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The map label for this gene is rutF

Identifier: 15889761

GI number: 15889761

Start: 2465843

End: 2466367

Strand: Reverse

Name: rutF

Synonym: Atu2495

Alternate gene names: 15889761

Gene position: 2466367-2465843 (Counterclockwise)

Preceding gene: 15889762

Following gene: 159185240

Centisome position: 86.8

GC content: 59.24

Gene sequence:

>525_bases
ATGCAGATGATGTCTCTCGAAAAGGAAGCGGCAATGGAGACGAAGACTGCTGAGCAGCGCAGCCTCGATTATAGAAACGC
CATGGCGCGGCTCGGTGCGGCGGTCAACATCGTCACGACGGATGGCGCGGCAGGCCGTGCGGGCTTTGCAGCGACGGCCG
TTTGCAGCGTTTCCGACAATCCGCCCACGCTTCTCGTCTGCCTCAACCGCAATGCCTCAGCCTACAAGGTGGTGAAGGCT
AATGGCGTTATCTGCATCAATACGCTTGCCGCCCATCACGAGGTGCTGAGCACGCTTTTTGGCGGCAAGACACCGGCGGA
AGAACGTTTCGCCGCCGGCAGCTGGGGCGTGCTCGAGACCGGCGCTCCGGTTCTGGAAGACGCACTGGTTTCTTTCGACT
GCCGTATCCGCGAGGCCCATGACGGCGGCACGCATGATATTCTGATCTGCACTGTCGTTGATATGAAGATCAACGCGGGT
GATGAAGCGCTGATGTATTTCAACCGGCGTTACCGGGTGCTTTGA

Upstream 100 bases:

>100_bases
CGAAGCTGTTCGGCCGCCTGCCGCGCCTTGGATTTGAAGACGCCTGTGTGCTGGCTTGATGTGTGCTGGCTTGATGGCCT
GTTAAGGAAGGATGGGTTAG

Downstream 100 bases:

>100_bases
ACGCAGTCTCTGGGGCCGGATGATACCGCTTAACTCACGATCAGCTACTTGCACACCATCATCCTTGGGCTTGTCCCGAG
GATTTGCAACCGATTAATTT

Product: flavoprotein oxidoreductase

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MQMMSLEKEAAMETKTAEQRSLDYRNAMARLGAAVNIVTTDGAAGRAGFAATAVCSVSDNPPTLLVCLNRNASAYKVVKA
NGVICINTLAAHHEVLSTLFGGKTPAEERFAAGSWGVLETGAPVLEDALVSFDCRIREAHDGGTHDILICTVVDMKINAG
DEALMYFNRRYRVL

Sequences:

>Translated_174_residues
MQMMSLEKEAAMETKTAEQRSLDYRNAMARLGAAVNIVTTDGAAGRAGFAATAVCSVSDNPPTLLVCLNRNASAYKVVKA
NGVICINTLAAHHEVLSTLFGGKTPAEERFAAGSWGVLETGAPVLEDALVSFDCRIREAHDGGTHDILICTVVDMKINAG
DEALMYFNRRYRVL
>Mature_174_residues
MQMMSLEKEAAMETKTAEQRSLDYRNAMARLGAAVNIVTTDGAAGRAGFAATAVCSVSDNPPTLLVCLNRNASAYKVVKA
NGVICINTLAAHHEVLSTLFGGKTPAEERFAAGSWGVLETGAPVLEDALVSFDCRIREAHDGGTHDILICTVVDMKINAG
DEALMYFNRRYRVL

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily

Homologues:

Organism=Escherichia coli, GI87081819, Length=151, Percent_Identity=56.953642384106, Blast_Score=180, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTF_AGRT5 (A9CHR3)

Other databases:

- EMBL:   AE007869
- PIR:   AD2883
- PIR:   B97659
- RefSeq:   NP_355442.1
- ProteinModelPortal:   A9CHR3
- SMR:   A9CHR3
- STRING:   A9CHR3
- GeneID:   1134533
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2495
- eggNOG:   COG1853
- HOGENOM:   HBG730363
- OMA:   NRSASVY
- PhylomeDB:   A9CHR3
- ProtClustDB:   CLSK2460689
- HAMAP:   MF_00833
- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- Gene3D:   G3DSA:2.30.110.10
- SMART:   SM00903
- TIGRFAMs:   TIGR03615

Pfam domain/function: PF01613 Flavin_Reduct; SSF50475 FMN_binding

EC number: =1.5.1.29

Molecular weight: Translated: 18667; Mature: 18667

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMMSLEKEAAMETKTAEQRSLDYRNAMARLGAAVNIVTTDGAAGRAGFAATAVCSVSDN
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCC
PPTLLVCLNRNASAYKVVKANGVICINTLAAHHEVLSTLFGGKTPAEERFAAGSWGVLET
CCEEEEEECCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCC
GAPVLEDALVSFDCRIREAHDGGTHDILICTVVDMKINAGDEALMYFNRRYRVL
CCHHHHHHHHHHCEEEEECCCCCCCEEEEEEEEEEEECCCCCEEEEEECEEECC
>Mature Secondary Structure
MQMMSLEKEAAMETKTAEQRSLDYRNAMARLGAAVNIVTTDGAAGRAGFAATAVCSVSDN
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCC
PPTLLVCLNRNASAYKVVKANGVICINTLAAHHEVLSTLFGGKTPAEERFAAGSWGVLET
CCEEEEEECCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCC
GAPVLEDALVSFDCRIREAHDGGTHDILICTVVDMKINAGDEALMYFNRRYRVL
CCHHHHHHHHHHCEEEEECCCCCCCEEEEEEEEEEEECCCCCEEEEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194