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The map label for this gene is prfC [H]

Identifier: 15887665

GI number: 15887665

Start: 307457

End: 309040

Strand: Direct

Name: prfC [H]

Synonym: Atu0315

Alternate gene names: 15887665

Gene position: 307457-309040 (Clockwise)

Preceding gene: 159184251

Following gene: 159184253

Centisome position: 10.82

GC content: 60.92

Gene sequence:

>1584_bases
ATGGCCGAAACACTTGCCGAGGCGGTCTCCCGCCGCCGCACCTTTGCTATTATCGCGCACCCGGACGCGGGTAAGACCAC
GCTTACCGAAAAACTGCTGCTGTTCGGTGGAGCGATCCAGCTTGCCGGTGAGGTGAAGGCGAAGAAGGACCGCATCCAGA
CCCGTTCGGACTGGATGAAGATCGAGCGCGAGCGCGGCATTTCGGTCGTCACCTCGGTCATGACCTTCGAATATAACGAC
CGCGTCTTCAACATTCTCGATACGCCCGGCCACGAAGACTTCGCTGACGATACTTACCGCACGCTGACGGCGGTGGATGC
TGCGATCATGGTCATCGACGCCGCCAAGGGTATCGAGCCGCGCACGCTGAAGCTCTTCGAAGTCTGCCGCATGCGCGATA
TTCCGATCATCACCTTCATCAACAAGATGGACCGCGAAAGCCGCGATCCCTTCGAGATTCTGGATGAGGTCGAGGAGAAG
CTGGCGCTCGATACCGCGCCGATTACCTGGCCGGTCGGCCGGGCGAAAAGCTTCTGTGGCGCTTACAATCTTGCCGAAAA
CACCTTTCGTGGCAACGACACGCAGGTGGACGGCCTTTCGGTCAACGGCCCGCAGGCGGTCGCCGACCGTCTGCCGGAAA
ACGAACGCAAGGTCTTTGTCGAAGAGACGGAACTGGCGCTGGAAGCCTGCCGTCCTTTCGACCGTCAGGCGTTTCTCGAA
GGTCATATGACGCCGGTGTTCTTCGGTTCGGCACTGCGCAATTTCGGTGTGCGCGACCTCATCAATGCGCTGGGTGATTT
CGCCCCGCCGCCGCGCGATCAGGTAGCGGATACCCGCGTCGTGCATGCGGCAGAAGACAAGATGACGGCTTTCGTCTTCA
AAATTCAGGCCAATATGGACCCCAACCACCGCGACCGTATTGCCTTTGCCCGCATCTGCTCCGGCAAGCTGGAGCGCGGC
ATGAAGGCGCGGTTGTCGCGTACCGGCAAGCTGATGGGGCTCACCGCGCCGCAATTCTTCTTCGCCTCGCAGCGTCAGTT
GGCCGATACCGCCTTTGCGGGCGATGTGGTCGGCATTCCCAACCACGGCACGCTGCGCATCGGCGATACGCTGACCGAGG
GCGAAAACCTTGTCTTCCAGGGTGTGCCGAACTTCTCGCCGGAAATCCTGCGCCGCGTGCGGCTGGAGGACGCCATGAAG
GCGAAGAAGCTGAAGGAAGCGCTGCAGCAGATGGCGGAAGAGGGCGTCGTACAGCTCTTCTCGCCGGAAGACGGTTCGCC
CGCCATCGTCGGCGTCGTCGGCGCACTGCAGCTCGACGTGTTGAAGGAACGGCTGATGGGCGAATATGGCCTGCCGGTCT
CGTTCGAAATGTCGCGCTTTTCGGTCTGCCGCTGGATTTCGTCGGATCAGCCGGCGGAGATGGACAAGTTCCTCAACGTC
AAGCGTGGTGATATCGCCCGCGATCTCGATGGCGATCCGGTGTTTCTGGCGCAGGACGCCTTCTCGCTGCGGTATGAATC
CGAGCGGTTTCCGGCAATCAAGATGGTCGCCATCAAGGAATATCACGTCGCCAAGGCGGCGTGA

