Click here to switch to the map view.

The map label for this gene is stcD [H]

Identifier: 15889803

GI number: 15889803

Start: 2510197

End: 2512233

Strand: Direct

Name: stcD [H]

Synonym: Atu2538

Alternate gene names: 15889803

Gene position: 2510197-2512233 (Clockwise)

Preceding gene: 159185256

Following gene: 159185261

Centisome position: 88.34

GC content: 61.9

Gene sequence:

>2037_bases
ATGTCGAATGATCCCCTTCTTCAGCCCTACCAGCTGAAACACCTCACCCTGCGAAACCGCATCATCGTCACCTCGCATGA
GCCCGCCTATCCCGAGGATGGCATGCCGAAGGGGCGCTACCGCGCCTATACGGTGGAGCGGGCAAAAGGCGGTGTCGCCT
TGACGATGACGGCGGGCTCCGCCGCCGTCTCCCGGGACAGCCCGCCCGTCTTCAACAATCTGCTCGCCTACAAGGATGAA
ATCGTTCCCTGGATCAGGGAAATGACCGACGCCGTGCATGAACAGGGTGCGGCAATCATGATCCAGCTGACCCATCTCGG
CCGTCGCACCCGCTGGGACAAGGGCGACTGGCTGCCGGTGCTCGCCCCCTCCCACCAGCGGGAGGCGGCACACCGCGCCT
TCCCGAAGAAGATCGAGGATTGGGATATCGAGCGCGTCATCAAGGACTTCGCCGATGCCGCGGAACGCATGAAGGCAGGC
GGCATGGACGGAGTGGAGCTGGAGGCCTATGGCCATCTCATCGACCAGTTCGTCTCCCCGCTGACCAACGAGCTTGACGG
ACCCTATGGCGGCTCGCTGGACAACCGCATGCGCTTCTGTCTCGACGTCTTCAAGGCGATGCGTGAGCGTGTGGGCGATG
ATTTCATTCTTGGGGTCCGTTATACCGCCGACGAATGTCTTAAGGGCGGCACGGGCAAGGCAGAGGGTCTCGAAATCTCC
AGACGGCTGAGGGACAGCGGCCTGATCGACTATCTCAATGTCATCAGGGGCCATATCGATACCGATCCTGGCCTGACCGA
CGTCATTCCCATTCAGGGCATGGCCAATGCGCCGCATCTCGATTTTGCCGGCGAAATCCGCGCGGCAACCCAATTCCCGA
CCTTCCATGCCGCCAAAATCCAAGACGTCGCGACCGCCCGCCATGCGATCGCCTCCGGCAAGGTGGACATGGTCGGCATG
ACGCGCGCACACATGACCGACCCGCATATCGTGCGCAAGATCATGGAAAAACGGGAGGACGATATTCGCCCCTGCGTCGG
TGCCAATTATTGTCTCGACCGCATCTACCAGGGCGGCCTCGCCTTCTGCATCCACAATGCGGCGACCGGCCGCGAAGAAA
CCATGCCGCACGACATCACCAGAGCAGCGGAACGCCGCAAGGTGGTCATCGTCGGCGCCGGTCCGGCCGGGCTTGAGGCA
GCGCGTGTCTGCGCCGAACGCGGCCATGACGTTGTCGTCTTCGAGGCTGCGAACGATGCCGGCGGCCAGATACGCCTAAC
CGCTCAAAGCGAGCGCCGCCGCGAAATGATTGGCATTATCGACTGGCGCATGAGCCAATGCGAAAGGCTCGGCGTCACCT
TCCACTTCAACAGCTGGGCGGAAGCCGACACGGTCCTTTCGGAAAACCCCGATGTCGTCATCATCGCCACCGGCGGATTG
CCGCATACCGACGTGCTTTCAAAGGGCAACGATCTCGTCGTTTCCGCCTGGGATATCATTTCCGGTGACGTCAAGCCGGG
CACGAATGTGCTGGTCTTCGACGATGCCGGCGATCATGCCGGCCTGCAGGCCGCCGAATGTCTGGCAAAGGCAGGCGCGA
AGGTTGAGATCATGACGCCGGACCGCGCCTTCGCCCCCGAGGTCATGGCCATGAACCTGGTCCCCTATATGCGCGCGCTG
CAGAAACTCGATGTCACCTTCACCGTTACCTACCGGCTGGAAGCGGCGGAAAGAAACAGCAACCAGCTCATCGCCCATGT
CGGCAGCGACTATGGCGGCGTGGCGAAATCAAGGAATGTGGACCAGATCGTCGTCAATCACGGCACGGTGCCGCTGGACG
AGCTCTATTTCGAGCTGAAATCCGGCTCGAAAAACCTCGGCGAAACCTCCTACGACGCCCTGCTTGCGGGAGAGGCCCAG
ACAGTCGAACGCAACCCGGCCGGCACATACAGGCTCTTCCGGATCGGCGATGCCGTGGCCGCACGCAACACCCATGCGGC
GATCTACGATGCGCTGCGTCTGGCAAAGGACATCTGA

