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The map label for this gene is ada [H]

Identifier: 15888240

GI number: 15888240

Start: 892046

End: 893182

Strand: Direct

Name: ada [H]

Synonym: Atu0901

Alternate gene names: 15888240

Gene position: 892046-893182 (Clockwise)

Preceding gene: 15888239

Following gene: 159184525

Centisome position: 31.39

GC content: 65.0

Gene sequence:

>1137_bases
ATGCCCGTTTTTCGGCGGCATGCCTCTCAAAATTGCGCTAGCATCCCTCCCATGATGCTGTTCAAACGCCCCGACACTGA
AACACTCTATTTCGCCCTTGTGAACAAGGATCCCGCCTATGAAGGCGTGGCCTATGTCTGCGTGACATCCACGAAGATTT
TCTGCCGCTTCACCTGCACGGCGCGCAAGCCGAAGATCGAGAACTGCCGGTTCCGGGAGACCATCACCGAATGCCTGGAG
GCGGGTTTTCGCCCTTGCAAGCGTTGCAAGCCGATGTTGTCTTACGGCACGCCCGACGGGACCGTTACTGCGCTCCTGAC
AGCGCTGGAAAACGAACCGGCGCGGCGCTGGCGCGAGGAGGATGTGGTGGCGCTCGGCCATGATCCCTCCACCGTGCGGC
GCAGCTTCAAGCGCCGTTTCGGCATGAGCTTTCTGGAAATCGCCCGGCTGCGGCGGGTGGGGGACGCGGCGGAAAGCCTT
GCCTCCGGCGAGGCGGTGATCGGCGCGCAGATCGATGCCGGTTATGAATCCGGCAGCGGCTTTCGCTCGGCCATCAACCA
GTTGTTCGGCACCTCTCCCGCCGCCATGAAGGGCAGGGGGTTCCTGAAGGGCGACTGGATCGACACGCCGATTGGCCCGA
TGCTGGCCGTTGCCGACGACCACGCCCTGCATCTCCTCGAATTCGCCGACCGGCCGGCTCTTCCCGCCGAGCTGAAACGG
CTGAAGGCCCGCACCGGCGCGGATATGGTGCTGGGCCGCACCGCGGTCACCGGCCAGATCGCCGCGGAACTTGCGGCCTA
TTTCGCCGGCCGTTCGGGCAGTTTCGAAACCCGGCTCGCCGGCCACGGCACGCCCTTCGAGCGCGATGTCTGGCGGAACC
TGCGGGAAATCCCGGCGGGTGAGATCGTCAGCTATTCCTCGCTCGCAACCGCGATGGAGAGGCCTACCGCCGTGCGGGCG
GTTGCCCGCGCCAATGGCGCCAACCAGCTTGCGATTGTCGTTCCCTGCCATCGGGTGCTCGGTGCGGATGGCAGTCTGAC
GGGGTATGGTGGCGGGCTGTGGCGCAAGAAATGGCTGATTGAGCATGAGCGCCGCATGGCGCGGGGGCAGATGCTGGGAA
TGCCGCTCGCTTCCTGA

Upstream 100 bases:

>100_bases
CGACGCGCGTCTGCGCGACGGAGAGAGTCCTTTCAACCCAAGGACTTGGGCTTGCTGGATTCCTGTGACAAGCACAGGAA
TGAGGTCGTGAGCCACATCC

Downstream 100 bases:

>100_bases
GCGGCCTGGCCGTTGGGTTTGAGTAGGTCGTTCACTTAGTTGAGCGGCGTGGGTGGGGCTTACCCCCCCTCTGCCCTGCC
GGGCATCTCCCCCTCAAGGG

Product: 6-O-methylguanine-DNA methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MPVFRRHASQNCASIPPMMLFKRPDTETLYFALVNKDPAYEGVAYVCVTSTKIFCRFTCTARKPKIENCRFRETITECLE
AGFRPCKRCKPMLSYGTPDGTVTALLTALENEPARRWREEDVVALGHDPSTVRRSFKRRFGMSFLEIARLRRVGDAAESL
ASGEAVIGAQIDAGYESGSGFRSAINQLFGTSPAAMKGRGFLKGDWIDTPIGPMLAVADDHALHLLEFADRPALPAELKR
LKARTGADMVLGRTAVTGQIAAELAAYFAGRSGSFETRLAGHGTPFERDVWRNLREIPAGEIVSYSSLATAMERPTAVRA
VARANGANQLAIVVPCHRVLGADGSLTGYGGGLWRKKWLIEHERRMARGQMLGMPLAS

