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The map label for this gene is ntrX [H]

Identifier: 15888773

GI number: 15888773

Start: 1443150

End: 1444514

Strand: Direct

Name: ntrX [H]

Synonym: Atu1448

Alternate gene names: 15888773

Gene position: 1443150-1444514 (Clockwise)

Preceding gene: 15888772

Following gene: 15888774

Centisome position: 50.79

GC content: 59.56

Gene sequence:

>1365_bases
ATGGCGTCTGATATTTTGGTCGTGGATGACGAGGCGGACATTCGCGAAATCGTAGCGGGCATCCTGTCCGATGAGGGCCA
CGAGACGCGTATGGCCTTCGACAGCGACAGCGCGCTGGCCGCCATTTCCGAGCGTGTGCCGCGTCTGATCTTTCTCGACA
TCTGGATGCAGGGATCGAAGCTCGACGGTTTGGCACTACTGGACGAGATCAAGAGCCGCCACCCCGAAATTCCTGTCGTC
ATGATTTCCGGTCACGGCAATATCGAAACCGCCGTCAACGCCATCAAGCGCGGGGCCTTCGATTTCATCGAAAAGCCGTT
CAAGGCCGATCGTCTGATCCTGATTGCAGAACGCGCGCTGGAAAATTCCAAGCTGAAGCGCGAGGTCCAGGAACTCAAGA
AGCGTACCGGTGATGCGGTTGAACTCGTCGGCGCGTCGCTCGCGGTTTCGCAACTTCGCCAGACCATTGACAGGGTTGCC
CCCACCAATAGCCGCATCATGATCCTCGGCCCCTCCGGTTCCGGCAAGGAACTGGTGGCGCGCATGGTGCACAAGAAATC
CTCGCGCGCTACCGGACCTTTTGTGGCGCTGAACGCTGCAACGATCACCCCCGACCGTATGGAAATTGCGCTGTTCGGCA
CGGAAGGCTTGCCCGGACAGCCACGCAAGGTAGGGGCTCTGGAAGAAGCCCATCGCGGCGTACTTTATCTCGATGAAGTC
GGTGAAATGCCGCGCGAGACACAAAACAAGATCCTGCGCGTGCTGGTCGATCAGCAGTTCGAGCGTGTCGGCGGCGGCAA
GCGGGTGAAGGTGGATGTGCGGATCATTTCCTCCACCGCCCATCACCTCGAAAGCCTGATCGCTGAAGGCCAGTTCCGGG
AGGATCTCTACCATCGTCTCGCCGTGGTGCCGGTGAAGGTGCCGGCGCTCTCCGAACGGCGCGAGGATATTCCTTTCCTC
GTCGACATGTTCATGCGGCAGATTTCCGAACAGGCCGGCATCCGCCCGCGCAAGATCGGTGATGACGCCATGGCCGTGCT
CCAGACGCATGACTGGCCGGGCAATATCCGCCAGCTGCGCAACAATATCGAGCGGCTGATGATTCTCGCCCGTCCGGAAG
GTGGCGAAGCGCCGATCTCGGCCGACATGCTGCCTTCCGATATTGGCGACATGCTGCCGAAGATCTCTGCGCAGGGCGAT
CAGCACATCATGACCCTGCCGCTGCGTGAAGCCCGTGAAATGTTCGAACGGGACTATCTGATGGCTCAGATCAACCGCTT
CGGTGGCAATATTTCGCGCACGGCGGAATTTGTCGGCATGGAACGGTCGGCGCTGCATCGCAAACTGAAATCTCTCGGCG
TATAA

Upstream 100 bases:

>100_bases
GATGCCCCTGCCGAATTCGACCATGGCCATGGCGCCATGATCCGAGTGCTGCTGCCCTATATCGAGGCGGTCGGCGGCGA
AAATAACAAGGAAGCAGCAT

Downstream 100 bases:

>100_bases
GGAACCAAGGCACCTCGTCGCAACCGATTATTCCGTCAGAGAGACAGCATGAAAGTCATCATTTGCGGCGCAGGGCAGGT
GGGTTACGGCATCGCCGAAC

Product: two component response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MASDILVVDDEADIREIVAGILSDEGHETRMAFDSDSALAAISERVPRLIFLDIWMQGSKLDGLALLDEIKSRHPEIPVV
MISGHGNIETAVNAIKRGAFDFIEKPFKADRLILIAERALENSKLKREVQELKKRTGDAVELVGASLAVSQLRQTIDRVA
PTNSRIMILGPSGSGKELVARMVHKKSSRATGPFVALNAATITPDRMEIALFGTEGLPGQPRKVGALEEAHRGVLYLDEV
GEMPRETQNKILRVLVDQQFERVGGGKRVKVDVRIISSTAHHLESLIAEGQFREDLYHRLAVVPVKVPALSERREDIPFL
VDMFMRQISEQAGIRPRKIGDDAMAVLQTHDWPGNIRQLRNNIERLMILARPEGGEAPISADMLPSDIGDMLPKISAQGD
QHIMTLPLREAREMFERDYLMAQINRFGGNISRTAEFVGMERSALHRKLKSLGV

Sequences:

>Translated_454_residues
MASDILVVDDEADIREIVAGILSDEGHETRMAFDSDSALAAISERVPRLIFLDIWMQGSKLDGLALLDEIKSRHPEIPVV
MISGHGNIETAVNAIKRGAFDFIEKPFKADRLILIAERALENSKLKREVQELKKRTGDAVELVGASLAVSQLRQTIDRVA
PTNSRIMILGPSGSGKELVARMVHKKSSRATGPFVALNAATITPDRMEIALFGTEGLPGQPRKVGALEEAHRGVLYLDEV
GEMPRETQNKILRVLVDQQFERVGGGKRVKVDVRIISSTAHHLESLIAEGQFREDLYHRLAVVPVKVPALSERREDIPFL
VDMFMRQISEQAGIRPRKIGDDAMAVLQTHDWPGNIRQLRNNIERLMILARPEGGEAPISADMLPSDIGDMLPKISAQGD
QHIMTLPLREAREMFERDYLMAQINRFGGNISRTAEFVGMERSALHRKLKSLGV
>Mature_453_residues
ASDILVVDDEADIREIVAGILSDEGHETRMAFDSDSALAAISERVPRLIFLDIWMQGSKLDGLALLDEIKSRHPEIPVVM
ISGHGNIETAVNAIKRGAFDFIEKPFKADRLILIAERALENSKLKREVQELKKRTGDAVELVGASLAVSQLRQTIDRVAP
TNSRIMILGPSGSGKELVARMVHKKSSRATGPFVALNAATITPDRMEIALFGTEGLPGQPRKVGALEEAHRGVLYLDEVG
EMPRETQNKILRVLVDQQFERVGGGKRVKVDVRIISSTAHHLESLIAEGQFREDLYHRLAVVPVKVPALSERREDIPFLV
DMFMRQISEQAGIRPRKIGDDAMAVLQTHDWPGNIRQLRNNIERLMILARPEGGEAPISADMLPSDIGDMLPKISAQGDQ
HIMTLPLREAREMFERDYLMAQINRFGGNISRTAEFVGMERSALHRKLKSLGV

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=35.9574468085106, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1788550, Length=462, Percent_Identity=35.2813852813853, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1790437, Length=450, Percent_Identity=36.4444444444444, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1788905, Length=449, Percent_Identity=33.630289532294, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1789087, Length=431, Percent_Identity=32.7146171693735, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789233, Length=252, Percent_Identity=40.0793650793651, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI1786524, Length=329, Percent_Identity=34.0425531914894, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI87082152, Length=321, Percent_Identity=33.6448598130841, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87082117, Length=323, Percent_Identity=33.4365325077399, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1787583, Length=337, Percent_Identity=33.8278931750742, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=32.7044025157233, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI87081858, Length=289, Percent_Identity=27.3356401384083, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789828, Length=229, Percent_Identity=31.0043668122271, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=40, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50438; Mature: 50307

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDILVVDDEADIREIVAGILSDEGHETRMAFDSDSALAAISERVPRLIFLDIWMQGSK
CCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCCEEEEEEEECCCC
LDGLALLDEIKSRHPEIPVVMISGHGNIETAVNAIKRGAFDFIEKPFKADRLILIAERAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHH
ENSKLKREVQELKKRTGDAVELVGASLAVSQLRQTIDRVAPTNSRIMILGPSGSGKELVA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
RMVHKKSSRATGPFVALNAATITPDRMEIALFGTEGLPGQPRKVGALEEAHRGVLYLDEV
HHHHHHCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHCCCEEEEHHH
GEMPRETQNKILRVLVDQQFERVGGGKRVKVDVRIISSTAHHLESLIAEGQFREDLYHRL
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHH
AVVPVKVPALSERREDIPFLVDMFMRQISEQAGIRPRKIGDDAMAVLQTHDWPGNIRQLR
HEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHH
NNIERLMILARPEGGEAPISADMLPSDIGDMLPKISAQGDQHIMTLPLREAREMFERDYL
HHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHH
MAQINRFGGNISRTAEFVGMERSALHRKLKSLGV
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASDILVVDDEADIREIVAGILSDEGHETRMAFDSDSALAAISERVPRLIFLDIWMQGSK
CCCEEEECCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCCEEEEEEEECCCC
LDGLALLDEIKSRHPEIPVVMISGHGNIETAVNAIKRGAFDFIEKPFKADRLILIAERAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHH
ENSKLKREVQELKKRTGDAVELVGASLAVSQLRQTIDRVAPTNSRIMILGPSGSGKELVA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
RMVHKKSSRATGPFVALNAATITPDRMEIALFGTEGLPGQPRKVGALEEAHRGVLYLDEV
HHHHHHCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHCCCEEEEHHH
GEMPRETQNKILRVLVDQQFERVGGGKRVKVDVRIISSTAHHLESLIAEGQFREDLYHRL
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHH
AVVPVKVPALSERREDIPFLVDMFMRQISEQAGIRPRKIGDDAMAVLQTHDWPGNIRQLR
HEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHH
NNIERLMILARPEGGEAPISADMLPSDIGDMLPKISAQGDQHIMTLPLREAREMFERDYL
HHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHH
MAQINRFGGNISRTAEFVGMERSALHRKLKSLGV
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]