Upstream 100 bases:

>100_bases
CCAGCTATGTCGAAGCAATAAACACCTGTGTTTTCTTAATTGACACATATGTACATTCCCGCTTAGCCTCCTCCCATGAA
ACGTCTTTGGAGCATGGCGC

Downstream 100 bases:

>100_bases
GACAGATGTCGCGTCGGGAGGGCGAATTCGACCAGAGGGTATTCCAGCGTAAACGCAAAAAAACCCGCAGCTTGAGCTAC
GGGTTTTTTTGTATTTTGGT

Product: N-methylproline demethylase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MSNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKGRYRAYTVERAKGGVALTMTAGSAAVSRDSPPVFNNLLAYKDE
IVPWIREMTDAVHEQGAAIMIQLTHLGRRTRWDKGDWLPVLAPSHQREAAHRAFPKKIEDWDIERVIKDFADAAERMKAG
GMDGVELEAYGHLIDQFVSPLTNELDGPYGGSLDNRMRFCLDVFKAMRERVGDDFILGVRYTADECLKGGTGKAEGLEIS
RRLRDSGLIDYLNVIRGHIDTDPGLTDVIPIQGMANAPHLDFAGEIRAATQFPTFHAAKIQDVATARHAIASGKVDMVGM
TRAHMTDPHIVRKIMEKREDDIRPCVGANYCLDRIYQGGLAFCIHNAATGREETMPHDITRAAERRKVVIVGAGPAGLEA
ARVCAERGHDVVVFEAANDAGGQIRLTAQSERRREMIGIIDWRMSQCERLGVTFHFNSWAEADTVLSENPDVVIIATGGL
PHTDVLSKGNDLVVSAWDIISGDVKPGTNVLVFDDAGDHAGLQAAECLAKAGAKVEIMTPDRAFAPEVMAMNLVPYMRAL
QKLDVTFTVTYRLEAAERNSNQLIAHVGSDYGGVAKSRNVDQIVVNHGTVPLDELYFELKSGSKNLGETSYDALLAGEAQ
TVERNPAGTYRLFRIGDAVAARNTHAAIYDALRLAKDI

Sequences:

>Translated_678_residues
MSNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKGRYRAYTVERAKGGVALTMTAGSAAVSRDSPPVFNNLLAYKDE
IVPWIREMTDAVHEQGAAIMIQLTHLGRRTRWDKGDWLPVLAPSHQREAAHRAFPKKIEDWDIERVIKDFADAAERMKAG
GMDGVELEAYGHLIDQFVSPLTNELDGPYGGSLDNRMRFCLDVFKAMRERVGDDFILGVRYTADECLKGGTGKAEGLEIS
RRLRDSGLIDYLNVIRGHIDTDPGLTDVIPIQGMANAPHLDFAGEIRAATQFPTFHAAKIQDVATARHAIASGKVDMVGM
TRAHMTDPHIVRKIMEKREDDIRPCVGANYCLDRIYQGGLAFCIHNAATGREETMPHDITRAAERRKVVIVGAGPAGLEA
ARVCAERGHDVVVFEAANDAGGQIRLTAQSERRREMIGIIDWRMSQCERLGVTFHFNSWAEADTVLSENPDVVIIATGGL
PHTDVLSKGNDLVVSAWDIISGDVKPGTNVLVFDDAGDHAGLQAAECLAKAGAKVEIMTPDRAFAPEVMAMNLVPYMRAL
QKLDVTFTVTYRLEAAERNSNQLIAHVGSDYGGVAKSRNVDQIVVNHGTVPLDELYFELKSGSKNLGETSYDALLAGEAQ
TVERNPAGTYRLFRIGDAVAARNTHAAIYDALRLAKDI
>Mature_677_residues
SNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKGRYRAYTVERAKGGVALTMTAGSAAVSRDSPPVFNNLLAYKDEI
VPWIREMTDAVHEQGAAIMIQLTHLGRRTRWDKGDWLPVLAPSHQREAAHRAFPKKIEDWDIERVIKDFADAAERMKAGG
MDGVELEAYGHLIDQFVSPLTNELDGPYGGSLDNRMRFCLDVFKAMRERVGDDFILGVRYTADECLKGGTGKAEGLEISR
RLRDSGLIDYLNVIRGHIDTDPGLTDVIPIQGMANAPHLDFAGEIRAATQFPTFHAAKIQDVATARHAIASGKVDMVGMT
RAHMTDPHIVRKIMEKREDDIRPCVGANYCLDRIYQGGLAFCIHNAATGREETMPHDITRAAERRKVVIVGAGPAGLEAA
RVCAERGHDVVVFEAANDAGGQIRLTAQSERRREMIGIIDWRMSQCERLGVTFHFNSWAEADTVLSENPDVVIIATGGLP
HTDVLSKGNDLVVSAWDIISGDVKPGTNVLVFDDAGDHAGLQAAECLAKAGAKVEIMTPDRAFAPEVMAMNLVPYMRALQ
KLDVTFTVTYRLEAAERNSNQLIAHVGSDYGGVAKSRNVDQIVVNHGTVPLDELYFELKSGSKNLGETSYDALLAGEAQT
VERNPAGTYRLFRIGDAVAARNTHAAIYDALRLAKDI

