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The map label for this gene is lguL [C]

Identifier: 15889727

GI number: 15889727

Start: 2427145

End: 2427540

Strand: Reverse

Name: lguL [C]

Synonym: Atu2456

Alternate gene names: 15889727

Gene position: 2427540-2427145 (Counterclockwise)

Preceding gene: 15889729

Following gene: 159185223

Centisome position: 85.43

GC content: 56.06

Gene sequence:

>396_bases
ATGGCCAAGCTGATCCACTCGATGGTCCGGGTGCTGGACGAAAAGCGTTCGGTGGAATTTTACAAACAGGCTTTCGGGCT
TGAGATCGCGGAACGCGCCGAGTTCGAGAATTTCACGCTGATCTATCTCAGCAATGCCGAGGGCGATTTCGAGCTGGAAC
TGACCGTCAACCGGGCTCGCGAAGTGCCCTATGCGCTGGGCGACGGTTATGGCCATCTCGCCGTCAGTGTTGCCGACGTG
GACGCCGAACATCAGCGTTTCATCGGGATCGGGCTTACCCCCGGCAAGATGATCGAAGCCGATCATAAGGGCCAGCCTTT
CGCAAAATACTTTTTCATCTGCGATCCGGATGGTTACAAGATCGAAGTGCTGCAGCGCGGCGACCGGTTCAAATAA

Upstream 100 bases:

>100_bases
GCGCCTTTAGGGTTGAAAGCGCGGCGCTCAATCCTTATTCCCTTGGGCCGCGATGCCCGGAGCATAACCGGGCCGTCAAC
AGGAAGAAGAAGGAAACACC

Downstream 100 bases:

>100_bases
GCTCTGATGGAGGCGGGCATGGCAGAGGCAGGCGTCAAACTCGAAGACAGCTGGAAGCACGTTCTCTCGGGCGAATTCGC
CAGCCCCTACATGCAGAAGC

Product: lactoylglutathione lyase

Products: glutathione; methylglyoxal

Alternate protein names: Glyoxalase/Bleomycin Resistance Protein/Dioxygenase; Glyoxalase I; Glyoxalase Family Protein; Glyoxalase I/Lactoylglutathione Lyase; Glyoxalase; Lactoylglutathione Lyase-Like Protein; Lactoylglutathione Lyase Protein

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MAKLIHSMVRVLDEKRSVEFYKQAFGLEIAERAEFENFTLIYLSNAEGDFELELTVNRAREVPYALGDGYGHLAVSVADV
DAEHQRFIGIGLTPGKMIEADHKGQPFAKYFFICDPDGYKIEVLQRGDRFK

Sequences:

>Translated_131_residues
MAKLIHSMVRVLDEKRSVEFYKQAFGLEIAERAEFENFTLIYLSNAEGDFELELTVNRAREVPYALGDGYGHLAVSVADV
DAEHQRFIGIGLTPGKMIEADHKGQPFAKYFFICDPDGYKIEVLQRGDRFK
>Mature_130_residues
AKLIHSMVRVLDEKRSVEFYKQAFGLEIAERAEFENFTLIYLSNAEGDFELELTVNRAREVPYALGDGYGHLAVSVADVD
AEHQRFIGIGLTPGKMIEADHKGQPFAKYFFICDPDGYKIEVLQRGDRFK

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.4.1.5

Molecular weight: Translated: 14921; Mature: 14790

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLIHSMVRVLDEKRSVEFYKQAFGLEIAERAEFENFTLIYLSNAEGDFELELTVNRAR
CHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHCCCCCEEEEEEECCCCCEEEEEEEECCC
EVPYALGDGYGHLAVSVADVDAEHQRFIGIGLTPGKMIEADHKGQPFAKYFFICDPDGYK
CCCEEECCCCCEEEEEEECCCCCCCEEEEEECCCCCEEECCCCCCCCEEEEEEECCCCCE
IEVLQRGDRFK
EEEEECCCCCC
>Mature Secondary Structure 
AKLIHSMVRVLDEKRSVEFYKQAFGLEIAERAEFENFTLIYLSNAEGDFELELTVNRAR
HHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHCCCCCEEEEEEECCCCCEEEEEEEECCC
EVPYALGDGYGHLAVSVADVDAEHQRFIGIGLTPGKMIEADHKGQPFAKYFFICDPDGYK
CCCEEECCCCCEEEEEEECCCCCCCEEEEEECCCCCEEECCCCCCCCEEEEEEECCCCCE
IEVLQRGDRFK
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA