Click here to switch to the map view.

The map label for this gene is pdhB [H]

Identifier: 15888755

GI number: 15888755

Start: 1424710

End: 1426131

Strand: Direct

Name: pdhB [H]

Synonym: Atu1430

Alternate gene names: 15888755

Gene position: 1424710-1426131 (Clockwise)

Preceding gene: 159184757

Following gene: 15888756

Centisome position: 50.14

GC content: 58.93

Gene sequence:

>1422_bases
ATGCCTGTAGAAATTCTTATGCCCGCCCTTTCCCCGACCATGGAGGAAGGCACGCTTTCCAAATGGCTTAAAAAGGAAGG
CGACAAGGTCACCTCCGGCGACGTGATTGCCGAGATCGAGACCGACAAGGCGACCATGGAAGTGGAAGCCGTGGACGAGG
GCGTGATCGGCAAGCTGCTGATCGACGCCGGCACCGAAGGCGTCAAGGTCAATACGCCGATCGCCGTTCTGATCCAGGAA
GGCGAAAGCGCCGACGACATCTCTTCCTCTGCCAAGAAGGAAGAACCGAAGGCAGAAGCTGCAAATTCCGGTTCGGACGC
CGCCGGCGGCAAGACCCGCGAAGCGGCCGAAGAGCCGAGCGCTGCCAAGGAAGCCGCTAAGGTTCCGGCAGCACCCAAGA
TCGAAGTTGCTGCCGATCCCGATATTCCTGAAGGCACGGAAATGGTGATGACGACGGTGCGTGAAGCGCTGCGTGACGCC
ATGGCCGAAGAAATGCGCGCCGACGAAAAAGTCTTCGTCATGGGTGAAGAAGTCGCCGAATATCAGGGCGCCTACAAGAT
CACCCAAGGCCTGTTGCAGGAGTTCGGCGAGCGCCGCGTCATCGATACCCCGATCACCGAGCACGGTTTTGCCGGTATCG
GCGTCGGCGCTGCGATGACGGGCCTCAAGCCCATCGTCGAATTCATGACCTTCAATTTCGCCATGCAGGCGATTGACCAG
ATCGTCAACTCGGCGGCAAAGACGCTTTACATGTCGGGTGGCCAGATGGGTGCACCGATGGTGTTCCGTGGTCCCTCGGG
TGCTGCTGCCCGCGTTGGCGCGCAGCATTCGCAATGTTACGCCGCATGGTACAGCCATATTCCGGGCCTCAAGGTGGTCA
TGCCTTACACGGCAGCCGACGCGAAGGGTCTCTTGAAGGCGGCTATCCGCGATCCGAACCCCGTCATCTTCCTTGAGAAT
GAAATTCTCTATGGCCAGAGCTTCGAAGTGCCGAAGCTCGATGATTTCGTGCTGCCGATCGGCAAGGCGCGCATTCACCG
CAAGGGCAAGGATGCGACCATCGTTTCCTTCGGTATCGGCATGACCTATGCGATCAAGGCGGTTGCGGAACTCGAGAAGC
TCGGCATCGACGTCGAACTGATCGATCTGCGCACCATCCGTCCGATGGACCTGCCCACCGTCATCGAATCGGTCAAGAAG
ACCGGCCGTCTGGTGACCGTCGAGGAAGGTTTCCCGCAGTCATCGGTCGGTGACTTCATCGCCAACCAGGTCATGCGCGC
CGCCTTCGACTATCTCGATGCGCCGATCCTCACGATTGCCGGCAAGGACGTTCCGATGCCTTACGCGGCAAACCTCGAAA
AGTTGGCTCTGCCGAATGTTGACGAAGTCGTTCAGGCTGTCAAAACCGTCTGCTACAAGTAA

Upstream 100 bases:

>100_bases
ATATCGTCGCCGACAGCGCCGACTTCGCCCAGAACGACCCCGAGCCGGATGTATCCGAGCTCTACACCGACATTCTGCTC
TGATCGGGAAAGGGAAACCC

Downstream 100 bases:

