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The map label for this gene is nikB [H]

Identifier: 15888534

GI number: 15888534

Start: 1190587

End: 1191531

Strand: Direct

Name: nikB [H]

Synonym: Atu1198

Alternate gene names: 15888534

Gene position: 1190587-1191531 (Clockwise)

Preceding gene: 15888533

Following gene: 15888535

Centisome position: 41.9

GC content: 61.8

Gene sequence:

>945_bases
ATGTGGATACAGATAATACAGCGCTTCTTTATTCTGTGCCTGATGCTGGCCATTGTCTCCATCATCGCGTTTCTGTTGCC
CTATATGACGGGCGGGGATCCGGCACGCACCATCCTCTTCTCGCGCATGCACGATACCGCGCTCGATCCGCACGCGGTGG
AGGCGTTGAGGGTCAGTCTTGGGCTCGACCGGCCGCTTTACGTGCAATATTTCACATGGCTGTGGAATGCACTGCGCGGT
GATCTCGGTTTTTCTTTCACCAGCAGTCAGCCTGTCGCCAACGAGCTTTTGCGCTCTCTCGGCGTATCAGTGACCCTGGC
GCTGACGGCATTGGCCGTCGCGGTCGCCGTGGCGCTGCCGCTCGGCACGCTTGCGGCCATGCGCCCGGGTGGACGGCTCG
ACAATTTCGCGACACTGATGATCCAGACCTTCGTCGCGACACCGGAATACTGGTTTGCGCCCATGTCGGCGCTGGTCTTT
GCGCTTTATCTCGGCTGGTTGCCCTCGGCCGGATGGGACAGCTGGCGTTCGCTGGTGCTGCCTGCGCTGACCCTGACACT
GCGGCCGCTCGCCTATTTCACCCAGGTCACCCGTGCCGCCATGGCCGAGGTCTTGCGCGCGCCCTATATCACGGCGGCAC
GCAGCCGCGGCCTTGGCATGCATGGCACGGTCATGCGCCACGGCATCCGCAACGGCTCGCTGCCGGTGGTCACCTTCTTT
GCGCTGTGGCTTGCGGGCTTGCTTGGCGGATCGGTGGTGGTGGAAGTGATCTTCGCCATTCCCGGCATGGGCCGGCTCCT
CTACGACGCCGTGATCAATCGCGACATCCCGATGCTGCAGGGCGGTTTCATCTGCATCGTGGCCCTGTCCATCCTGGTCA
ATACCCTGGCCGACAGCTTCTATGTTCTGATCAATCCAGCCATGCGAGGTCAACATGACCACTAG

Upstream 100 bases:

>100_bases
TGGATTTACAGCGCCAGGCGAAATAAGCCGCCGGTTCCCTTTTGGGGAGCGGGCACCGAATGCAGTGTAAACTGCCAATC
TTGATGAAGGAGAGCAGGCC

Downstream 100 bases:

>100_bases
TCTTTTCCCGAGCGCGCCTGCCGCCGCCATGCCCATGACCTTGCCCACACGCCCGTCGACACTCTCCCGGTTCACGGATT
TCATCCGCCGGCGGCACTGG

Product: ABC transporter, membrane spanning protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MWIQIIQRFFILCLMLAIVSIIAFLLPYMTGGDPARTILFSRMHDTALDPHAVEALRVSLGLDRPLYVQYFTWLWNALRG
DLGFSFTSSQPVANELLRSLGVSVTLALTALAVAVAVALPLGTLAAMRPGGRLDNFATLMIQTFVATPEYWFAPMSALVF
ALYLGWLPSAGWDSWRSLVLPALTLTLRPLAYFTQVTRAAMAEVLRAPYITAARSRGLGMHGTVMRHGIRNGSLPVVTFF
ALWLAGLLGGSVVVEVIFAIPGMGRLLYDAVINRDIPMLQGGFICIVALSILVNTLADSFYVLINPAMRGQHDH

Sequences:

>Translated_314_residues
MWIQIIQRFFILCLMLAIVSIIAFLLPYMTGGDPARTILFSRMHDTALDPHAVEALRVSLGLDRPLYVQYFTWLWNALRG
DLGFSFTSSQPVANELLRSLGVSVTLALTALAVAVAVALPLGTLAAMRPGGRLDNFATLMIQTFVATPEYWFAPMSALVF
ALYLGWLPSAGWDSWRSLVLPALTLTLRPLAYFTQVTRAAMAEVLRAPYITAARSRGLGMHGTVMRHGIRNGSLPVVTFF
ALWLAGLLGGSVVVEVIFAIPGMGRLLYDAVINRDIPMLQGGFICIVALSILVNTLADSFYVLINPAMRGQHDH
>Mature_314_residues
MWIQIIQRFFILCLMLAIVSIIAFLLPYMTGGDPARTILFSRMHDTALDPHAVEALRVSLGLDRPLYVQYFTWLWNALRG
DLGFSFTSSQPVANELLRSLGVSVTLALTALAVAVAVALPLGTLAAMRPGGRLDNFATLMIQTFVATPEYWFAPMSALVF
ALYLGWLPSAGWDSWRSLVLPALTLTLRPLAYFTQVTRAAMAEVLRAPYITAARSRGLGMHGTVMRHGIRNGSLPVVTFF
ALWLAGLLGGSVVVEVIFAIPGMGRLLYDAVINRDIPMLQGGFICIVALSILVNTLADSFYVLINPAMRGQHDH

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=310, Percent_Identity=37.0967741935484, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1787053, Length=305, Percent_Identity=37.3770491803279, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1789965, Length=325, Percent_Identity=31.6923076923077, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787497, Length=268, Percent_Identity=31.7164179104478, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787761, Length=318, Percent_Identity=32.0754716981132, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1788504, Length=278, Percent_Identity=29.8561151079137, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787550, Length=277, Percent_Identity=25.9927797833935, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34396; Mature: 34396

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWIQIIQRFFILCLMLAIVSIIAFLLPYMTGGDPARTILFSRMHDTALDPHAVEALRVSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
GLDRPLYVQYFTWLWNALRGDLGFSFTSSQPVANELLRSLGVSVTLALTALAVAVAVALP
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LGTLAAMRPGGRLDNFATLMIQTFVATPEYWFAPMSALVFALYLGWLPSAGWDSWRSLVL
HHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
PALTLTLRPLAYFTQVTRAAMAEVLRAPYITAARSRGLGMHGTVMRHGIRNGSLPVVTFF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHH
ALWLAGLLGGSVVVEVIFAIPGMGRLLYDAVINRDIPMLQGGFICIVALSILVNTLADSF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHH
YVLINPAMRGQHDH
EEEECHHHCCCCCC
>Mature Secondary Structure
MWIQIIQRFFILCLMLAIVSIIAFLLPYMTGGDPARTILFSRMHDTALDPHAVEALRVSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
GLDRPLYVQYFTWLWNALRGDLGFSFTSSQPVANELLRSLGVSVTLALTALAVAVAVALP
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LGTLAAMRPGGRLDNFATLMIQTFVATPEYWFAPMSALVFALYLGWLPSAGWDSWRSLVL
HHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
PALTLTLRPLAYFTQVTRAAMAEVLRAPYITAARSRGLGMHGTVMRHGIRNGSLPVVTFF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHH
ALWLAGLLGGSVVVEVIFAIPGMGRLLYDAVINRDIPMLQGGFICIVALSILVNTLADSF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHH
YVLINPAMRGQHDH
EEEECHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]