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The map label for this gene is phnJ [H]

Identifier: 15887532

GI number: 15887532

Start: 181722

End: 182624

Strand: Reverse

Name: phnJ [H]

Synonym: Atu0178

Alternate gene names: 15887532

Gene position: 182624-181722 (Counterclockwise)

Preceding gene: 15887533

Following gene: 159184199

Centisome position: 6.43

GC content: 60.02

Gene sequence:

>903_bases
ATGCTTAAAGAAGTTTCATTTGACGACGGGCTGGCTGCCTACAACTTCGCCTATCTGGACGAACAGACCAAGCGGATGAT
CCGCCGGGCGATCCTGAAAGCCATCGCCATTCCCGGTTACCAGGTTCCCTTCGCTTCCCGTGAAATGCCCATGCCCTATG
GCTGGGGCACCGGTGGCGTGCAGTTGACCGCCTCCATCCTCGGCCCTGATGATGTGCTGAAGGTCATCGACCAGGGCGCC
GACGACACGACCAATGCGGTTTCCATCCGTGCCTTTTTCCAGAAGGTGGCCGATGTGGCCGTCACCACCCGTACGAAGGA
CGCGACGATCATCCAGACGCGGCACCGCATTCCCGAAGAGGAACTGCATTCGGGTCAGGTACTGGTCTATCAGGTTCCCA
TCCCCGAGCCCCTACGCTTCCTCGAGCCGCGCGAGACCGAAACCCGTGTCATGCACGCACTGGAGGAGTATGGCCTGATG
CACGTCAAGCTTTACGAGGACATCGCCCGCAACGGCCGCATCTCCACCACCTATGCCTATCCGGTGAAGGTGGCCGGGCG
CTACGTGATGGACCCGTCGCCGACACCGAAATTCGACAATCCGAAAATGAATATGTCGGACGCCCTGCAACTGTTCGGCG
CCGGGCGCGAAAAGCGAATTTACGCCGTGCCGCCCTATACCGATGTCGTCAGCCTCGACTTCGAAGATCACCCCTTCGAA
ATCCAGCGCTTCGACAAGCCCTGCGCCCTGTGCGGCGGGGAGAATGTCTATCTCGACGAAGTGGTACTGGATGACAAGGG
CGGGCGCATGTTCGTCTGCTCCGATACCGACCATTGCGAAGACCGCCGTGCTCATGGCCACAAGGGCCATCTGCTGGCGG
ATGTGAAGGAGGCCGCAGAATGA

Upstream 100 bases:

>100_bases
CGCAAAAGACGGTCGATGCTGCAAAGACAGCAAAGAACACGGCTGCAAGAATTCCAATCTCCATGAGCTTGTACTCCTTT
TTTCACAAGGCTAACGCGCA

Downstream 100 bases:

>100_bases
GCGACGCACCGCTTCTGAAAGTCCGGGACGTTTCCAAATATTACGGCGACCGCGCCGGTTGCCGCAACGTCTCCTTCGAG
CTTTATCCGGGCGAGGTGCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MLKEVSFDDGLAAYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQLTASILGPDDVLKVIDQGA
DDTTNAVSIRAFFQKVADVAVTTRTKDATIIQTRHRIPEEELHSGQVLVYQVPIPEPLRFLEPRETETRVMHALEEYGLM
HVKLYEDIARNGRISTTYAYPVKVAGRYVMDPSPTPKFDNPKMNMSDALQLFGAGREKRIYAVPPYTDVVSLDFEDHPFE
IQRFDKPCALCGGENVYLDEVVLDDKGGRMFVCSDTDHCEDRRAHGHKGHLLADVKEAAE

Sequences:

>Translated_300_residues
MLKEVSFDDGLAAYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQLTASILGPDDVLKVIDQGA
DDTTNAVSIRAFFQKVADVAVTTRTKDATIIQTRHRIPEEELHSGQVLVYQVPIPEPLRFLEPRETETRVMHALEEYGLM
HVKLYEDIARNGRISTTYAYPVKVAGRYVMDPSPTPKFDNPKMNMSDALQLFGAGREKRIYAVPPYTDVVSLDFEDHPFE
IQRFDKPCALCGGENVYLDEVVLDDKGGRMFVCSDTDHCEDRRAHGHKGHLLADVKEAAE
>Mature_300_residues
MLKEVSFDDGLAAYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQLTASILGPDDVLKVIDQGA
DDTTNAVSIRAFFQKVADVAVTTRTKDATIIQTRHRIPEEELHSGQVLVYQVPIPEPLRFLEPRETETRVMHALEEYGLM
HVKLYEDIARNGRISTTYAYPVKVAGRYVMDPSPTPKFDNPKMNMSDALQLFGAGREKRIYAVPPYTDVVSLDFEDHPFE
IQRFDKPCALCGGENVYLDEVVLDDKGGRMFVCSDTDHCEDRRAHGHKGHLLADVKEAAE

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=273, Percent_Identity=77.2893772893773, Blast_Score=455, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 33741; Mature: 33741

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEVSFDDGLAAYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGV
CCCCCCCCCCCHHEEEHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
QLTASILGPDDVLKVIDQGADDTTNAVSIRAFFQKVADVAVTTRTKDATIIQTRHRIPEE
EEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCEEEHHHHCCCHH
ELHSGQVLVYQVPIPEPLRFLEPRETETRVMHALEEYGLMHVKLYEDIARNGRISTTYAY
HHCCCCEEEEECCCCCHHHHCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHCCCEEEEEEE
PVKVAGRYVMDPSPTPKFDNPKMNMSDALQLFGAGREKRIYAVPPYTDVVSLDFEDHPFE
EEEECCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCEEEECCCCCCCH
IQRFDKPCALCGGENVYLDEVVLDDKGGRMFVCSDTDHCEDRRAHGHKGHLLADVKEAAE
HHHCCCCEEEECCCCEEEEEEEEECCCCEEEEECCCCCCHHHHCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MLKEVSFDDGLAAYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGV
CCCCCCCCCCCHHEEEHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
QLTASILGPDDVLKVIDQGADDTTNAVSIRAFFQKVADVAVTTRTKDATIIQTRHRIPEE
EEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCEEEHHHHCCCHH
ELHSGQVLVYQVPIPEPLRFLEPRETETRVMHALEEYGLMHVKLYEDIARNGRISTTYAY
HHCCCCEEEEECCCCCHHHHCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHCCCEEEEEEE
PVKVAGRYVMDPSPTPKFDNPKMNMSDALQLFGAGREKRIYAVPPYTDVVSLDFEDHPFE
EEEECCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCEEEECCCCCCCH
IQRFDKPCALCGGENVYLDEVVLDDKGGRMFVCSDTDHCEDRRAHGHKGHLLADVKEAAE
HHHCCCCEEEECCCCEEEEEEEEECCCCEEEEECCCCCCHHHHCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]