Upstream 100 bases:

>100_bases
TGAACGGTCATGCGGCATTAGTTTGCCGAGATCGCGGCGAGGTCAATTGCAAATGCGGGCCTGAAACGCTAGATAGCCCG
CAATTCACAGGATTTGTCAG

Downstream 100 bases:

>100_bases
TATCAGCCGGATTGCACTTTGAATGCCAAGGGCTTAGTTTCCCCGTAAACGGGAAACTGGTCCGGCATCATGACGCTTTC
AGGCAAACATATTCTCCTCA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MAETLAEAVSRRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMKIERERGISVVTSVMTFEYND
RVFNILDTPGHEDFADDTYRTLTAVDAAIMVIDAAKGIEPRTLKLFEVCRMRDIPIITFINKMDRESRDPFEILDEVEEK
LALDTAPITWPVGRAKSFCGAYNLAENTFRGNDTQVDGLSVNGPQAVADRLPENERKVFVEETELALEACRPFDRQAFLE
GHMTPVFFGSALRNFGVRDLINALGDFAPPPRDQVADTRVVHAAEDKMTAFVFKIQANMDPNHRDRIAFARICSGKLERG
MKARLSRTGKLMGLTAPQFFFASQRQLADTAFAGDVVGIPNHGTLRIGDTLTEGENLVFQGVPNFSPEILRRVRLEDAMK
AKKLKEALQQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLMGEYGLPVSFEMSRFSVCRWISSDQPAEMDKFLNV
KRGDIARDLDGDPVFLAQDAFSLRYESERFPAIKMVAIKEYHVAKAA

Sequences:

>Translated_527_residues
MAETLAEAVSRRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMKIERERGISVVTSVMTFEYND
RVFNILDTPGHEDFADDTYRTLTAVDAAIMVIDAAKGIEPRTLKLFEVCRMRDIPIITFINKMDRESRDPFEILDEVEEK
LALDTAPITWPVGRAKSFCGAYNLAENTFRGNDTQVDGLSVNGPQAVADRLPENERKVFVEETELALEACRPFDRQAFLE
GHMTPVFFGSALRNFGVRDLINALGDFAPPPRDQVADTRVVHAAEDKMTAFVFKIQANMDPNHRDRIAFARICSGKLERG
MKARLSRTGKLMGLTAPQFFFASQRQLADTAFAGDVVGIPNHGTLRIGDTLTEGENLVFQGVPNFSPEILRRVRLEDAMK
AKKLKEALQQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLMGEYGLPVSFEMSRFSVCRWISSDQPAEMDKFLNV
KRGDIARDLDGDPVFLAQDAFSLRYESERFPAIKMVAIKEYHVAKAA
>Mature_526_residues
AETLAEAVSRRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMKIERERGISVVTSVMTFEYNDR
VFNILDTPGHEDFADDTYRTLTAVDAAIMVIDAAKGIEPRTLKLFEVCRMRDIPIITFINKMDRESRDPFEILDEVEEKL
ALDTAPITWPVGRAKSFCGAYNLAENTFRGNDTQVDGLSVNGPQAVADRLPENERKVFVEETELALEACRPFDRQAFLEG
HMTPVFFGSALRNFGVRDLINALGDFAPPPRDQVADTRVVHAAEDKMTAFVFKIQANMDPNHRDRIAFARICSGKLERGM
KARLSRTGKLMGLTAPQFFFASQRQLADTAFAGDVVGIPNHGTLRIGDTLTEGENLVFQGVPNFSPEILRRVRLEDAMKA
KKLKEALQQMAEEGVVQLFSPEDGSPAIVGVVGALQLDVLKERLMGEYGLPVSFEMSRFSVCRWISSDQPAEMDKFLNVK
RGDIARDLDGDPVFLAQDAFSLRYESERFPAIKMVAIKEYHVAKAA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=473, Percent_Identity=26.215644820296, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI19923640, Length=505, Percent_Identity=26.5346534653465, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI25306283, Length=398, Percent_Identity=28.391959798995, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI25306287, Length=474, Percent_Identity=25.7383966244726, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI157426893, Length=155, Percent_Identity=34.1935483870968, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=30.6122448979592, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=30.8724832214765, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790835, Length=528, Percent_Identity=48.4848484848485, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1789738, Length=488, Percent_Identity=27.6639344262295, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=33.7579617834395, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=34.8993288590604, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=490, Percent_Identity=25.7142857142857, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17556745, Length=473, Percent_Identity=24.5243128964059, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=34.0909090909091, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323098, Length=485, Percent_Identity=25.5670103092783, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322359, Length=547, Percent_Identity=25.9597806215722, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=29.9363057324841, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=29.1139240506329, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=29.1139240506329, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582462, Length=480, Percent_Identity=26.6666666666667, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI221458488, Length=501, Percent_Identity=26.1477045908184, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=31.25, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=32.0261437908497, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=32.0261437908497, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=32.0261437908497, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24656849, Length=154, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574573, Length=144, Percent_Identity=31.25, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58818; Mature: 58687

