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The map label for this gene is ilvB-3 [H]

Identifier: 15897885

GI number: 15897885

Start: 868399

End: 870048

Strand: Reverse

Name: ilvB-3 [H]

Synonym: SSO1012

Alternate gene names: 15897885

Gene position: 870048-868399 (Counterclockwise)

Preceding gene: 15897886

Following gene: 15897884

Centisome position: 29.08

GC content: 34.73

Gene sequence:

>1650_bases
ATGCCATCAGTTGCAGAAGTAATAATAAGAGTATTAGAAGATAATGGAATTCAAAGAATATATGGAATTCCTGGAGATTC
CATTGACCCTTTAGTTGACGCGATAAGAAAATCTAAAGTAAAATACGTACAAGTAAGACATGAAGAAGGTGCAGCTTTAG
CTGCCTCGGTCGAAGCGAAAATAACTGGTAAGCCTTCAGCATGTATGGGTACTTCTGGTCCTGGATCAATCCATTTATTA
AATGGATTATACGATGCAAAAATGGATCATGCTCCAGTAATAGCGCTAACTGGACAAGTAGAGTCAGATATGATAGGTCA
CGATTATTTTCAAGAAGTTAACCTAACTAAGTTATTTGATGATGTGGCAGTATATAATCAAATTTTAATTAACCCAGAAA
ACGCGGAATATATAATAAGGAGGGCTATAAGAGAGGCTATTTCCAAAAGGGGAGTAGCTCACATAAATTTACCAGTAGAT
ATTCTCAGAAAGTCCTCAGAATATAAGGGTAGCAAGAATACTGAAGTAGGTAAAGTTAAATATTCGATAGATTTTTCTAG
AGCTAAGGAATTAATCAAAGAAAGTGAGAAACCAGTTTTACTAATTGGAGGAGGGACTAGAGGCCTAGGTAAAGAGATAA
ATAGGTTTGCTGAAAAAATAGGAGCACCAATAATATATACATTAAATGGTAAGGGGATTTTACCAGATTTAGATCCTAAA
GTTATGGGCGGAATAGGTCTTTTAGGAACTAAGCCTTCCATAGAGGCGATGGATAAGGCTGATTTATTAATAATGTTAGG
CGCATCATTTCCTTACGTTAATTTTCTAAATAAGAGTGCCAAAGTGATACAGGTTGATATAGATAATTCTAATATAGGTA
AGAGGTTAGATGTTAATCTCTCTTATCCGATTCCAGTTGCTGAGTTCCTAAATATAGATATCGAAGAGAAATCAGATAAA
TACTATGAAGAGTTAAAAGGAAAGAAGGAAGATTGGCTAGATTCTATAAGTAAGCAGGAGAATAGTTTAGATAAACCAAT
GAAACCTCAGAGAGTAGCTTATATAGTTTCCCAGAAGTGCAAGAAAGACGCAGTAATAGTTACTGATACTGGAAATGTAA
CTATGTGGACTGCTAGACACTTTAGAGCTTCAGGAGAGCAAACCTTTATATTTTCTGCTTGGCTAGGTTCAATGGGCATT
GGAGTCCCAGGAAGTGTAGGAGCTTCTTTTGCTGTAGAAAATAAAAGACAAGTTATTTCTTTTGTAGGAGATGGAGGTTT
TACTATGACTATGATGGAAATGATAACTGCTAAGAAATATGATCTTCCAGTTAAAATAATCGTTTATAATAATTCTAAAT
TAGGAATGATAAAATTTGAACAAGAAGTAATGGGGTACCCAGAATGGGGAGTCGATTTATATAACCCAGATTTCACAAAG
ATAGCTGAATCTATTGGATTTAAAGGATTTAGATTAGAAGAGCCAAAAGAGGCTGAGGAAATAATAGAAGATTTTCTAAA
CACTAAAGGACAGGCACTTTTAGATGCAATAGTAGATCCAAATGAGAGACCAATGCCACCTAAACTAACTTTTAAGCAAG
CTGGAGAATACGTTCTTTCAATATTTAGAGAGAAATTAGAGGGTATTTAA

Upstream 100 bases:

>100_bases
TTTTCCAAATATTTTTCTATATTTTGCATTAAGAGAAGTTATAAGCCAGTTGCGGCTATTATAAAAAAGACTATAAAGGA
ATAAGTAAAATATATATATT

Downstream 100 bases:

