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The map label for this gene is braC [C]

Identifier: 15889551

GI number: 15889551

Start: 2247389

End: 2248495

Strand: Reverse

Name: braC [C]

Synonym: Atu2276

Alternate gene names: 15889551

Gene position: 2248495-2247389 (Counterclockwise)

Preceding gene: 159185137

Following gene: 15889550

Centisome position: 79.13

GC content: 60.52

Gene sequence:

>1107_bases
ATGAAACTGAAGACATTGACCAGCGTGACGCTCGCAGCGTCCTTCGCATTCGCACCCCTTGCCCATGCCGAAATCGTCAT
CGGCCTGATTGCGCCGCTCACCGGCCCCGTTGCTGCCTATGGCGACCAGGTCAAGAATGGCGCCCAGACGGCAGTCAGTG
AAATCAATAAAAAGGGCGGCATCCTCGGCGAACAGGTCGTGCTGAAGCTCGCTGACGATGGCGGCGAGCCCAAGCAGGGC
GTTTCCGCGGCCAACCAGCTGGTTGCCGAAGGCATTCATTTCGTCGTCGGTCCGGTGACATCGGGCGTTGCCATTCCGGC
ATCGGATGTCTTCGCCGAAAACGGCGTCCTGATGGTCACGCCGACGGCGACCGCGCCGGGACTGACCAATCGCGGTCTTT
CCAACGTTTTCCGCACCTGCGGTCGTGATGACCAGCAGGCCGAGGTTGCGGCGAAATACGTTCTCGCCAACCTCAAGGGC
AAGAAGATCGCCATCATTCACGACAAGGGCGCTTACGGTAAGGGTCTTGCCGATTCCTTCAAGGCGACGCTGAATGCCGG
CGGCGTTACCGAAGTTCTCTACGATGCGCTGACACCTGGCGAAAAGGATCTCGGCGCGCTGACGGCCCGCCTGAAGTCCG
ACAATGCGGACATCGTCTATTTCGGCGGTTACCATCCGGAAGCTGGTCTTCTGGTTCGCCAGCTGAAGGATATCGGCTCC
AAGGCTGCCGTCATCGGCGGTGATGGCCTGTCGAACAGCGAATTCTGGAACATCGGCGCGAAGGCCGGCGAAGGCACGAT
CTTCACCAACGCTTCCGACGCCCTGAAGAACGATGATTCCAAGGCCGCTGCCGAAGCCCTGAAGGCCGCCAACATTCCGG
CTGAAGCCTTCACGCTCAACGCCTATGCCGCCGTCGAAGTCTTGAAGGCCGGTATCGAAAAGGCCGGCAGCGCCAAGGAT
TCCGAGGCGGTCGCCAAGGTTCTGAAAAGCGGCGAGGCTTTCCCGACCGCAATCGGCAAGGTCACCTATGGTGACAATGG
CGACCTGACCTCGCAGGCCTTCTCGCTCTTCAAGTGGCAGGACGGCAAGATCGTCGCAGCCGAATAA

Upstream 100 bases:

>100_bases
ACATGGAACGAGTTTTCGCCGGGAGGCGTCCGATAGTTTCATTCGGTCGGCGCCCATGGCGCTCACCGGGTCCGCGACCC
CTTTTTAAGGAGACACCAGG

Downstream 100 bases:

>100_bases
GCTGATCGAACCGGCCGCACCTGTTGCGGCCGGTTTCATTTTACGGTCTGAAAAGAGGTAGGGCAGCGTGGTCGATGCCG
AAACGCAAACCTTTGGGGAA

Product: ABC transporter, substrate binding protein (branched chain amino acid)

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKLKTLTSVTLAASFAFAPLAHAEIVIGLIAPLTGPVAAYGDQVKNGAQTAVSEINKKGGILGEQVVLKLADDGGEPKQG
VSAANQLVAEGIHFVVGPVTSGVAIPASDVFAENGVLMVTPTATAPGLTNRGLSNVFRTCGRDDQQAEVAAKYVLANLKG
KKIAIIHDKGAYGKGLADSFKATLNAGGVTEVLYDALTPGEKDLGALTARLKSDNADIVYFGGYHPEAGLLVRQLKDIGS
KAAVIGGDGLSNSEFWNIGAKAGEGTIFTNASDALKNDDSKAAAEALKAANIPAEAFTLNAYAAVEVLKAGIEKAGSAKD
SEAVAKVLKSGEAFPTAIGKVTYGDNGDLTSQAFSLFKWQDGKIVAAE

