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The map label for this gene is fixL [H]

Identifier: 15888454

GI number: 15888454

Start: 1106445

End: 1108106

Strand: Direct

Name: fixL [H]

Synonym: Atu1115

Alternate gene names: 15888454

Gene position: 1106445-1108106 (Clockwise)

Preceding gene: 159184620

Following gene: 15888455

Centisome position: 38.94

GC content: 62.27

Gene sequence:

>1662_bases
ATGTTCCTTGACGAACCGATGGTGCGACCGCTTTTTCCGGTCACGCGAGTGAGCCAATGCCTGAAAATGACGATGCCTGA
AAACGACGATGCATGGAGTGACATGCCCGGAAAACCGGCTCAGCCCTTGCCGGACGATCCGCGCCCGCAGGCAATATGGT
CCTTGCCAGGGGTGATTCTGGTCTGGGCGCTGCTGATGGCGGCCGTCATTTCTGCACTGGCGGCTTACCAATATGTCAGC
TTGCTCGGCGAGCTGGAGCGGCAGAGCGACGCATTGCAGGCCGAGGTGTCCCGCCGGGCCGACCAGCATGACGCCCATGT
CACCGCGCTTTCGGCGGTGGCGCAGGCCGAGCAGGGCGCGGATTACGGTTTGCTGCTCGACGTTGCCGCTCCCATTCTGC
AGTTTTATCCGCGCATTGATGAGCTTCAGCTGGTGTCCCTCAATGAGGGAGGCCCCGTGGTTGGCACCCGGCCATTGGAA
AACGAGCTGGCGATAGTCATCCGCAAGGCGGCATTGGTGTTTTCCGGCCACCCGGTTCTGGTGGCAAGCGCTCTCCGGCC
CGGGCATTACATGATCGTCAAGCGCAGTCCCAACAGCGAGACGGCGTCGAAGGTTCTGGTGCTCGCAATCGACGCGGCGC
GACTTCTGGCATCCGATGATCCTTTCTGGTCGACGAAACAGGCGGTGATCCGTCTGAAAATGCCGGATGGCACGATATTG
TATGGTCCACCGACATTGCCGGAAAAGCCGCAATATGTCCGGCAGTTGAGCAGCGCCACCCAGCCGCTGCTTCTCGAAGC
CGCTCTTTCGATCACCTGGCGGGATCTTCTGCCGGGCCGCGCCCTGTTGGCGGGAATGGTCGGCGCTACCATGGTTTTCC
TGCTGGGCATCATGATGGCGAAACAGCGGACGCGCATGCGTGCCGCCGAAAGACGGGCCGAACTGAGCGGCATGGAAGCA
AGGCTTACCCATGCCTCGCGGGTCAATGCGCTGGGTGAGATGGCGAGCGGTCTGGCGCATGAGCTGACCCAGCCTTTGAC
GGCGATATTGGCGCAGGCGCAGGCCGGGCGACGGTTGCTTGCCCGCCAGGATACGGTGGCCCTTTCCGGCGCTCTGGATG
ACATGATCGATCAGGCGCGAAGTGCGGCCAACATGCTCGTCCGGTTTCGCAATTGGTCGCTGCCGCACCGTCAACCGGCC
GCCGCGCATGATCTGCGCGCCGCCTTGCGCAATGTCGATGCGCTGCTTGCCGGTGAGGCGGCACGGCACGCGGCCAGCAT
CGATATCGCCATGCCGGAGACGCCGCTTTCGGTGCGGGCCGATCCTGTGGAAATGGAGCAGGTGATGTTCAATCTGCTGC
GTAATGCTCTTGATGCGCCCACGGACGTCGAAGGCAGGAAAGCCATCACCGCCAGATTGAGCCGAAAAGGTGATTTCGCC
GTGTTCGAGGTGGCCGATAATGGCCCCGGCGTCGCACCGGATTTGCGCGACCGCCTGTTTACGCCTTTCGTAACCACGAA
AAAGGACGGCATGGGTCTGGGGCTTGCCCTCAGCCAAAGACTGGTGGAGCGCGCCGGCGGTGAAATTCTCTACGTCGACA
GTGAGAATGGCGCGCTTTTCCGCGTCATCTTTCCTGTCGCGGCGGAGGCGCGGAACCCATGA

