Click here to switch to the map view.

The map label for this gene is purA

Identifier: 15889718

GI number: 15889718

Start: 2418928

End: 2420226

Strand: Reverse

Name: purA

Synonym: Atu2447

Alternate gene names: 15889718

Gene position: 2420226-2418928 (Counterclockwise)

Preceding gene: 159185218

Following gene: 15889717

Centisome position: 85.17

GC content: 60.97

Gene sequence:

>1299_bases
ATGACGAATGTTGTGGTGGTCGGCTCGCAATGGGGCGACGAAGGCAAAGGCAAGATTGTCGACTGGCTGTCCGAGCGGGC
AGATGTCGTCGTGCGATATCAAGGCGGCCATAATGCCGGCCACACGCTTGTCATCGACGGCATCAGCTACAAGCTTTCGC
TTCTGCCGTCCGGCGTCGTGCGTCCCGGCAAGCTCGCCGTCATCGGCAATGGCGTCGTGGTCGATCCGCATGCGCTGATT
GCTGAAATCGGCCGTCTGGAAGTGCAGGGCGTCAAGGTCACGCCGGAAAACCTGCGCATCGCCGACAATGCCACGCTCAT
TCTTTCCCTGCACCGCGAACTGGATGGCATGCGCGAAGACGCCGCCTCCAACAGCGGCACCAAGATTGGCACCACCCGTC
GCGGCATCGGCCCGGCTTACGAAGACAAGGTTGGCCGCCGCGCCATCCGCGTTATGGATCTCGCCGACATGGAGGCGCTC
TCCGCCAAGGTCGATCGCATCCTGACGCATCACAATGCACTTCGCCGTGGTTTCGGCGCGACCGAGGTGTCGCATGAAAC
CATCATGGAAGAGCTGGGCTCGATTGCCGAGCGCATCCTGCCGTTCAGCGAAACCGTCTGGCTGCTGCTCGACAAGAAGC
GCCGCGCCGGTGCGCGCATCCTGTTTGAGGGCGCTCAAGGCTCGCTGCTTGATATCGACCACGGCACATACCCTTATGTG
ACCTCGTCCAACACGGTTGCCGGTCAGGCCGCTGCCGGTTCGGGCATGGGTCCGGGATCGCTCGGTTATATTCTCGGCAT
CACCAAGGCTTACACCACCCGCGTGGGCGAAGGCCCGTTCCCGACCGAGCTGCATGACGAAATCGGCCAGTTCCTCGGTG
AAAAGGGTCATGAATTCGGCACCGTCACCGGCCGCAAGCGCCGCTGCGGCTGGTTCGATGCGGCACTGGTGCGCCAGTCG
GTCGCCACCAACGGTATTACCGGCATCGCGCTGACCAAGCTTGACGTTCTCGACGGTCTCGACGAGCTGAAAATCTGCGT
CGGCTACAAGCTTGACGGTCAGGAAATCGACCATCTTCCGGCAAGCCAGGGTGCGCAGGCTCGTGTTGAGCCTATCTATG
TCACGTTGGAAGGCTGGAAAGAATCTACCGTCGGTGCCCGAAAATGGGCGGATTTGCCTGCTCAGGCAATCAAATATGTC
CGTCAGGTCGAAGAGCTGATCGGGGCGCCTGTCGCGCTTCTGTCGACAAGTCCGGAGCGTGACGACACCATACTTGTGAC
TGATCCGTTTGAGGACTAA

Upstream 100 bases:

>100_bases
ACAGGGTCGCTTTCCGTAAAACAACGCAGTATACATTCGCGGGTTTGGGTTTCCCATCGGGTTATCGGGCGCGCAAGCGC
CATGAGCAGGAAAGCAAGAA

Downstream 100 bases:

>100_bases
TGTCCTCGGCGATCTAGATTCATAAACCATATCGGGCCGGTACGTCGCCGGTCACGACGAGAAAGTGCTTATGGCGGATT
TTGTAGCAGTCATTCGCAAG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTNVVVVGSQWGDEGKGKIVDWLSERADVVVRYQGGHNAGHTLVIDGISYKLSLLPSGVVRPGKLAVIGNGVVVDPHALI
AEIGRLEVQGVKVTPENLRIADNATLILSLHRELDGMREDAASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADMEAL
SAKVDRILTHHNALRRGFGATEVSHETIMEELGSIAERILPFSETVWLLLDKKRRAGARILFEGAQGSLLDIDHGTYPYV
TSSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELHDEIGQFLGEKGHEFGTVTGRKRRCGWFDAALVRQS
VATNGITGIALTKLDVLDGLDELKICVGYKLDGQEIDHLPASQGAQARVEPIYVTLEGWKESTVGARKWADLPAQAIKYV
RQVEELIGAPVALLSTSPERDDTILVTDPFED

Sequences:

