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The map label for this gene is yoeB [H]

Identifier: 15889305

GI number: 15889305

Start: 1979333

End: 1979602

Strand: Reverse

Name: yoeB [H]

Synonym: Atu2017

Alternate gene names: 15889305

Gene position: 1979602-1979333 (Counterclockwise)

Preceding gene: 159185012

Following gene: 15889303

Centisome position: 69.67

GC content: 53.33

Gene sequence:

>270_bases
ATGAAGCTCGTCTGGACGCTGAGTTCATGGGACGACTATGAGTTCTGGCAAAGAACCGATGCTCGCATGGTCGAGAAAAT
AAACGATCTTATCCGAAATGCCAAACGCACGCCCTTTGCAGGGCTTGGGAAACCGGAGCCTCTGAAGGGCGATATGGCAG
GATATTGGTCTCGGCGGATTACCGCCGAGCATCGGTTTGTCTACCGTGTATCCGGGTCCGGAAGCGAGCAACGGCTGGAA
GTCATTCAGTGCCGCTTCCATTACCAATAA

Upstream 100 bases:

>100_bases
CGCTGCATCTCCTGTCCAACCCGGCAAATGCCTCAAGGCTTCGCGCGTCCATGGGCGAACTTGAGCGCGGTGACACCATC
GAGCGGGATCCGACCGAAGA

Downstream 100 bases:

>100_bases
CCGCTCACTTTGGCGGGATGACACCCTTGGTCAGCAGAAAGCATCCATAAAACCGCGTCTCGCCTGTGGTGATGACGCAA
TAGGCCTGCTTGGCCTTTTC

Product: hypothetical protein

Products: NA

Alternate protein names: Putative endoribonuclease YoeB; Putative mRNA interferase Yoeb [H]

Number of amino acids: Translated: 89; Mature: 89

Protein sequence:

>89_residues
MKLVWTLSSWDDYEFWQRTDARMVEKINDLIRNAKRTPFAGLGKPEPLKGDMAGYWSRRITAEHRFVYRVSGSGSEQRLE
VIQCRFHYQ

Sequences:

>Translated_89_residues
MKLVWTLSSWDDYEFWQRTDARMVEKINDLIRNAKRTPFAGLGKPEPLKGDMAGYWSRRITAEHRFVYRVSGSGSEQRLE
VIQCRFHYQ
>Mature_89_residues
MKLVWTLSSWDDYEFWQRTDARMVEKINDLIRNAKRTPFAGLGKPEPLKGDMAGYWSRRITAEHRFVYRVSGSGSEQRLE
VIQCRFHYQ

Specific function: Part of a toxin-antitoxin (TA) module. It has been proposed to be an mRNA interferase but also an inhibitor of translation initiation. Has an in vitro RNase activity and preferentially cleaves at the 3'-end of purine ribonucleotides. YefM binds to the pro

COG id: COG4115

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YoeB family [H]

Homologues:

Organism=Escherichia coli, GI87082025, Length=88, Percent_Identity=56.8181818181818, Blast_Score=114, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009614 [H]

Pfam domain/function: PF06769 Plasmid_Txe [H]

EC number: NA

Molecular weight: Translated: 10612; Mature: 10612

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVWTLSSWDDYEFWQRTDARMVEKINDLIRNAKRTPFAGLGKPEPLKGDMAGYWSRRI
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCC
TAEHRFVYRVSGSGSEQRLEVIQCRFHYQ
CCCCEEEEEECCCCCHHHHHHHHEEECCC
>Mature Secondary Structure
MKLVWTLSSWDDYEFWQRTDARMVEKINDLIRNAKRTPFAGLGKPEPLKGDMAGYWSRRI
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCC
TAEHRFVYRVSGSGSEQRLEVIQCRFHYQ
CCCCEEEEEECCCCCHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]