>100_bases
GGGGAGGTGAATTTGAGTAAAATAAAACAAATCCTCCCCGCAACTGAAAATTGGTATCGTGTTCTAGGTTCCAAGGCGGC
TCCTCAGTTCGAGCGCGTTG

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MPVEILMPALSPTMEEGTLSKWLKKEGDKVTSGDVIAEIETDKATMEVEAVDEGVIGKLLIDAGTEGVKVNTPIAVLIQE
GESADDISSSAKKEEPKAEAANSGSDAAGGKTREAAEEPSAAKEAAKVPAAPKIEVAADPDIPEGTEMVMTTVREALRDA
MAEEMRADEKVFVMGEEVAEYQGAYKITQGLLQEFGERRVIDTPITEHGFAGIGVGAAMTGLKPIVEFMTFNFAMQAIDQ
IVNSAAKTLYMSGGQMGAPMVFRGPSGAAARVGAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLEN
EILYGQSFEVPKLDDFVLPIGKARIHRKGKDATIVSFGIGMTYAIKAVAELEKLGIDVELIDLRTIRPMDLPTVIESVKK
TGRLVTVEEGFPQSSVGDFIANQVMRAAFDYLDAPILTIAGKDVPMPYAANLEKLALPNVDEVVQAVKTVCYK

Sequences:

>Translated_473_residues
MPVEILMPALSPTMEEGTLSKWLKKEGDKVTSGDVIAEIETDKATMEVEAVDEGVIGKLLIDAGTEGVKVNTPIAVLIQE
GESADDISSSAKKEEPKAEAANSGSDAAGGKTREAAEEPSAAKEAAKVPAAPKIEVAADPDIPEGTEMVMTTVREALRDA
MAEEMRADEKVFVMGEEVAEYQGAYKITQGLLQEFGERRVIDTPITEHGFAGIGVGAAMTGLKPIVEFMTFNFAMQAIDQ
IVNSAAKTLYMSGGQMGAPMVFRGPSGAAARVGAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLEN
EILYGQSFEVPKLDDFVLPIGKARIHRKGKDATIVSFGIGMTYAIKAVAELEKLGIDVELIDLRTIRPMDLPTVIESVKK
TGRLVTVEEGFPQSSVGDFIANQVMRAAFDYLDAPILTIAGKDVPMPYAANLEKLALPNVDEVVQAVKTVCYK
>Mature_472_residues
PVEILMPALSPTMEEGTLSKWLKKEGDKVTSGDVIAEIETDKATMEVEAVDEGVIGKLLIDAGTEGVKVNTPIAVLIQEG
ESADDISSSAKKEEPKAEAANSGSDAAGGKTREAAEEPSAAKEAAKVPAAPKIEVAADPDIPEGTEMVMTTVREALRDAM
AEEMRADEKVFVMGEEVAEYQGAYKITQGLLQEFGERRVIDTPITEHGFAGIGVGAAMTGLKPIVEFMTFNFAMQAIDQI
VNSAAKTLYMSGGQMGAPMVFRGPSGAAARVGAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENE
ILYGQSFEVPKLDDFVLPIGKARIHRKGKDATIVSFGIGMTYAIKAVAELEKLGIDVELIDLRTIRPMDLPTVIESVKKT
GRLVTVEEGFPQSSVGDFIANQVMRAAFDYLDAPILTIAGKDVPMPYAANLEKLALPNVDEVVQAVKTVCYK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=56.7901234567901, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=53.7037037037037, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI4557353, Length=362, Percent_Identity=33.1491712707182, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI34101272, Length=362, Percent_Identity=33.1491712707182, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI203098753, Length=81, Percent_Identity=48.1481481481481, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI203098816, Length=81, Percent_Identity=48.1481481481481, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI31711992, Length=89, Percent_Identity=46.0674157303371, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI260898739, Length=59, Percent_Identity=57.6271186440678, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI133778974, Length=284, Percent_Identity=26.7605633802817, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI225637463, Length=309, Percent_Identity=25.8899676375405, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI225637461, Length=309, Percent_Identity=26.8608414239482, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI225637459, Length=309, Percent_Identity=25.8899676375405, Blast_Score=66, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=321, Percent_Identity=61.3707165109034, Blast_Score=410, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17506935, Length=353, Percent_Identity=39.6600566572238, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17560088, Length=141, Percent_Identity=37.5886524822695, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319698, Length=325, Percent_Identity=59.3846153846154, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324258, Length=145, Percent_Identity=38.6206896551724, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321632, Length=99, Percent_Identity=42.4242424242424, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358145, Length=323, Percent_Identity=57.5851393188854, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24650940, Length=323, Percent_Identity=57.5851393188854, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI160714832, Length=319, Percent_Identity=35.423197492163, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI160714828, Length=319, Percent_Identity=35.423197492163, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24650943, Length=89, Percent_Identity=62.9213483146067, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24650945, Length=89, Percent_Identity=62.9213483146067, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI20129315, Length=76, Percent_Identity=48.6842105263158, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24582497, Length=65, Percent_Identity=47.6923076923077, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45551847, Length=242, Percent_Identity=26.8595041322314, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45550715, Length=242, Percent_Identity=26.8595041322314, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24645119, Length=242, Percent_Identity=26.8595041322314, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 50596; Mature: 50464

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVEILMPALSPTMEEGTLSKWLKKEGDKVTSGDVIAEIETDKATMEVEAVDEGVIGKLL
CCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHE
IDAGTEGVKVNTPIAVLIQEGESADDISSSAKKEEPKAEAANSGSDAAGGKTREAAEEPS
EECCCCCEEECCCEEEEEECCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHCCCH
AAKEAAKVPAAPKIEVAADPDIPEGTEMVMTTVREALRDAMAEEMRADEKVFVMGEEVAE
HHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHH
YQGAYKITQGLLQEFGERRVIDTPITEHGFAGIGVGAAMTGLKPIVEFMTFNFAMQAIDQ
HCCHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IVNSAAKTLYMSGGQMGAPMVFRGPSGAAARVGAQHSQCYAAWYSHIPGLKVVMPYTAAD
HHHHHHHHEEECCCCCCCCEEEECCCCCHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCC
AKGLLKAAIRDPNPVIFLENEILYGQSFEVPKLDDFVLPIGKARIHRKGKDATIVSFGIG
HHHHHHHHHCCCCCEEEEECEEEECCCCCCCCCCCHHCCCCHHHHHCCCCCCEEEEECCC
MTYAIKAVAELEKLGIDVELIDLRTIRPMDLPTVIESVKKTGRLVTVEEGFPQSSVGDFI
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
ANQVMRAAFDYLDAPILTIAGKDVPMPYAANLEKLALPNVDEVVQAVKTVCYK
HHHHHHHHHHHHCCCEEEECCCCCCCCHHCCCHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVEILMPALSPTMEEGTLSKWLKKEGDKVTSGDVIAEIETDKATMEVEAVDEGVIGKLL
CCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHE
IDAGTEGVKVNTPIAVLIQEGESADDISSSAKKEEPKAEAANSGSDAAGGKTREAAEEPS
EECCCCCEEECCCEEEEEECCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHHCCCH
AAKEAAKVPAAPKIEVAADPDIPEGTEMVMTTVREALRDAMAEEMRADEKVFVMGEEVAE
HHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHH
YQGAYKITQGLLQEFGERRVIDTPITEHGFAGIGVGAAMTGLKPIVEFMTFNFAMQAIDQ
HCCHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IVNSAAKTLYMSGGQMGAPMVFRGPSGAAARVGAQHSQCYAAWYSHIPGLKVVMPYTAAD
HHHHHHHHEEECCCCCCCCEEEECCCCCHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCC
AKGLLKAAIRDPNPVIFLENEILYGQSFEVPKLDDFVLPIGKARIHRKGKDATIVSFGIG
HHHHHHHHHCCCCCEEEEECEEEECCCCCCCCCCCHHCCCCHHHHHCCCCCCEEEEECCC
MTYAIKAVAELEKLGIDVELIDLRTIRPMDLPTVIESVKKTGRLVTVEEGFPQSSVGDFI
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
ANQVMRAAFDYLDAPILTIAGKDVPMPYAANLEKLALPNVDEVVQAVKTVCYK
HHHHHHHHHHHHCCCEEEECCCCCCCCHHCCCHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]