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The map label for this gene is lysR [H]

Identifier: 158423977

GI number: 158423977

Start: 2679172

End: 2680194

Strand: Reverse

Name: lysR [H]

Synonym: AZC_2353

Alternate gene names: 158423977

Gene position: 2680194-2679172 (Counterclockwise)

Preceding gene: 158423978

Following gene: 158423976

Centisome position: 49.91

GC content: 65.4

Gene sequence:

>1023_bases
GTGGATGCCTTGCGATGGCGGGCACGCGGGCCTAGCCTTGCGCCCGCTGCCGTGCATCGATCCGGGGGACAAGGGCTGGC
GATGAGGTCCGATACCGTTCGTCTGCCCCCTCTCAATGCCCTGCGGGTGTTCCACGCGGTCATGCGTCACGGCAGTTTCC
GCAGCGCCGCGGACGAACTGCTTGTCTCCCCGCAGGCTGTGGGCCAGCAGATCAAGCTTCTGGAAGACACCCTCGCGGTG
CCTCTGTTCGATCGACGGGGACGGGCGATCGAACCCACTGAAGAGGCCATTCTCCTCTCCCATTACGTGCAGAGTGGCTT
CGACGAGTTCCGCGAGGGGGTCAGGCGCATCTGCAAGGTGGGCCATCGCAACCGCATCAACCTGAATGCCAGCCCCTATT
TCGCGACGCGCTATCTCGTGGACCGGCTCGATCGATTCCGCGATCGTCTACCCGGTGCGGATATCCGGCTGAAGACGATG
GTGGAGCTCCCGGACTTTTCAGCCGACGAGGTGGATGCGGCCATTCAGTGGGGCTTCGGCCAGTGGCGGGATTATGAATC
GACGCTGCTGGTCCAGGATCCGAAGGTGATCTGCTGCTCACCGGCGCGGGCCTCGGCCCTGCGCAGTCCGCAGGACCTCA
GGACCGCACCGCTGCTCCATCTGGTGCTGGCAACGAACCTCTGGCCGCGCGTGTTGCGCCATCTCGGCGTGGATCCGGGC
GAGGTGCAGAAGGAAATCCAGTTCCACGACGCCGCCAGCATGCGCCGGGCGACGCTCTCCGGGCTCGGGATCGGCCTCAT
TTCCGTGCTCGATGCCCAGGAAGACCTCAAGGCGGGTCGACTCGTCGCGCCCTTCGGCCTTGATGCAATGGCGGGCATGG
ACCCGGCCGATGTGCCGGGCTTCTATCTCGTGCTGCCGCGCTCCAATCGGCGGCTCAAGAGCGTCGCGGCGTTCTGCGAA
TGGATCCTGTCGGAAGACTGGTCCCGCATGGAGCCGGATGCCCCTTTTCCGGCGGCGACTTAG

Upstream 100 bases:

>100_bases
GAACGGGAGCCCGCCCGGCTTAAGGCGTTCTGTCCGGTCGGCTCTCGCCTCACCGGGAGGCCTGAGACGCGCCCCTGCAC
GGAGGCGGGGGCACCTTCCC

Downstream 100 bases:

>100_bases
GGTGACCGCTCGGAGATAAGCAGAGGATCATTGGCGAGAGAAGCGGCAGGTCAGCAGCTTCCTTTATGCCTGTCGCGGCA
ACGCCTAAGGTGCTCGAGGG

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MDALRWRARGPSLAPAAVHRSGGQGLAMRSDTVRLPPLNALRVFHAVMRHGSFRSAADELLVSPQAVGQQIKLLEDTLAV
PLFDRRGRAIEPTEEAILLSHYVQSGFDEFREGVRRICKVGHRNRINLNASPYFATRYLVDRLDRFRDRLPGADIRLKTM
VELPDFSADEVDAAIQWGFGQWRDYESTLLVQDPKVICCSPARASALRSPQDLRTAPLLHLVLATNLWPRVLRHLGVDPG
EVQKEIQFHDAASMRRATLSGLGIGLISVLDAQEDLKAGRLVAPFGLDAMAGMDPADVPGFYLVLPRSNRRLKSVAAFCE
WILSEDWSRMEPDAPFPAAT

Sequences:

>Translated_340_residues
MDALRWRARGPSLAPAAVHRSGGQGLAMRSDTVRLPPLNALRVFHAVMRHGSFRSAADELLVSPQAVGQQIKLLEDTLAV
PLFDRRGRAIEPTEEAILLSHYVQSGFDEFREGVRRICKVGHRNRINLNASPYFATRYLVDRLDRFRDRLPGADIRLKTM
VELPDFSADEVDAAIQWGFGQWRDYESTLLVQDPKVICCSPARASALRSPQDLRTAPLLHLVLATNLWPRVLRHLGVDPG
EVQKEIQFHDAASMRRATLSGLGIGLISVLDAQEDLKAGRLVAPFGLDAMAGMDPADVPGFYLVLPRSNRRLKSVAAFCE
WILSEDWSRMEPDAPFPAAT
>Mature_340_residues
MDALRWRARGPSLAPAAVHRSGGQGLAMRSDTVRLPPLNALRVFHAVMRHGSFRSAADELLVSPQAVGQQIKLLEDTLAV
PLFDRRGRAIEPTEEAILLSHYVQSGFDEFREGVRRICKVGHRNRINLNASPYFATRYLVDRLDRFRDRLPGADIRLKTM
VELPDFSADEVDAAIQWGFGQWRDYESTLLVQDPKVICCSPARASALRSPQDLRTAPLLHLVLATNLWPRVLRHLGVDPG
EVQKEIQFHDAASMRRATLSGLGIGLISVLDAQEDLKAGRLVAPFGLDAMAGMDPADVPGFYLVLPRSNRRLKSVAAFCE
WILSEDWSRMEPDAPFPAAT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=301, Percent_Identity=36.2126245847176, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=31.1418685121107, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI157672245, Length=143, Percent_Identity=33.5664335664336, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=25.7627118644068, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081978, Length=302, Percent_Identity=25.1655629139073, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787128, Length=297, Percent_Identity=25.5892255892256, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786401, Length=249, Percent_Identity=26.1044176706827, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37847; Mature: 37847

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALRWRARGPSLAPAAVHRSGGQGLAMRSDTVRLPPLNALRVFHAVMRHGSFRSAADEL
CCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCEECCCHHHHHHHHHHHHCCCHHHHHHHH
LVSPQAVGQQIKLLEDTLAVPLFDRRGRAIEPTEEAILLSHYVQSGFDEFREGVRRICKV
HCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GHRNRINLNASPYFATRYLVDRLDRFRDRLPGADIRLKTMVELPDFSADEVDAAIQWGFG
CCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCC
QWRDYESTLLVQDPKVICCSPARASALRSPQDLRTAPLLHLVLATNLWPRVLRHLGVDPG
CCCCCCCEEEEECCCEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EVQKEIQFHDAASMRRATLSGLGIGLISVLDAQEDLKAGRLVAPFGLDAMAGMDPADVPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEECCCCCHHHCCCCCCCCCC
FYLVLPRSNRRLKSVAAFCEWILSEDWSRMEPDAPFPAAT
EEEEECCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MDALRWRARGPSLAPAAVHRSGGQGLAMRSDTVRLPPLNALRVFHAVMRHGSFRSAADEL
CCCCCCCCCCCCCCHHHHHCCCCCCEEECCCCEECCCHHHHHHHHHHHHCCCHHHHHHHH
LVSPQAVGQQIKLLEDTLAVPLFDRRGRAIEPTEEAILLSHYVQSGFDEFREGVRRICKV
HCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GHRNRINLNASPYFATRYLVDRLDRFRDRLPGADIRLKTMVELPDFSADEVDAAIQWGFG
CCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCC
QWRDYESTLLVQDPKVICCSPARASALRSPQDLRTAPLLHLVLATNLWPRVLRHLGVDPG
CCCCCCCEEEEECCCEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EVQKEIQFHDAASMRRATLSGLGIGLISVLDAQEDLKAGRLVAPFGLDAMAGMDPADVPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEECCCCCHHHCCCCCCCCCC
FYLVLPRSNRRLKSVAAFCEWILSEDWSRMEPDAPFPAAT
EEEEECCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]