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The map label for this gene is eno [H]

Identifier: 15897800

GI number: 15897800

Start: 777138

End: 778397

Strand: Reverse

Name: eno [H]

Synonym: SSO0913

Alternate gene names: 15897800

Gene position: 778397-777138 (Counterclockwise)

Preceding gene: 15897804

Following gene: 15897798

Centisome position: 26.01

GC content: 34.6

Gene sequence:

>1260_bases
ATGATTAACCGTTTTTCCATAGAGAAGGTTAAGGGATTAGAAATCGTAGATTCTAGAGGTAATCCCACTATAAGAGTTTT
CATAAGAACTAGTGATGGTGTCGAATCCTTTGGAGACGCACCAGCAGGGGCTTCTAAAGGGACAAGAGAGGCGGTAGAAG
TTAGGGATGAAAATGGGCTTACAGTAAAGAGGGCAGTAGACATTGTAAATTACATAATAGATCCTGCATTACATGGAATT
GATGTAAGAGAACAAGGGATAATCGACAAATTACTAAAAGATATAGACTCCACTGAGAATAAGTCTAAATTAGGAGGAAA
CACAATAATTGCAACATCAATAGCTGCATTAAAGACTGCTTCTAAGGCCTTAGGTCTAGAGGTTTTTAAATACATATCTG
GGCCTAGATTACCTAAAATCCCAATACCTTTACTTAATATAATAAATGGCGGTTTACATGCTGGAAATAAGCTAAAAATA
CAAGAATTCATTATAGTGCCAATTAAGTTCAATACTTTTAAAGAAGCTCTTTTCGCTGCGATAGACGTTTATAGAACCCT
AAAAGGGTTAATAACGGAGAGGTATGGTAAAATTTACACAGCAGTTGGAGATGAAGGGGGATTCTCTCCACCTTTAGAAG
ATACTAGAGAGGCCTTGGATCTAATATATACTTCCATAAATAATGCAGGTTATGAAGGAAAAATATATATGGGAATGGAT
GCTGCAGGGAGCGATTTCTACGATAGTAAAAAAGAGAAATATATAATTGATGGTAGAGAATTGGATCCTAATCAATTACT
TGAATTTTATCTTGACTTAGTTAAACAATATCCCATAGTGTACTTGGAAGATCCGTTTGAAGAGAACTCTTTTGATATGT
TTAGCCAACTACAAAATAAGCTGAGTTCAACAATAATTACTGGAGATGACCTATATACTACAAATATAAAATATCTAAAA
ATAGGTATAGAAAAGAGATCGACTAAGGGTGTTATAGTTAAGCCTAATCAAGTCGGTACAATATCTGAGACGTTTGAATT
TACTAATTTGGCTAGGAGAAACTCAATGAAGTTAATAACAAGTCATAGAAGTGGAGAGACTGAGGACAATTTCATAGCAG
ACTTTGCGGTGGGAATTGAGTCAGATTTCATAAAGGTTGGTGCACCGGCGAGAGGAGAGAGAACTAGCAAATATAATAAG
CTATTAGAAATAGAAAATAAATTTGGATTAGAATACGAAGGAAAATATTTTTATCTTTAA

Upstream 100 bases:

>100_bases
ATCTAGAGTAAATAGGAGAAAGTAAACCATATGCTAAAAATACTATTGAGGCCATACTAGGGGCTAACATTTAATTATTG
GTAGTTCATCAACAGTAAAT

Downstream 100 bases:

>100_bases
GGTAATAATATGCCTAAGATCACGAGGAAAATTGCTATCCCCAGTAAGAACAATATGAACTCCTTCGTTGACATTGATGG
AACAGCTCTAGCTGCCTTCG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MINRFSIEKVKGLEIVDSRGNPTIRVFIRTSDGVESFGDAPAGASKGTREAVEVRDENGLTVKRAVDIVNYIIDPALHGI
DVREQGIIDKLLKDIDSTENKSKLGGNTIIATSIAALKTASKALGLEVFKYISGPRLPKIPIPLLNIINGGLHAGNKLKI
QEFIIVPIKFNTFKEALFAAIDVYRTLKGLITERYGKIYTAVGDEGGFSPPLEDTREALDLIYTSINNAGYEGKIYMGMD
AAGSDFYDSKKEKYIIDGRELDPNQLLEFYLDLVKQYPIVYLEDPFEENSFDMFSQLQNKLSSTIITGDDLYTTNIKYLK
IGIEKRSTKGVIVKPNQVGTISETFEFTNLARRNSMKLITSHRSGETEDNFIADFAVGIESDFIKVGAPARGERTSKYNK
LLEIENKFGLEYEGKYFYL

Sequences:

