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The map label for this gene is rfbD-1 [H]

Identifier: 15897732

GI number: 15897732

Start: 713867

End: 714547

Strand: Reverse

Name: rfbD-1 [H]

Synonym: SSO0832

Alternate gene names: 15897732

Gene position: 714547-713867 (Counterclockwise)

Preceding gene: 15897733

Following gene: 15897731

Centisome position: 23.88

GC content: 41.26

Gene sequence:

>681_bases
TTGGAGGATTTCATAATTAAGACTAGGCCTGATGTTATAATAAACACTGCAGCTATGACTGACGTAGACAAGTGCGAGGT
AGAAAGAGAAAAGGCTTATAAAATAAATGCCGAAGCAGTAAAACACATGGTAAGAGCTAGTAGGGTAGTGGAAGCTTACT
TTATTCACGTGAGTACGGACTACGTTTTTGACGGCACGAAGGGGAATTACAAAGAGGATGACTTACCAAATCCAATAAAC
TACTATGGGCTGACAAAGTTATTAGGAGAGACCTTTGCCTTATCATACGACGACAGCCTCGTTATAAGGACTTCTGGGGT
ATTCAGACACAAGGGGTTTCCAGTATATGTGTACAAAACGTTAAAGGAAGGGAAGACAGTCTTCGCGTATAAAGGTTATT
ACTCGCCCATTTCAGCGAGGAAACTAGCATCAGCAATAGAAGAGTTACTGGCCCTTAGGAAAACTGGTTTACTAAATGTT
GCAGGAGAGAGAATATCGCGTTACGAGCTGGCTCTAAAGATTAAGGAAAAGTTCAATTTGCCAGGAGAAGTGAAGGAAGT
AGATGAGGTTAAAGGATGGACAGCTAGGAGGCCCTTTGACTCCTCTTTGGACTACTCAAAAGCTAAGAAAATCTTATCCG
TCGATTTTTACAGTTTAGACCTAGAAGGTATGGTGTTGTGA

Upstream 100 bases:

>100_bases
AATTAAATAAGTTACTTTCTAAAAAATACCAAGTGATCAAGGTGTATAACTCGTCTGAAATTCCTGACAGTCATAAAATG
GACATAACCGATTTTCCCGT

Downstream 100 bases:

>100_bases
GATGGAGGCAGTAATCCTACATGGAGGGCAAGGTACAAGGCTCAGGCCGTTAACCCATACTGGCCCAAAGCAGTTGATAA
AAGTCGCAGGAAAGCCGGTT

Product: dTDP-4-dehydrorhamnose reductase (rfbD-1)

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MEDFIIKTRPDVIINTAAMTDVDKCEVEREKAYKINAEAVKHMVRASRVVEAYFIHVSTDYVFDGTKGNYKEDDLPNPIN
YYGLTKLLGETFALSYDDSLVIRTSGVFRHKGFPVYVYKTLKEGKTVFAYKGYYSPISARKLASAIEELLALRKTGLLNV
AGERISRYELALKIKEKFNLPGEVKEVDEVKGWTARRPFDSSLDYSKAKKILSVDFYSLDLEGMVL

Sequences:

>Translated_226_residues
MEDFIIKTRPDVIINTAAMTDVDKCEVEREKAYKINAEAVKHMVRASRVVEAYFIHVSTDYVFDGTKGNYKEDDLPNPIN
YYGLTKLLGETFALSYDDSLVIRTSGVFRHKGFPVYVYKTLKEGKTVFAYKGYYSPISARKLASAIEELLALRKTGLLNV
AGERISRYELALKIKEKFNLPGEVKEVDEVKGWTARRPFDSSLDYSKAKKILSVDFYSLDLEGMVL
>Mature_226_residues
MEDFIIKTRPDVIINTAAMTDVDKCEVEREKAYKINAEAVKHMVRASRVVEAYFIHVSTDYVFDGTKGNYKEDDLPNPIN
YYGLTKLLGETFALSYDDSLVIRTSGVFRHKGFPVYVYKTLKEGKTVFAYKGYYSPISARKLASAIEELLALRKTGLLNV
AGERISRYELALKIKEKFNLPGEVKEVDEVKGWTARRPFDSSLDYSKAKKILSVDFYSLDLEGMVL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=220, Percent_Identity=29.5454545454545, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI33519455, Length=220, Percent_Identity=29.5454545454545, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788352, Length=220, Percent_Identity=35, Blast_Score=87, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 25727; Mature: 25727

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDFIIKTRPDVIINTAAMTDVDKCEVEREKAYKINAEAVKHMVRASRVVEAYFIHVSTD
CCCCEEECCCCEEEEEHHHCCCHHHHHHHHHHEEECHHHHHHHHHHHHHEEEEEEEEECC
YVFDGTKGNYKEDDLPNPINYYGLTKLLGETFALSYDDSLVIRTSGVFRHKGFPVYVYKT
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHEEEECCCEEEEEECCEEEECCCEEEEEEE
LKEGKTVFAYKGYYSPISARKLASAIEELLALRKTGLLNVAGERISRYELALKIKEKFNL
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEHHHCCC
PGEVKEVDEVKGWTARRPFDSSLDYSKAKKILSVDFYSLDLEGMVL
CCCHHHHHHHCCCCCCCCCCCCCCHHHHHHEEEEEEEEECCCEEEC
>Mature Secondary Structure
MEDFIIKTRPDVIINTAAMTDVDKCEVEREKAYKINAEAVKHMVRASRVVEAYFIHVSTD
CCCCEEECCCCEEEEEHHHCCCHHHHHHHHHHEEECHHHHHHHHHHHHHEEEEEEEEECC
YVFDGTKGNYKEDDLPNPINYYGLTKLLGETFALSYDDSLVIRTSGVFRHKGFPVYVYKT
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHEEEECCCEEEEEECCEEEECCCEEEEEEE
LKEGKTVFAYKGYYSPISARKLASAIEELLALRKTGLLNVAGERISRYELALKIKEKFNL
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEHHHCCC
PGEVKEVDEVKGWTARRPFDSSLDYSKAKKILSVDFYSLDLEGMVL
CCCHHHHHHHCCCCCCCCCCCCCCHHHHHHEEEEEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]