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=542, Percent_Identity=29.7047970479705, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1787939, Length=367, Percent_Identity=26.9754768392371, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17565138, Length=307, Percent_Identity=27.6872964169381, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=271, Percent_Identity=26.1992619926199, Blast_Score=80, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17559804, Length=264, Percent_Identity=26.8939393939394, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17564188, Length=264, Percent_Identity=24.6212121212121, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI72001454, Length=261, Percent_Identity=25.6704980842912, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17566914, Length=180, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=357, Percent_Identity=23.8095238095238, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321973, Length=325, Percent_Identity=24.6153846153846, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 74496; Mature: 74364

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKGRYRAYTVERAKGGVALTMTAGS
CCCCCCCCCHHHHEEEEECEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCC
AAVSRDSPPVFNNLLAYKDEIVPWIREMTDAVHEQGAAIMIQLTHLGRRTRWDKGDWLPV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEE
LAPSHQREAAHRAFPKKIEDWDIERVIKDFADAAERMKAGGMDGVELEAYGHLIDQFVSP
ECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
LTNELDGPYGGSLDNRMRFCLDVFKAMRERVGDDFILGVRYTADECLKGGTGKAEGLEIS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHCCCCCCCCCHHHH
RRLRDSGLIDYLNVIRGHIDTDPGLTDVIPIQGMANAPHLDFAGEIRAATQFPTFHAAKI
HHHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHCCCCCCCHHHH
QDVATARHAIASGKVDMVGMTRAHMTDPHIVRKIMEKREDDIRPCVGANYCLDRIYQGGL
HHHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
AFCIHNAATGREETMPHDITRAAERRKVVIVGAGPAGLEAARVCAERGHDVVVFEAANDA
EEEEECCCCCCCCCCCHHHHHHHHCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCC
GGQIRLTAQSERRREMIGIIDWRMSQCERLGVTFHFNSWAEADTVLSENPDVVIIATGGL
CCEEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCCEEEEECCCC
PHTDVLSKGNDLVVSAWDIISGDVKPGTNVLVFDDAGDHAGLQAAECLAKAGAKVEIMTP
CCHHHHCCCCCEEEEEEHEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECC
DRAFAPEVMAMNLVPYMRALQKLDVTFTVTYRLEAAERNSNQLIAHVGSDYGGVAKSRNV
CCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCC
DQIVVNHGTVPLDELYFELKSGSKNLGETSYDALLAGEAQTVERNPAGTYRLFRIGDAVA
CEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHEECCCCHHCCCCCCCCEEEEEECCHHH
ARNTHAAIYDALRLAKDI
CCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKGRYRAYTVERAKGGVALTMTAGS
CCCCCCCCHHHHEEEEECEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCC
AAVSRDSPPVFNNLLAYKDEIVPWIREMTDAVHEQGAAIMIQLTHLGRRTRWDKGDWLPV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEE
LAPSHQREAAHRAFPKKIEDWDIERVIKDFADAAERMKAGGMDGVELEAYGHLIDQFVSP
ECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
LTNELDGPYGGSLDNRMRFCLDVFKAMRERVGDDFILGVRYTADECLKGGTGKAEGLEIS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHCCCCCCCCCHHHH
RRLRDSGLIDYLNVIRGHIDTDPGLTDVIPIQGMANAPHLDFAGEIRAATQFPTFHAAKI
HHHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHCCCCCCCHHHH
QDVATARHAIASGKVDMVGMTRAHMTDPHIVRKIMEKREDDIRPCVGANYCLDRIYQGGL
HHHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
AFCIHNAATGREETMPHDITRAAERRKVVIVGAGPAGLEAARVCAERGHDVVVFEAANDA
EEEEECCCCCCCCCCCHHHHHHHHCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCC
GGQIRLTAQSERRREMIGIIDWRMSQCERLGVTFHFNSWAEADTVLSENPDVVIIATGGL
CCEEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCCEEEEECCCC
PHTDVLSKGNDLVVSAWDIISGDVKPGTNVLVFDDAGDHAGLQAAECLAKAGAKVEIMTP
CCHHHHCCCCCEEEEEEHEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECC
DRAFAPEVMAMNLVPYMRALQKLDVTFTVTYRLEAAERNSNQLIAHVGSDYGGVAKSRNV
CCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCC
DQIVVNHGTVPLDELYFELKSGSKNLGETSYDALLAGEAQTVERNPAGTYRLFRIGDAVA
CEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHEECCCCHHCCCCCCCCEEEEEECCHHH
ARNTHAAIYDALRLAKDI
CCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]