>Translated_432_residues
MTNVVVVGSQWGDEGKGKIVDWLSERADVVVRYQGGHNAGHTLVIDGISYKLSLLPSGVVRPGKLAVIGNGVVVDPHALI
AEIGRLEVQGVKVTPENLRIADNATLILSLHRELDGMREDAASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADMEAL
SAKVDRILTHHNALRRGFGATEVSHETIMEELGSIAERILPFSETVWLLLDKKRRAGARILFEGAQGSLLDIDHGTYPYV
TSSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELHDEIGQFLGEKGHEFGTVTGRKRRCGWFDAALVRQS
VATNGITGIALTKLDVLDGLDELKICVGYKLDGQEIDHLPASQGAQARVEPIYVTLEGWKESTVGARKWADLPAQAIKYV
RQVEELIGAPVALLSTSPERDDTILVTDPFED
>Mature_431_residues
TNVVVVGSQWGDEGKGKIVDWLSERADVVVRYQGGHNAGHTLVIDGISYKLSLLPSGVVRPGKLAVIGNGVVVDPHALIA
EIGRLEVQGVKVTPENLRIADNATLILSLHRELDGMREDAASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADMEALS
AKVDRILTHHNALRRGFGATEVSHETIMEELGSIAERILPFSETVWLLLDKKRRAGARILFEGAQGSLLDIDHGTYPYVT
SSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELHDEIGQFLGEKGHEFGTVTGRKRRCGWFDAALVRQSV
ATNGITGIALTKLDVLDGLDELKICVGYKLDGQEIDHLPASQGAQARVEPIYVTLEGWKESTVGARKWADLPAQAIKYVR
QVEELIGAPVALLSTSPERDDTILVTDPFED

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=423, Percent_Identity=44.6808510638298, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=42.5882352941177, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=43.1472081218274, Blast_Score=324, Evalue=1e-88,
Organism=Escherichia coli, GI1790620, Length=432, Percent_Identity=51.8518518518518, Blast_Score=446, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=45.1388888888889, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=45.1388888888889, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=41.2037037037037, Blast_Score=334, Evalue=1e-92,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=43.1603773584906, Blast_Score=343, Evalue=1e-94,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_AGRT5 (Q8UCN6)

Other databases:

- EMBL:   AE007869
- PIR:   AE2877
- PIR:   G97653
- RefSeq:   NP_355399.1
- ProteinModelPortal:   Q8UCN6
- SMR:   Q8UCN6
- STRING:   Q8UCN6
- GeneID:   1134485
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2447
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   Q8UCN6
- ProtClustDB:   PRK01117
- BioCyc:   ATUM176299-1:ATU2447-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46514; Mature: 46382

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 132-132 BINDING 146-146 BINDING 226-226 BINDING 241-241 BINDING 305-305 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVVVVGSQWGDEGKGKIVDWLSERADVVVRYQGGHNAGHTLVIDGISYKLSLLPSGVV
CCCEEEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCC
RPGKLAVIGNGVVVDPHALIAEIGRLEVQGVKVTPENLRIADNATLILSLHRELDGMRED
CCCCEEEEECCEEECCHHHHHHHCCEEEEEEEECCCCEEEECCCEEEEEEHHHHCHHHHH
AASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADMEALSAKVDRILTHHNALRRGFGA
HCCCCCCCCCCCCCCCCCHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TEVSHETIMEELGSIAERILPFSETVWLLLDKKRRAGARILFEGAQGSLLDIDHGTYPYV
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCCCEEECCCCCCCEE
TSSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELHDEIGQFLGEKGHEFG
ECCCCCCCCCCCCCCCCCCCCHHHEEHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEE
TVTGRKRRCGWFDAALVRQSVATNGITGIALTKLDVLDGLDELKICVGYKLDGQEIDHLP
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCEEEEEEEEECCCCCCCCC
ASQGAQARVEPIYVTLEGWKESTVGARKWADLPAQAIKYVRQVEELIGAPVALLSTSPER
CCCCCCCEEEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCC
DDTILVTDPFED
CCEEEEECCCCC
>Mature Secondary Structure 
TNVVVVGSQWGDEGKGKIVDWLSERADVVVRYQGGHNAGHTLVIDGISYKLSLLPSGVV
CCEEEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCC
RPGKLAVIGNGVVVDPHALIAEIGRLEVQGVKVTPENLRIADNATLILSLHRELDGMRED
CCCCEEEEECCEEECCHHHHHHHCCEEEEEEEECCCCEEEECCCEEEEEEHHHHCHHHHH
AASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADMEALSAKVDRILTHHNALRRGFGA
HCCCCCCCCCCCCCCCCCHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TEVSHETIMEELGSIAERILPFSETVWLLLDKKRRAGARILFEGAQGSLLDIDHGTYPYV
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCCCEEECCCCCCCEE
TSSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELHDEIGQFLGEKGHEFG
ECCCCCCCCCCCCCCCCCCCCHHHEEHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEE
TVTGRKRRCGWFDAALVRQSVATNGITGIALTKLDVLDGLDELKICVGYKLDGQEIDHLP
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCEEEEEEEEECCCCCCCCC
ASQGAQARVEPIYVTLEGWKESTVGARKWADLPAQAIKYVRQVEELIGAPVALLSTSPER
CCCCCCCEEEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCC
DDTILVTDPFED
CCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194