>Translated_419_residues
MINRFSIEKVKGLEIVDSRGNPTIRVFIRTSDGVESFGDAPAGASKGTREAVEVRDENGLTVKRAVDIVNYIIDPALHGI
DVREQGIIDKLLKDIDSTENKSKLGGNTIIATSIAALKTASKALGLEVFKYISGPRLPKIPIPLLNIINGGLHAGNKLKI
QEFIIVPIKFNTFKEALFAAIDVYRTLKGLITERYGKIYTAVGDEGGFSPPLEDTREALDLIYTSINNAGYEGKIYMGMD
AAGSDFYDSKKEKYIIDGRELDPNQLLEFYLDLVKQYPIVYLEDPFEENSFDMFSQLQNKLSSTIITGDDLYTTNIKYLK
IGIEKRSTKGVIVKPNQVGTISETFEFTNLARRNSMKLITSHRSGETEDNFIADFAVGIESDFIKVGAPARGERTSKYNK
LLEIENKFGLEYEGKYFYL
>Mature_419_residues
MINRFSIEKVKGLEIVDSRGNPTIRVFIRTSDGVESFGDAPAGASKGTREAVEVRDENGLTVKRAVDIVNYIIDPALHGI
DVREQGIIDKLLKDIDSTENKSKLGGNTIIATSIAALKTASKALGLEVFKYISGPRLPKIPIPLLNIINGGLHAGNKLKI
QEFIIVPIKFNTFKEALFAAIDVYRTLKGLITERYGKIYTAVGDEGGFSPPLEDTREALDLIYTSINNAGYEGKIYMGMD
AAGSDFYDSKKEKYIIDGRELDPNQLLEFYLDLVKQYPIVYLEDPFEENSFDMFSQLQNKLSSTIITGDDLYTTNIKYLK
IGIEKRSTKGVIVKPNQVGTISETFEFTNLARRNSMKLITSHRSGETEDNFIADFAVGIESDFIKVGAPARGERTSKYNK
LLEIENKFGLEYEGKYFYL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=425, Percent_Identity=42.5882352941177, Blast_Score=331, Evalue=7e-91,
Organism=Homo sapiens, GI4503571, Length=433, Percent_Identity=41.5704387990762, Blast_Score=328, Evalue=5e-90,
Organism=Homo sapiens, GI301897477, Length=424, Percent_Identity=41.0377358490566, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI301897469, Length=424, Percent_Identity=41.0377358490566, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI301897479, Length=417, Percent_Identity=38.1294964028777, Blast_Score=284, Evalue=9e-77,
Organism=Homo sapiens, GI169201331, Length=352, Percent_Identity=26.1363636363636, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI169201757, Length=352, Percent_Identity=26.1363636363636, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI239744207, Length=352, Percent_Identity=26.1363636363636, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789141, Length=418, Percent_Identity=42.1052631578947, Blast_Score=297, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI71995829, Length=436, Percent_Identity=43.1192660550459, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=43.287037037037, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI32563855, Length=206, Percent_Identity=39.8058252427184, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323985, Length=436, Percent_Identity=41.5137614678899, Blast_Score=306, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6324974, Length=436, Percent_Identity=41.2844036697248, Blast_Score=306, Evalue=6e-84,
Organism=Saccharomyces cerevisiae, GI6324969, Length=436, Percent_Identity=41.2844036697248, Blast_Score=306, Evalue=6e-84,
Organism=Saccharomyces cerevisiae, GI6321693, Length=426, Percent_Identity=41.5492957746479, Blast_Score=300, Evalue=4e-82,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=38.2151029748284, Blast_Score=282, Evalue=7e-77,
Organism=Drosophila melanogaster, GI24580918, Length=436, Percent_Identity=41.0550458715596, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24580916, Length=436, Percent_Identity=41.0550458715596, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24580920, Length=436, Percent_Identity=41.0550458715596, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24580914, Length=436, Percent_Identity=41.0550458715596, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=41.0138248847926, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=41.0138248847926, Blast_Score=312, Evalue=3e-85,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46746; Mature: 46746

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINRFSIEKVKGLEIVDSRGNPTIRVFIRTSDGVESFGDAPAGASKGTREAVEVRDENGL
CCCCCCCHHHCCEEEEECCCCCEEEEEEEECCCHHHHCCCCCCCCCCCCHHHEEECCCCC
TVKRAVDIVNYIIDPALHGIDVREQGIIDKLLKDIDSTENKSKLGGNTIIATSIAALKTA
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCEEEEHHHHHHHHH
SKALGLEVFKYISGPRLPKIPIPLLNIINGGLHAGNKLKIQEFIIVPIKFNTFKEALFAA
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEEEEEEEECCHHHHHHHHH
IDVYRTLKGLITERYGKIYTAVGDEGGFSPPLEDTREALDLIYTSINNAGYEGKIYMGMD
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECC
AAGSDFYDSKKEKYIIDGRELDPNQLLEFYLDLVKQYPIVYLEDPFEENSFDMFSQLQNK
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHH
LSSTIITGDDLYTTNIKYLKIGIEKRSTKGVIVKPNQVGTISETFEFTNLARRNSMKLIT
HHHEEECCCCEEECCEEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCCEEEEE
SHRSGETEDNFIADFAVGIESDFIKVGAPARGERTSKYNKLLEIENKFGLEYEGKYFYL
ECCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEECCCEEEC
>Mature Secondary Structure
MINRFSIEKVKGLEIVDSRGNPTIRVFIRTSDGVESFGDAPAGASKGTREAVEVRDENGL
CCCCCCCHHHCCEEEEECCCCCEEEEEEEECCCHHHHCCCCCCCCCCCCHHHEEECCCCC
TVKRAVDIVNYIIDPALHGIDVREQGIIDKLLKDIDSTENKSKLGGNTIIATSIAALKTA
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCEEEEHHHHHHHHH
SKALGLEVFKYISGPRLPKIPIPLLNIINGGLHAGNKLKIQEFIIVPIKFNTFKEALFAA
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEEEEEEEECCHHHHHHHHH
IDVYRTLKGLITERYGKIYTAVGDEGGFSPPLEDTREALDLIYTSINNAGYEGKIYMGMD
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECC
AAGSDFYDSKKEKYIIDGRELDPNQLLEFYLDLVKQYPIVYLEDPFEENSFDMFSQLQNK
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHH
LSSTIITGDDLYTTNIKYLKIGIEKRSTKGVIVKPNQVGTISETFEFTNLARRNSMKLIT
HHHEEECCCCEEECCEEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCCEEEEE
SHRSGETEDNFIADFAVGIESDFIKVGAPARGERTSKYNKLLEIENKFGLEYEGKYFYL
ECCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA