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The map label for this gene is mepA [H]

Identifier: 15887540

GI number: 15887540

Start: 188270

End: 189325

Strand: Reverse

Name: mepA [H]

Synonym: Atu0186

Alternate gene names: 15887540

Gene position: 189325-188270 (Counterclockwise)

Preceding gene: 15887548

Following gene: 15887539

Centisome position: 6.66

GC content: 63.83

Gene sequence:

>1056_bases
ATGACGGCGGGATCACCAAGAGTTTTGAAGGCAGCCCTGCTGGCGATGTCGGTCGTCTCGGCGCTGCAGTTGCAGATCGA
GACCGCATCGGCCGAAGACAGGCCCGCGAAAGAGGTGTTTGGCCACATGGCCCTGCCTTCTGCGGCGGCCTCTCAACCGA
TTGGCTCCTATGCCAAGGGCTGCCAGTCCGGCGCCATCGCCATGCCGACCGATGGTCCCGGCTGGCAGGCCATGCGGCTT
TCGCGCAACCGGCGTTGGGGCCAGCCGCAGCTGATTTCCTTCTTGGAGCGATTTTCGCAGGATGCGCAGAAGATCGGCTG
GCCGGGATTGCTGCTCGGCGATATTTCCCAGCCGCGCGGCGGCCCGATGCTGACCGGCCACGCCTCGCACCAGATCGGCC
TCGATGTCGACGTCTGGTGGCGGCCGATGCCCTCCCCCCGGCTGACCGTGGAGCAGCGCGAAACGGTGCCTTTCATCTCG
ATGCTGGACAAGAGCAAGTTCCTGACGGTCGACGACCGCAAATGGTCACCGCTCAATGCCCGTCTGGTGATGATGGCGGC
AAGTTACCCGCAGGTGGAGCGTGTCTTCGTCAACCCGGCCATCAAGCAGAAGCTCTGCCAGACATGGACCGGCGACCGCA
CCTTCATGGGCAAGATCAGGCCGATCTACGGCCATGACGAACATTTCCACATCCGTCTCGAATGCCCGCCCGGCGCGCCC
AACTGCAAACCGCAGGCGGAGGTCGGCAAAGGCGACGGCTGCGACAAGTCGCTTGCCTGGTGGTTCACCAAGGAACCATG
GGCGCCGCCGAAAAAGGACCCCAATGCCAAGCCGGTGAAACCGCGCCAGGTCATGGTCTCGGATTTGCCGGCGGCCTGCG
CTGCTGTTGCGGCAGCTCCTTCCGCCAATCCCGAGGGGATTGCGGCTCAGGCCTATTCCTCCTCCCCTGCCCAGGCCGTG
CGTCAGCAGAATGTGGAACAGGTCATTCAAAACGCCCCGGCGATCCAGCCACCGTCCGATATTCCGCTTCCGACCCAACG
TCCGACATTGAACTGA

Upstream 100 bases:

>100_bases
CCCCGCTTTGCGAGGGCCAGGCGAAACATGGTTTCAACAAAATCCAGTTCCAGTTTATTCTCGGGGCAAGAGAATCACCT
GATCGGACGGGCACGGTTAT

Downstream 100 bases:

>100_bases
AACAGAAATGAGGCATTGAACAGCTTCCCTTTTCAAATGGCGGCAAGCTGTTATAGAAGGCTTCAAATCGATTTAATTCT
TTGAGGACAGGCTGGGACCA

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTAGSPRVLKAALLAMSVVSALQLQIETASAEDRPAKEVFGHMALPSAAASQPIGSYAKGCQSGAIAMPTDGPGWQAMRL
SRNRRWGQPQLISFLERFSQDAQKIGWPGLLLGDISQPRGGPMLTGHASHQIGLDVDVWWRPMPSPRLTVEQRETVPFIS
MLDKSKFLTVDDRKWSPLNARLVMMAASYPQVERVFVNPAIKQKLCQTWTGDRTFMGKIRPIYGHDEHFHIRLECPPGAP
NCKPQAEVGKGDGCDKSLAWWFTKEPWAPPKKDPNAKPVKPRQVMVSDLPAACAAVAAAPSANPEGIAAQAYSSSPAQAV
RQQNVEQVIQNAPAIQPPSDIPLPTQRPTLN

