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The map label for this gene is mscL

Identifier: 15887877

GI number: 15887877

Start: 521539

End: 521967

Strand: Direct

Name: mscL

Synonym: Atu0528

Alternate gene names: 15887877

Gene position: 521539-521967 (Clockwise)

Preceding gene: 159184354

Following gene: 159184355

Centisome position: 18.35

GC content: 54.08

Gene sequence:

>429_bases
ATGCTTAACGAATTTAAAACCTTCATTGCCCGCGGCAATGTCATGGACCTGGCCGTGGGTGTTATTATCGGTGCGGCTTT
CAGCAAGATCGTCGATTCCGTTGTCAACGATCTCATCATGCCGATCGTTGGCGCAATCTTCGGCGGGTTCGACTTCTCGA
ACTACTTCCTGCCGCTGAGCTCCAACGTCAACGCCACCTCGCTCGCTGCCGCCCGCGAACAGGGTGCCGTCTTCGCCTAT
GGTAACTTCCTGACGGTTCTTATTAACTTCCTCATCCTGGCCTGGATCATTTTCCTGATGGTCAAGGGTGTCAACAAGCT
CCGCGATTCGGTTGACCGCAAGAAGATTGAAGAAAAGCCGGACGCAGCTCCGCCGCCGGAAGACGTCAAGCTCCTCACCG
AAATCCGCGATCTGCTGAAAACTCGATAA

Upstream 100 bases:

>100_bases
TCTTGACGAATTCCAAATGTGTAAGGGAATCTGTATGGTTGAAGACGTAGGGACAGTCACATAGCTTCAGCTCGCGAAGC
AGGAGAGAGGGACAATCTGA

Downstream 100 bases:

>100_bases
GACCCGGATCAGTCCCCGGCCTGATCTGCCTGCCTGTGCGGTCGGCGGGTTCCTGCCGGGGATGATCCATAAAGAAAATC
CATGATCGCCTCACGGAATA

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MLNEFKTFIARGNVMDLAVGVIIGAAFSKIVDSVVNDLIMPIVGAIFGGFDFSNYFLPLSSNVNATSLAAAREQGAVFAY
GNFLTVLINFLILAWIIFLMVKGVNKLRDSVDRKKIEEKPDAAPPPEDVKLLTEIRDLLKTR

Sequences:

>Translated_142_residues
MLNEFKTFIARGNVMDLAVGVIIGAAFSKIVDSVVNDLIMPIVGAIFGGFDFSNYFLPLSSNVNATSLAAAREQGAVFAY
GNFLTVLINFLILAWIIFLMVKGVNKLRDSVDRKKIEEKPDAAPPPEDVKLLTEIRDLLKTR
>Mature_142_residues
MLNEFKTFIARGNVMDLAVGVIIGAAFSKIVDSVVNDLIMPIVGAIFGGFDFSNYFLPLSSNVNATSLAAAREQGAVFAY
GNFLTVLINFLILAWIIFLMVKGVNKLRDSVDRKKIEEKPDAAPPPEDVKLLTEIRDLLKTR

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=144, Percent_Identity=48.6111111111111, Blast_Score=110, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_AGRT5 (Q8UHY0)

Other databases:

- EMBL:   AE007869
- PIR:   AD2641
- PIR:   F97423
- RefSeq:   NP_353558.1
- ProteinModelPortal:   Q8UHY0
- SMR:   Q8UHY0
- STRING:   Q8UHY0
- GeneID:   1132566
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0528
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- PhylomeDB:   Q8UHY0
- BioCyc:   ATUM176299-1:ATU0528-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15607; Mature: 15607

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xbf8c92c)-; HASH(0xd3847bc)-; HASH(0xc9dd5c0)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNEFKTFIARGNVMDLAVGVIIGAAFSKIVDSVVNDLIMPIVGAIFGGFDFSNYFLPLS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
SNVNATSLAAAREQGAVFAYGNFLTVLINFLILAWIIFLMVKGVNKLRDSVDRKKIEEKP
CCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DAAPPPEDVKLLTEIRDLLKTR
CCCCCHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MLNEFKTFIARGNVMDLAVGVIIGAAFSKIVDSVVNDLIMPIVGAIFGGFDFSNYFLPLS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
SNVNATSLAAAREQGAVFAYGNFLTVLINFLILAWIIFLMVKGVNKLRDSVDRKKIEEKP
CCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DAAPPPEDVKLLTEIRDLLKTR
CCCCCHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194