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The map label for this gene is gatA

Identifier: 15897836

GI number: 15897836

Start: 811637

End: 813073

Strand: Reverse

Name: gatA

Synonym: SSO0957

Alternate gene names: 15897836

Gene position: 813073-811637 (Counterclockwise)

Preceding gene: 15897837

Following gene: 15897835

Centisome position: 27.17

GC content: 36.74

Gene sequence:

>1437_bases
GTGAATAGAATTATGATAGTAGACTTAGTCAACGATTTGAGAAATAAGAACTTAGAAGCTGAAGAGTACATCGAGAGAAC
GTATGAGAAAATAGGCAAATATGACAAATATATTAATGCGTTTATAACTATCAGAAGTAAGGAAGAGGTATTAAAGGAAG
TAAAAGAAAGCATTAACAAAGGGGGTAAATTAGCAGGAGTCCTAATTGCAATTAAGGATAACATCTCGACTGAGGGAATT
AGAACTACTTGTGCGTCAAAGATGTTAGAAGATTACATACCTCCATACGATGCTACAGTGATAGAAAAATTAAAGAAGGA
AGGGGCAGTAATCATTGGAAAAACCAACATGGACGAATTCGCTATGGGTTCAACTACAGAAACTAGTTATTTCGGTCCTA
CTAGAAATCCTTGGGACTTAGAAAGAACTCCTGGGGGATCATCTGGAGGCAGCGGAGCAGCTCTAGCGGCTGGTTATGTC
GAATTGGCGTTAGGTAGTGACACTGGAGGTTCCATAAGAGCACCTGCAGCATATAATGCCATTTTTGGTTTAAAGCCGTC
TTATGGTACAGTGAGTAGATTTGGTCTAGTAGCTTACGCAAATAGTTTAGAACAAATAGGACCAATGGCTAGAAATGCCG
AAGACTTAGGATTGCTTTTCTCAATAATTGCTGGACCAGATGAGAGGGACGCTACTACAATTAACTTAAATCTAAATTTT
GAGTTAAAGGAACGAAGCGTAAAAAACGTTAGGATAGGTGTTCTTAGTGACATTTTAGAAATGTCGGAAAAACCAGTATC
CGGTGTGATAAAAGATGTTGTAAATAAACTATCTAGCGAAGGGGCTTTAATTGAAGATACTAAACTAGGTAATGCGGAAT
ACGCACTACCAGCATATTACATAATTGCAATGTCTGAAGCAAGCTCTAATTTGGCAAGATATGATGGAGTTAGGTATGGA
TACAGTAAGTATATGGAAGGGAATTGGCGTGAAGTTTATGCTAAAAATAGAGGAGAAGCGTTTGGTATGGAGGTAAAGAG
GAGGATTCTTCTTGGCAGTTTTATCTTAAGTGCTGGATATTATGAGGAATTTTACCTCAAGGCATTAAAGGTTAGAAATT
TAATCAAGAAGAACTTGGACGAATTATTCAAAAAGTATGATATTTTGCTCTCTCCTACAATGCCTATACTACCGCCTAAG
ATAGGTGAGGTAATTAATGATCCGGTTAGAATGTACGCAATGGATCTAAATACCGTGATAGCTAACTTAGCTGCAATACC
AGCACTATCAATGCCGGTAGGATTTTACAGTGACCTTCCAATAGGATTACAGTTAATGGGTAAATATTTGTCAGATATTT
ATTTAATTAACATATCATCCTTTATAGAGAGAAATATAACAAAATTGTACAATTTAACCGCATCAGTTAAGATCTAA

Upstream 100 bases:

>100_bases
CTAAGGAAAGATGTTCCAAGGGATCCATTAGACAGGGAAAATGCTCTTAAAAATGTGAAAAGAAAGGAAGATGGGTATAT
AGTAGGACCTAGAACTTACG

Downstream 100 bases:

>100_bases
TAATTTTTTAATCCTATGTGCGATAGAATGTACAATATGCAAAGTAATTTAAGGATCGATACAGTAAGTGAGATATTCAA
GGAGTTACAAAAAGAAAAGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNRIMIVDLVNDLRNKNLEAEEYIERTYEKIGKYDKYINAFITIRSKEEVLKEVKESINKGGKLAGVLIAIKDNISTEGI
RTTCASKMLEDYIPPYDATVIEKLKKEGAVIIGKTNMDEFAMGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGYV
ELALGSDTGGSIRAPAAYNAIFGLKPSYGTVSRFGLVAYANSLEQIGPMARNAEDLGLLFSIIAGPDERDATTINLNLNF
ELKERSVKNVRIGVLSDILEMSEKPVSGVIKDVVNKLSSEGALIEDTKLGNAEYALPAYYIIAMSEASSNLARYDGVRYG
YSKYMEGNWREVYAKNRGEAFGMEVKRRILLGSFILSAGYYEEFYLKALKVRNLIKKNLDELFKKYDILLSPTMPILPPK
IGEVINDPVRMYAMDLNTVIANLAAIPALSMPVGFYSDLPIGLQLMGKYLSDIYLINISSFIERNITKLYNLTASVKI

Sequences:

>Translated_478_residues
MNRIMIVDLVNDLRNKNLEAEEYIERTYEKIGKYDKYINAFITIRSKEEVLKEVKESINKGGKLAGVLIAIKDNISTEGI
RTTCASKMLEDYIPPYDATVIEKLKKEGAVIIGKTNMDEFAMGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGYV
ELALGSDTGGSIRAPAAYNAIFGLKPSYGTVSRFGLVAYANSLEQIGPMARNAEDLGLLFSIIAGPDERDATTINLNLNF
ELKERSVKNVRIGVLSDILEMSEKPVSGVIKDVVNKLSSEGALIEDTKLGNAEYALPAYYIIAMSEASSNLARYDGVRYG
YSKYMEGNWREVYAKNRGEAFGMEVKRRILLGSFILSAGYYEEFYLKALKVRNLIKKNLDELFKKYDILLSPTMPILPPK
IGEVINDPVRMYAMDLNTVIANLAAIPALSMPVGFYSDLPIGLQLMGKYLSDIYLINISSFIERNITKLYNLTASVKI
>Mature_478_residues
MNRIMIVDLVNDLRNKNLEAEEYIERTYEKIGKYDKYINAFITIRSKEEVLKEVKESINKGGKLAGVLIAIKDNISTEGI
RTTCASKMLEDYIPPYDATVIEKLKKEGAVIIGKTNMDEFAMGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGYV
ELALGSDTGGSIRAPAAYNAIFGLKPSYGTVSRFGLVAYANSLEQIGPMARNAEDLGLLFSIIAGPDERDATTINLNLNF
ELKERSVKNVRIGVLSDILEMSEKPVSGVIKDVVNKLSSEGALIEDTKLGNAEYALPAYYIIAMSEASSNLARYDGVRYG
YSKYMEGNWREVYAKNRGEAFGMEVKRRILLGSFILSAGYYEEFYLKALKVRNLIKKNLDELFKKYDILLSPTMPILPPK
IGEVINDPVRMYAMDLNTVIANLAAIPALSMPVGFYSDLPIGLQLMGKYLSDIYLINISSFIERNITKLYNLTASVKI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=494, Percent_Identity=36.6396761133603, Blast_Score=292, Evalue=6e-79,
Organism=Homo sapiens, GI195972892, Length=489, Percent_Identity=25.7668711656442, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI166795287, Length=159, Percent_Identity=36.4779874213836, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=471, Percent_Identity=36.5180467091295, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17537465, Length=458, Percent_Identity=27.0742358078603, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17556264, Length=169, Percent_Identity=32.5443786982249, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=264, Percent_Identity=28.7878787878788, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=153, Percent_Identity=30.718954248366, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=217, Percent_Identity=33.6405529953917, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=217, Percent_Identity=33.6405529953917, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538254, Length=213, Percent_Identity=30.5164319248826, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=468, Percent_Identity=34.4017094017094, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=435, Percent_Identity=32.8735632183908, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320448, Length=183, Percent_Identity=33.879781420765, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24648113, Length=466, Percent_Identity=39.0557939914163, Blast_Score=284, Evalue=9e-77,
Organism=Drosophila melanogaster, GI24652985, Length=482, Percent_Identity=27.3858921161826, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI19922090, Length=482, Percent_Identity=27.3858921161826, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24652981, Length=482, Percent_Identity=27.3858921161826, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24652983, Length=482, Percent_Identity=27.3858921161826, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45550774, Length=238, Percent_Identity=33.6134453781513, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648435, Length=241, Percent_Identity=33.6099585062241, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24648437, Length=241, Percent_Identity=33.6099585062241, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161078093, Length=492, Percent_Identity=27.4390243902439, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648441, Length=176, Percent_Identity=36.3636363636364, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648439, Length=176, Percent_Identity=36.3636363636364, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24644968, Length=241, Percent_Identity=29.4605809128631, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356731, Length=235, Percent_Identity=29.3617021276596, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_SULSO (Q97ZG1)