Sequences:

>Translated_351_residues
MTAGSPRVLKAALLAMSVVSALQLQIETASAEDRPAKEVFGHMALPSAAASQPIGSYAKGCQSGAIAMPTDGPGWQAMRL
SRNRRWGQPQLISFLERFSQDAQKIGWPGLLLGDISQPRGGPMLTGHASHQIGLDVDVWWRPMPSPRLTVEQRETVPFIS
MLDKSKFLTVDDRKWSPLNARLVMMAASYPQVERVFVNPAIKQKLCQTWTGDRTFMGKIRPIYGHDEHFHIRLECPPGAP
NCKPQAEVGKGDGCDKSLAWWFTKEPWAPPKKDPNAKPVKPRQVMVSDLPAACAAVAAAPSANPEGIAAQAYSSSPAQAV
RQQNVEQVIQNAPAIQPPSDIPLPTQRPTLN
>Mature_350_residues
TAGSPRVLKAALLAMSVVSALQLQIETASAEDRPAKEVFGHMALPSAAASQPIGSYAKGCQSGAIAMPTDGPGWQAMRLS
RNRRWGQPQLISFLERFSQDAQKIGWPGLLLGDISQPRGGPMLTGHASHQIGLDVDVWWRPMPSPRLTVEQRETVPFISM
LDKSKFLTVDDRKWSPLNARLVMMAASYPQVERVFVNPAIKQKLCQTWTGDRTFMGKIRPIYGHDEHFHIRLECPPGAPN
CKPQAEVGKGDGCDKSLAWWFTKEPWAPPKKDPNAKPVKPRQVMVSDLPAACAAVAAAPSANPEGIAAQAYSSSPAQAVR
QQNVEQVIQNAPAIQPPSDIPLPTQRPTLN

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=254, Percent_Identity=36.6141732283465, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 38255; Mature: 38124

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGSPRVLKAALLAMSVVSALQLQIETASAEDRPAKEVFGHMALPSAAASQPIGSYAKG
CCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCCCHHHCCCCHHHHHH
CQSGAIAMPTDGPGWQAMRLSRNRRWGQPQLISFLERFSQDAQKIGWPGLLLGDISQPRG
HCCCCEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
GPMLTGHASHQIGLDVDVWWRPMPSPRLTVEQRETVPFISMLDKSKFLTVDDRKWSPLNA
CCEEECCCCCEECCEEEEEECCCCCCCEECCCCCCCHHHHHHCCCCEEEECCCCCCCCCC
RLVMMAASYPQVERVFVNPAIKQKLCQTWTGDRTFMGKIRPIYGHDEHFHIRLECPPGAP
EEEEEECCCCCCEEEEECHHHHHHHHHHCCCCEEEECCEEEECCCCCEEEEEEECCCCCC
NCKPQAEVGKGDGCDKSLAWWFTKEPWAPPKKDPNAKPVKPRQVMVSDLPAACAAVAAAP
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCC
SANPEGIAAQAYSSSPAQAVRQQNVEQVIQNAPAIQPPSDIPLPTQRPTLN
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAGSPRVLKAALLAMSVVSALQLQIETASAEDRPAKEVFGHMALPSAAASQPIGSYAKG
CCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHCCCCHHHCCCCHHHHHH
CQSGAIAMPTDGPGWQAMRLSRNRRWGQPQLISFLERFSQDAQKIGWPGLLLGDISQPRG
HCCCCEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
GPMLTGHASHQIGLDVDVWWRPMPSPRLTVEQRETVPFISMLDKSKFLTVDDRKWSPLNA
CCEEECCCCCEECCEEEEEECCCCCCCEECCCCCCCHHHHHHCCCCEEEECCCCCCCCCC
RLVMMAASYPQVERVFVNPAIKQKLCQTWTGDRTFMGKIRPIYGHDEHFHIRLECPPGAP
EEEEEECCCCCCEEEEECHHHHHHHHHHCCCCEEEECCEEEECCCCCEEEEEEECCCCCC
NCKPQAEVGKGDGCDKSLAWWFTKEPWAPPKKDPNAKPVKPRQVMVSDLPAACAAVAAAP
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCC
SANPEGIAAQAYSSSPAQAVRQQNVEQVIQNAPAIQPPSDIPLPTQRPTLN
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]