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The map label for this gene is phnD [H]

Identifier: 15887527

GI number: 15887527

Start: 177492

End: 178397

Strand: Reverse

Name: phnD [H]

Synonym: Atu0173

Alternate gene names: 15887527

Gene position: 178397-177492 (Counterclockwise)

Preceding gene: 159184197

Following gene: 159184196

Centisome position: 6.28

GC content: 59.49

Gene sequence:

>906_bases
ATGTTGAAGAAAGTCCTTCTTTCGGCAGTCGCCCTTGGCGTTCTGGCCGGTTCCGCCATGGCTCAGGACGTCAAGGTTCT
GCGCATCGGTCTCGACGGTTCCGAAAACGAAGCCGACCAGATCCGCAACACCAAGTGCGTTGCCGATGGCCTCAAGGCTG
CAACCGGCGTTTCCGAAGTTCAGGTTTTCCCGTCGCCGGATTATAACGGCGTCATCCAGGGCCTGCTCGGCGGCACCATC
GACATCGCCTCCATGGGCGCATCCTCCTACGCCAAGATCGCTCTGGCCGACCCGAAGGCTGTCGACCCGATCCTGACCAC
GGCCGGCGCCGATGGTTCGACCGGTTATTACACGATCATGGTTGCCCGCAAGGACAGCGGCATCAAGACGCTGGCTGATG
CCAAGGGCAAGAAGATCGGCTTCGCCGACCCCGACTCCACCTCCGGCTTCCTCGTTCCGAACGTGGCTATCCCGAAGGAA
ACCGGCGTTCCGGTCAAGGAATACTTCTCCGAAACCGGTTTCGGCGGCGGCCATGAGAACCTCGTTCTCGCCGTTCTCGA
CAAGAAGTTCGACGTCGGCACCACCTTCGGTTCGGGCGTTGGCAAGTGGGAAGAAGGCTACACCGCCGGCAACCTCTACC
AGATGGTCAAGAAGGGCAATCTGGACATGGACGATATCGTTCAGGTCTGGAAGTCGCCGCTGATCCCGAACGGTCCGCTG
ATGGTCACCAACAAGCTCGGCGACGCGATGAAGCAGAAAGTCGAAGACTTCTTCATGGAACTGCCAAAGAAGGACCTCGC
ATGCTTCCAGGGCTTCACCCAGGGCAAGAACACCGCCTACATCAAGGTCGATCCGTCCTTCTACCAGACGATCATCGACG
CCCGTAAGTCCGTTATCGGCGGCTGA

Upstream 100 bases:

>100_bases
CTCGCCGGGCTCTGAGGAACCGCTCAGCATCGTTTGCGCCCGCGTCAAGGGCCGACATTTCACAACAAACTCCGGCTCGC
CGGGAAACAGGAGAAAGTCC

Downstream 100 bases:

>100_bases
TCCTCAGATCATATTCGGAAGCGGCGGTGCCTTGCGGCGTCGCCGCTTTTGCCAATAAAGGAAAAGCGGCATGGCGACCA
CACTGCACAATGGTACCCTA

Product: ABC transporter, substrate binding protein (phosphonate)

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MLKKVLLSAVALGVLAGSAMAQDVKVLRIGLDGSENEADQIRNTKCVADGLKAATGVSEVQVFPSPDYNGVIQGLLGGTI
DIASMGASSYAKIALADPKAVDPILTTAGADGSTGYYTIMVARKDSGIKTLADAKGKKIGFADPDSTSGFLVPNVAIPKE
TGVPVKEYFSETGFGGGHENLVLAVLDKKFDVGTTFGSGVGKWEEGYTAGNLYQMVKKGNLDMDDIVQVWKSPLIPNGPL
MVTNKLGDAMKQKVEDFFMELPKKDLACFQGFTQGKNTAYIKVDPSFYQTIIDARKSVIGG

Sequences:

>Translated_301_residues
MLKKVLLSAVALGVLAGSAMAQDVKVLRIGLDGSENEADQIRNTKCVADGLKAATGVSEVQVFPSPDYNGVIQGLLGGTI
DIASMGASSYAKIALADPKAVDPILTTAGADGSTGYYTIMVARKDSGIKTLADAKGKKIGFADPDSTSGFLVPNVAIPKE
TGVPVKEYFSETGFGGGHENLVLAVLDKKFDVGTTFGSGVGKWEEGYTAGNLYQMVKKGNLDMDDIVQVWKSPLIPNGPL
MVTNKLGDAMKQKVEDFFMELPKKDLACFQGFTQGKNTAYIKVDPSFYQTIIDARKSVIGG
>Mature_301_residues
MLKKVLLSAVALGVLAGSAMAQDVKVLRIGLDGSENEADQIRNTKCVADGLKAATGVSEVQVFPSPDYNGVIQGLLGGTI
DIASMGASSYAKIALADPKAVDPILTTAGADGSTGYYTIMVARKDSGIKTLADAKGKKIGFADPDSTSGFLVPNVAIPKE
TGVPVKEYFSETGFGGGHENLVLAVLDKKFDVGTTFGSGVGKWEEGYTAGNLYQMVKKGNLDMDDIVQVWKSPLIPNGPL
MVTNKLGDAMKQKVEDFFMELPKKDLACFQGFTQGKNTAYIKVDPSFYQTIIDARKSVIGG

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=257, Percent_Identity=30.7392996108949, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017797
- InterPro:   IPR005770 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 31776; Mature: 31776

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKVLLSAVALGVLAGSAMAQDVKVLRIGLDGSENEADQIRNTKCVADGLKAATGVSEV
CHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHCHHHHHHHHHHHCCCCCE
QVFPSPDYNGVIQGLLGGTIDIASMGASSYAKIALADPKAVDPILTTAGADGSTGYYTIM
EEECCCCHHHHHHHHHCCEEEEHHCCCCCCEEEEECCCCCCCHHHEECCCCCCCCEEEEE
VARKDSGIKTLADAKGKKIGFADPDSTSGFLVPNVAIPKETGVPVKEYFSETGFGGGHEN
EEECCCCCHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCCCCCC
LVLAVLDKKFDVGTTFGSGVGKWEEGYTAGNLYQMVKKGNLDMDDIVQVWKSPLIPNGPL
EEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCE
MVTNKLGDAMKQKVEDFFMELPKKDLACFQGFTQGKNTAYIKVDPSFYQTIIDARKSVIG
EEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCC
G
C
>Mature Secondary Structure
MLKKVLLSAVALGVLAGSAMAQDVKVLRIGLDGSENEADQIRNTKCVADGLKAATGVSEV
CHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHCHHHHHHHHHHHCCCCCE
QVFPSPDYNGVIQGLLGGTIDIASMGASSYAKIALADPKAVDPILTTAGADGSTGYYTIM
EEECCCCHHHHHHHHHCCEEEEHHCCCCCCEEEEECCCCCCCHHHEECCCCCCCCEEEEE
VARKDSGIKTLADAKGKKIGFADPDSTSGFLVPNVAIPKETGVPVKEYFSETGFGGGHEN
EEECCCCCHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCCCCCC
LVLAVLDKKFDVGTTFGSGVGKWEEGYTAGNLYQMVKKGNLDMDDIVQVWKSPLIPNGPL
EEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCE
MVTNKLGDAMKQKVEDFFMELPKKDLACFQGFTQGKNTAYIKVDPSFYQTIIDARKSVIG
EEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]