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The map label for this gene is truB

Identifier: 15887439

GI number: 15887439

Start: 87307

End: 88254

Strand: Reverse

Name: truB

Synonym: Atu0085

Alternate gene names: 15887439

Gene position: 88254-87307 (Counterclockwise)

Preceding gene: 15887440

Following gene: 15887438

Centisome position: 3.11

GC content: 61.29

Gene sequence:

>948_bases
ATGTCCAAACCACGTAAACAGCAACACCGCAAACCCAAAGGCCGTCCGATTTCGGGCTGGCTCATCCTCGACAAGCCGCT
CGATTTCGGCTCCACAGAGGCCGTTTCCAAGATCAAATGGCTGTTCAACGCCCAGAAGGCGGGCCACGCCGGTACGCTTG
ATCCGCTCGCTTCCGGCATGCTGCCGATCGCGCTCGGTGACGCCACCAAGACCGTGCCCTACGTCATGGACGGCCGGAAG
ATCTACGAATTTACCGTGACCTGGGGCGAACAGCGCGCGACGGACGATCTCGAGGGTGAGGTGGTCGAATCCTCTGACCA
GCGCCCGGAAGAGCAGGCGATCCGCGATATTCTGCCCAATTATACCGGCGTGATCATGCAGACCCCGCCGCAGTTTTCCG
CCATCAAAATCGCTGGCGAGCGCGCCTATGATCTGGCGCGTGATGGTGAGACGGTGGAAATTCCTGCACGCGAAGTAGAA
ATCCATCGCCTGACGCTGCTGGCCTGCCCGGACGCCGATACCGCGCATTTCGAAGTGGAATGTGGCAAGGGCACCTACGT
GCGGGCGCTGGCCCGCGACATGGGCCGCGATCTCGGGTGTTTCGGCCATATCTCGGAACTGCGCCGCACCATGGTCGCCC
CCTTCGGCGAAGACATGATGGTGCCCCTTGAAACCTTGACGGCGCTGGAAGCGATAGAAGATCGTGACGAACGGCTGGAA
GCACTGGATGCCTTCCTGATCGATACGGCCGAGGCGTTGTCCTCACTTCCCCGCCTCATCATCAACGATGATCAGGCGCA
CAGGCTGAAGATGGGCAACCCCATCCTGCTGCGTGGGCGCGATGCGCCAGCCAATCACCCGGAAGCCTATGCCACCGCGC
AAGGCAAACTGGTCGCCATCGGCGAGATCGGCGAAGGCGAGTTCAGGCCGAAGCGGGTTTTTGGCTAA

Upstream 100 bases:

>100_bases
TTTCGACAATTACCAGAAGATCGACGCGCTCCTGCGCTCGCCCGAAGTTCAGCGCGATCTCGGTCCTTCAAACGAAAAAG
ACGACGAACAGAACTGAAGC

Downstream 100 bases:

>100_bases
CCATACGGCGCGGAGGCGCTGTCAATCGCCTCTTTCAATTGCACCCGGAATAGTTTATAGGCAGCGCCAGCTTGGGCTTT
GCCTATGCTGCTGAATGGCC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSKPRKQQHRKPKGRPISGWLILDKPLDFGSTEAVSKIKWLFNAQKAGHAGTLDPLASGMLPIALGDATKTVPYVMDGRK
IYEFTVTWGEQRATDDLEGEVVESSDQRPEEQAIRDILPNYTGVIMQTPPQFSAIKIAGERAYDLARDGETVEIPAREVE
IHRLTLLACPDADTAHFEVECGKGTYVRALARDMGRDLGCFGHISELRRTMVAPFGEDMMVPLETLTALEAIEDRDERLE
ALDAFLIDTAEALSSLPRLIINDDQAHRLKMGNPILLRGRDAPANHPEAYATAQGKLVAIGEIGEGEFRPKRVFG

Sequences:

>Translated_315_residues
MSKPRKQQHRKPKGRPISGWLILDKPLDFGSTEAVSKIKWLFNAQKAGHAGTLDPLASGMLPIALGDATKTVPYVMDGRK
IYEFTVTWGEQRATDDLEGEVVESSDQRPEEQAIRDILPNYTGVIMQTPPQFSAIKIAGERAYDLARDGETVEIPAREVE
IHRLTLLACPDADTAHFEVECGKGTYVRALARDMGRDLGCFGHISELRRTMVAPFGEDMMVPLETLTALEAIEDRDERLE
ALDAFLIDTAEALSSLPRLIINDDQAHRLKMGNPILLRGRDAPANHPEAYATAQGKLVAIGEIGEGEFRPKRVFG
>Mature_314_residues
SKPRKQQHRKPKGRPISGWLILDKPLDFGSTEAVSKIKWLFNAQKAGHAGTLDPLASGMLPIALGDATKTVPYVMDGRKI
YEFTVTWGEQRATDDLEGEVVESSDQRPEEQAIRDILPNYTGVIMQTPPQFSAIKIAGERAYDLARDGETVEIPAREVEI
HRLTLLACPDADTAHFEVECGKGTYVRALARDMGRDLGCFGHISELRRTMVAPFGEDMMVPLETLTALEAIEDRDERLEA
LDAFLIDTAEALSSLPRLIINDDQAHRLKMGNPILLRGRDAPANHPEAYATAQGKLVAIGEIGEGEFRPKRVFG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=205, Percent_Identity=35.609756097561, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI215599015, Length=264, Percent_Identity=26.8939393939394, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4503337, Length=264, Percent_Identity=26.8939393939394, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI2367200, Length=317, Percent_Identity=35.9621451104101, Blast_Score=178, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17553978, Length=274, Percent_Identity=28.1021897810219, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=272, Percent_Identity=26.4705882352941, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=168, Percent_Identity=31.547619047619, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_AGRT5 (Q8UJ53)

Other databases:

- EMBL:   AE007869
- PIR:   AH2586
- PIR:   H97368
- RefSeq:   NP_353120.1
- ProteinModelPortal:   Q8UJ53
- SMR:   Q8UJ53
- STRING:   Q8UJ53
- GeneID:   1132123
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0085
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   ATKLIHL
- PhylomeDB:   Q8UJ53
- ProtClustDB:   PRK05389
- BioCyc:   ATUM176299-1:ATU0085-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 34828; Mature: 34697

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPRKQQHRKPKGRPISGWLILDKPLDFGSTEAVSKIKWLFNAQKAGHAGTLDPLASGM
CCCCCHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
LPIALGDATKTVPYVMDGRKIYEFTVTWGEQRATDDLEGEVVESSDQRPEEQAIRDILPN
EEEEECCCCCCCCEEECCCEEEEEEEEECCCCCCCCCCCCEECCCCCCCHHHHHHHHCCC
YTGVIMQTPPQFSAIKIAGERAYDLARDGETVEIPAREVEIHRLTLLACPDADTAHFEVE
CCEEEEECCCCCEEEEEECCHHHHHHCCCCEEEECCCEEEEEEEEEEECCCCCCEEEEEE
CGKGTYVRALARDMGRDLGCFGHISELRRTMVAPFGEDMMVPLETLTALEAIEDRDERLE
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ALDAFLIDTAEALSSLPRLIINDDQAHRLKMGNPILLRGRDAPANHPEAYATAQGKLVAI
HHHHHHHHHHHHHHHCCCEEECCCCHHEEECCCEEEEECCCCCCCCCCCEEECCCCEEEE
GEIGEGEFRPKRVFG
EECCCCCCCCCCCCC
>Mature Secondary Structure 
SKPRKQQHRKPKGRPISGWLILDKPLDFGSTEAVSKIKWLFNAQKAGHAGTLDPLASGM
CCCCHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
LPIALGDATKTVPYVMDGRKIYEFTVTWGEQRATDDLEGEVVESSDQRPEEQAIRDILPN
EEEEECCCCCCCCEEECCCEEEEEEEEECCCCCCCCCCCCEECCCCCCCHHHHHHHHCCC
YTGVIMQTPPQFSAIKIAGERAYDLARDGETVEIPAREVEIHRLTLLACPDADTAHFEVE
CCEEEEECCCCCEEEEEECCHHHHHHCCCCEEEECCCEEEEEEEEEEECCCCCCEEEEEE
CGKGTYVRALARDMGRDLGCFGHISELRRTMVAPFGEDMMVPLETLTALEAIEDRDERLE
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ALDAFLIDTAEALSSLPRLIINDDQAHRLKMGNPILLRGRDAPANHPEAYATAQGKLVAI
HHHHHHHHHHHHHHHCCCEEECCCCHHEEECCCEEEEECCCCCCCCCCCEEECCCCEEEE
GEIGEGEFRPKRVFG
EECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194