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The map label for this gene is hisJ [H]

Identifier: 15889639

GI number: 15889639

Start: 2339896

End: 2340666

Strand: Reverse

Name: hisJ [H]

Synonym: Atu2365

Alternate gene names: 15889639

Gene position: 2340666-2339896 (Counterclockwise)

Preceding gene: 15889640

Following gene: 15889638

Centisome position: 82.37

GC content: 56.03

Gene sequence:

>771_bases
ATGAAATTCTTCGCCACGCTGCTTGCCGGAACGGCATTTGCGGTTTCCGCCTTCACCGCCAGCGCCGATGTCCGCTTCGG
CATCATGAACGAATCCTACCCGCCCTTCTTTGCCAAGGATGCCAGCGGCAAGTGGCAGGGCTGGGAAATCGATCTCATGG
ACGCCGTCTGCGCGGAAATGAAAGAAAAGTGCTCGATCGTCGAGCTTTCCTGGGATGGCCTCATCCCGGCGCTGCAAACG
AAGAAGTTCGATGTCATCTGGTCGTCCATGTCCAACACGCAGGAGCGCGAGAAGGTCATCGATTTCACCAACAAATATTA
CAACACGCCAAGCAAGCTGATCGGCGCCAAGGGTGAAAAGCCGGGTGCGGCACCGGAAGACGTCAAGGGCAAGACCATCG
GCATTCAGGTCGCGACCATCCAGTCCGAATATTACAAGAAATATTTCGCCGCTGTTGCTGACGAAAAGACCTACCAGACG
CTGGACGAGGCGTTTCAGGATCTGGCCGCCGGCCGTATCGATTATGTCTTCGGCGACTCGCTCGTTCTCGATGCCTTCGT
CAAGAGCGATGCCGGCAAGGATTGCTGCGCCGACATGGGCAATGTGGCGGACGACAAGGAAATCATGGGTCTTGGCGTTT
CCGGCGGCCTGCGCAAGGACGACACCGAACTCAAGAACAAGCTGAATGCTGCTATCGCTGCCGTGCGCGCCAGCGGGAAG
TATGACGAGATCACCAAGAAATACTTCAGCTTCGATATCTACGGTAACTAA

Upstream 100 bases:

>100_bases
TGTTCGGCAATCCGTTGAGCGCCCGTTGCAGGGAATTTACCGGCGCGCTGGCGTAATGGCCGTTTCGCTTCGCCGTTTGT
TTTAACAGGAGAGACTTAAA

Downstream 100 bases:

>100_bases
TTTCTCCTGACCGGTCACATCAATGGCAAGCCTTGAAACCACGCTGCAACTGCTGTCACCCTATCCTCCGGGATGGGGCG
GCACCCTTTTGAAGGGTGCG

Product: ABC transporter, substrate binding protein (amino acid)

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKFFATLLAGTAFAVSAFTASADVRFGIMNESYPPFFAKDASGKWQGWEIDLMDAVCAEMKEKCSIVELSWDGLIPALQT
KKFDVIWSSMSNTQEREKVIDFTNKYYNTPSKLIGAKGEKPGAAPEDVKGKTIGIQVATIQSEYYKKYFAAVADEKTYQT
LDEAFQDLAAGRIDYVFGDSLVLDAFVKSDAGKDCCADMGNVADDKEIMGLGVSGGLRKDDTELKNKLNAAIAAVRASGK
YDEITKKYFSFDIYGN

Sequences:

>Translated_256_residues
MKFFATLLAGTAFAVSAFTASADVRFGIMNESYPPFFAKDASGKWQGWEIDLMDAVCAEMKEKCSIVELSWDGLIPALQT
KKFDVIWSSMSNTQEREKVIDFTNKYYNTPSKLIGAKGEKPGAAPEDVKGKTIGIQVATIQSEYYKKYFAAVADEKTYQT
LDEAFQDLAAGRIDYVFGDSLVLDAFVKSDAGKDCCADMGNVADDKEIMGLGVSGGLRKDDTELKNKLNAAIAAVRASGK
YDEITKKYFSFDIYGN
>Mature_256_residues
MKFFATLLAGTAFAVSAFTASADVRFGIMNESYPPFFAKDASGKWQGWEIDLMDAVCAEMKEKCSIVELSWDGLIPALQT
KKFDVIWSSMSNTQEREKVIDFTNKYYNTPSKLIGAKGEKPGAAPEDVKGKTIGIQVATIQSEYYKKYFAAVADEKTYQT
LDEAFQDLAAGRIDYVFGDSLVLDAFVKSDAGKDCCADMGNVADDKEIMGLGVSGGLRKDDTELKNKLNAAIAAVRASGK
YDEITKKYFSFDIYGN

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=39.1489361702128, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI1788649, Length=254, Percent_Identity=38.9763779527559, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1787085, Length=246, Percent_Identity=33.739837398374, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=33.0645161290323, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1787031, Length=239, Percent_Identity=29.2887029288703, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788228, Length=224, Percent_Identity=29.4642857142857, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28120; Mature: 28120

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFATLLAGTAFAVSAFTASADVRFGIMNESYPPFFAKDASGKWQGWEIDLMDAVCAEM
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCCCCEEEHHHHHHHHHH
KEKCSIVELSWDGLIPALQTKKFDVIWSSMSNTQEREKVIDFTNKYYNTPSKLIGAKGEK
HHCCCEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHCCCCCC
PGAAPEDVKGKTIGIQVATIQSEYYKKYFAAVADEKTYQTLDEAFQDLAAGRIDYVFGDS
CCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCH
LVLDAFVKSDAGKDCCADMGNVADDKEIMGLGVSGGLRKDDTELKNKLNAAIAAVRASGK
HHHHHHHHCCCCCHHHHHCCCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCC
YDEITKKYFSFDIYGN
HHHHHHHHHEEEECCC
>Mature Secondary Structure
MKFFATLLAGTAFAVSAFTASADVRFGIMNESYPPFFAKDASGKWQGWEIDLMDAVCAEM
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCCCCEEEHHHHHHHHHH
KEKCSIVELSWDGLIPALQTKKFDVIWSSMSNTQEREKVIDFTNKYYNTPSKLIGAKGEK
HHCCCEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHCCCCCC
PGAAPEDVKGKTIGIQVATIQSEYYKKYFAAVADEKTYQTLDEAFQDLAAGRIDYVFGDS
CCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCH
LVLDAFVKSDAGKDCCADMGNVADDKEIMGLGVSGGLRKDDTELKNKLNAAIAAVRASGK
HHHHHHHHCCCCCHHHHHCCCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCC
YDEITKKYFSFDIYGN
HHHHHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]