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The map label for this gene is nwsB [H]

Identifier: 15888455

GI number: 15888455

Start: 1108103

End: 1108711

Strand: Direct

Name: nwsB [H]

Synonym: Atu1116

Alternate gene names: 15888455

Gene position: 1108103-1108711 (Clockwise)

Preceding gene: 15888454

Following gene: 15888456

Centisome position: 39.0

GC content: 60.26

Gene sequence:

>609_bases
ATGACGCTGCCCGTCTATCTCGTTGATGACGATGACGCCGTGCGCAAGGCGCTGATATTGCTCCTGTCCACCGTAGGCAT
CAAGGCGAAGGGCTTTGCCGATCCGGTGGTTTTTCTCGGTCAATTGCCCGCTCTCGAGCCGGGCTGCCTCATTTTCGATA
TCCGCATGCCTGCCATCACCGGATTGAAGCTGCAGGAAAAGCTGGCGGAGGAGGGTGTCGACTGGCCGGTCATCGTCATT
TCCGGCCATGGCAATATCGAAGCCTGCCGCCGTGCCTTTCACAATGGCGCAGTGGATTTTCTCTCCAAGCCCGTAGATGA
GCAGGATCTTATCGATGCGATCCAGAAGGCGCACCGGATATTGGCCAGCACGCTCGACGCAAAAGCGCTGCATGCCGAGA
CGAGGGCGCTTTTAGCGCTGCTGACCGCACGTGAGCGTGAAATCCTCGAACGTATCGCACTCGGTTTCACCTCGCGCCAG
ATCGCCGATGGGCTTGGCCTGTCGCCGCGCACGGTGGACAGCCATCGCGCCGCTATCGGCCTCAAGCTCGGCACCACCTC
GCAGGCAGAAATGACGCGGTTGTGGCTGGAAGCCGGCGCGGATCAGTAA

Upstream 100 bases:

>100_bases
GACTGGTGGAGCGCGCCGGCGGTGAAATTCTCTACGTCGACAGTGAGAATGGCGCGCTTTTCCGCGTCATCTTTCCTGTC
GCGGCGGAGGCGCGGAACCC

Downstream 100 bases:

>100_bases
AACTACCGAGCAGCCGGCAAATCTTACGGATTCGCAGGTTCGCGTCGTCCGCCTAATCTGGGGTCCTCATCAAACAAGAG
GACCTTTTCATGATCCGCAC

Product: two component response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MTLPVYLVDDDDAVRKALILLLSTVGIKAKGFADPVVFLGQLPALEPGCLIFDIRMPAITGLKLQEKLAEEGVDWPVIVI
SGHGNIEACRRAFHNGAVDFLSKPVDEQDLIDAIQKAHRILASTLDAKALHAETRALLALLTAREREILERIALGFTSRQ
IADGLGLSPRTVDSHRAAIGLKLGTTSQAEMTRLWLEAGADQ

Sequences:

>Translated_202_residues
MTLPVYLVDDDDAVRKALILLLSTVGIKAKGFADPVVFLGQLPALEPGCLIFDIRMPAITGLKLQEKLAEEGVDWPVIVI
SGHGNIEACRRAFHNGAVDFLSKPVDEQDLIDAIQKAHRILASTLDAKALHAETRALLALLTAREREILERIALGFTSRQ
IADGLGLSPRTVDSHRAAIGLKLGTTSQAEMTRLWLEAGADQ
>Mature_201_residues
TLPVYLVDDDDAVRKALILLLSTVGIKAKGFADPVVFLGQLPALEPGCLIFDIRMPAITGLKLQEKLAEEGVDWPVIVIS
GHGNIEACRRAFHNGAVDFLSKPVDEQDLIDAIQKAHRILASTLDAKALHAETRALLALLTAREREILERIALGFTSRQI
ADGLGLSPRTVDSHRAAIGLKLGTTSQAEMTRLWLEAGADQ

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=31.858407079646, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788222, Length=209, Percent_Identity=28.7081339712919, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1790102, Length=193, Percent_Identity=34.1968911917098, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1788550, Length=127, Percent_Identity=25.9842519685039, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 21879; Mature: 21747

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPVYLVDDDDAVRKALILLLSTVGIKAKGFADPVVFLGQLPALEPGCLIFDIRMPAIT
CCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCC
GLKLQEKLAEEGVDWPVIVISGHGNIEACRRAFHNGAVDFLSKPVDEQDLIDAIQKAHRI
CHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHH
LASTLDAKALHAETRALLALLTAREREILERIALGFTSRQIADGLGLSPRTVDSHRAAIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHEEE
LKLGTTSQAEMTRLWLEAGADQ
EEECCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TLPVYLVDDDDAVRKALILLLSTVGIKAKGFADPVVFLGQLPALEPGCLIFDIRMPAIT
CEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCC
GLKLQEKLAEEGVDWPVIVISGHGNIEACRRAFHNGAVDFLSKPVDEQDLIDAIQKAHRI
CHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHH
LASTLDAKALHAETRALLALLTAREREILERIALGFTSRQIADGLGLSPRTVDSHRAAIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHEEE
LKLGTTSQAEMTRLWLEAGADQ
EEECCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]