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The map label for this gene is nuoI

Identifier: 15888607

GI number: 15888607

Start: 1265430

End: 1265921

Strand: Direct

Name: nuoI

Synonym: Atu1278

Alternate gene names: 15888607

Gene position: 1265430-1265921 (Clockwise)

Preceding gene: 15888606

Following gene: 15888608

Centisome position: 44.53

GC content: 58.13

Gene sequence:

>492_bases
ATGGCAAGCCTCTCCCAAGCCGTCAACTCGCTGTTCCTGAAGGAATTCGTAGGCGCGATCTTCCTGACCATGCGCCATTT
CTTCAAGCAGAAGGCGACGATCAATTATCCGTTTGAAAAGGGTCCGGTGTCTCCGCGCTTCCGCGGCGAACATGCGTTGC
GCCGTTATCCGAACGGTGAAGAACGCTGCATCGCCTGCAAGCTGTGTGAAGCGATCTGTCCTGCTCAGGCGATCACCATC
GAAGCCGGCCCGCGCCGCAATGACGGCACCCGCCGCACGGTGCGTTACGACATCGACATGGTGAAGTGCATCTATTGCGG
TTTCTGCCAGGAAGCCTGCCCGGTGGATGCAATCGTCGAGGGTCCGAACTTCGAATTCGCGACGGAAACCCGCGAGGAGC
TTTATTTCGACAAGCAGAGGCTTCTCGACAATGGCGACCGCTGGGAGCGTGAAATCGCACGCAACCTCGCACTGGATGCA
CCTTATCGCTGA

Upstream 100 bases:

>100_bases
GCTGTCGCTCGCCATGGTCGTTATTGTTGCATTCGTATTGAAGCTGACGGGGTGGGCCGGTTGATGTCTGTATTCCCCGC
GCAGAAATCTGGAGACTGAG

Downstream 100 bases:

>100_bases
GGCGCAACCGTCTTTCCGGGGGAGACCCCGGAAAGCGATGAAAAAGTAAAATGCCTGCGCGGTTTCCGCCGGCGGGGACG
AAACGGGTGAGGGGCCTTCG

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MASLSQAVNSLFLKEFVGAIFLTMRHFFKQKATINYPFEKGPVSPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITI
EAGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEFATETREELYFDKQRLLDNGDRWEREIARNLALDA
PYR

Sequences:

>Translated_163_residues
MASLSQAVNSLFLKEFVGAIFLTMRHFFKQKATINYPFEKGPVSPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITI
EAGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEFATETREELYFDKQRLLDNGDRWEREIARNLALDA
PYR
>Mature_162_residues
ASLSQAVNSLFLKEFVGAIFLTMRHFFKQKATINYPFEKGPVSPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITIE
AGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEFATETREELYFDKQRLLDNGDRWEREIARNLALDAP
YR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=158, Percent_Identity=71.5189873417722, Blast_Score=243, Evalue=4e-65,
Organism=Escherichia coli, GI1788617, Length=129, Percent_Identity=38.7596899224806, Blast_Score=100, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17555194, Length=162, Percent_Identity=67.2839506172839, Blast_Score=232, Evalue=7e-62,
Organism=Drosophila melanogaster, GI17864306, Length=158, Percent_Identity=72.7848101265823, Blast_Score=246, Evalue=4e-66,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_AGRT5 (Q8UFW9)

Other databases:

- EMBL:   AE007869
- PIR:   AF2733
- PIR:   H97514
- RefSeq:   NP_354288.1
- HSSP:   P00198
- ProteinModelPortal:   Q8UFW9
- SMR:   Q8UFW9
- STRING:   Q8UFW9
- GeneID:   1133316
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1278
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   EMVRGLM
- PhylomeDB:   Q8UFW9
- ProtClustDB:   PRK05888
- BioCyc:   ATUM176299-1:ATU1278-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 18796; Mature: 18665

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLSQAVNSLFLKEFVGAIFLTMRHFFKQKATINYPFEKGPVSPRFRGEHALRRYPNGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
ERCIACKLCEAICPAQAITIEAGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVE
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEC
GPNFEFATETREELYFDKQRLLDNGDRWEREIARNLALDAPYR
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ASLSQAVNSLFLKEFVGAIFLTMRHFFKQKATINYPFEKGPVSPRFRGEHALRRYPNGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
ERCIACKLCEAICPAQAITIEAGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVE
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEC
GPNFEFATETREELYFDKQRLLDNGDRWEREIARNLALDAPYR
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194