>100_bases
ATGATACTTATCTTAGGTGGAGGATTCGCAGGAGTTTCAGCTTATAATCAAAATAAAGAAAATTCCTTAGTAGTCGACAG
AAAGGACTATTTTCTTTTAA

Product: pyruvate dehydrogenase

Products: acetate; CO2; ferrocytochrome b1

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MPSVAEVIIRVLEDNGIQRIYGIPGDSIDPLVDAIRKSKVKYVQVRHEEGAALAASVEAKITGKPSACMGTSGPGSIHLL
NGLYDAKMDHAPVIALTGQVESDMIGHDYFQEVNLTKLFDDVAVYNQILINPENAEYIIRRAIREAISKRGVAHINLPVD
ILRKSSEYKGSKNTEVGKVKYSIDFSRAKELIKESEKPVLLIGGGTRGLGKEINRFAEKIGAPIIYTLNGKGILPDLDPK
VMGGIGLLGTKPSIEAMDKADLLIMLGASFPYVNFLNKSAKVIQVDIDNSNIGKRLDVNLSYPIPVAEFLNIDIEEKSDK
YYEELKGKKEDWLDSISKQENSLDKPMKPQRVAYIVSQKCKKDAVIVTDTGNVTMWTARHFRASGEQTFIFSAWLGSMGI
GVPGSVGASFAVENKRQVISFVGDGGFTMTMMEMITAKKYDLPVKIIVYNNSKLGMIKFEQEVMGYPEWGVDLYNPDFTK
IAESIGFKGFRLEEPKEAEEIIEDFLNTKGQALLDAIVDPNERPMPPKLTFKQAGEYVLSIFREKLEGI

Sequences:

>Translated_549_residues
MPSVAEVIIRVLEDNGIQRIYGIPGDSIDPLVDAIRKSKVKYVQVRHEEGAALAASVEAKITGKPSACMGTSGPGSIHLL
NGLYDAKMDHAPVIALTGQVESDMIGHDYFQEVNLTKLFDDVAVYNQILINPENAEYIIRRAIREAISKRGVAHINLPVD
ILRKSSEYKGSKNTEVGKVKYSIDFSRAKELIKESEKPVLLIGGGTRGLGKEINRFAEKIGAPIIYTLNGKGILPDLDPK
VMGGIGLLGTKPSIEAMDKADLLIMLGASFPYVNFLNKSAKVIQVDIDNSNIGKRLDVNLSYPIPVAEFLNIDIEEKSDK
YYEELKGKKEDWLDSISKQENSLDKPMKPQRVAYIVSQKCKKDAVIVTDTGNVTMWTARHFRASGEQTFIFSAWLGSMGI
GVPGSVGASFAVENKRQVISFVGDGGFTMTMMEMITAKKYDLPVKIIVYNNSKLGMIKFEQEVMGYPEWGVDLYNPDFTK
IAESIGFKGFRLEEPKEAEEIIEDFLNTKGQALLDAIVDPNERPMPPKLTFKQAGEYVLSIFREKLEGI
>Mature_548_residues
PSVAEVIIRVLEDNGIQRIYGIPGDSIDPLVDAIRKSKVKYVQVRHEEGAALAASVEAKITGKPSACMGTSGPGSIHLLN
GLYDAKMDHAPVIALTGQVESDMIGHDYFQEVNLTKLFDDVAVYNQILINPENAEYIIRRAIREAISKRGVAHINLPVDI
LRKSSEYKGSKNTEVGKVKYSIDFSRAKELIKESEKPVLLIGGGTRGLGKEINRFAEKIGAPIIYTLNGKGILPDLDPKV
MGGIGLLGTKPSIEAMDKADLLIMLGASFPYVNFLNKSAKVIQVDIDNSNIGKRLDVNLSYPIPVAEFLNIDIEEKSDKY
YEELKGKKEDWLDSISKQENSLDKPMKPQRVAYIVSQKCKKDAVIVTDTGNVTMWTARHFRASGEQTFIFSAWLGSMGIG
VPGSVGASFAVENKRQVISFVGDGGFTMTMMEMITAKKYDLPVKIIVYNNSKLGMIKFEQEVMGYPEWGVDLYNPDFTKI
AESIGFKGFRLEEPKEAEEIIEDFLNTKGQALLDAIVDPNERPMPPKLTFKQAGEYVLSIFREKLEGI

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=576, Percent_Identity=23.7847222222222, Blast_Score=156, Evalue=5e-38,
Organism=Escherichia coli, GI1787096, Length=558, Percent_Identity=33.6917562724014, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=30.7272727272727, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=28.0918727915194, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1786717, Length=523, Percent_Identity=25.4302103250478, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=24.0143369175627, Blast_Score=116, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17531299, Length=479, Percent_Identity=24.2171189979123, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=479, Percent_Identity=24.2171189979123, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17542570, Length=563, Percent_Identity=23.4458259325044, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=552, Percent_Identity=28.2608695652174, Blast_Score=211, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6320816, Length=548, Percent_Identity=23.1751824817518, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6320123, Length=496, Percent_Identity=22.5806451612903, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320588, Length=552, Percent_Identity=21.5579710144928, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19922626, Length=486, Percent_Identity=25.5144032921811, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2

Molecular weight: Translated: 60755; Mature: 60624

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVAEVIIRVLEDNGIQRIYGIPGDSIDPLVDAIRKSKVKYVQVRHEEGAALAASVEAK
CCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCEEEEEEEEE
ITGKPSACMGTSGPGSIHLLNGLYDAKMDHAPVIALTGQVESDMIGHDYFQEVNLTKLFD
ECCCCCCCCCCCCCCCEEEECCHHHCCCCCCCEEEEECCCCCCCCCCHHHHHCCHHHHHH
DVAVYNQILINPENAEYIIRRAIREAISKRGVAHINLPVDILRKSSEYKGSKNTEVGKVK
HHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHCCHHCCCCCCCEEEEEE
YSIDFSRAKELIKESEKPVLLIGGGTRGLGKEINRFAEKIGAPIIYTLNGKGILPDLDPK
EEECHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHH
VMGGIGLLGTKPSIEAMDKADLLIMLGASFPYVNFLNKSAKVIQVDIDNSNIGKRLDVNL
HHCCEEEECCCCCCHHCCCCCEEEEECCCCCHHHHHCCCCEEEEEEECCCCCCCEEEEEE
SYPIPVAEFLNIDIEEKSDKYYEELKGKKEDWLDSISKQENSLDKPMKPQRVAYIVSQKC
CCCCCHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
KKDAVIVTDTGNVTMWTARHFRASGEQTFIFSAWLGSMGIGVPGSVGASFAVENKRQVIS
CCCCEEEECCCCEEEEEEHHHCCCCCEEEEEEEHHHCCCCCCCCCCCCCEEECCCCEEEE
FVGDGGFTMTMMEMITAKKYDLPVKIIVYNNSKLGMIKFEQEVMGYPEWGVDLYNPDFTK
EECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEHHHHCCCCCCCCCCCCCCHHH
IAESIGFKGFRLEEPKEAEEIIEDFLNTKGQALLDAIVDPNERPMPPKLTFKQAGEYVLS
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IFREKLEGI
HHHHHHCCC
>Mature Secondary Structure 
PSVAEVIIRVLEDNGIQRIYGIPGDSIDPLVDAIRKSKVKYVQVRHEEGAALAASVEAK
CCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCEEEEEEEEE
ITGKPSACMGTSGPGSIHLLNGLYDAKMDHAPVIALTGQVESDMIGHDYFQEVNLTKLFD
ECCCCCCCCCCCCCCCEEEECCHHHCCCCCCCEEEEECCCCCCCCCCHHHHHCCHHHHHH
DVAVYNQILINPENAEYIIRRAIREAISKRGVAHINLPVDILRKSSEYKGSKNTEVGKVK
HHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHCCHHCCCCCCCEEEEEE
YSIDFSRAKELIKESEKPVLLIGGGTRGLGKEINRFAEKIGAPIIYTLNGKGILPDLDPK
EEECHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHH
VMGGIGLLGTKPSIEAMDKADLLIMLGASFPYVNFLNKSAKVIQVDIDNSNIGKRLDVNL
HHCCEEEECCCCCCHHCCCCCEEEEECCCCCHHHHHCCCCEEEEEEECCCCCCCEEEEEE
SYPIPVAEFLNIDIEEKSDKYYEELKGKKEDWLDSISKQENSLDKPMKPQRVAYIVSQKC
CCCCCHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
KKDAVIVTDTGNVTMWTARHFRASGEQTFIFSAWLGSMGIGVPGSVGASFAVENKRQVIS
CCCCEEEECCCCEEEEEEHHHCCCCCEEEEEEEHHHCCCCCCCCCCCCCEEECCCCEEEE
FVGDGGFTMTMMEMITAKKYDLPVKIIVYNNSKLGMIKFEQEVMGYPEWGVDLYNPDFTK
EECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEHHHHCCCCCCCCCCCCCCHHH
IAESIGFKGFRLEEPKEAEEIIEDFLNTKGQALLDAIVDPNERPMPPKLTFKQAGEYVLS
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IFREKLEGI
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.0012 {thiamine} 0.03 {sodium} 2 {ferricyanide}} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: pyruvate; ferricytochrome b1; H2O

Specific reaction: pyruvate + ferricytochrome b1 + H2O = acetate + CO2 + ferrocytochrome b1

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]