Sequences:

>Translated_368_residues
MKLKTLTSVTLAASFAFAPLAHAEIVIGLIAPLTGPVAAYGDQVKNGAQTAVSEINKKGGILGEQVVLKLADDGGEPKQG
VSAANQLVAEGIHFVVGPVTSGVAIPASDVFAENGVLMVTPTATAPGLTNRGLSNVFRTCGRDDQQAEVAAKYVLANLKG
KKIAIIHDKGAYGKGLADSFKATLNAGGVTEVLYDALTPGEKDLGALTARLKSDNADIVYFGGYHPEAGLLVRQLKDIGS
KAAVIGGDGLSNSEFWNIGAKAGEGTIFTNASDALKNDDSKAAAEALKAANIPAEAFTLNAYAAVEVLKAGIEKAGSAKD
SEAVAKVLKSGEAFPTAIGKVTYGDNGDLTSQAFSLFKWQDGKIVAAE
>Mature_368_residues
MKLKTLTSVTLAASFAFAPLAHAEIVIGLIAPLTGPVAAYGDQVKNGAQTAVSEINKKGGILGEQVVLKLADDGGEPKQG
VSAANQLVAEGIHFVVGPVTSGVAIPASDVFAENGVLMVTPTATAPGLTNRGLSNVFRTCGRDDQQAEVAAKYVLANLKG
KKIAIIHDKGAYGKGLADSFKATLNAGGVTEVLYDALTPGEKDLGALTARLKSDNADIVYFGGYHPEAGLLVRQLKDIGS
KAAVIGGDGLSNSEFWNIGAKAGEGTIFTNASDALKNDDSKAAAEALKAANIPAEAFTLNAYAAVEVLKAGIEKAGSAKD
SEAVAKVLKSGEAFPTAIGKVTYGDNGDLTSQAFSLFKWQDGKIVAAE

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=349, Percent_Identity=40.6876790830946, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI1789867, Length=367, Percent_Identity=39.2370572207085, Blast_Score=260, Evalue=9e-71,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 37660; Mature: 37660

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTLTSVTLAASFAFAPLAHAEIVIGLIAPLTGPVAAYGDQVKNGAQTAVSEINKKGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCC
ILGEQVVLKLADDGGEPKQGVSAANQLVAEGIHFVVGPVTSGVAIPASDVFAENGVLMVT
CCHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHCCCEEEEE
PTATAPGLTNRGLSNVFRTCGRDDQQAEVAAKYVLANLKGKKIAIIHDKGAYGKGLADSF
CCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
KATLNAGGVTEVLYDALTPGEKDLGALTARLKSDNADIVYFGGYHPEAGLLVRQLKDIGS
HHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCC
KAAVIGGDGLSNSEFWNIGAKAGEGTIFTNASDALKNDDSKAAAEALKAANIPAEAFTLN
CEEEEECCCCCCCCEEECCCCCCCCEEEECCHHHHCCCCHHHHHHHHHHCCCCHHHEEEH
AYAAVEVLKAGIEKAGSAKDSEAVAKVLKSGEAFPTAIGKVTYGDNGDLTSQAFSLFKWQ
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHCEEEECCCCCHHHHHHHHHEEC
DGKIVAAE
CCEEEECC
>Mature Secondary Structure
MKLKTLTSVTLAASFAFAPLAHAEIVIGLIAPLTGPVAAYGDQVKNGAQTAVSEINKKGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCC
ILGEQVVLKLADDGGEPKQGVSAANQLVAEGIHFVVGPVTSGVAIPASDVFAENGVLMVT
CCHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHCCCEEEEE
PTATAPGLTNRGLSNVFRTCGRDDQQAEVAAKYVLANLKGKKIAIIHDKGAYGKGLADSF
CCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
KATLNAGGVTEVLYDALTPGEKDLGALTARLKSDNADIVYFGGYHPEAGLLVRQLKDIGS
HHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCC
KAAVIGGDGLSNSEFWNIGAKAGEGTIFTNASDALKNDDSKAAAEALKAANIPAEAFTLN
CEEEEECCCCCCCCEEECCCCCCCCEEEECCHHHHCCCCHHHHHHHHHHCCCCHHHEEEH
AYAAVEVLKAGIEKAGSAKDSEAVAKVLKSGEAFPTAIGKVTYGDNGDLTSQAFSLFKWQ
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHCEEEECCCCCHHHHHHHHHEEC
DGKIVAAE
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]