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The map label for this gene is gcvH2

Identifier: 15897929

GI number: 15897929

Start: 919947

End: 920393

Strand: Reverse

Name: gcvH2

Synonym: SSO1061

Alternate gene names: 15897929

Gene position: 920393-919947 (Counterclockwise)

Preceding gene: 15897931

Following gene: 15897928

Centisome position: 30.76

GC content: 38.26

Gene sequence:

>447_bases
ATGGTAGTTGAATCGAACTGTGAAATACCAGAAAATCTTTATTACTTTATAGAAGGTAAAAACACAGTTTGGGCTAAATT
AGAGAGCCCAGATACAATCGTTGTAGGAATAACTGATCTTGCACAAACGATGGCAGGAAAGATAGTTAAAGTAAGGATAA
AGAAAAAAGGCACCAAGGTAGAGAAGGGAAGACCAGTAGCCACTATGGAAAGTGGGAAGTGGGCTGGACCAGTTCCGGCA
CCAGTAACTGGAGAAGTTGTTGAGGTTAATGCAGAGGCTGAGAAAAGTCCAATCATTATAAATCAAGATCCATATGGAAA
AGGATGGTTAGTAAAGATGAAGATGAGTAATCCAGAGGAGTTAAAACAACTATTTAGTGGCCAAGCAGCTATACAGAAAT
TGAAGGAATTAATAGCCTCTGAAAAATTAACATGCAAGAGGCTATAA

Upstream 100 bases:

>100_bases
CTATCTGAAAAATAAGATTTCATTATTCTCATTTTTCTGATAACTGCTTTCTAAAGCAAACTTTTTTAGTTAATTAGACT
TATTATATTACTGACCAAAC

Downstream 100 bases:

>100_bases
AATGAGTTGGAGATTTGTCTCTCTCCCTCCTCAAGATGGCTATCACATGGTAACTTCATTTGTATCGGTAGCGGATTATG
TAAATAGAGGAGGAAAAAAT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MVVESNCEIPENLYYFIEGKNTVWAKLESPDTIVVGITDLAQTMAGKIVKVRIKKKGTKVEKGRPVATMESGKWAGPVPA
PVTGEVVEVNAEAEKSPIIINQDPYGKGWLVKMKMSNPEELKQLFSGQAAIQKLKELIASEKLTCKRL

Sequences:

>Translated_148_residues
MVVESNCEIPENLYYFIEGKNTVWAKLESPDTIVVGITDLAQTMAGKIVKVRIKKKGTKVEKGRPVATMESGKWAGPVPA
PVTGEVVEVNAEAEKSPIIINQDPYGKGWLVKMKMSNPEELKQLFSGQAAIQKLKELIASEKLTCKRL
>Mature_148_residues
MVVESNCEIPENLYYFIEGKNTVWAKLESPDTIVVGITDLAQTMAGKIVKVRIKKKGTKVEKGRPVATMESGKWAGPVPA
PVTGEVVEVNAEAEKSPIIINQDPYGKGWLVKMKMSNPEELKQLFSGQAAIQKLKELIASEKLTCKRL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=103, Percent_Identity=36.8932038834951, Blast_Score=76, Evalue=1e-14,
Organism=Homo sapiens, GI89057342, Length=103, Percent_Identity=35.9223300970874, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789271, Length=121, Percent_Identity=38.0165289256198, Blast_Score=83, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17551294, Length=92, Percent_Identity=38.0434782608696, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507493, Length=93, Percent_Identity=36.5591397849462, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319272, Length=106, Percent_Identity=35.8490566037736, Blast_Score=71, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17865652, Length=103, Percent_Identity=36.8932038834951, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH2_SULSO (Q97Z69)

Other databases:

- EMBL:   AE006641
- PIR:   E90258
- RefSeq:   NP_342534.1
- ProteinModelPortal:   Q97Z69
- GeneID:   1454103
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO1061
- NMPDR:   fig|273057.1.peg.957
- HOGENOM:   HBG693789
- OMA:   IPENLLY
- ProtClustDB:   PRK13380
- BioCyc:   SSOL273057:SSO1061-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR002930
- InterPro:   IPR011053
- PANTHER:   PTHR11715

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 16299; Mature: 16299

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50968 BIOTINYL_LIPOYL; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVESNCEIPENLYYFIEGKNTVWAKLESPDTIVVGITDLAQTMAGKIVKVRIKKKGTKV
CEECCCCCCCCCEEEEEECCCEEEEEECCCCEEEEEHHHHHHHHHCEEEEEEEECCCCCC
EKGRPVATMESGKWAGPVPAPVTGEVVEVNAEAEKSPIIINQDPYGKGWLVKMKMSNPEE
CCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEECCCHHH
LKQLFSGQAAIQKLKELIASEKLTCKRL
HHHHHCCHHHHHHHHHHHHCCCCEECCC
>Mature Secondary Structure
MVVESNCEIPENLYYFIEGKNTVWAKLESPDTIVVGITDLAQTMAGKIVKVRIKKKGTKV
CEECCCCCCCCCEEEEEECCCEEEEEECCCCEEEEEHHHHHHHHHCEEEEEEEECCCCCC
EKGRPVATMESGKWAGPVPAPVTGEVVEVNAEAEKSPIIINQDPYGKGWLVKMKMSNPEE
CCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEECCCHHH
LKQLFSGQAAIQKLKELIASEKLTCKRL
HHHHHCCHHHHHHHHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726