Click here to switch to the map view.

The map label for this gene is folE

Identifier: 15889053

GI number: 15889053

Start: 1736303

End: 1736929

Strand: Direct

Name: folE

Synonym: Atu1749

Alternate gene names: 15889053

Gene position: 1736303-1736929 (Clockwise)

Preceding gene: 15889048

Following gene: 159184898

Centisome position: 61.1

GC content: 60.77

Gene sequence:

>627_bases
ATGGATGCGATCGTGAAGAATTTCCCCGGCGCTGGCGCAAAACCAGACGTGGCCGAAGCTCGCCCAAGCCAGGCCGAAGC
GGAAGAAGCCGTGCGCGTTCTCCTGCGCTGGGCAGGAGAAAATCCCGCCCGCGAAGGACTTCTGGATACGCCGAAGCGCG
TCGCCAAGGCCTATCGCGAGCTTTTTGCCGGTTATGAGCTGAATGTTCAGGACGTGCTTGGCACGACCTTCGAGGAAGTC
GGCGGTTATGACGATGTGGTGCTGGTGCGCGACATCCCCTTCTTCTCCCATTGCGAGCACCATATGGTGCCGATCGTCGG
CAAGGCGCATGTCGCCTATCTTCCGGCCGGCCGTGTGCTTGGCCTGTCGAAGATCGCCCGCGTCGTCGAAATCTTCGGCC
GCCGCCTGCAGACGCAGGAAAACATGACGGCGCAGATCGCAAGATCGATCGAAGAAACGCTGAAACCGCGTGGCGTAGCC
GTCATGATCGACGCGGAACATATGTGCATGTCCATGCGCGGCGTAAACAAGCAAGGGTCCACGACGCTGACCACCAGCTT
CACCGGCACGTTCAAGAACGATCCGGCCGAGCAGGTGCGCTTCATGACCATGGTGCGGAACCGCTGA

Upstream 100 bases:

>100_bases
CAAAATGCTGTAAAACAACCAGATAGCAGCAGCTGCGAACATGAGTTCGCGATGAAAAACGAATTCAGGTGCGGCCTTTA
CCCGCATCGGGAGAGACGGA

Downstream 100 bases:

>100_bases
ACATCGGACCGGAAACTGTAGCGCGGGTTTCCTGAAACCCGCTGCTCAGACAAAATTTTGGAGTGGCGGACTGATCCCAT
CGTCTCCGCCGCTTTGAAAC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MDAIVKNFPGAGAKPDVAEARPSQAEAEEAVRVLLRWAGENPAREGLLDTPKRVAKAYRELFAGYELNVQDVLGTTFEEV
GGYDDVVLVRDIPFFSHCEHHMVPIVGKAHVAYLPAGRVLGLSKIARVVEIFGRRLQTQENMTAQIARSIEETLKPRGVA
VMIDAEHMCMSMRGVNKQGSTTLTTSFTGTFKNDPAEQVRFMTMVRNR

Sequences:

>Translated_208_residues
MDAIVKNFPGAGAKPDVAEARPSQAEAEEAVRVLLRWAGENPAREGLLDTPKRVAKAYRELFAGYELNVQDVLGTTFEEV
GGYDDVVLVRDIPFFSHCEHHMVPIVGKAHVAYLPAGRVLGLSKIARVVEIFGRRLQTQENMTAQIARSIEETLKPRGVA
VMIDAEHMCMSMRGVNKQGSTTLTTSFTGTFKNDPAEQVRFMTMVRNR
>Mature_208_residues
MDAIVKNFPGAGAKPDVAEARPSQAEAEEAVRVLLRWAGENPAREGLLDTPKRVAKAYRELFAGYELNVQDVLGTTFEEV
GGYDDVVLVRDIPFFSHCEHHMVPIVGKAHVAYLPAGRVLGLSKIARVVEIFGRRLQTQENMTAQIARSIEETLKPRGVA
VMIDAEHMCMSMRGVNKQGSTTLTTSFTGTFKNDPAEQVRFMTMVRNR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=179, Percent_Identity=50.2793296089386, Blast_Score=187, Evalue=6e-48,
Organism=Homo sapiens, GI4503949, Length=179, Percent_Identity=50.2793296089386, Blast_Score=187, Evalue=6e-48,
Organism=Homo sapiens, GI66932970, Length=148, Percent_Identity=48.6486486486487, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI66932972, Length=137, Percent_Identity=51.8248175182482, Blast_Score=142, Evalue=2e-34,
Organism=Escherichia coli, GI1788476, Length=176, Percent_Identity=40.9090909090909, Blast_Score=117, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17560486, Length=180, Percent_Identity=45, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6321706, Length=181, Percent_Identity=45.8563535911602, Blast_Score=172, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24656782, Length=180, Percent_Identity=47.7777777777778, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24656796, Length=180, Percent_Identity=47.7777777777778, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24656791, Length=180, Percent_Identity=47.7777777777778, Blast_Score=174, Evalue=3e-44,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_AGRT5 (Q8UEK8)

Other databases:

- EMBL:   AE007869
- PIR:   AF2791
- PIR:   F97570
- RefSeq:   NP_354734.1
- ProteinModelPortal:   Q8UEK8
- SMR:   Q8UEK8
- STRING:   Q8UEK8
- GeneID:   1133787
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1749
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   LTTTFTG
- PhylomeDB:   Q8UEK8
- ProtClustDB:   PRK09347
- BioCyc:   ATUM176299-1:ATU1749-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 23032; Mature: 23032

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAIVKNFPGAGAKPDVAEARPSQAEAEEAVRVLLRWAGENPAREGLLDTPKRVAKAYRE
CCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHH
LFAGYELNVQDVLGTTFEEVGGYDDVVLVRDIPFFSHCEHHMVPIVGKAHVAYLPAGRVL
HHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCCCCEEECCCEEEEECCCHHH
GLSKIARVVEIFGRRLQTQENMTAQIARSIEETLKPRGVAVMIDAEHMCMSMRGVNKQGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCC
TTLTTSFTGTFKNDPAEQVRFMTMVRNR
EEEEEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDAIVKNFPGAGAKPDVAEARPSQAEAEEAVRVLLRWAGENPAREGLLDTPKRVAKAYRE
CCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHH
LFAGYELNVQDVLGTTFEEVGGYDDVVLVRDIPFFSHCEHHMVPIVGKAHVAYLPAGRVL
HHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCCCCEEECCCEEEEECCCHHH
GLSKIARVVEIFGRRLQTQENMTAQIARSIEETLKPRGVAVMIDAEHMCMSMRGVNKQGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCC
TTLTTSFTGTFKNDPAEQVRFMTMVRNR
EEEEEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194