Sequences:

>Translated_378_residues
MPVFRRHASQNCASIPPMMLFKRPDTETLYFALVNKDPAYEGVAYVCVTSTKIFCRFTCTARKPKIENCRFRETITECLE
AGFRPCKRCKPMLSYGTPDGTVTALLTALENEPARRWREEDVVALGHDPSTVRRSFKRRFGMSFLEIARLRRVGDAAESL
ASGEAVIGAQIDAGYESGSGFRSAINQLFGTSPAAMKGRGFLKGDWIDTPIGPMLAVADDHALHLLEFADRPALPAELKR
LKARTGADMVLGRTAVTGQIAAELAAYFAGRSGSFETRLAGHGTPFERDVWRNLREIPAGEIVSYSSLATAMERPTAVRA
VARANGANQLAIVVPCHRVLGADGSLTGYGGGLWRKKWLIEHERRMARGQMLGMPLAS
>Mature_377_residues
PVFRRHASQNCASIPPMMLFKRPDTETLYFALVNKDPAYEGVAYVCVTSTKIFCRFTCTARKPKIENCRFRETITECLEA
GFRPCKRCKPMLSYGTPDGTVTALLTALENEPARRWREEDVVALGHDPSTVRRSFKRRFGMSFLEIARLRRVGDAAESLA
SGEAVIGAQIDAGYESGSGFRSAINQLFGTSPAAMKGRGFLKGDWIDTPIGPMLAVADDHALHLLEFADRPALPAELKRL
KARTGADMVLGRTAVTGQIAAELAAYFAGRSGSFETRLAGHGTPFERDVWRNLREIPAGEIVSYSSLATAMERPTAVRAV
ARANGANQLAIVVPCHRVLGADGSLTGYGGGLWRKKWLIEHERRMARGQMLGMPLAS

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=176, Percent_Identity=31.25, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788542, Length=347, Percent_Identity=33.4293948126801, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787596, Length=159, Percent_Identity=40.8805031446541, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=46.25, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=46.25, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=82, Percent_Identity=42.6829268292683, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 41427; Mature: 41296

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVFRRHASQNCASIPPMMLFKRPDTETLYFALVNKDPAYEGVAYVCVTSTKIFCRFTCT
CCCHHHHHCCCCCCCCCHHEEECCCCCEEEEEEECCCCCCCCEEEEEEECCEEEEEEEEC
ARKPKIENCRFRETITECLEAGFRPCKRCKPMLSYGTPDGTVTALLTALENEPARRWREE
CCCCCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHCCCCCHHHHHHHHHHCCCHHHHHCCC
DVVALGHDPSTVRRSFKRRFGMSFLEIARLRRVGDAAESLASGEAVIGAQIDAGYESGSG
CEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
FRSAINQLFGTSPAAMKGRGFLKGDWIDTPIGPMLAVADDHALHLLEFADRPALPAELKR
HHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHH
LKARTGADMVLGRTAVTGQIAAELAAYFAGRSGSFETRLAGHGTPFERDVWRNLREIPAG
HHHHCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCC
EIVSYSSLATAMERPTAVRAVARANGANQLAIVVPCHRVLGADGSLTGYGGGLWRKKWLI
HHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECHHHHCCCCCCCCCCCCHHHHHHHH
EHERRMARGQMLGMPLAS
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PVFRRHASQNCASIPPMMLFKRPDTETLYFALVNKDPAYEGVAYVCVTSTKIFCRFTCT
CCHHHHHCCCCCCCCCHHEEECCCCCEEEEEEECCCCCCCCEEEEEEECCEEEEEEEEC
ARKPKIENCRFRETITECLEAGFRPCKRCKPMLSYGTPDGTVTALLTALENEPARRWREE
CCCCCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHCCCCCHHHHHHHHHHCCCHHHHHCCC
DVVALGHDPSTVRRSFKRRFGMSFLEIARLRRVGDAAESLASGEAVIGAQIDAGYESGSG
CEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
FRSAINQLFGTSPAAMKGRGFLKGDWIDTPIGPMLAVADDHALHLLEFADRPALPAELKR
HHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHH
LKARTGADMVLGRTAVTGQIAAELAAYFAGRSGSFETRLAGHGTPFERDVWRNLREIPAG
HHHHCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCC
EIVSYSSLATAMERPTAVRAVARANGANQLAIVVPCHRVLGADGSLTGYGGGLWRKKWLI
HHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECHHHHCCCCCCCCCCCCHHHHHHHH
EHERRMARGQMLGMPLAS
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]