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETLAEAVSRRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMK
CHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHHHH
IERERGISVVTSVMTFEYNDRVFNILDTPGHEDFADDTYRTLTAVDAAIMVIDAAKGIEP
HHHHCCHHHHHHHHHEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCC
RTLKLFEVCRMRDIPIITFINKMDRESRDPFEILDEVEEKLALDTAPITWPVGRAKSFCG
HHHHHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
AYNLAENTFRGNDTQVDGLSVNGPQAVADRLPENERKVFVEETELALEACRPFDRQAFLE
HHHHHHCCCCCCCCEECCEECCCHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCHHHHHH
GHMTPVFFGSALRNFGVRDLINALGDFAPPPRDQVADTRVVHAAEDKMTAFVFKIQANMD
CCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHCCCCEEEECCCCEEEEEEEEEECCC
PNHRDRIAFARICSGKLERGMKARLSRTGKLMGLTAPQFFFASQRQLADTAFAGDVVGIP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHCCCEEECC
NHGTLRIGDTLTEGENLVFQGVPNFSPEILRRVRLEDAMKAKKLKEALQQMAEEGVVQLF
CCCEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SPEDGSPAIVGVVGALQLDVLKERLMGEYGLPVSFEMSRFSVCRWISSDQPAEMDKFLNV
CCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHCC
KRGDIARDLDGDPVFLAQDAFSLRYESERFPAIKMVAIKEYHVAKAA
CCCCCCCCCCCCEEEEECCHHHHEECCCCCCEEEEEEEHHHHHHCCC
>Mature Secondary Structure 
AETLAEAVSRRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAGEVKAKKDRIQTRSDWMK
HHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHHHH
IERERGISVVTSVMTFEYNDRVFNILDTPGHEDFADDTYRTLTAVDAAIMVIDAAKGIEP
HHHHCCHHHHHHHHHEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCC
RTLKLFEVCRMRDIPIITFINKMDRESRDPFEILDEVEEKLALDTAPITWPVGRAKSFCG
HHHHHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
AYNLAENTFRGNDTQVDGLSVNGPQAVADRLPENERKVFVEETELALEACRPFDRQAFLE
HHHHHHCCCCCCCCEECCEECCCHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCHHHHHH
GHMTPVFFGSALRNFGVRDLINALGDFAPPPRDQVADTRVVHAAEDKMTAFVFKIQANMD
CCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHCCCCEEEECCCCEEEEEEEEEECCC
PNHRDRIAFARICSGKLERGMKARLSRTGKLMGLTAPQFFFASQRQLADTAFAGDVVGIP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHCCCEEECC
NHGTLRIGDTLTEGENLVFQGVPNFSPEILRRVRLEDAMKAKKLKEALQQMAEEGVVQLF
CCCEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SPEDGSPAIVGVVGALQLDVLKERLMGEYGLPVSFEMSRFSVCRWISSDQPAEMDKFLNV
CCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHCC
KRGDIARDLDGDPVFLAQDAFSLRYESERFPAIKMVAIKEYHVAKAA
CCCCCCCCCCCCEEEEECCHHHHEECCCCCCEEEEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA