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The map label for this gene is ribH

Identifier: 15888508

GI number: 15888508

Start: 1164851

End: 1165297

Strand: Direct

Name: ribH

Synonym: Atu1171

Alternate gene names: 15888508

Gene position: 1164851-1165297 (Clockwise)

Preceding gene: 15888507

Following gene: 159184647

Centisome position: 40.99

GC content: 60.18

Gene sequence:

>447_bases
ATGTCCAAACCCCATCTTCTTATCGTGGAAGCACGTTTTTACGACGATATGGCGGATGCCCTTCTCGAAGGCGCGAAGTT
CGCGCTTGAGGAAGCCGGCGCCACCTATGACGTCATCACCGTTCCCGGCGCGCTGGAAATCCCCGCCGCTATCGCCATGG
CGCTCGATGGTGCAGACAATGACGGCACCGAATATGACGGTTTCGTTGCGCTCGGCATGGTCATTCGCGGCGAAACCTAT
CACTTCGATATCGTCTCCAACGAATCCTCCCGCGCCCTGATGGATCTCGCCGTCAGCGAATCGCTGCCGATCGGCAACGG
CATCCTCACCGTCGAAAATGACGAGCAGGCCTGGGCGCGCGTGCGCCGCTCCGACAAGGACAAGGGCGGTTTTGCCGCCC
GCGCGGCGCTCACCATGATCGAGCTGAAAAAGAAACTGGGTGGCTGA

Upstream 100 bases:

>100_bases
GAAAATACTTGCCAAGAACGGCCAGCCGTGGTTTGACCGCCGCAAATGCCGGTATGATCCGGCGTAAAGCTTTTCGGGAA
AATCCCCCAGGAACCCTCAC

Downstream 100 bases:

>100_bases
ACGCATGTCGGATGTTGAAAACGGCGGCGAACCGCGCCAGCCTTCGGTAAAGCCCGCCAACCAGCGCGGTGCGGCCCGTC
TAGCGGCCGTGCAGGCGCTG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MSKPHLLIVEARFYDDMADALLEGAKFALEEAGATYDVITVPGALEIPAAIAMALDGADNDGTEYDGFVALGMVIRGETY
HFDIVSNESSRALMDLAVSESLPIGNGILTVENDEQAWARVRRSDKDKGGFAARAALTMIELKKKLGG

Sequences:

>Translated_148_residues
MSKPHLLIVEARFYDDMADALLEGAKFALEEAGATYDVITVPGALEIPAAIAMALDGADNDGTEYDGFVALGMVIRGETY
HFDIVSNESSRALMDLAVSESLPIGNGILTVENDEQAWARVRRSDKDKGGFAARAALTMIELKKKLGG
>Mature_147_residues
SKPHLLIVEARFYDDMADALLEGAKFALEEAGATYDVITVPGALEIPAAIAMALDGADNDGTEYDGFVALGMVIRGETYH
FDIVSNESSRALMDLAVSESLPIGNGILTVENDEQAWARVRRSDKDKGGFAARAALTMIELKKKLGG

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=144, Percent_Identity=41.6666666666667, Blast_Score=86, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6324429, Length=122, Percent_Identity=33.6065573770492, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_AGRT5 (Q8UG70)

Other databases:

- EMBL:   AE007869
- PIR:   AI2720
- PIR:   E97502
- RefSeq:   NP_354189.1
- ProteinModelPortal:   Q8UG70
- SMR:   Q8UG70
- STRING:   Q8UG70
- GeneID:   1133209
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1171
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   SSRALMD
- ProtClustDB:   PRK00061
- BioCyc:   ATUM176299-1:ATU1171-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 15850; Mature: 15719

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPHLLIVEARFYDDMADALLEGAKFALEEAGATYDVITVPGALEIPAAIAMALDGADN
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHEEEEECCCCC
DGTEYDGFVALGMVIRGETYHFDIVSNESSRALMDLAVSESLPIGNGILTVENDEQAWAR
CCCCCCCEEEEEEEEECCEEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHH
VRRSDKDKGGFAARAALTMIELKKKLGG
HHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKPHLLIVEARFYDDMADALLEGAKFALEEAGATYDVITVPGALEIPAAIAMALDGADN
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHEEEEECCCCC
DGTEYDGFVALGMVIRGETYHFDIVSNESSRALMDLAVSESLPIGNGILTVENDEQAWAR
CCCCCCCEEEEEEEEECCEEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHH
VRRSDKDKGGFAARAALTMIELKKKLGG
HHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194