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The map label for this gene is lpdA [H]

Identifier: 15889887

GI number: 15889887

Start: 2615578

End: 2616984

Strand: Reverse

Name: lpdA [H]

Synonym: Atu2632

Alternate gene names: 15889887

Gene position: 2616984-2615578 (Counterclockwise)

Preceding gene: 159185302

Following gene: 159185301

Centisome position: 92.1

GC content: 59.06

Gene sequence:

>1407_bases
ATGGCATATGATGTAGTTGTAATCGGAACGGGTCCCGGTGGTTATGTTTGCGCGGTCAAGGCGGCACAGCTCGGCCTCAA
GGTCGCAGTGATCGAAAAGCGCGCGACCTATGGCGGCACCTGCCTCAATGTCGGCTGCATTCCTTCCAAGGCGTTGCTGC
ACGCCTCCGAAACCTTCGCGCATGTGGCCCACGGCGTTGATACGCTTGGTATCGAAGTCGCCGCTCCGAAGCTGAACCTC
GAGAAGATGATGGGGCACAAAGACGGCGTGGTGAAAGCCAATGTCGACGGCGTTGCTTTCCTGTTCAAGAAGAACAAGAT
CGATGCTTTCCAGGGCACTGGCAAGGTGGTTTCCGCCGGCAAGGTTTCCGTCACCAACGACAAGGGTGAGACCCAGGAGA
TCGAAGCCAAGAACATCGTCATCGCCACCGGTTCCGATGTTGCCGGCATTCCGGGTGTCAAGGTCGATATCGATGAAACC
GTCATCGTGTCCTCCACCGGTGCGATTGCTCTTTCCAAGGTTCCGGAAAAACTCATCGTCGTTGGCGGTGGTGTCATCGG
TCTCGAGCTTGGTTCGGTCTGGTCGCGTCTTGGCGCGAAGGTGACCGTCGTCGAATATCTCGACAACATTCTCGGTGGCA
TGGATGGCGAAGTTTCCAAGCAGTCGCAGCGTCTGCTCGCCAAGCAGGGTCTCGATTTCAAGCTTGGCGCCAAGGTGACG
GCCGTTGAAAAGACCGCTGCCGGTGCAAAGGTCGTATTCGAGCCGGTCAAGGGCGGCGCGGCTGAAACGCTGGAAGCTGA
TGTCGTGCTGATCTCCACGGGGCGCAAGCCCTACACCGAAGGTCTCGGCCTTGCGGAAGCCGGTGTTGTGCTCGACAGCC
GCGGGCGTGTCGAGATCGACGGCCACTACAAGACCAATGTCGATGGCATCTATGCGATCGGCGATGTGGTGAAGGGCCCG
ATGCTGGCACACAAGGCGGAAGATGAGGGCGTTGCGCTTGCTGAAATCCTCGCCGGCCAGCGTGGCCATGTGAACTACGA
CGTCATTCCGGCCGTCGTTTATACGCAGCCGGAAATCGCTTCCGTCGGCAATACAGAAGAGGAACTGAAGGCCGCTGGCG
TCGCCTACAAGGTCGGTAAGTTCCCCTTCACCGCCAATGGCCGCGCCCGCGCCATGCAGGTGACGGATGGTTTCGTGAAA
ATCCTTGCCGACAAGGAAACCGACCGGGTTCTCGGCGGCCATATCGTCGGCTTCGGCGCCGGCGAAATGATCCACGAGAT
CACCGTGCTGATGGAATTCGGCGGTTCTTCAGAGGATCTTGGCCGCACCTGCCACGCGCACCCGACCATGTCGGAAGCCG
TGAAGGAAGCAGCACTTGCCACCTTCTTCAAGCCGATCCACATGTGA

Upstream 100 bases:

>100_bases
ACTCGGCATAAAACTCGCAACGGAAAAAGCGGCCTGAGTTTCAGGTCAAACGGAAGCAGGGCGGTTCAAATCCTGCCCGA
TATCAATCAGGAACAGAAAT

Downstream 100 bases:

>100_bases
TCGGCATTAAGCCTGACGTTCGAATATTAAATTTCAGTCATCTGTTGAACCCCGGCTTGCCATGCAGGCCGGGGTTTCTC
ATACTCGTGCCATAGCCTGT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MAYDVVVIGTGPGGYVCAVKAAQLGLKVAVIEKRATYGGTCLNVGCIPSKALLHASETFAHVAHGVDTLGIEVAAPKLNL
EKMMGHKDGVVKANVDGVAFLFKKNKIDAFQGTGKVVSAGKVSVTNDKGETQEIEAKNIVIATGSDVAGIPGVKVDIDET
VIVSSTGAIALSKVPEKLIVVGGGVIGLELGSVWSRLGAKVTVVEYLDNILGGMDGEVSKQSQRLLAKQGLDFKLGAKVT
AVEKTAAGAKVVFEPVKGGAAETLEADVVLISTGRKPYTEGLGLAEAGVVLDSRGRVEIDGHYKTNVDGIYAIGDVVKGP
MLAHKAEDEGVALAEILAGQRGHVNYDVIPAVVYTQPEIASVGNTEEELKAAGVAYKVGKFPFTANGRARAMQVTDGFVK
ILADKETDRVLGGHIVGFGAGEMIHEITVLMEFGGSSEDLGRTCHAHPTMSEAVKEAALATFFKPIHM

Sequences:

>Translated_468_residues
MAYDVVVIGTGPGGYVCAVKAAQLGLKVAVIEKRATYGGTCLNVGCIPSKALLHASETFAHVAHGVDTLGIEVAAPKLNL
EKMMGHKDGVVKANVDGVAFLFKKNKIDAFQGTGKVVSAGKVSVTNDKGETQEIEAKNIVIATGSDVAGIPGVKVDIDET
VIVSSTGAIALSKVPEKLIVVGGGVIGLELGSVWSRLGAKVTVVEYLDNILGGMDGEVSKQSQRLLAKQGLDFKLGAKVT
AVEKTAAGAKVVFEPVKGGAAETLEADVVLISTGRKPYTEGLGLAEAGVVLDSRGRVEIDGHYKTNVDGIYAIGDVVKGP
MLAHKAEDEGVALAEILAGQRGHVNYDVIPAVVYTQPEIASVGNTEEELKAAGVAYKVGKFPFTANGRARAMQVTDGFVK
ILADKETDRVLGGHIVGFGAGEMIHEITVLMEFGGSSEDLGRTCHAHPTMSEAVKEAALATFFKPIHM
>Mature_467_residues
AYDVVVIGTGPGGYVCAVKAAQLGLKVAVIEKRATYGGTCLNVGCIPSKALLHASETFAHVAHGVDTLGIEVAAPKLNLE
KMMGHKDGVVKANVDGVAFLFKKNKIDAFQGTGKVVSAGKVSVTNDKGETQEIEAKNIVIATGSDVAGIPGVKVDIDETV
IVSSTGAIALSKVPEKLIVVGGGVIGLELGSVWSRLGAKVTVVEYLDNILGGMDGEVSKQSQRLLAKQGLDFKLGAKVTA
VEKTAAGAKVVFEPVKGGAAETLEADVVLISTGRKPYTEGLGLAEAGVVLDSRGRVEIDGHYKTNVDGIYAIGDVVKGPM
LAHKAEDEGVALAEILAGQRGHVNYDVIPAVVYTQPEIASVGNTEEELKAAGVAYKVGKFPFTANGRARAMQVTDGFVKI
LADKETDRVLGGHIVGFGAGEMIHEITVLMEFGGSSEDLGRTCHAHPTMSEAVKEAALATFFKPIHM

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=464, Percent_Identity=56.0344827586207, Blast_Score=509, Evalue=1e-144,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=29.4243070362473, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI22035672, Length=464, Percent_Identity=30.8189655172414, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI291045266, Length=478, Percent_Identity=29.4979079497908, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI33519430, Length=474, Percent_Identity=27.6371308016878, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519428, Length=474, Percent_Identity=27.6371308016878, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519426, Length=474, Percent_Identity=27.6371308016878, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI148277065, Length=474, Percent_Identity=27.6371308016878, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI148277071, Length=474, Percent_Identity=27.6371308016878, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI291045268, Length=470, Percent_Identity=28.2978723404255, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1786307, Length=454, Percent_Identity=41.1894273127753, Blast_Score=327, Evalue=9e-91,
Organism=Escherichia coli, GI87082354, Length=464, Percent_Identity=31.8965517241379, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI87081717, Length=467, Percent_Identity=30.1927194860814, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1789915, Length=439, Percent_Identity=30.751708428246, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1789065, Length=191, Percent_Identity=27.7486910994764, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=460, Percent_Identity=54.1304347826087, Blast_Score=490, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17557007, Length=473, Percent_Identity=30.6553911205074, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI71982272, Length=486, Percent_Identity=27.3662551440329, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=448, Percent_Identity=26.1160714285714, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71983429, Length=448, Percent_Identity=26.1160714285714, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=257, Percent_Identity=25.6809338521401, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=50.7368421052632, Blast_Score=458, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=32.76955602537, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6325166, Length=470, Percent_Identity=28.7234042553192, Blast_Score=159, Evalue=9e-40,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=55.6503198294243, Blast_Score=521, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24640549, Length=483, Percent_Identity=29.399585921325, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640553, Length=483, Percent_Identity=29.399585921325, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640551, Length=483, Percent_Identity=29.399585921325, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17737741, Length=478, Percent_Identity=27.6150627615063, Blast_Score=113, Evalue=4e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48668; Mature: 48536

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYDVVVIGTGPGGYVCAVKAAQLGLKVAVIEKRATYGGTCLNVGCIPSKALLHASETFA
CCEEEEEEECCCCCEEEEEEHHHCCEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHH
HVAHGVDTLGIEVAAPKLNLEKMMGHKDGVVKANVDGVAFLFKKNKIDAFQGTGKVVSAG
HHHCCCCCCCEEEECCCCCHHHHCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCEEECC
KVSVTNDKGETQEIEAKNIVIATGSDVAGIPGVKVDIDETVIVSSTGAIALSKVPEKLIV
EEEEECCCCCCEEECCCEEEEECCCCCCCCCCEEEECCCEEEEECCCCEEEECCCCEEEE
VGGGVIGLELGSVWSRLGAKVTVVEYLDNILGGMDGEVSKQSQRLLAKQGLDFKLGAKVT
EECCEEEEEHHHHHHHHCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCEEE
AVEKTAAGAKVVFEPVKGGAAETLEADVVLISTGRKPYTEGLGLAEAGVVLDSRGRVEID
EEECCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCHHCCCCCCCCEEEECCCCEEEC
GHYKTNVDGIYAIGDVVKGPMLAHKAEDEGVALAEILAGQRGHVNYDVIPAVVYTQPEIA
CCEECCCCCEEEEHHHHCCCHHEECCCCCCCCHHHHHCCCCCCCCEEEEEEEEECCCCHH
SVGNTEEELKAAGVAYKVGKFPFTANGRARAMQVTDGFVKILADKETDRVLGGHIVGFGA
CCCCCHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCEEEEEECCCCCCEECCEEEEECC
GEMIHEITVLMEFGGSSEDLGRTCHAHPTMSEAVKEAALATFFKPIHM
HHHHHHHHEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AYDVVVIGTGPGGYVCAVKAAQLGLKVAVIEKRATYGGTCLNVGCIPSKALLHASETFA
CEEEEEEECCCCCEEEEEEHHHCCEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHH
HVAHGVDTLGIEVAAPKLNLEKMMGHKDGVVKANVDGVAFLFKKNKIDAFQGTGKVVSAG
HHHCCCCCCCEEEECCCCCHHHHCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCEEECC
KVSVTNDKGETQEIEAKNIVIATGSDVAGIPGVKVDIDETVIVSSTGAIALSKVPEKLIV
EEEEECCCCCCEEECCCEEEEECCCCCCCCCCEEEECCCEEEEECCCCEEEECCCCEEEE
VGGGVIGLELGSVWSRLGAKVTVVEYLDNILGGMDGEVSKQSQRLLAKQGLDFKLGAKVT
EECCEEEEEHHHHHHHHCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCEEE
AVEKTAAGAKVVFEPVKGGAAETLEADVVLISTGRKPYTEGLGLAEAGVVLDSRGRVEID
EEECCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCHHCCCCCCCCEEEECCCCEEEC
GHYKTNVDGIYAIGDVVKGPMLAHKAEDEGVALAEILAGQRGHVNYDVIPAVVYTQPEIA
CCEECCCCCEEEEHHHHCCCHHEECCCCCCCCHHHHHCCCCCCCCEEEEEEEEECCCCHH
SVGNTEEELKAAGVAYKVGKFPFTANGRARAMQVTDGFVKILADKETDRVLGGHIVGFGA
CCCCCHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCEEEEEECCCCCCEECCEEEEECC
GEMIHEITVLMEFGGSSEDLGRTCHAHPTMSEAVKEAALATFFKPIHM
HHHHHHHHEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9226266 [H]