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The map label for this gene is nemA [H]

Identifier: 15889468

GI number: 15889468

Start: 2164070

End: 2165182

Strand: Direct

Name: nemA [H]

Synonym: Atu2188

Alternate gene names: 15889468

Gene position: 2164070-2165182 (Clockwise)

Preceding gene: 159185093

Following gene: 15889471

Centisome position: 76.16

GC content: 61.28

Gene sequence:

>1113_bases
ATGACCAGTCTTTTCGAACCGGCACAGGCCGGCGATATCGCACTCGCCAACCGTATCGTCATGGCTCCCCTCACCCGCAA
CCGTTCGCCGGGGGCAATTCCCAACAATCTCAACGCCACCTATTACGAGCAGCGCGCCACGGCTGGGCTGATCGTCACCG
AAGGTACGCCGATTTCCCAGCAGGGTCAGGGTTACGCTGACGTTCCGGGCCTCTACAAGCAGGAAGCGATCGAGGGCTGG
AAAAAGATCACCGACGGCGTGCACTCGGCCGGCGGCAAGATCGTTGCGCAGATCTGGCATGTGGGCCGCATTTCCCATAC
TTCGCTGCAGCCGCATGGCGGCCAGCCGGTCGCACCATCAGCCATTCCTGCCAAGTCGAAGACCTATATCATCAATGATG
ACGGCACCGGCGCCTTTGCCGAGACCTCCGAGCCCCGCGCGCTCACCATCGACGATATCGGCCTCATCCTCGAGGATTAT
CGTAGCGGTGCACGCGCAGCCCTGGAGGCCGGTTTCGACGGCGTCGAAATCCATGCCGCCAACGGTTACCTGATCGAGCA
GTTCCTGAAATCCAGCACCAACCAGCGCACGGATGATTACGGCGGCTCGATCGAAAACCGCGCCCGCTTCCTGCTGGAAG
TTGTCGACGCGGTTGCGGAAGAGATCGGCGCGGGCCGCACCGGCATCCGCCTCTCCCCCGTCACCCCGGCCAACGATATT
TTCGAGGCCGATCCGCAGCCGCTTTATAATTACGTGGTGGAAGAACTCGGCAAGCGCAATCTCGCCTTCATCCATGTGGT
CGAAGGCGCAACGGGCGGACCGCGTGATTTCAAACAGGGCGACAAGCCGTTCGATTACGCGACCTTCAAGGGCGCCTATC
GCAATGCAGGCGGCAAGGGCCTGTGGATTGCCAATAATGGTTACGACCGCGAGAGCGCCATCGAAGCCGTGGAAAGCGGC
AAAGTCGATGCCGTCGCCTTCGGCAAGGCCTTCATCGCCAATCCGGATCTGGTGCGTCGCCTCAAAGACAACGCGCCCTT
GAACGAACCGAACCAGCCGACCTTCTATGGCGGCGGAGCCGAAGGTTATACCGACTATCCGGCACTCGGCTGA

Upstream 100 bases:

>100_bases
GGGTGCTGGACTGGAAACCACAGCGGTTTTCGTTCCGGCACCCGAAAAACAAAGCGCGCCTGCCGCGCAATTACGCCAAC
CGACCAGAAAGTGAAGTCGC

Downstream 100 bases:

>100_bases
TAAACTGAAACGCCGCACCGCCCTTTGGCTGTGCGGCATTTTTCTTTCAGCGCGGGCCTCTCGCCTGCCGCGCGGCAAAA
TCTGAAAACGCACCCATGAT

Product: oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTSLFEPAQAGDIALANRIVMAPLTRNRSPGAIPNNLNATYYEQRATAGLIVTEGTPISQQGQGYADVPGLYKQEAIEGW
KKITDGVHSAGGKIVAQIWHVGRISHTSLQPHGGQPVAPSAIPAKSKTYIINDDGTGAFAETSEPRALTIDDIGLILEDY
RSGARAALEAGFDGVEIHAANGYLIEQFLKSSTNQRTDDYGGSIENRARFLLEVVDAVAEEIGAGRTGIRLSPVTPANDI
FEADPQPLYNYVVEELGKRNLAFIHVVEGATGGPRDFKQGDKPFDYATFKGAYRNAGGKGLWIANNGYDRESAIEAVESG
KVDAVAFGKAFIANPDLVRRLKDNAPLNEPNQPTFYGGGAEGYTDYPALG

Sequences:

>Translated_370_residues
MTSLFEPAQAGDIALANRIVMAPLTRNRSPGAIPNNLNATYYEQRATAGLIVTEGTPISQQGQGYADVPGLYKQEAIEGW
KKITDGVHSAGGKIVAQIWHVGRISHTSLQPHGGQPVAPSAIPAKSKTYIINDDGTGAFAETSEPRALTIDDIGLILEDY
RSGARAALEAGFDGVEIHAANGYLIEQFLKSSTNQRTDDYGGSIENRARFLLEVVDAVAEEIGAGRTGIRLSPVTPANDI
FEADPQPLYNYVVEELGKRNLAFIHVVEGATGGPRDFKQGDKPFDYATFKGAYRNAGGKGLWIANNGYDRESAIEAVESG
KVDAVAFGKAFIANPDLVRRLKDNAPLNEPNQPTFYGGGAEGYTDYPALG
>Mature_369_residues
TSLFEPAQAGDIALANRIVMAPLTRNRSPGAIPNNLNATYYEQRATAGLIVTEGTPISQQGQGYADVPGLYKQEAIEGWK
KITDGVHSAGGKIVAQIWHVGRISHTSLQPHGGQPVAPSAIPAKSKTYIINDDGTGAFAETSEPRALTIDDIGLILEDYR
SGARAALEAGFDGVEIHAANGYLIEQFLKSSTNQRTDDYGGSIENRARFLLEVVDAVAEEIGAGRTGIRLSPVTPANDIF
EADPQPLYNYVVEELGKRNLAFIHVVEGATGGPRDFKQGDKPFDYATFKGAYRNAGGKGLWIANNGYDRESAIEAVESGK
VDAVAFGKAFIANPDLVRRLKDNAPLNEPNQPTFYGGGAEGYTDYPALG

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=369, Percent_Identity=49.8644986449864, Blast_Score=334, Evalue=5e-93,
Organism=Escherichia coli, GI1789463, Length=223, Percent_Identity=36.322869955157, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17559802, Length=277, Percent_Identity=28.8808664259928, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17559804, Length=277, Percent_Identity=28.8808664259928, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=359, Percent_Identity=27.5766016713092, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=200, Percent_Identity=30.5, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=272, Percent_Identity=26.1029411764706, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=137, Percent_Identity=36.4963503649635, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=180, Percent_Identity=30, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=385, Percent_Identity=40.7792207792208, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6321973, Length=385, Percent_Identity=38.4415584415584, Blast_Score=222, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39584; Mature: 39453

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLFEPAQAGDIALANRIVMAPLTRNRSPGAIPNNLNATYYEQRATAGLIVTEGTPISQ
CCCCCCCCCCCCCHHHCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCCCC
QGQGYADVPGLYKQEAIEGWKKITDGVHSAGGKIVAQIWHVGRISHTSLQPHGGQPVAPS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
AIPAKSKTYIINDDGTGAFAETSEPRALTIDDIGLILEDYRSGARAALEAGFDGVEIHAA
CCCCCCCEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCHHHHHHHCCCCCEEEEEC
NGYLIEQFLKSSTNQRTDDYGGSIENRARFLLEVVDAVAEEIGAGRTGIRLSPVTPANDI
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHH
FEADPQPLYNYVVEELGKRNLAFIHVVEGATGGPRDFKQGDKPFDYATFKGAYRNAGGKG
HCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHCCCCCCCHHHHCHHHHCCCCCE
LWIANNGYDRESAIEAVESGKVDAVAFGKAFIANPDLVRRLKDNAPLNEPNQPTFYGGGA
EEEECCCCCHHHHHHHHHCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCCCCCEEECCCC
EGYTDYPALG
CCCCCCCCCC
>Mature Secondary Structure 
TSLFEPAQAGDIALANRIVMAPLTRNRSPGAIPNNLNATYYEQRATAGLIVTEGTPISQ
CCCCCCCCCCCCHHHCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCCCC
QGQGYADVPGLYKQEAIEGWKKITDGVHSAGGKIVAQIWHVGRISHTSLQPHGGQPVAPS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
AIPAKSKTYIINDDGTGAFAETSEPRALTIDDIGLILEDYRSGARAALEAGFDGVEIHAA
CCCCCCCEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCHHHHHHHCCCCCEEEEEC
NGYLIEQFLKSSTNQRTDDYGGSIENRARFLLEVVDAVAEEIGAGRTGIRLSPVTPANDI
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHH
FEADPQPLYNYVVEELGKRNLAFIHVVEGATGGPRDFKQGDKPFDYATFKGAYRNAGGKG
HCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHCCCCCCCHHHHCHHHHCCCCCE
LWIANNGYDRESAIEAVESGKVDAVAFGKAFIANPDLVRRLKDNAPLNEPNQPTFYGGGA
EEEECCCCCHHHHHHHHHCCCEEEEEECCHHHCCHHHHHHHHHCCCCCCCCCCEEECCCC
EGYTDYPALG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]