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The map label for this gene is ksgA

Identifier: 15888443

GI number: 15888443

Start: 1091799

End: 1092629

Strand: Reverse

Name: ksgA

Synonym: Atu1103

Alternate gene names: 15888443

Gene position: 1092629-1091799 (Counterclockwise)

Preceding gene: 159184615

Following gene: 15888442

Centisome position: 38.45

GC content: 63.42

Gene sequence:

>831_bases
ATGGCCGCCATCGATGGACTGCCGCCGCTGCGCGATGTCATCCAGCGCCACGGGCTCGATGCGAAGAAATCGCTTGGTCA
GAATTTCCTGTTCGACCTCAACCTGACCCAGAAGATCGCCCGCACCGCCGGACCGCTCGATGGGGTGACGGTGATCGAGG
TCGGTCCCGGCCCCGGCGGCCTCACCCGTGCCATTCTGTCGCTTGGCGCGAAAAAGGTCATAGCCGTCGAGCGCGACAGC
CGCTGTCTGCCCGTTCTCGCGGAAATCGAAGCGCATTATCCCGGACGACTCGAGGTCATCGAGGGCGACGCGCTGAAGAC
GGATTTCGAGGCGCTGGTTCCCGCTGGCGAGCCTGTGCGCATCATTGCCAATCTGCCCTATAATGTCGGCACGCAATTGC
TGGTCAACTGGCTACTGCCGAGGGAGTGGCCGCCCTTCTGGCTCTCCATGACGCTGATGTTCCAGAAGGAAGTGGGCCAG
CGCATCGTGGCCGAAGAAGGCGACAATCACTACGGCCGCCTCGGCGTTCTCGCCGGCTGGCGCACCGTTTCCGAAATGGC
CTTCGACGTTCCGCCCCAGGCCTTCAGCCCACCGCCGAAGGTGACCTCGACGGTCGTGCATCTGCTGCCCAAGGACAAGC
CGTTGCCCTGCGACGTCGCCAAACTTGAAAGGGTTACGGAGGCGGCCTTCGGCCAGCGCCGCAAAATGCTGCGCCAGAGC
GTCAAGAGCCTTGGTGGAGAAACCCTGCTGGAAAAGGCGGGCATCGATCCCACGCGGCGGGCGGAAACGCTCTCGGTGGA
AGAATTCGTGACGCTGGCGAACTGCCTGTAA

Upstream 100 bases:

>100_bases
CCTTCGGCATTGCCGGCCAGGGCATTGCCAGTGAGACGAGCCTTGTCGCGGCGCTGAAGATGGCAGCGGAGATTTATGCC
CGCAGCCGGGTTGGCGCGTA

Downstream 100 bases:

>100_bases
ATTCTGCACTTGCTCGCTGTTCCGAACGGCGCCAAGGGCTCGTGGATACCTGCGAATAGAGGAACAAGTGCGTGGAGAGT
GCCGGTTTTCACTATAAGCT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MAAIDGLPPLRDVIQRHGLDAKKSLGQNFLFDLNLTQKIARTAGPLDGVTVIEVGPGPGGLTRAILSLGAKKVIAVERDS
RCLPVLAEIEAHYPGRLEVIEGDALKTDFEALVPAGEPVRIIANLPYNVGTQLLVNWLLPREWPPFWLSMTLMFQKEVGQ
RIVAEEGDNHYGRLGVLAGWRTVSEMAFDVPPQAFSPPPKVTSTVVHLLPKDKPLPCDVAKLERVTEAAFGQRRKMLRQS
VKSLGGETLLEKAGIDPTRRAETLSVEEFVTLANCL

Sequences:

>Translated_276_residues
MAAIDGLPPLRDVIQRHGLDAKKSLGQNFLFDLNLTQKIARTAGPLDGVTVIEVGPGPGGLTRAILSLGAKKVIAVERDS
RCLPVLAEIEAHYPGRLEVIEGDALKTDFEALVPAGEPVRIIANLPYNVGTQLLVNWLLPREWPPFWLSMTLMFQKEVGQ
RIVAEEGDNHYGRLGVLAGWRTVSEMAFDVPPQAFSPPPKVTSTVVHLLPKDKPLPCDVAKLERVTEAAFGQRRKMLRQS
VKSLGGETLLEKAGIDPTRRAETLSVEEFVTLANCL
>Mature_275_residues
AAIDGLPPLRDVIQRHGLDAKKSLGQNFLFDLNLTQKIARTAGPLDGVTVIEVGPGPGGLTRAILSLGAKKVIAVERDSR
CLPVLAEIEAHYPGRLEVIEGDALKTDFEALVPAGEPVRIIANLPYNVGTQLLVNWLLPREWPPFWLSMTLMFQKEVGQR
IVAEEGDNHYGRLGVLAGWRTVSEMAFDVPPQAFSPPPKVTSTVVHLLPKDKPLPCDVAKLERVTEAAFGQRRKMLRQSV
KSLGGETLLEKAGIDPTRRAETLSVEEFVTLANCL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=291, Percent_Identity=38.8316151202749, Blast_Score=174, Evalue=5e-44,
Organism=Homo sapiens, GI7657198, Length=240, Percent_Identity=33.75, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1786236, Length=267, Percent_Identity=37.4531835205993, Blast_Score=155, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI25141369, Length=307, Percent_Identity=32.8990228013029, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25146882, Length=229, Percent_Identity=34.061135371179, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=250, Percent_Identity=30.8, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21357273, Length=289, Percent_Identity=35.2941176470588, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21358017, Length=245, Percent_Identity=34.2857142857143, Blast_Score=111, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_AGRT5 (Q8UGD5)

Other databases:

- EMBL:   AE007869
- PIR:   AF2712
- PIR:   D97494
- RefSeq:   NP_354124.1
- ProteinModelPortal:   Q8UGD5
- SMR:   Q8UGD5
- STRING:   Q8UGD5
- GeneID:   1133141
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1103
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- PhylomeDB:   Q8UGD5
- ProtClustDB:   PRK00274
- BioCyc:   ATUM176299-1:ATU1103-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30179; Mature: 30048

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 28-28 BINDING 30-30 BINDING 55-55 BINDING 77-77 BINDING 103-103 BINDING 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIDGLPPLRDVIQRHGLDAKKSLGQNFLFDLNLTQKIARTAGPLDGVTVIEVGPGPGG
CCCCCCCCHHHHHHHHCCCCHHHHCCCCEEEECCHHHHHHHHCCCCCCEEEEEECCCCCH
LTRAILSLGAKKVIAVERDSRCLPVLAEIEAHYPGRLEVIEGDALKTDFEALVPAGEPVR
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCHHCCHHHHCCCCCCEE
IIANLPYNVGTQLLVNWLLPREWPPFWLSMTLMFQKEVGQRIVAEEGDNHYGRLGVLAGW
EEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHH
RTVSEMAFDVPPQAFSPPPKVTSTVVHLLPKDKPLPCDVAKLERVTEAAFGQRRKMLRQS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VKSLGGETLLEKAGIDPTRRAETLSVEEFVTLANCL
HHHCCHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
AAIDGLPPLRDVIQRHGLDAKKSLGQNFLFDLNLTQKIARTAGPLDGVTVIEVGPGPGG
CCCCCCCHHHHHHHHCCCCHHHHCCCCEEEECCHHHHHHHHCCCCCCEEEEEECCCCCH
LTRAILSLGAKKVIAVERDSRCLPVLAEIEAHYPGRLEVIEGDALKTDFEALVPAGEPVR
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCHHCCHHHHCCCCCCEE
IIANLPYNVGTQLLVNWLLPREWPPFWLSMTLMFQKEVGQRIVAEEGDNHYGRLGVLAGW
EEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHH
RTVSEMAFDVPPQAFSPPPKVTSTVVHLLPKDKPLPCDVAKLERVTEAAFGQRRKMLRQS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VKSLGGETLLEKAGIDPTRRAETLSVEEFVTLANCL
HHHCCHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194