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The map label for this gene is trmB

Identifier: 15887714

GI number: 15887714

Start: 357787

End: 358485

Strand: Direct

Name: trmB

Synonym: Atu0363

Alternate gene names: 15887714

Gene position: 357787-358485 (Clockwise)

Preceding gene: 15887713

Following gene: 15887717

Centisome position: 12.59

GC content: 58.94

Gene sequence:

>699_bases
ATGACGGAAGAACGGCGTTCGCGCGCAACGGAAGCGTTTTTTGGCAGACGCAAGGGCAAGCCCCTGCGCAATCAGCAGGT
CGATACGATCGAGAACCTGCTGCCTCTGCTGAAAATCGATCTGGAAAGCGTGCCCCCGCAAAATCTGGTCGCTCTCTTTC
CGGCGGATGTACGGTCGATCCGCCTGGAAATCGGTTTCGGCGGTGGCGAGCATCTCGCCCACCGCGCGGTCGAAAACCCC
GAGACGGGCTTCATCGGCGTCGAGCCTTTCGTGAATTCCATGGCCAAACTGCTCGCGACGGTCCGCGAACGCGAACTGAT
GAATATCCGCCTTTACGACGATGATGCGACGCAGCTGCTCGACTGGTTGCCCGAGGGGTCGATCGATCACATCGATCTGC
TTTATCCCGACCCGTGGCCGAAGAAGAAGCACTGGAAGCGCCGCTTCGTTTCCGACGTCAATCTCGCCCGTTTCCATCGT
GTGTTGAAACCCGGCGGCAAGTTCTGCTTTGCGTCGGATATAGATACTTACGTCAACTGGACGCTGCAGCATTGTGCCCG
CCATGGCGGTTTTGAGTGGACGGCAACAAGCGCTGACGATTGGCGCACGCCCTATGCCAACTGGCCAGGCACGCGCTACG
AGAACAAGGCGAAACGAGAAGGCCGCAGCTCGGCCTATCTCACCTTCATCCGTCGCTAA

Upstream 100 bases:

>100_bases
TTGGCGGTGATTGAGAGATACCGTCTACAACTTTTGTAACCCGCACAGTGCCCCATGTGGCGCTGTGCGGGTTAAGTCTA
TTTGTTGTTGAGAGTATGAG

Downstream 100 bases:

>100_bases
AGCATTTCCGGTAAAACTGCGTAGCGGTTTTACGCCCGGAAAATGCGCCGGCAGCCTGATCCCTCAGAGCGCCGCATCCA
CTTCCGCTGCCTGCGGAATG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MTEERRSRATEAFFGRRKGKPLRNQQVDTIENLLPLLKIDLESVPPQNLVALFPADVRSIRLEIGFGGGEHLAHRAVENP
ETGFIGVEPFVNSMAKLLATVRERELMNIRLYDDDATQLLDWLPEGSIDHIDLLYPDPWPKKKHWKRRFVSDVNLARFHR
VLKPGGKFCFASDIDTYVNWTLQHCARHGGFEWTATSADDWRTPYANWPGTRYENKAKREGRSSAYLTFIRR

Sequences:

>Translated_232_residues
MTEERRSRATEAFFGRRKGKPLRNQQVDTIENLLPLLKIDLESVPPQNLVALFPADVRSIRLEIGFGGGEHLAHRAVENP
ETGFIGVEPFVNSMAKLLATVRERELMNIRLYDDDATQLLDWLPEGSIDHIDLLYPDPWPKKKHWKRRFVSDVNLARFHR
VLKPGGKFCFASDIDTYVNWTLQHCARHGGFEWTATSADDWRTPYANWPGTRYENKAKREGRSSAYLTFIRR
>Mature_231_residues
TEERRSRATEAFFGRRKGKPLRNQQVDTIENLLPLLKIDLESVPPQNLVALFPADVRSIRLEIGFGGGEHLAHRAVENPE
TGFIGVEPFVNSMAKLLATVRERELMNIRLYDDDATQLLDWLPEGSIDHIDLLYPDPWPKKKHWKRRFVSDVNLARFHRV
LKPGGKFCFASDIDTYVNWTLQHCARHGGFEWTATSADDWRTPYANWPGTRYENKAKREGRSSAYLTFIRR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=219, Percent_Identity=31.5068493150685, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_AGRT5 (Q8UID4)

Other databases:

- EMBL:   AE007869
- PIR:   AC2621
- PIR:   C97403
- RefSeq:   NP_353395.1
- ProteinModelPortal:   Q8UID4
- SMR:   Q8UID4
- STRING:   Q8UID4
- GeneID:   1132401
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0363
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   RYGRKAE
- PhylomeDB:   Q8UID4
- ProtClustDB:   PRK00121
- BioCyc:   ATUM176299-1:ATU0363-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26864; Mature: 26733

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: BINDING 63-63 BINDING 88-88 BINDING 115-115 BINDING 137-137 BINDING 141-141 BINDING 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEERRSRATEAFFGRRKGKPLRNQQVDTIENLLPLLKIDLESVPPQNLVALFPADVRSI
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEE
RLEIGFGGGEHLAHRAVENPETGFIGVEPFVNSMAKLLATVRERELMNIRLYDDDATQLL
EEEEECCCHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHH
DWLPEGSIDHIDLLYPDPWPKKKHWKRRFVSDVNLARFHRVLKPGGKFCFASDIDTYVNW
HHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCHHHHHHH
TLQHCARHGGFEWTATSADDWRTPYANWPGTRYENKAKREGRSSAYLTFIRR
HHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEECC
>Mature Secondary Structure 
TEERRSRATEAFFGRRKGKPLRNQQVDTIENLLPLLKIDLESVPPQNLVALFPADVRSI
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEE
RLEIGFGGGEHLAHRAVENPETGFIGVEPFVNSMAKLLATVRERELMNIRLYDDDATQLL
EEEEECCCHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHH
DWLPEGSIDHIDLLYPDPWPKKKHWKRRFVSDVNLARFHRVLKPGGKFCFASDIDTYVNW
HHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCHHHHHHH
TLQHCARHGGFEWTATSADDWRTPYANWPGTRYENKAKREGRSSAYLTFIRR
HHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194