCCATATGGTCCAGTGCTCATATCCTTACCTCAAGACATT
CCAGATATGGAAGTGAGTGAAGGAGAGAAAGTAGATATACCCAAATATTTTGTAAGTGGCACATGTGATACGTCTGCAAT
AGAATTTGTATTAGATAAAGTAAAGAGCGCTAGCTCGATTGCAATAGTGGCTGGATATGAAGTTTCCATATTTAGCGCTC
ATGAGGAGTTAGTTAGATTAGCTGAGAAACTTAACGCTCCAATTTACACTGAACCATATTTGAGTATATTTCCAATTGAC
TCCTCTAATATTTTGTTTAAAGGCCCCCTATCCAGATATAAGGCTTCTGATGTAGTGAAGGAATTAGAAAAATATGATCT
TGTTTTAGTCATTGGTGGATGGTTAAATTACGTTGTATTCCCAGACGTGGATATAAGGTTGAATATAGTGGAGGTAACTA
GTGACTTTAAAGAGGCGTCAAAGAGGAAATGGGATACTATTGTCTGTAATCCAAAAGATTTCCTCATCAAGCTTTACAAT
ATGTTATATAAAGGTCTAAACAAAAACATTATTAAACGTGAGAATAGGGATCTAGAGTTACAAGGTGATTTTATCACCGA
AGTTTTCAAAGAAATGAAAGGTTATTTGGATAAATATACAATATTTGCAGAAATTCCTACTTATAGGGATACTTTAATAA
AGATTATTGAACTAAAACCATCCTCTCTCTACATAACGAGATCTGGATTATTAGGGTGGGCACTCTCTGCATTGGTAGGT
TATAGTATAAATGGGGCTAAAGTCTTGGCAATAATTGGAGATGGAAGTTTCAATTATACTCCTCAAGCATTATGGAGTGC
CGTAAAATACAGTACTAGATTAAAAGTGATTGTAATTAATAACGAGGGATATGCGTCACTGTCAAGGCATGGTGTGGAGG
CAGATTGGCTGTTTCCATCAACTTCGCCTTGGAAAGTTGCATTAGCCTATGGATTTGAGGCTAAGGAATCAAGGGATATA
AAAAACGATTTGAAATGGTTATTCGAAGATGATAAGAGAAAATTGCTGGAAATTAGATTAGCTAGGCACTAA

Upstream 100 bases:

>100_bases
AGACGGTACGCTAAGAAGATTTAAATCCTTGTGGCAAGTCGTTTCACCGAAAAGGGAAATATAATTCACTTTTAAAATTA
TTAACTCGTATTATTTAGCC

Downstream 100 bases:

>100_bases
GACTGCCCTTCCTAAAATCCTTCCAGCTTTCAACTCCTCAAGTGCGCTATTAGCCTCCTCTAAGGGGAACGTCTTTACCT
TTAATTTTACCTTATTTTGA

Product: benzoylformate decarboxylase (mdlC)

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MKVGNALFKLMSELGIRQVFGNPGTTELSFLKHMPKDFNYYLALHDGISVGMAEGYYFATRKPQIVNLHSSPGLTNAMGF
IYEALISRIPLIVLVGQQYLYRLIDEPVLYGDFIKISQGVVKSAYEIRSEKDAIKTFIRAYKESITPPYGPVLISLPQDI
PDMEVSEGEKVDIPKYFVSGTCDTSAIEFVLDKVKSASSIAIVAGYEVSIFSAHEELVRLAEKLNAPIYTEPYLSIFPID
SSNILFKGPLSRYKASDVVKELEKYDLVLVIGGWLNYVVFPDVDIRLNIVEVTSDFKEASKRKWDTIVCNPKDFLIKLYN
MLYKGLNKNIIKRENRDLELQGDFITEVFKEMKGYLDKYTIFAEIPTYRDTLIKIIELKPSSLYITRSGLLGWALSALVG
YSINGAKVLAIIGDGSFNYTPQALWSAVKYSTRLKVIVINNEGYASLSRHGVEADWLFPSTSPWKVALAYGFEAKESRDI
KNDLKWLFEDDKRKLLEIRLARH

Sequences:

>Translated_503_residues
MKVGNALFKLMSELGIRQVFGNPGTTELSFLKHMPKDFNYYLALHDGISVGMAEGYYFATRKPQIVNLHSSPGLTNAMGF
IYEALISRIPLIVLVGQQYLYRLIDEPVLYGDFIKISQGVVKSAYEIRSEKDAIKTFIRAYKESITPPYGPVLISLPQDI
PDMEVSEGEKVDIPKYFVSGTCDTSAIEFVLDKVKSASSIAIVAGYEVSIFSAHEELVRLAEKLNAPIYTEPYLSIFPID
SSNILFKGPLSRYKASDVVKELEKYDLVLVIGGWLNYVVFPDVDIRLNIVEVTSDFKEASKRKWDTIVCNPKDFLIKLYN
MLYKGLNKNIIKRENRDLELQGDFITEVFKEMKGYLDKYTIFAEIPTYRDTLIKIIELKPSSLYITRSGLLGWALSALVG
YSINGAKVLAIIGDGSFNYTPQALWSAVKYSTRLKVIVINNEGYASLSRHGVEADWLFPSTSPWKVALAYGFEAKESRDI
KNDLKWLFEDDKRKLLEIRLARH
>Mature_503_residues
MKVGNALFKLMSELGIRQVFGNPGTTELSFLKHMPKDFNYYLALHDGISVGMAEGYYFATRKPQIVNLHSSPGLTNAMGF
IYEALISRIPLIVLVGQQYLYRLIDEPVLYGDFIKISQGVVKSAYEIRSEKDAIKTFIRAYKESITPPYGPVLISLPQDI
PDMEVSEGEKVDIPKYFVSGTCDTSAIEFVLDKVKSASSIAIVAGYEVSIFSAHEELVRLAEKLNAPIYTEPYLSIFPID
SSNILFKGPLSRYKASDVVKELEKYDLVLVIGGWLNYVVFPDVDIRLNIVEVTSDFKEASKRKWDTIVCNPKDFLIKLYN
MLYKGLNKNIIKRENRDLELQGDFITEVFKEMKGYLDKYTIFAEIPTYRDTLIKIIELKPSSLYITRSGLLGWALSALVG
YSINGAKVLAIIGDGSFNYTPQALWSAVKYSTRLKVIVINNEGYASLSRHGVEADWLFPSTSPWKVALAYGFEAKESRDI
KNDLKWLFEDDKRKLLEIRLARH

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=463, Percent_Identity=22.0302375809935, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=230, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 56981; Mature: 56981

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGNALFKLMSELGIRQVFGNPGTTELSFLKHMPKDFNYYLALHDGISVGMAEGYYFAT
CCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCCEECCCCCEEEEC
RKPQIVNLHSSPGLTNAMGFIYEALISRIPLIVLVGQQYLYRLIDEPVLYGDFIKISQGV
CCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCEECCHHHHHHHH
VKSAYEIRSEKDAIKTFIRAYKESITPPYGPVLISLPQDIPDMEVSEGEKVDIPKYFVSG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCEECCCHHEECC
TCDTSAIEFVLDKVKSASSIAIVAGYEVSIFSAHEELVRLAEKLNAPIYTEPYLSIFPID
CCCHHHHHHHHHHHCCCCCEEEEECCEEEEEHHHHHHHHHHHHHCCCEEECCCEEEEECC
SSNILFKGPLSRYKASDVVKELEKYDLVLVIGGWLNYVVFPDVDIRLNIVEVTSDFKEAS
CCCEEEECCHHHHHHHHHHHHHHHCCEEEEEECCCCEEECCCCCEEEEEEEEHHHHHHHH
KRKWDTIVCNPKDFLIKLYNMLYKGLNKNIIKRENRDLELQGDFITEVFKEMKGYLDKYT
CCCCCEEEECCHHHHHHHHHHHHHCCCCCHHHCCCCCEEECCHHHHHHHHHHHHHHHHEE
IFAEIPTYRDTLIKIIELKPSSLYITRSGLLGWALSALVGYSINGAKVLAIIGDGSFNYT
EEEECCCHHHHHEEEEEECCCEEEEECCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCC
PQALWSAVKYSTRLKVIVINNEGYASLSRHGVEADWLFPSTSPWKVALAYGFEAKESRDI
HHHHHHHHHHCCEEEEEEECCCCCHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCCCH
KNDLKWLFEDDKRKLLEIRLARH
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVGNALFKLMSELGIRQVFGNPGTTELSFLKHMPKDFNYYLALHDGISVGMAEGYYFAT
CCCHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCCEECCCCCEEEEC
RKPQIVNLHSSPGLTNAMGFIYEALISRIPLIVLVGQQYLYRLIDEPVLYGDFIKISQGV
CCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCEECCHHHHHHHH
VKSAYEIRSEKDAIKTFIRAYKESITPPYGPVLISLPQDIPDMEVSEGEKVDIPKYFVSG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCEECCCHHEECC
TCDTSAIEFVLDKVKSASSIAIVAGYEVSIFSAHEELVRLAEKLNAPIYTEPYLSIFPID
CCCHHHHHHHHHHHCCCCCEEEEECCEEEEEHHHHHHHHHHHHHCCCEEECCCEEEEECC
SSNILFKGPLSRYKASDVVKELEKYDLVLVIGGWLNYVVFPDVDIRLNIVEVTSDFKEAS
CCCEEEECCHHHHHHHHHHHHHHHCCEEEEEECCCCEEECCCCCEEEEEEEEHHHHHHHH
KRKWDTIVCNPKDFLIKLYNMLYKGLNKNIIKRENRDLELQGDFITEVFKEMKGYLDKYT
CCCCCEEEECCHHHHHHHHHHHHHCCCCCHHHCCCCCEEECCHHHHHHHHHHHHHHHHEE
IFAEIPTYRDTLIKIIELKPSSLYITRSGLLGWALSALVGYSINGAKVLAIIGDGSFNYT
EEEECCCHHHHHEEEEEECCCEEEEECCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCC
PQALWSAVKYSTRLKVIVINNEGYASLSRHGVEADWLFPSTSPWKVALAYGFEAKESRDI
HHHHHHHHHHCCEEEEEEECCCCCHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCCCH
KNDLKWLFEDDKRKLLEIRLARH
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]