Upstream 100 bases:

>100_bases
CCGGGCATACGCCTAGCGCGTCGGGAACCTCACCGGCCAATGGCGAGGGCATGAATTCCGTCGTAGCCGGATTGAATGTT
TCCCCGGTCTGTGCTGAGGA

Downstream 100 bases:

>100_bases
CGCTGCCCGTCTATCTCGTTGATGACGATGACGCCGTGCGCAAGGCGCTGATATTGCTCCTGTCCACCGTAGGCATCAAG
GCGAAGGGCTTTGCCGATCC

Product: two component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MFLDEPMVRPLFPVTRVSQCLKMTMPENDDAWSDMPGKPAQPLPDDPRPQAIWSLPGVILVWALLMAAVISALAAYQYVS
LLGELERQSDALQAEVSRRADQHDAHVTALSAVAQAEQGADYGLLLDVAAPILQFYPRIDELQLVSLNEGGPVVGTRPLE
NELAIVIRKAALVFSGHPVLVASALRPGHYMIVKRSPNSETASKVLVLAIDAARLLASDDPFWSTKQAVIRLKMPDGTIL
YGPPTLPEKPQYVRQLSSATQPLLLEAALSITWRDLLPGRALLAGMVGATMVFLLGIMMAKQRTRMRAAERRAELSGMEA
RLTHASRVNALGEMASGLAHELTQPLTAILAQAQAGRRLLARQDTVALSGALDDMIDQARSAANMLVRFRNWSLPHRQPA
AAHDLRAALRNVDALLAGEAARHAASIDIAMPETPLSVRADPVEMEQVMFNLLRNALDAPTDVEGRKAITARLSRKGDFA
VFEVADNGPGVAPDLRDRLFTPFVTTKKDGMGLGLALSQRLVERAGGEILYVDSENGALFRVIFPVAAEARNP

Sequences:

>Translated_553_residues
MFLDEPMVRPLFPVTRVSQCLKMTMPENDDAWSDMPGKPAQPLPDDPRPQAIWSLPGVILVWALLMAAVISALAAYQYVS
LLGELERQSDALQAEVSRRADQHDAHVTALSAVAQAEQGADYGLLLDVAAPILQFYPRIDELQLVSLNEGGPVVGTRPLE
NELAIVIRKAALVFSGHPVLVASALRPGHYMIVKRSPNSETASKVLVLAIDAARLLASDDPFWSTKQAVIRLKMPDGTIL
YGPPTLPEKPQYVRQLSSATQPLLLEAALSITWRDLLPGRALLAGMVGATMVFLLGIMMAKQRTRMRAAERRAELSGMEA
RLTHASRVNALGEMASGLAHELTQPLTAILAQAQAGRRLLARQDTVALSGALDDMIDQARSAANMLVRFRNWSLPHRQPA
AAHDLRAALRNVDALLAGEAARHAASIDIAMPETPLSVRADPVEMEQVMFNLLRNALDAPTDVEGRKAITARLSRKGDFA
VFEVADNGPGVAPDLRDRLFTPFVTTKKDGMGLGLALSQRLVERAGGEILYVDSENGALFRVIFPVAAEARNP
>Mature_553_residues
MFLDEPMVRPLFPVTRVSQCLKMTMPENDDAWSDMPGKPAQPLPDDPRPQAIWSLPGVILVWALLMAAVISALAAYQYVS
LLGELERQSDALQAEVSRRADQHDAHVTALSAVAQAEQGADYGLLLDVAAPILQFYPRIDELQLVSLNEGGPVVGTRPLE
NELAIVIRKAALVFSGHPVLVASALRPGHYMIVKRSPNSETASKVLVLAIDAARLLASDDPFWSTKQAVIRLKMPDGTIL
YGPPTLPEKPQYVRQLSSATQPLLLEAALSITWRDLLPGRALLAGMVGATMVFLLGIMMAKQRTRMRAAERRAELSGMEA
RLTHASRVNALGEMASGLAHELTQPLTAILAQAQAGRRLLARQDTVALSGALDDMIDQARSAANMLVRFRNWSLPHRQPA
AAHDLRAALRNVDALLAGEAARHAASIDIAMPETPLSVRADPVEMEQVMFNLLRNALDAPTDVEGRKAITARLSRKGDFA
VFEVADNGPGVAPDLRDRLFTPFVTTKKDGMGLGLALSQRLVERAGGEILYVDSENGALFRVIFPVAAEARNP

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=28.1512605042017, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1790300, Length=237, Percent_Identity=28.6919831223629, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788549, Length=271, Percent_Identity=30.9963099630996, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789808, Length=220, Percent_Identity=28.6363636363636, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1786912, Length=273, Percent_Identity=25.2747252747253, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1786837, Length=154, Percent_Identity=38.3116883116883, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=220, Percent_Identity=29.0909090909091, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87081816, Length=227, Percent_Identity=31.7180616740088, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1790346, Length=226, Percent_Identity=28.7610619469027, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59987; Mature: 59987

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLDEPMVRPLFPVTRVSQCLKMTMPENDDAWSDMPGKPAQPLPDDPRPQAIWSLPGVIL
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHH
VWALLMAAVISALAAYQYVSLLGELERQSDALQAEVSRRADQHDAHVTALSAVAQAEQGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCC
DYGLLLDVAAPILQFYPRIDELQLVSLNEGGPVVGTRPLENELAIVIRKAALVFSGHPVL
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCE
VASALRPGHYMIVKRSPNSETASKVLVLAIDAARLLASDDPFWSTKQAVIRLKMPDGTIL
EEECCCCCEEEEEEECCCCHHHHEEEEEEEHHHHHHCCCCCCCCCCEEEEEEECCCCEEE
YGPPTLPEKPQYVRQLSSATQPLLLEAALSITWRDLLPGRALLAGMVGATMVFLLGIMMA
ECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
KQRTRMRAAERRAELSGMEARLTHASRVNALGEMASGLAHELTQPLTAILAQAQAGRRLL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARQDTVALSGALDDMIDQARSAANMLVRFRNWSLPHRQPAAAHDLRAALRNVDALLAGEA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARHAASIDIAMPETPLSVRADPVEMEQVMFNLLRNALDAPTDVEGRKAITARLSRKGDFA
HHHHCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEE
VFEVADNGPGVAPDLRDRLFTPFVTTKKDGMGLGLALSQRLVERAGGEILYVDSENGALF
EEEEECCCCCCCCHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEE
RVIFPVAAEARNP
EEEEHHHHCCCCC
>Mature Secondary Structure
MFLDEPMVRPLFPVTRVSQCLKMTMPENDDAWSDMPGKPAQPLPDDPRPQAIWSLPGVIL
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHH
VWALLMAAVISALAAYQYVSLLGELERQSDALQAEVSRRADQHDAHVTALSAVAQAEQGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCC
DYGLLLDVAAPILQFYPRIDELQLVSLNEGGPVVGTRPLENELAIVIRKAALVFSGHPVL
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCE
VASALRPGHYMIVKRSPNSETASKVLVLAIDAARLLASDDPFWSTKQAVIRLKMPDGTIL
EEECCCCCEEEEEEECCCCHHHHEEEEEEEHHHHHHCCCCCCCCCCEEEEEEECCCCEEE
YGPPTLPEKPQYVRQLSSATQPLLLEAALSITWRDLLPGRALLAGMVGATMVFLLGIMMA
ECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
KQRTRMRAAERRAELSGMEARLTHASRVNALGEMASGLAHELTQPLTAILAQAQAGRRLL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARQDTVALSGALDDMIDQARSAANMLVRFRNWSLPHRQPAAAHDLRAALRNVDALLAGEA
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARHAASIDIAMPETPLSVRADPVEMEQVMFNLLRNALDAPTDVEGRKAITARLSRKGDFA
HHHHCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEE
VFEVADNGPGVAPDLRDRLFTPFVTTKKDGMGLGLALSQRLVERAGGEILYVDSENGALF
EEEEECCCCCCCCHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEE
RVIFPVAAEARNP
EEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]