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The map label for this gene is nodL [H]

Identifier: 15887653

GI number: 15887653

Start: 296456

End: 297016

Strand: Direct

Name: nodL [H]

Synonym: Atu0304

Alternate gene names: 15887653

Gene position: 296456-297016 (Clockwise)

Preceding gene: 15887652

Following gene: 15887654

Centisome position: 10.43

GC content: 61.5

Gene sequence:

>561_bases
ATGTCCACTGAGCGGGAAAAGATGGCCGCGGGCGAATGGTACAGCTGCATGGGTGCCGAACTGGATATGCTGCGCTGGCA
TTCACGCCGGGCGGTTCACCAGCACAATACCATGCCGCCGGATGAGCGCGGCACCATCGGGCCGCTGCTGCGCGCACTTT
TTGCCTCCGTGGGCGAAGGTGCATTCATCGAGGCGCCTTTTCATTGCGCCTACGGTTTCAACATTACGCTCGGCAGGAAT
GCCTATCTCAATGCCGGCTGCACCATTCTCGATTCTGCGAAGGTCGCGATAGGCGACGGCGCGATGCTCGGCCCCGCCGT
GCAGATCTATTGTGCGGAACACCATCTTGATCCGGTTCCGCGCGCGCAGGGGATTGAGATTGCAAAGCCTGTCACGATCG
GCCGTGATGTCTGGATCGGCGGCGGAGCGATCATTCTGGCCGGTATCACCATCGGTGACGGTGCAATTGTCGGTGCCGGC
TCGGTGGTGACGCGCAATGTGCCGGCGCGTGCAACGGTCGTGGGAAATCCCGCCCGACCCATAAATCGTATAGCCGGCTG
A

Upstream 100 bases:

>100_bases
GATGCCGGCCGTTTTTCGCTGCGCTTCGATATTGTCGCCGTTCTGCCACGACGCCTGCCGCAGCACTTTATCGACGCATT
TTAAAGGGGAGGGGAAGGCA

Downstream 100 bases:

>100_bases
GATTGTCGGCACGTGGTGTAATAAATCTGACACGGAACTGTCAAAGAGGCGTCATCGAACCGCTCTATTCGCATCCTCAC
CCCAATAAATGGTGGAGAGG

Product: nodulation protein L

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MSTEREKMAAGEWYSCMGAELDMLRWHSRRAVHQHNTMPPDERGTIGPLLRALFASVGEGAFIEAPFHCAYGFNITLGRN
AYLNAGCTILDSAKVAIGDGAMLGPAVQIYCAEHHLDPVPRAQGIEIAKPVTIGRDVWIGGGAIILAGITIGDGAIVGAG
SVVTRNVPARATVVGNPARPINRIAG

Sequences:

>Translated_186_residues
MSTEREKMAAGEWYSCMGAELDMLRWHSRRAVHQHNTMPPDERGTIGPLLRALFASVGEGAFIEAPFHCAYGFNITLGRN
AYLNAGCTILDSAKVAIGDGAMLGPAVQIYCAEHHLDPVPRAQGIEIAKPVTIGRDVWIGGGAIILAGITIGDGAIVGAG
SVVTRNVPARATVVGNPARPINRIAG
>Mature_185_residues
STEREKMAAGEWYSCMGAELDMLRWHSRRAVHQHNTMPPDERGTIGPLLRALFASVGEGAFIEAPFHCAYGFNITLGRNA
YLNAGCTILDSAKVAIGDGAMLGPAVQIYCAEHHLDPVPRAQGIEIAKPVTIGRDVWIGGGAIILAGITIGDGAIVGAGS
VVTRNVPARATVVGNPARPINRIAG

Specific function: Acetyltransferase implicated in the O-acetylation of Nod factors [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=184, Percent_Identity=47.2826086956522, Blast_Score=170, Evalue=4e-44,
Organism=Escherichia coli, GI1786537, Length=179, Percent_Identity=36.3128491620112, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1788367, Length=130, Percent_Identity=29.2307692307692, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322243, Length=181, Percent_Identity=40.8839779005525, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 19582; Mature: 19451

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEREKMAAGEWYSCMGAELDMLRWHSRRAVHQHNTMPPDERGTIGPLLRALFASVGEG
CCCCHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCC
AFIEAPFHCAYGFNITLGRNAYLNAGCTILDSAKVAIGDGAMLGPAVQIYCAEHHLDPVP
EEEECCEEEEECCEEEECCCCEECCCEEEECCCEEEECCCCEECCEEEEEEECCCCCCCC
RAQGIEIAKPVTIGRDVWIGGGAIILAGITIGDGAIVGAGSVVTRNVPARATVVGNPARP
CCCCCEECCCEEECCEEEECCCEEEEEEEEECCCCEEECCCEEECCCCCEEEEECCCCCH
INRIAG
HHHHCC
>Mature Secondary Structure 
STEREKMAAGEWYSCMGAELDMLRWHSRRAVHQHNTMPPDERGTIGPLLRALFASVGEG
CCCHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCC
AFIEAPFHCAYGFNITLGRNAYLNAGCTILDSAKVAIGDGAMLGPAVQIYCAEHHLDPVP
EEEECCEEEEECCEEEECCCCEECCCEEEECCCEEEECCCCEECCEEEEEEECCCCCCCC
RAQGIEIAKPVTIGRDVWIGGGAIILAGITIGDGAIVGAGSVVTRNVPARATVVGNPARP
CCCCCEECCCEEECCEEEECCCEEEEEEEEECCCCEEECCCEEECCCCCEEEEECCCCCH
INRIAG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 0.018 {acetyl-CoA}} 62 {glucose}} 90 {maltose}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1558960; 8801423; 11481432 [H]