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The map label for this gene is rfbB-1 [H]

Identifier: 15897730

GI number: 15897730

Start: 711881

End: 712834

Strand: Reverse

Name: rfbB-1 [H]

Synonym: SSO0830

Alternate gene names: 15897730

Gene position: 712834-711881 (Counterclockwise)

Preceding gene: 15897731

Following gene: 15897727

Centisome position: 23.82

GC content: 42.77

Gene sequence:

>954_bases
ATGAAAATCATAGTAGTTGGTGGTGCTGGGTTCATAGGCTCCGCTTTCGTGAGGGAGTTGAACAAGAGGGGTATAAAGCC
CATCGTAGTAGACCTCTTAACCTACGCTGGAAGGAAGGAAAACCTCATTGGGACAGACTACGATTTCGTCCAAGCCGACG
TTAGGAGCGAAAAAATGCATGATATAATTAAGGAGTATTCTCCAGACATCGTCATAAATTTCGCAGCCGAGACCCACGTA
GATAGGTCAATTTATAAACCACAAGATTTCGTAACTACTAATGTTCTAGGTACGGTAAACTTACTTGAGGCTTCACGGAA
ATTCAATTTCAAATACGTCCACATCTCTACAGATGAGGTTTACGGTGAGGAGTGCGGGGATGAGAACTCACCTTTAAAAC
CCTCTTCTCCTTATAGCGCGTCTAAAGCGTCAGCTGACTTGTTCGTAAAGGCTTACGTAAGGACTTATGGGATAAGTGCG
GTTATAGTTAGACCATCTAATAACTACGGTCCTAGGCAGTTCCCTGAAAAGTTAATACCTAAGGCTATAATTAGGACTTT
ACTAGGGATACACATTCCAGTGTACGGTGATGGGAAAGCTGAAAGAGACTGGATATTTGTAGAGGACACTGCTAGGATAA
TTTTCGACGTGGTGAGTAGAGCTGAGTGGAAAGGGGAAGTATATAACATACCGGGAGGGCAGAGGTATAATGTGCTCGAG
ATCTTGAAGATGTTGGAGGAAGTGAGCGGTAAAGAGGTTAAGATAAAGTTTGTCTCAGATAGACCTGGCCACGATAGAAG
GTATTGTATGACAACAAGTATGAAATATGAAGTAACGCCATTTAAGGAGGGTCTGAGGAGGACTTATGAATGGTATTTGA
ATAACAGATGGTGGTGGGAGCCTTTAATTAATGATAAGTTCTTCGTAGAAGACGAGCCGTGGTTGACAGTTTAG

Upstream 100 bases:

>100_bases
GGGTGACACTAATGGACTCTTTAATAGGGAATAACTCTACTGTTGAAAAAGGTGGGAGATGGCAAAAGCTAATTATAGGT
GAGAACTCCTCGGTGATATT

Downstream 100 bases:

>100_bases
ACAAAGTTTTTATAAGATTACCTAGGTTAATTTTAGCCTTCACGATGGTTCCCTTAACTTTGCTTTCTATTCATTCTGCC
AATATGTTTCACATGCTCTC

Product: dTDP-glucose 4,6-dehydratase (rfbB-1)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKIIVVGGAGFIGSAFVRELNKRGIKPIVVDLLTYAGRKENLIGTDYDFVQADVRSEKMHDIIKEYSPDIVINFAAETHV
DRSIYKPQDFVTTNVLGTVNLLEASRKFNFKYVHISTDEVYGEECGDENSPLKPSSPYSASKASADLFVKAYVRTYGISA
VIVRPSNNYGPRQFPEKLIPKAIIRTLLGIHIPVYGDGKAERDWIFVEDTARIIFDVVSRAEWKGEVYNIPGGQRYNVLE
ILKMLEEVSGKEVKIKFVSDRPGHDRRYCMTTSMKYEVTPFKEGLRRTYEWYLNNRWWWEPLINDKFFVEDEPWLTV

Sequences:

>Translated_317_residues
MKIIVVGGAGFIGSAFVRELNKRGIKPIVVDLLTYAGRKENLIGTDYDFVQADVRSEKMHDIIKEYSPDIVINFAAETHV
DRSIYKPQDFVTTNVLGTVNLLEASRKFNFKYVHISTDEVYGEECGDENSPLKPSSPYSASKASADLFVKAYVRTYGISA
VIVRPSNNYGPRQFPEKLIPKAIIRTLLGIHIPVYGDGKAERDWIFVEDTARIIFDVVSRAEWKGEVYNIPGGQRYNVLE
ILKMLEEVSGKEVKIKFVSDRPGHDRRYCMTTSMKYEVTPFKEGLRRTYEWYLNNRWWWEPLINDKFFVEDEPWLTV
>Mature_317_residues
MKIIVVGGAGFIGSAFVRELNKRGIKPIVVDLLTYAGRKENLIGTDYDFVQADVRSEKMHDIIKEYSPDIVINFAAETHV
DRSIYKPQDFVTTNVLGTVNLLEASRKFNFKYVHISTDEVYGEECGDENSPLKPSSPYSASKASADLFVKAYVRTYGISA
VIVRPSNNYGPRQFPEKLIPKAIIRTLLGIHIPVYGDGKAERDWIFVEDTARIIFDVVSRAEWKGEVYNIPGGQRYNVLE
ILKMLEEVSGKEVKIKFVSDRPGHDRRYCMTTSMKYEVTPFKEGLRRTYEWYLNNRWWWEPLINDKFFVEDEPWLTV

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=322, Percent_Identity=33.8509316770186, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI56237023, Length=304, Percent_Identity=29.2763157894737, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI56118217, Length=304, Percent_Identity=29.2763157894737, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI189083684, Length=304, Percent_Identity=29.2763157894737, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI42516563, Length=327, Percent_Identity=24.4648318042813, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=44.7368421052632, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI48994969, Length=346, Percent_Identity=42.7745664739884, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1786974, Length=339, Percent_Identity=28.023598820059, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788366, Length=207, Percent_Identity=28.5024154589372, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=322, Percent_Identity=35.0931677018634, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI115532424, Length=316, Percent_Identity=29.746835443038, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71982035, Length=312, Percent_Identity=28.525641025641, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=314, Percent_Identity=28.343949044586, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=225, Percent_Identity=28.8888888888889, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=308, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19923002, Length=301, Percent_Identity=29.9003322259136, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21356223, Length=316, Percent_Identity=26.8987341772152, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36487; Mature: 36487

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIVVGGAGFIGSAFVRELNKRGIKPIVVDLLTYAGRKENLIGTDYDFVQADVRSEKMH
CEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
DIIKEYSPDIVINFAAETHVDRSIYKPQDFVTTNVLGTVNLLEASRKFNFKYVHISTDEV
HHHHHCCCCEEEEEEHHHCCCHHHCCCCHHEEHHHHHHHHHHHHHCCCCEEEEEEECHHH
YGEECGDENSPLKPSSPYSASKASADLFVKAYVRTYGISAVIVRPSNNYGPRQFPEKLIP
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHH
KAIIRTLLGIHIPVYGDGKAERDWIFVEDTARIIFDVVSRAEWKGEVYNIPGGQRYNVLE
HHHHHHHHHCEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHH
ILKMLEEVSGKEVKIKFVSDRPGHDRRYCMTTSMKYEVTPFKEGLRRTYEWYLNNRWWWE
HHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCEEEECHHHHHHHHHHHHHCCCEEEEC
PLINDKFFVEDEPWLTV
CCCCCEEEECCCCCCCC
>Mature Secondary Structure
MKIIVVGGAGFIGSAFVRELNKRGIKPIVVDLLTYAGRKENLIGTDYDFVQADVRSEKMH
CEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
DIIKEYSPDIVINFAAETHVDRSIYKPQDFVTTNVLGTVNLLEASRKFNFKYVHISTDEV
HHHHHCCCCEEEEEEHHHCCCHHHCCCCHHEEHHHHHHHHHHHHHCCCCEEEEEEECHHH
YGEECGDENSPLKPSSPYSASKASADLFVKAYVRTYGISAVIVRPSNNYGPRQFPEKLIP
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHH
KAIIRTLLGIHIPVYGDGKAERDWIFVEDTARIIFDVVSRAEWKGEVYNIPGGQRYNVLE
HHHHHHHHHCEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHH
ILKMLEEVSGKEVKIKFVSDRPGHDRRYCMTTSMKYEVTPFKEGLRRTYEWYLNNRWWWE
HHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCEEEECHHHHHHHHHHHHHCCCEEEEC
PLINDKFFVEDEPWLTV
CCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]