Other databases:

- EMBL:   AE006641
- PIR:   H90246
- RefSeq:   NP_342441.1
- ProteinModelPortal:   Q97ZG1
- SMR:   Q97ZG1
- GeneID:   1455198
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0957
- NMPDR:   fig|273057.1.peg.864
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- PhylomeDB:   Q97ZG1
- ProtClustDB:   PRK00012
- BioCyc:   SSOL273057:SSO0957-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52840; Mature: 52840

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 72-72 ACT_SITE 147-147 ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIMIVDLVNDLRNKNLEAEEYIERTYEKIGKYDKYINAFITIRSKEEVLKEVKESINK
CCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEHHEEEECHHHHHHHHHHHHCC
GGKLAGVLIAIKDNISTEGIRTTCASKMLEDYIPPYDATVIEKLKKEGAVIIGKTNMDEF
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCHH
AMGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGYVELALGSDTGGSIRAPAAYNA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEEEECCCCCCCEECCHHHHH
IFGLKPSYGTVSRFGLVAYANSLEQIGPMARNAEDLGLLFSIIAGPDERDATTINLNLNF
HCCCCCCCCCHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEEEE
ELKERSVKNVRIGVLSDILEMSEKPVSGVIKDVVNKLSSEGALIEDTKLGNAEYALPAYY
EECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEE
IIAMSEASSNLARYDGVRYGYSKYMEGNWREVYAKNRGEAFGMEVKRRILLGSFILSAGY
EEEECCCCCCHHHHCCCHHCHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
YEEFYLKALKVRNLIKKNLDELFKKYDILLSPTMPILPPKIGEVINDPVRMYAMDLNTVI
HHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHH
ANLAAIPALSMPVGFYSDLPIGLQLMGKYLSDIYLINISSFIERNITKLYNLTASVKI
HHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MNRIMIVDLVNDLRNKNLEAEEYIERTYEKIGKYDKYINAFITIRSKEEVLKEVKESINK
CCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEHHEEEECHHHHHHHHHHHHCC
GGKLAGVLIAIKDNISTEGIRTTCASKMLEDYIPPYDATVIEKLKKEGAVIIGKTNMDEF
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCHH
AMGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGYVELALGSDTGGSIRAPAAYNA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEEEECCCCCCCEECCHHHHH
IFGLKPSYGTVSRFGLVAYANSLEQIGPMARNAEDLGLLFSIIAGPDERDATTINLNLNF
HCCCCCCCCCHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEEEE
ELKERSVKNVRIGVLSDILEMSEKPVSGVIKDVVNKLSSEGALIEDTKLGNAEYALPAYY
EECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEE
IIAMSEASSNLARYDGVRYGYSKYMEGNWREVYAKNRGEAFGMEVKRRILLGSFILSAGY
EEEECCCCCCHHHHCCCHHCHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
YEEFYLKALKVRNLIKKNLDELFKKYDILLSPTMPILPPKIGEVINDPVRMYAMDLNTVI
HHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHH
ANLAAIPALSMPVGFYSDLPIGLQLMGKYLSDIYLINISSFIERNITKLYNLTASVKI
HHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726