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The map label for this gene is mltB [H]

Identifier: 15887446

GI number: 15887446

Start: 96508

End: 97773

Strand: Reverse

Name: mltB [H]

Synonym: Atu0092

Alternate gene names: 15887446

Gene position: 97773-96508 (Counterclockwise)

Preceding gene: 15887447

Following gene: 15887444

Centisome position: 3.44

GC content: 59.16

Gene sequence:

>1266_bases
ATGCCACACCGATTTGCTACCAAGGCACTGGCCGCCATTCTTTCCGTTTTTTCAGCAACGACAGTTCTTGCACAGTCCGC
CCCGAAAGGTGCTGCTGCCGCCAAATATGATGCAGACTTCAATGCCTGGCTGAAAAAGGAAATCTGGCCGGAGGCCCGCA
AGGCCGGCATTTCCCAAGAGACGCTGGAGGCCACGCTCAGCGGTCTTTCCATCAGCTGGAACCTGCCCGATCTTGTCATT
CCCGGTCAGAAACCGCCGAAGGAACAGAGCCAGAGCCAGGCGGAATTCTCCTCGCCTGGCGCTTATTTCTCCGAAAAGCG
GTTACAGGGGCTTGCCGTTACCGGCCGTTCGCTTGCGGCCACGCACGCAGCGACGCTGCGCAGGATCGAGACGAAATATG
GTGTTCCCGGCGATATCGTCGTTGCGATCTGGGGCCGCGAATCCGGTTTTGGTCGGGCAAAATTGCCGCATTCGGTGGTC
GATGTGCTGGCCACGAAAGCCTATATGTCGACCCGCAAGGAGATGTTCCGCACCGAGCTGATCGATGCGCTGAAGATCGT
CGAAAGCGGCGATATAGCGGCTTCACGCATGATGGGCTCCTGGGCCGGCGCGCTTGGCCAGCCGCAATTCATGCCGTCGA
GCTATCTCAAATATGCCGTGGATTTCGATGGCGATGGCCACCGCGATATCTGGAATTCGGTGCCGGATGCACTGGCATCC
ATAGCGAACTATCTTTCCCAGCGCGGCTGGCAGCGCAATCGCGACTGGGGCTTCGAAGTCTCCATCCCCGCCAATGTTTC
CTGCGCGCAGGAAGGCCCTGATCTGGCCCGACCGGTGGCCGACTGGGCGAAGATGGGCATTACCCGCATTTCCGGAAAAG
CCTTTCCTGTCAATGACCTGACCGCCGACGGCATGATGCTGGTGCCGGCCGGGCGCCATGGGCCGGAATTCGTGGTGACG
CCGAATTTCTATGTCATCAAGGAATATAATAATTCTGACCTCTACGCGCTCTTCATTGGCAATCTCGCCGACCGCATCGC
CCATGGGGCGGGACCGTTCAAGGGGGAATGGGGCAATGTCGGGTCAATGCTGCGCTCCGACGTGCTGACCATGCAGAAGG
CTCTGGTCGCCAAGGGTTACGATGTCGGCAAGGCGGACGGGCTGGCGGGCTTCAAGACGCGCCGCTCACTCGGTGACTGG
CAGTCGAAAAACGGCCTTGCACCGACATGTTTTCCCGATGCGACACTGAAGGCGAAGCTGAAATAA

Upstream 100 bases:

>100_bases
GGTGAGCTTGATTATCTCGACGAGGGCACATTGACGGCGGCGCTTCGGGCGCGAACGACGATCTGAAGCCTTCCCTCCCC
ACCATCCAAGAGGAGACCGA

Downstream 100 bases:

>100_bases
TCCGTCTCCGGCCCATCGTCTTTTAATGCTGGTCGGTATGGGGCTTGTGAAAGATATCGTCGGTTGGCGGAATGGTCTGC
CGTTCGATCATCCGGTGCAC

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MPHRFATKALAAILSVFSATTVLAQSAPKGAAAAKYDADFNAWLKKEIWPEARKAGISQETLEATLSGLSISWNLPDLVI
PGQKPPKEQSQSQAEFSSPGAYFSEKRLQGLAVTGRSLAATHAATLRRIETKYGVPGDIVVAIWGRESGFGRAKLPHSVV
DVLATKAYMSTRKEMFRTELIDALKIVESGDIAASRMMGSWAGALGQPQFMPSSYLKYAVDFDGDGHRDIWNSVPDALAS
IANYLSQRGWQRNRDWGFEVSIPANVSCAQEGPDLARPVADWAKMGITRISGKAFPVNDLTADGMMLVPAGRHGPEFVVT
PNFYVIKEYNNSDLYALFIGNLADRIAHGAGPFKGEWGNVGSMLRSDVLTMQKALVAKGYDVGKADGLAGFKTRRSLGDW
QSKNGLAPTCFPDATLKAKLK

Sequences:

>Translated_421_residues
MPHRFATKALAAILSVFSATTVLAQSAPKGAAAAKYDADFNAWLKKEIWPEARKAGISQETLEATLSGLSISWNLPDLVI
PGQKPPKEQSQSQAEFSSPGAYFSEKRLQGLAVTGRSLAATHAATLRRIETKYGVPGDIVVAIWGRESGFGRAKLPHSVV
DVLATKAYMSTRKEMFRTELIDALKIVESGDIAASRMMGSWAGALGQPQFMPSSYLKYAVDFDGDGHRDIWNSVPDALAS
IANYLSQRGWQRNRDWGFEVSIPANVSCAQEGPDLARPVADWAKMGITRISGKAFPVNDLTADGMMLVPAGRHGPEFVVT
PNFYVIKEYNNSDLYALFIGNLADRIAHGAGPFKGEWGNVGSMLRSDVLTMQKALVAKGYDVGKADGLAGFKTRRSLGDW
QSKNGLAPTCFPDATLKAKLK
>Mature_420_residues
PHRFATKALAAILSVFSATTVLAQSAPKGAAAAKYDADFNAWLKKEIWPEARKAGISQETLEATLSGLSISWNLPDLVIP
GQKPPKEQSQSQAEFSSPGAYFSEKRLQGLAVTGRSLAATHAATLRRIETKYGVPGDIVVAIWGRESGFGRAKLPHSVVD
VLATKAYMSTRKEMFRTELIDALKIVESGDIAASRMMGSWAGALGQPQFMPSSYLKYAVDFDGDGHRDIWNSVPDALASI
ANYLSQRGWQRNRDWGFEVSIPANVSCAQEGPDLARPVADWAKMGITRISGKAFPVNDLTADGMMLVPAGRHGPEFVVTP
NFYVIKEYNNSDLYALFIGNLADRIAHGAGPFKGEWGNVGSMLRSDVLTMQKALVAKGYDVGKADGLAGFKTRRSLGDWQ
SKNGLAPTCFPDATLKAKLK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=227, Percent_Identity=34.3612334801762, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 45574; Mature: 45443

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHRFATKALAAILSVFSATTVLAQSAPKGAAAAKYDADFNAWLKKEIWPEARKAGISQE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHCCHHHHHCCCCHH
TLEATLSGLSISWNLPDLVIPGQKPPKEQSQSQAEFSSPGAYFSEKRLQGLAVTGRSLAA
HHHHHHHCCEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHCCEEECCCHHHH
THAATLRRIETKYGVPGDIVVAIWGRESGFGRAKLPHSVVDVLATKAYMSTRKEMFRTEL
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IDALKIVESGDIAASRMMGSWAGALGQPQFMPSSYLKYAVDFDGDGHRDIWNSVPDALAS
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHEEEEECCCCCCHHHHHHHHHHHHH
IANYLSQRGWQRNRDWGFEVSIPANVSCAQEGPDLARPVADWAKMGITRISGKAFPVNDL
HHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCHHEECCCCCCCCCC
TADGMMLVPAGRHGPEFVVTPNFYVIKEYNNSDLYALFIGNLADRIAHGAGPFKGEWGNV
CCCCEEEEECCCCCCEEEECCCEEEEEECCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCH
GSMLRSDVLTMQKALVAKGYDVGKADGLAGFKTRRSLGDWQSKNGLAPTCFPDATLKAKL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHCCCCCCCCCCCCCCEEECC
K
C
>Mature Secondary Structure 
PHRFATKALAAILSVFSATTVLAQSAPKGAAAAKYDADFNAWLKKEIWPEARKAGISQE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHCCHHHHHCCCCHH
TLEATLSGLSISWNLPDLVIPGQKPPKEQSQSQAEFSSPGAYFSEKRLQGLAVTGRSLAA
HHHHHHHCCEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHCCEEECCCHHHH
THAATLRRIETKYGVPGDIVVAIWGRESGFGRAKLPHSVVDVLATKAYMSTRKEMFRTEL
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IDALKIVESGDIAASRMMGSWAGALGQPQFMPSSYLKYAVDFDGDGHRDIWNSVPDALAS
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHEEEEECCCCCCHHHHHHHHHHHHH
IANYLSQRGWQRNRDWGFEVSIPANVSCAQEGPDLARPVADWAKMGITRISGKAFPVNDL
HHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCHHEECCCCCCCCCC
TADGMMLVPAGRHGPEFVVTPNFYVIKEYNNSDLYALFIGNLADRIAHGAGPFKGEWGNV
CCCCEEEEECCCCCCEEEECCCEEEEEECCCCCEEEEEHHHHHHHHHCCCCCCCCCCCCH
GSMLRSDVLTMQKALVAKGYDVGKADGLAGFKTRRSLGDWQSKNGLAPTCFPDATLKAKL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHCCCCCCCCCCCCCCEEECC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]