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The map label for this gene is rutR [H]

Identifier: 15889655

GI number: 15889655

Start: 2354405

End: 2355046

Strand: Reverse

Name: rutR [H]

Synonym: Atu2384

Alternate gene names: 15889655

Gene position: 2355046-2354405 (Counterclockwise)

Preceding gene: 15889666

Following gene: 159185186

Centisome position: 82.88

GC content: 58.26

Gene sequence:

>642_bases
ATGGCCATACCGCGAGCAGCGAAAACCCAGAAGCGGACCCGCATTCAGGAAGAGAAGCAGGAAACGATTCTCGAGGCTGC
GCTCAGTGTGTTTTCCACCAATGGCTTCCGTGGTTCCACCATCGACCAGATTGCCGAGGCGGCCGGCATGTCGAAACCGA
ACGTGCTTTATTATTTCCGCACCAAGGAAGCGATGCATCGCGCCCTGATCGAACGGGTGCTGGATACGTGGCTCGATCCG
CTGCGCGCCTTTGACGCCGAAGGCAATCCCGAAAGCGAAATCCGCTCCTATATTCGCCGCAAGCTGGAAATGTCGCGGGA
TTTTCCGCGTGAAAGCCGGCTCTTCGCCAATGAAATCCTGCAGGGTGCGCCGCATATAGAGGATGAACTGAAAGGCCCGC
TCAAACAACTGGTGGATGAAAAGGCCGAGGTGATCCGCGCCTGGATCAAGGCGGGGCGGATGGCCAAATGCGATCCTTAC
CATCTGATCTTCTCGATCTGGTCGACCACCCAGCATTATGCGGATTTCGACGTGCAGGTGCGCGCCGTGCTGGGTGACAA
ACACGGTGGCGACGGTCGTTTCGAGGATGCGGCCCGGCATCTGGAGCAGCTTTTCATGGGCGGATTGCGCATCAGCGGCT
GA

Upstream 100 bases:

>100_bases
TCGTGACGAATCGCACTAATTTTGCCTAAACTGTACGCGCAGATTATTTTTTAAGCATACTGTATCGGCAATTGCACTGA
AAAGAAACAGAGAGCAGAGT

Downstream 100 bases:

>100_bases
AAGCCAGGGCTCACGAACAGGTGATCCATTCTTTCTGCATAAACATGAGGAAAATCATCACCTTTATACTTTACTCCAAA
ACTCATCTTTATTATGGTGC

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MAIPRAAKTQKRTRIQEEKQETILEAALSVFSTNGFRGSTIDQIAEAAGMSKPNVLYYFRTKEAMHRALIERVLDTWLDP
LRAFDAEGNPESEIRSYIRRKLEMSRDFPRESRLFANEILQGAPHIEDELKGPLKQLVDEKAEVIRAWIKAGRMAKCDPY
HLIFSIWSTTQHYADFDVQVRAVLGDKHGGDGRFEDAARHLEQLFMGGLRISG

Sequences:

>Translated_213_residues
MAIPRAAKTQKRTRIQEEKQETILEAALSVFSTNGFRGSTIDQIAEAAGMSKPNVLYYFRTKEAMHRALIERVLDTWLDP
LRAFDAEGNPESEIRSYIRRKLEMSRDFPRESRLFANEILQGAPHIEDELKGPLKQLVDEKAEVIRAWIKAGRMAKCDPY
HLIFSIWSTTQHYADFDVQVRAVLGDKHGGDGRFEDAARHLEQLFMGGLRISG
>Mature_212_residues
AIPRAAKTQKRTRIQEEKQETILEAALSVFSTNGFRGSTIDQIAEAAGMSKPNVLYYFRTKEAMHRALIERVLDTWLDPL
RAFDAEGNPESEIRSYIRRKLEMSRDFPRESRLFANEILQGAPHIEDELKGPLKQLVDEKAEVIRAWIKAGRMAKCDPYH
LIFSIWSTTQHYADFDVQVRAVLGDKHGGDGRFEDAARHLEQLFMGGLRISG

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=202, Percent_Identity=49.009900990099, Blast_Score=208, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 24333; Mature: 24202

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPRAAKTQKRTRIQEEKQETILEAALSVFSTNGFRGSTIDQIAEAAGMSKPNVLYYFR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEC
TKEAMHRALIERVLDTWLDPLRAFDAEGNPESEIRSYIRRKLEMSRDFPRESRLFANEIL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QGAPHIEDELKGPLKQLVDEKAEVIRAWIKAGRMAKCDPYHLIFSIWSTTQHYADFDVQV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
RAVLGDKHGGDGRFEDAARHLEQLFMGGLRISG
HEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AIPRAAKTQKRTRIQEEKQETILEAALSVFSTNGFRGSTIDQIAEAAGMSKPNVLYYFR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEC
TKEAMHRALIERVLDTWLDPLRAFDAEGNPESEIRSYIRRKLEMSRDFPRESRLFANEIL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QGAPHIEDELKGPLKQLVDEKAEVIRAWIKAGRMAKCDPYHLIFSIWSTTQHYADFDVQV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
RAVLGDKHGGDGRFEDAARHLEQLFMGGLRISG
HEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]