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The map label for this gene is hycD [H]

Identifier: 15897894

GI number: 15897894

Start: 884757

End: 885719

Strand: Reverse

Name: hycD [H]

Synonym: SSO1026

Alternate gene names: 15897894

Gene position: 885719-884757 (Counterclockwise)

Preceding gene: 15897902

Following gene: 15897893

Centisome position: 29.6

GC content: 38.21

Gene sequence:

>963_bases
GTGGGAAGTATGGCGCCAAATGAAGCTCAGATAGTAGTTTTAACCATAACTCAAGTTATTGTAGTTATTCTACTATCCCC
ATTATATCAAGGAATTTACGATAGGGGAAAAGCCATAATAGAAGGGCGTAAAGGCCCCAGTGTTCTGCAACCTTACTATG
ATATAATTAAACTACTAAGAAAAGAAACCGTTATTTCAAATAACTCATTATCTCTATTTGTTTATGCTCCTTACATAGTT
TTTGGGATTTATCTCCTTATTTCCTTCGTCATCCCAGTAGTTTATCCAGTGCCCATCTTACTCACCCCTACGGTGGATTT
CTTAGGGGGAGCATTACTCTTCTCCTTAGCTGCGTTCATAAAAATAATTGCCTCCCTAGAAAGTGGAAGTAATTTCGTAG
CATTAGGAGTAAGTAGAATTCTCTCTTTCACTTTCTTATCTGAAGCTACACTGATAACGGTTTTCTTCGGCGTTGCACTA
ATAACTGGTACAAACAACCCTTACGTAACATTAGATTACGTTTCCCAAAGCCTCTCTCATTACTTTCAACTAGACCACAT
CTTCGTATCGATCTCATTCTTCATGTTATGGTTATTCGAAACCGGTAAATTACCCGTAGAGAGTCCTGGACTAAGCGAAA
TGGGTATGATAGACGATGGAGTTCTCTACGAATATAGCGGAAAATTACTTGCAATACTAAAGTGGGGTTCTTACATCAAA
CAATATCTATTAGGCTCAGTACTCCTAAACGTATTTATATTCCCTTGGTTTTTGCAAGTTGGAATTATAGGCTCTTTAAT
AGACATTGCGGTGATGTTCGGCAAATGGTTACTCCTAATCCTTATTAGCGTTATAATAAACACCACCCTAGCTAAATTAA
GGCTATTTAAAGTTCAGGATTATCTCGCAGTGGCGTTCCTCCTTTCCCTACTTTCATTAACGCTTACTGTCTTGTTGGGG
TGA

Upstream 100 bases:

>100_bases
TTTACTCAATGAAGTAGGGGATGGCGTCCCCCTGGGGATAACCCCGAACCGCCTCTTGATTAATGATAGGGGCTGATGAC
GCCTACTTCACCACAAAAGG

Downstream 100 bases:

>100_bases
AGGTATAGTGATTAACTCAGAGATCCTAGAACTACTCTCAACGTTCATATTAATTTCAGCCTTTTACATTCAAGGACAAG
CTTACTTTAAACCAGCAATA

Product: formate hydrogenlyase subunit 4 (hycD)

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MGSMAPNEAQIVVLTITQVIVVILLSPLYQGIYDRGKAIIEGRKGPSVLQPYYDIIKLLRKETVISNNSLSLFVYAPYIV
FGIYLLISFVIPVVYPVPILLTPTVDFLGGALLFSLAAFIKIIASLESGSNFVALGVSRILSFTFLSEATLITVFFGVAL
ITGTNNPYVTLDYVSQSLSHYFQLDHIFVSISFFMLWLFETGKLPVESPGLSEMGMIDDGVLYEYSGKLLAILKWGSYIK
QYLLGSVLLNVFIFPWFLQVGIIGSLIDIAVMFGKWLLLILISVIINTTLAKLRLFKVQDYLAVAFLLSLLSLTLTVLLG

Sequences:

>Translated_320_residues
MGSMAPNEAQIVVLTITQVIVVILLSPLYQGIYDRGKAIIEGRKGPSVLQPYYDIIKLLRKETVISNNSLSLFVYAPYIV
FGIYLLISFVIPVVYPVPILLTPTVDFLGGALLFSLAAFIKIIASLESGSNFVALGVSRILSFTFLSEATLITVFFGVAL
ITGTNNPYVTLDYVSQSLSHYFQLDHIFVSISFFMLWLFETGKLPVESPGLSEMGMIDDGVLYEYSGKLLAILKWGSYIK
QYLLGSVLLNVFIFPWFLQVGIIGSLIDIAVMFGKWLLLILISVIINTTLAKLRLFKVQDYLAVAFLLSLLSLTLTVLLG
>Mature_319_residues
GSMAPNEAQIVVLTITQVIVVILLSPLYQGIYDRGKAIIEGRKGPSVLQPYYDIIKLLRKETVISNNSLSLFVYAPYIVF
GIYLLISFVIPVVYPVPILLTPTVDFLGGALLFSLAAFIKIIASLESGSNFVALGVSRILSFTFLSEATLITVFFGVALI
TGTNNPYVTLDYVSQSLSHYFQLDHIFVSISFFMLWLFETGKLPVESPGLSEMGMIDDGVLYEYSGKLLAILKWGSYIKQ
YLLGSVLLNVFIFPWFLQVGIIGSLIDIAVMFGKWLLLILISVIINTTLAKLRLFKVQDYLAVAFLLSLLSLTLTVLLG

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=288, Percent_Identity=32.6388888888889, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082115, Length=254, Percent_Identity=27.1653543307087, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 35395; Mature: 35264

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSMAPNEAQIVVLTITQVIVVILLSPLYQGIYDRGKAIIEGRKGPSVLQPYYDIIKLLR
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHH
KETVISNNSLSLFVYAPYIVFGIYLLISFVIPVVYPVPILLTPTVDFLGGALLFSLAAFI
HHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHH
KIIASLESGSNFVALGVSRILSFTFLSEATLITVFFGVALITGTNNPYVTLDYVSQSLSH
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHH
YFQLDHIFVSISFFMLWLFETGKLPVESPGLSEMGMIDDGVLYEYSGKLLAILKWGSYIK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEHHHHHHHH
QYLLGSVLLNVFIFPWFLQVGIIGSLIDIAVMFGKWLLLILISVIINTTLAKLRLFKVQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLAVAFLLSLLSLTLTVLLG
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GSMAPNEAQIVVLTITQVIVVILLSPLYQGIYDRGKAIIEGRKGPSVLQPYYDIIKLLR
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHH
KETVISNNSLSLFVYAPYIVFGIYLLISFVIPVVYPVPILLTPTVDFLGGALLFSLAAFI
HHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHH
KIIASLESGSNFVALGVSRILSFTFLSEATLITVFFGVALITGTNNPYVTLDYVSQSLSH
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHH
YFQLDHIFVSISFFMLWLFETGKLPVESPGLSEMGMIDDGVLYEYSGKLLAILKWGSYIK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEHHHHHHHH
QYLLGSVLLNVFIFPWFLQVGIIGSLIDIAVMFGKWLLLILISVIINTTLAKLRLFKVQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLAVAFLLSLLSLTLTVLLG
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]