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The map label for this gene is cheB

Identifier: 15887867

GI number: 15887867

Start: 509606

End: 510661

Strand: Direct

Name: cheB

Synonym: Atu0519

Alternate gene names: 15887867

Gene position: 509606-510661 (Clockwise)

Preceding gene: 15887866

Following gene: 15887868

Centisome position: 17.93

GC content: 64.3

Gene sequence:

>1056_bases
ATGAGCGCACTCGCACGGGTCCTCGTCGTTGACGACTCCCCCACCATGCGCGGCCTGATTTCGGCCGTCCTCAAGGCCGA
TCCGGAAGTCGAGGTGGTCGGCCAGGCCGGCAATGCCATGGAAGCACGTGCCGCCATCAAGCAGCTCAATCCCGATGTTG
TGACGCTCGACATCGAGATGCCCGAGATGAATGGCCTCGAATTTCTCGAAAAGATCATGCGCCTGCGCCCCATGCCGGTC
ATCATGGTCTCGTCACTGACCCATCGCGGCGCGGATGCATCGCTTGCCGCGCTTGAAATCGGCGCTTTCGACTGTGTCGG
CAAGCCGGCTCCCGGTGACGCGCGCCCCTTCGGCGATCTGGCCGACAAGGTGAAGGCCGCCGCCCGTTCGCAACATGCCG
CCTACCGCGCCACCCGGCCGGAAACCGCTGCCGCACCGCAGCCGGTTCCGATGAGCGAATATCGGGCGGGTCGTAAGGTC
GTCGCCATCGGCTCCTCCACCGGCGGCGTCGAAGCGCTAATCGCGGTGCTGCAGAAATTCCCGGCCAATTGCCCGCCGAC
AGTCATTACCCAGCACATGCCGCCGACCTTCACCAAGAGTTTCGCGGAGAGGCTGAACCGCATCTGCGCCCCCGTGGTGG
AAGAGGCGACGGACGGCGCGCGCCTGCAGACCGGCAAGATCTACCTCGCGCCGGGCGGTGAGCGTCATTTACAGATCGCC
AACCGGTCCGCACCCTGCTGCCGGCTTCTGGACCGCGACCCCGTCAATGGCCATCGCCCCTCCGTCGATGTGCTGTTCGA
TTCGGTGGCCGAGCTTGCCGGACGCAACGCCGTCGGTGTCATCTTGACCGGCATGGGCCGCGATGGCGCGGCCGGGCTTT
TGAAAATGCGCCATGCCGGTGCGCGCACCGTTGGCCAGAACGAAAAAACATGTGTCGTCTATGGCATGCCCCGCGTGGCC
TATGAGCTTGGCGCGGTGGAACAGCAATTGCCGTTGGCCTCCATCGGCGAAGAAATCCTGAAACTAACCACTGCCCGCAA
AGAAGGTGCTGACTAA

Upstream 100 bases:

>100_bases
TCGGCCATTCCGAACGTGTGTCCGGTGACGCCAAGAACGTGTTCGACAATACCGGCATCACCACCTACCGCTTCATCGGT
CACGCATCCGGGAGGAAGGC

Downstream 100 bases:

>100_bases
TGTCTCTCGCAGAAAAGATCAAAGTTCTGATCGTTGACGATCAGGTGACCAGCCGGCTGCTCCTCAGCGATGCGCTGACA
CAGCTGGGCTTCAAGCAGAT

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MSALARVLVVDDSPTMRGLISAVLKADPEVEVVGQAGNAMEARAAIKQLNPDVVTLDIEMPEMNGLEFLEKIMRLRPMPV
IMVSSLTHRGADASLAALEIGAFDCVGKPAPGDARPFGDLADKVKAAARSQHAAYRATRPETAAAPQPVPMSEYRAGRKV
VAIGSSTGGVEALIAVLQKFPANCPPTVITQHMPPTFTKSFAERLNRICAPVVEEATDGARLQTGKIYLAPGGERHLQIA
NRSAPCCRLLDRDPVNGHRPSVDVLFDSVAELAGRNAVGVILTGMGRDGAAGLLKMRHAGARTVGQNEKTCVVYGMPRVA
YELGAVEQQLPLASIGEEILKLTTARKEGAD

Sequences:

>Translated_351_residues
MSALARVLVVDDSPTMRGLISAVLKADPEVEVVGQAGNAMEARAAIKQLNPDVVTLDIEMPEMNGLEFLEKIMRLRPMPV
IMVSSLTHRGADASLAALEIGAFDCVGKPAPGDARPFGDLADKVKAAARSQHAAYRATRPETAAAPQPVPMSEYRAGRKV
VAIGSSTGGVEALIAVLQKFPANCPPTVITQHMPPTFTKSFAERLNRICAPVVEEATDGARLQTGKIYLAPGGERHLQIA
NRSAPCCRLLDRDPVNGHRPSVDVLFDSVAELAGRNAVGVILTGMGRDGAAGLLKMRHAGARTVGQNEKTCVVYGMPRVA
YELGAVEQQLPLASIGEEILKLTTARKEGAD
>Mature_350_residues
SALARVLVVDDSPTMRGLISAVLKADPEVEVVGQAGNAMEARAAIKQLNPDVVTLDIEMPEMNGLEFLEKIMRLRPMPVI
MVSSLTHRGADASLAALEIGAFDCVGKPAPGDARPFGDLADKVKAAARSQHAAYRATRPETAAAPQPVPMSEYRAGRKVV
AIGSSTGGVEALIAVLQKFPANCPPTVITQHMPPTFTKSFAERLNRICAPVVEEATDGARLQTGKIYLAPGGERHLQIAN
RSAPCCRLLDRDPVNGHRPSVDVLFDSVAELAGRNAVGVILTGMGRDGAAGLLKMRHAGARTVGQNEKTCVVYGMPRVAY
ELGAVEQQLPLASIGEEILKLTTARKEGAD

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=345, Percent_Identity=49.8550724637681, Blast_Score=321, Evalue=4e-89,
Organism=Escherichia coli, GI87082052, Length=107, Percent_Identity=34.5794392523364, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_AGRT5 (O85128)

Other databases:

- EMBL:   AF044495
- EMBL:   AE007869
- PIR:   AC2640
- PIR:   D97422
- RefSeq:   NP_353548.1
- ProteinModelPortal:   O85128
- SMR:   O85128
- STRING:   O85128
- GeneID:   1132557
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0519
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   PPSVADF
- PhylomeDB:   O85128
- ProtClustDB:   PRK00742
- BioCyc:   ATUM176299-1:ATU0519-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 37196; Mature: 37065

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 166-166 ACT_SITE 192-192 ACT_SITE 288-288

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALARVLVVDDSPTMRGLISAVLKADPEVEVVGQAGNAMEARAAIKQLNPDVVTLDIEM
CCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEEC
PEMNGLEFLEKIMRLRPMPVIMVSSLTHRGADASLAALEIGAFDCVGKPAPGDARPFGDL
CCCCCHHHHHHHHHHCCCCEEEEHHHHCCCCCCCEEEEEECCHHCCCCCCCCCCCCHHHH
ADKVKAAARSQHAAYRATRPETAAAPQPVPMSEYRAGRKVVAIGSSTGGVEALIAVLQKF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHC
PANCPPTVITQHMPPTFTKSFAERLNRICAPVVEEATDGARLQTGKIYLAPGGERHLQIA
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEECCCCCCEEEEC
NRSAPCCRLLDRDPVNGHRPSVDVLFDSVAELAGRNAVGVILTGMGRDGAAGLLKMRHAG
CCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCC
ARTVGQNEKTCVVYGMPRVAYELGAVEQQLPLASIGEEILKLTTARKEGAD
CCCCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SALARVLVVDDSPTMRGLISAVLKADPEVEVVGQAGNAMEARAAIKQLNPDVVTLDIEM
CCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEEC
PEMNGLEFLEKIMRLRPMPVIMVSSLTHRGADASLAALEIGAFDCVGKPAPGDARPFGDL
CCCCCHHHHHHHHHHCCCCEEEEHHHHCCCCCCCEEEEEECCHHCCCCCCCCCCCCHHHH
ADKVKAAARSQHAAYRATRPETAAAPQPVPMSEYRAGRKVVAIGSSTGGVEALIAVLQKF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHC
PANCPPTVITQHMPPTFTKSFAERLNRICAPVVEEATDGARLQTGKIYLAPGGERHLQIA
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEECCCCCCEEEEC
NRSAPCCRLLDRDPVNGHRPSVDVLFDSVAELAGRNAVGVILTGMGRDGAAGLLKMRHAG
CCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCC
ARTVGQNEKTCVVYGMPRVAYELGAVEQQLPLASIGEEILKLTTARKEGAD
CCCCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194