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The map label for this gene is def [C]

Identifier: 15888871

GI number: 15888871

Start: 1541091

End: 1541585

Strand: Direct

Name: def [C]

Synonym: Atu1550

Alternate gene names: 15888871

Gene position: 1541091-1541585 (Clockwise)

Preceding gene: 15888870

Following gene: 15888873

Centisome position: 54.23

GC content: 58.79

Gene sequence:

>495_bases
TTGGCAATCAGGCCGATCCTGCCCTACCCCCATGCCGGTCTTTCCGGGATCTGCGCGCCAGTTACGGTTTTTGACGATCA
TCTGCGGGAACTGGTAACAGATCTGATCGACACGATGCGCGCTGCCCCCGGCGTCGGCATCACTGCGGCCCATATCGGCG
TGCTGCAACGTGTCTTCGTGCTTGAACTGACGCCGGGAACCATCCTGACCTACATCAATCCTGAAATCACCAGCCACTCT
CCGCAGACCATGCGGCATGTGGAAGGCAGCGTCTCGATGCCGGGCTTCACCGACGAGGTGGAACGGCCTTCTACGGTCGA
GGTGAGGTTTCAGGATATAACTGGCGCTGAACAGACAGAGACCGCCGAAGGCTTCCACGCCATCTGTATCCAGCACGAGA
TCGACCAGCTTGACGGTATCTTCTGGCTGAAGCGCCTCTCCAGGCTCAAGCGCGACCGGCTGGTGAAGAAGTGGGAAAAA
TCCCGAAATCCGTGA

Upstream 100 bases:

>100_bases
AAACGCGACGCCCGTACCCAGCGCAGCTTTCTGGAGGCGACCGGGGAAGGACAGGCGCTCGCCGCCTCATCCTCGCGGAT
TTGAAACGAAGGAGCATTCC

Downstream 100 bases:

>100_bases
CCGTCAGCCGGTTGTCGACAGTGCAAATTCCGCATTGCCCTTCAGCGTGTTGCTGACGGTGCAGATATCCTCCGCCGCAT
GAGCTATTGCGGCGCGGGTC

Product: peptide deformylase

Products: Formate; L-Methionine. [C]

Alternate protein names: Polypeptide deformylase-like

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MAIRPILPYPHAGLSGICAPVTVFDDHLRELVTDLIDTMRAAPGVGITAAHIGVLQRVFVLELTPGTILTYINPEITSHS
PQTMRHVEGSVSMPGFTDEVERPSTVEVRFQDITGAEQTETAEGFHAICIQHEIDQLDGIFWLKRLSRLKRDRLVKKWEK
SRNP

Sequences:

>Translated_164_residues
MAIRPILPYPHAGLSGICAPVTVFDDHLRELVTDLIDTMRAAPGVGITAAHIGVLQRVFVLELTPGTILTYINPEITSHS
PQTMRHVEGSVSMPGFTDEVERPSTVEVRFQDITGAEQTETAEGFHAICIQHEIDQLDGIFWLKRLSRLKRDRLVKKWEK
SRNP
>Mature_163_residues
AIRPILPYPHAGLSGICAPVTVFDDHLRELVTDLIDTMRAAPGVGITAAHIGVLQRVFVLELTPGTILTYINPEITSHSP
QTMRHVEGSVSMPGFTDEVERPSTVEVRFQDITGAEQTETAEGFHAICIQHEIDQLDGIFWLKRLSRLKRDRLVKKWEKS
RNP

Specific function: Removes The Formyl Group From The N-Terminal Met Of Newly Synthesized Proteins. Requires At Least A Dipeptide For An Efficient Rate Of Reaction. N-Terminal L-Methionine Is A Prerequisite For Activity But The Enzyme Has Broad Specificity At Other Positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=162, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24645728, Length=145, Percent_Identity=26.2068965517241, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEFL_AGRT5 (Q8UF49)

Other databases:

- EMBL:   AE007869
- PIR:   AE2767
- PIR:   H97547
- RefSeq:   NP_354552.1
- ProteinModelPortal:   Q8UF49
- SMR:   Q8UF49
- STRING:   Q8UF49
- GeneID:   1133588
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1550
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   DFLAICI
- ProtClustDB:   PRK12846
- BioCyc:   ATUM176299-1:ATU1550-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: 3.5.1.88 [C]

Molecular weight: Translated: 18364; Mature: 18233

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRPILPYPHAGLSGICAPVTVFDDHLRELVTDLIDTMRAAPGVGITAAHIGVLQRVFV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LELTPGTILTYINPEITSHSPQTMRHVEGSVSMPGFTDEVERPSTVEVRFQDITGAEQTE
EEECCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCHHCCCCCEEEEEEECCCCCCCHH
TAEGFHAICIQHEIDQLDGIFWLKRLSRLKRDRLVKKWEKSRNP
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIRPILPYPHAGLSGICAPVTVFDDHLRELVTDLIDTMRAAPGVGITAAHIGVLQRVFV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LELTPGTILTYINPEITSHSPQTMRHVEGSVSMPGFTDEVERPSTVEVRFQDITGAEQTE
EEECCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCHHCCCCCEEEEEEECCCCCCCHH
TAEGFHAICIQHEIDQLDGIFWLKRLSRLKRDRLVKKWEKSRNP
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Formyl-L-Methionine; H2O [C]

Specific reaction: N-Formyl-L-Methionine + H2O = Formate + L-Methionine. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; EDTA; o-Phenantroline [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194